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Biomedical subjects

Tomoyuki Haishi

Publications and source records attributed to Tomoyuki Haishi.

13 recordsLinked to original sources

Thawing of frozen vegetables observed by a small dedicated MRI for food research.

The thawing process for boiled and frozen edible vegetables was traced by a dedicated MRI for food research. The MRI system is small, with a 1.0-T static magnetic field, and can be placed in an ordinary research room with a light air conditioner. Images of green soybeans, broad beans, okra, asparagus and taro were measured by the spin-echo method (echo time=7 ms) with 0.1 or 0.2 s and 1 s repetition times. The images appeared along with the thawing time, and signals uniformly covered the sliced plane of the samples in the thawed condition. Information about the thawing process and tissue structures of the materials was obtained during transit thawing conditions. The thawing kinetics were examined with increased signal intensity, which were divided into two types. The signal increased linearly and saturated for okra and asparagus but exhibited convex curves for soybeans, broad beans and taro. The small MRI was stable, its handling was simple, and the internal structures of food materials could be accurately identified, although the grey-scale of the images was insufficient for determining precise textural fluctuations of tissue organization. We conclude that the devised MRI is useful for examining the quality of frozen foods and for developmental research into frozen foods.

Food Preservation↗

Graphic and movie illustrations of human prenatal development and their application to embryological education based on the human embryo specimens in the Kyoto collection.

Morphogenesis in the developing embryo takes place in three dimensions, and in addition, the dimension of time is another important factor in development. Therefore, the presentation of sequential morphological changes occurring in the embryo (4D visualization) is essential for understanding the complex morphogenetic events and the underlying mechanisms. Until recently, 3D visualization of embryonic structures was possible only by reconstruction from serial histological sections, which was tedious and time-consuming. During the past two decades, 3D imaging techniques have made significant advances thanks to the progress in imaging and computer technologies, computer graphics, and other related techniques. Such novel tools have enabled precise visualization of the 3D topology of embryonic structures and to demonstrate spatiotemporal 4D sequences of organogenesis. Here, we describe a project in which staged human embryos are imaged by the magnetic resonance (MR) microscope, and 3D images of embryos and their organs at each developmental stage were reconstructed based on the MR data, with the aid of computer graphics techniques. On the basis of the 3D models of staged human embryos, we constructed a data set of 3D images of human embryos and made movies to illustrate the sequential process of human morphogenesis. Furthermore, a computer-based self-learning program of human embryology is being developed for educational purposes, using the photographs, histological sections, MR images, and 3D models of staged human embryos.

Anatomy, Artistic↗

Low-field compact magnetic resonance imaging system for the hand and wrist in rheumatoid arthritis.

PURPOSE: To investigate the feasibility of an originally developed compact MRI system for evaluating rheumatoid arthritis (RA), and determine its advantages and disadvantages as an imaging modality for evaluating RA. MATERIALS AND METHODS: We prospectively studied 13 healthy controls with no clinical symptoms of arthritis, and 13 patients with hand and wrist pains (including pain from RA) with a 0.2 T permanent-magnet compact MR imager. All MR images were obtained while the subjects were in a sitting position. Coronal three-dimensional spin-echo T1-weighted images and coronal two-dimensional short tau inversion recovery (STIR) images were obtained with image matrix = 256 x 128 and field of view (FOV) = 20.48 cm. Plain radiograph findings and MRI findings of patients were compared. RESULTS: In three of the patients with suspected early RA (N = 7), early RA was evaluated based on STIR images. All RA patients showed morphologic or signal intensity changes that allowed an evaluation of RA from MR findings. Four of five RA patients showed high signal intensity on STIR images in the wrist, proximal interphalangeal (PIP) joint, or metacarpophalangeal (MCP) joint that suggested synovitis. Multiple erosions in the hand and wrist were seen in four RA patients, with low signal intensity on T1-weighted images. CONCLUSION: RA was correctly evaluated, and early RA could be identified with the compact MRI system. However, the current system has limitations, such as the nonselective STIR sequence used and magnetic field inhomogeneity.

Adult↗

Imaging living mice using a 1-T compact MRI system.

PURPOSE: To determine the feasibility of imaging living mice with a 1-T compact MRI system and investigate appropriate imaging techniques for use in routine animal experiments. MATERIALS AND METHODS: An MRI system consisting of a 1-T permanent magnet and compact console was used. Images of the entire trunks of living mice were obtained on the system using a T1-weighted three-dimensional fast low-angle shot (3D FLASH) sequence, and image quality was evaluated in relation to imaging techniques. RESULTS: Restraint of respiratory motion improved the image quality. Decreasing the slice thickness reduced artificial inhomogeneity in signal intensity (SI). Substantial effects of TR and FA on image quality were also demonstrated. With the determined techniques, images covering the entire trunk with a voxel size of 0.26x0.26x0.52 mm were acquired in an acquisition time of five minutes 28 seconds and a total experiment time of <20 minutes, and various organs and subcutaneous tumors were clearly visualized. CONCLUSION: The compact MRI system provides images of living mice with acceptable quality in a reasonable time. Considering its convenience, it appears to be suitable for use in routine mouse experiments.

Animals↗

Development of an ESR/MR dual-imaging system as a tool to detect bioradicals.

A system combining electron spin resonance imaging (ESRI) with another imaging modality capable of enabling visualization of the distribution of bioradicals on an anatomical map of the specimens would be a superior biomedical imaging system. We describe the development of an ESR/MR dual-imaging system with one permanent magnet and the biomedical applications of this system. The magnetic circuit developed for the ESR/MR dual-imaging system consisted of the permanent magnet made of Fe-Nd-B, pole pieces, and poke. The permanent magnet was installed on the MR side only, and the ESR side was made of pole pieces only. The magnetic field was adjusted to 0.5T at MR and to 0.042T at ESR. The overall dimensions of the magnet developed for the ESR/MR imaging system were 460 (W)x440 (D)x460 (H) mm, and it weighed 220 kg. The distance of each center for the magnet for ESR and MR imaging could be set as close as 200 mm. The entire ESR/MR imaging system can be installed in a common laboratory without magnetic shielding. MR images of plants (myoga) and small animals (mice and rats) were successfully acquired with or without ESR operation. ESR spectra of nitroxyl spin probes were also measured, even with MRI operation. ESR signals of triarylmethyl derivatives with narrow line-width (0.026 mT) were observed in living mice while MRI was operating. The ESR/MR imaging dual functions work properly with no electric or magnetic interference. The ESR/MR dual images demonstrate that this system enables visualization of the distribution of bioradicals on the anatomical map of the object.

Animals↗

Network images of drainage channels in sea spray icing by MR microscopy.

An MR imaging system was developed to visualize brine drainage channels in sea spray icing. Brine pockets trapped in spray-ice matrices during ice growth are structural features of sea spray icing. Brine in the spray ice sample had drained out; therefore, using a suction pump, we filled the air gaps in the drainage channels with dodecane. In the experiments, 0.5-1.0 h was necessary to accumulate signals sufficient to obtain a 3-D micro-image; the image matrix comprised 128(3) voxels (each voxel was 200 microm per side). The MIP view showed that sea spray icing has a developed drainage-channel network structure.

Alkanes↗

Trabecular bone volume fraction measurements of a large number of subjects using a compact MRI.

Trabecular bone volume fraction (TBVF) and speed of sound (SOS) were measured for the right calcanei of 416 female volunteers. The TBVF was measured with a compact MRI developed in our laboratory. The SOS was measured with a commercial quantitative ultrasound system. It was observed that the correlation coefficient between TBVF and SOS and that between TBVF and age varied depending on the location of region of interest (ROI) in the calcaneus. As a result, an optimum circular ROI with a diameter of 20 mm was determined so that the correlation coefficients were maximized. In the optimum ROI, transverse relaxation time (T2) of the bone marrow protons of the calcaneus was found to be concentrated in a narrow range over the subjects. This result suggested that a 50% scan time reduction in the TBVF measurements could be made by skipping the T2 correction procedure.

Adolescent↗

Development of a compact mouse MRI using a yokeless permanent magnet.

A compact mouse MRI has been developed using a 1.0T yokeless permanent magnet and portable MRI console. The entire system was installed in a space measuring 2 m x 1 m. The imaging region was the cylindrical volume (35 mm diameter, 50 mm length) at the center of the magnet and was used for whole-brain or body imaging of mice. Whole-brain imaging took less than 90 min for T1- and T2-weighted 3D images with 2-mm slice thickness and 200-microm in-plane resolution. Body imaging took less than 30 min for T1-weighted spin-echo and FLASH 3D images with 0.5- to 1.0-mm slice thickness and 250- to 300-microm in-plane resolution. In addition to the compactness of the system, the mouse MRI has several advantages over high-field superconducting animal MRI systems in its accessibility to the specimen, similarity to clinical MRI in image contrast, capacity for biological isolation, and maintenance. The results obtained demonstrate the potential of this new system for routine imaging in biomedical laboratories.

Animals↗

Development of a compact MRI system for trabecular bone volume fraction measurements.

A compact MRI system for measuring trabecular bone volume fraction (TBVF) of the calcaneus was developed with the use of a 0.21 T permanent magnet and portable MRI console. The entire system weighed < 600 kg and was installed in a 2 m x 2 m space. Two cross-sectional spin-echo images of a heel acquired with external reference phantoms (total measurement time = 5 min) were used to quantify the TBVF of the calcaneus. The linearity and reproducibility of the measurements were evaluated by means of proton density-adjusted phantoms. Comparative measurements with quantitative ultrasound (QUS) in groups of healthy female volunteers showed a relatively high positive correlation (R(2) = 0.4539, 0.2693) between TBVF and the speed of sound (SOS). These results demonstrate the potential of this new system for measuring bone density. Magn Reson Med 52:440-444, 2004.

Adolescent↗

MR microscopy of the articular cartilage with a 1.0T permanent magnet portable MR system: preliminary results.

The present study was designed to evaluate the use of a 1.0T portable permanent magnet MR system in obtaining microscopic MR images of the hyaline cartilage in vitro. A clear laminar appearance was demonstrated with this system. In addition, it was possible to demonstrate cartilage surface irregularity, a decrease in cartilage thickness, and T2 alteration by proteoglycan depletion following up to 12 hours of trypsin treatment. In summary, the portable MR system is useful for investigating cartilage in vitro.

Animals↗

Super-parallel MR microscope.

A super-parallel MR microscope in which multiple (up to 100) samples can be imaged simultaneously at high spatial resolution is described. The system consists of a multichannel transmitter-receiver system and a gradient probe array housed in a large-bore magnet. An eight-channel MR microscope was constructed for verification of the system concept, and a four-channel MR microscope was constructed for a practical application. Eight chemically fixed mouse fetuses were simultaneously imaged at the 200 micro m(3) voxel resolution in a 1.5 T superconducting magnet of a whole-body MRI, and four chemically fixed human embryos were simultaneously imaged at 120 micro m(3) voxel resolution in a 2.35 T superconducting magnet. Although the spatial resolutions achieved were not strictly those of MR microscopy, the system design proposed here can be used to attain a much higher spatial resolution imaging of multiple samples, because higher magnetic field gradients can be generated at multiple positions in a homogeneous magnetic field.

Anatomy, Cross-Sectional↗

MR microscopy of articular cartilage at 1.5 T: orientation and site dependence of laminar structures.

OBJECTIVE: To evaluate MR microscopic images of normal-appearing porcine hyaline cartilage ( n=15) in vitro obtained with an MR microscope using an independent console system (MRMICS) at 1.5 T. DESIGN AND RESULTS: The MRMICS is a portable imaging system consisting of a radiofrequency system, gradient power supplies and a personal computer. The images from the MRMICS showed a laminar structure of porcine cartilage similar to the structure demonstrated with other MR imaging techniques. The laminar structures of the articular cartilage, were, however heterogeneous in respect of signal intensity and thickness, which varied according to the site resected. The MR laminar appearance was most comparable to the staining with Masson's trichrome for collagen. CONCLUSION: MRMICS is a useful add-on system for obtaining microscopic MR images of articular cartilage in vitro.

Animals↗

Three-dimensional snow images by MR microscopy.

MR microscopy technique was introduced to visualize and quantify the three-dimensional structure of snowpack. Since the NMR signal from the ice was week, we looked at the air space instead filling with dodecane or aniline doped with iron acetylacetonate. Four types of snow were tested: ice spheres, large rounded poly crystals, small rounded mono-crystals and depth hoar crystals. A specific specimen-cooling system was developed to keep the temperature below 0 degrees C. In the experiments 0.5 to 2 h were necessary to accumulate the signals enough to obtain a 3D micro-image; the image matrix 128(3), voxel size (200 microm)3 or 256(3) (120 microm)3. Comparison with the 2D data using the conventional section plane method was also carried out and MR microscopy is proved to be a very useful method to visualize the microstructure of snowpack.

Ice↗