Biomedical subjects

Tudorita Tumbar

Publications and source records attributed to Tudorita Tumbar.

3 recordsLinked to original sources

PubMed2006

Epithelial skin stem cells.

Major progress in understanding epithelial skin stem cells has been accomplished. This has been possible by developing new methods for labeling, tracking, isolating, and characterizing enriched populations of stem cells. This chapter summarizes in vivo and in vitro assays that are currently employed to analyze skin epithelial stem cells. Despite progress, the definition of a stem cell is currently a functional one. Unambiguous identification of a stem cell in intact tissue is still not possible. These limitations hamper molecular studies aimed at unraveling the cellular mechanisms operating in the stem cell compartment. This chapter emphasizes current methods for analyzing hair follicle stem cells, as opposed to other epithelial compartments, because the hair follicle has been most intensively studied up to date.

Animals↗

PubMed2004

Socializing with the neighbors: stem cells and their niche.

The potential of stem cells in regenerative medicine relies upon removing them from their natural habitat, propagating them in culture, and placing them into a foreign tissue environment. To do so, it is essential to understand how stem cells interact with their microenvironment, the so-called stem cell niche, to establish and maintain their properties. In this review, we examine adult stem cell niches and their impact on stem cell biology.

Animals↗

PubMed2003

Defining the epithelial stem cell niche in skin.

Many adult regenerative cells divide infrequently but have high proliferative capacity. We developed a strategy to fluorescently label slow-cycling cells in a cell type-specific fashion. We used this method to purify the label-retaining cells (LRCs) that mark the skin stem cell (SC) niche. We found that these cells rarely divide within their niche but change properties abruptly when stimulated to exit. We determined their transcriptional profile, which, when compared to progeny and other SCs, defines the niche. Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment.

Animals↗