Biomedical subjects
Publications and source records attributed to Ting Fu.
Because of the difference in organic density, a simple and effective method for exponent restraint of active edge is applied to X-ray image of bone. And it is easy to use histogram method to get the active image of bone. At last, by associating it with Canny method, the edge of bone can be abstracted from a complex X-ray image.
Iterative weighted sparse decomposition is a method to determine the weight coefficients of the minimum l1 norm optimization in accord with the two-scale relationship of the white noise in the multiresolution wavelet structure and to gradually suppress the influence of the strong noises through an iteration operation. The single-trial Visual Evoked Potential(VEP) estimation results confirmed the effectiveness of the method, and thus supported the viewpoint that the results of the single VEP estimation were different.
OBJECTIVE: To examine the potential function of NMDA receptor NR2A subunit C-terminus in assembling and surface expression of the receptor in HEK293 cells. METHODS: Five vectors GFP- NR2ADeltaC1- DeltaC5 were constructed for expressing N-terminally GFP-tagged NR2A with C-terminal deletion at different regions by using conventional techniques of molecular cloning. The deleted region for NR2ADeltaC1-Delta C5 was 897L-1017S, 1024D-1142P, 1149D-1347G, 1354S-1464V, and 897L-1464V. These plasmids were transfected alone or co-transfected with NR1-1a into HEK293 cells. The surface NMDA receptors were immuno-stained using rabbit antibody against GFP and Cy3 conjugated secondary antibody in living cells. RESULT: The vectors GFP-NR2ADeltaC1-DeltaC5 were generated and all of them expressed GFP fluorescence in the transfected cells. Surface NMDA receptors were detected by immuno-labeling with anti-GFP in the cells co-transfected by NR1-1a and any one of GFP-NR2ADeltaC1-DeltaC5. However, no surface expression of NR2A proteins was found in the transfected cells with any one of these plasmids alone. CONCLUSION: Within the region downstream from the 897L of NR2A subunit, neither a particular domain directly interacted with ER retention domain in NR1-1a C1 cassette, nor that determining ER retention of NR2A subunit itself has been found, indicating that more complicated mechanisms might exist in which the subunit assembling and targeting to plasma membrane of NMDA receptors undergo.