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Biomedical subjects

Tetsuo Nishikawa

Publications and source records attributed to Tetsuo Nishikawa.

41 records · Page 3Linked to original sources
PubMed2002

Successful treatment of listerial brain abscess: a case report and literature review.

Listerial brain abscess is extremely rare; only two cases have been reported in Japan. We encountered a female patient with immunoblastic lymphadenopathy, who developed listerial brain abscess after 8 years of treatment with antineoplastic agents and corticosteroids. Brain MRI revealed multiple space occupying lesions, suggesting abscesses which were possibly caused by hematogenous spread of the bacteria. Immediate blood culture enabled early diagnosis, and she entered into complete remission with high-dose ampicillin. Blood culture and brain imaging seem to play a crucial role in making an early diagnosis, and the administration of high dose of antibiotics is recommended for improvement of this disease.

Brain Abscess↗
PubMed2002

Database and analysis system for cDNA clones obtained from full-length enriched cDNA libraries.

We have developed an efficient sequence-analysis system and a database system for clones obtained from full-length enriched cDNA libraries made by using the oligo-capping method. We developed a semi-automatic analysis system for 5'- and 3'-end sequences. It pre-processes raw sequences (vector cut and accurate-sequence region extraction), clusters the sequences, searches for similarities through public databases, annotates completeness of clones and analyzes the ORFs in the sequences. Newly developed or improved programs are used in each step. A new program, ESTiMateFull is used to evaluate and to predict the sequence-fullness based on comparisons with mRNA and EST sequences, respectively. The ATGpr program is used to predict sequence-fullness based on statistical information. The combination of full-length enriched cDNA clones and ATGpr fullness prediction resulted in 70% accuracy in the specificity and the sensitivity of the fullness predictions. For the ORFs predicted by the ATGpr, the signal peptides are predicted and a motif search is performed by our new system. We also developed a program that assembles our sequences with dbEST sequences and developed a system to retrieve clones by the characteristics of the ORFs. As keywords, combination of various results of the analyses can be used for retrieval. And various results such as ORF features and database search results can be shown on the same screen by multiple displays. Full-length clones having interesting functions can thus be retrieved efficiently by using this system.

Amino Acid Sequence↗
PubMed2002

Titanium-alloy particles induced cyclooxygenase-2 in human macrophage-like cells in vitro.

Prostaglandin E2 (PGE2) has been reported to be an important mediator in aseptic loosening and/or periprosthetic osteolysis in total joint arthroplasties. Previous studies have reported that human macrophages stimulated with particles in vitro release PGE2. However, they have not shown the expression of cyclooxygenases (COX) which are rate-limiting enzymes of PGE2 synthesis. We hypothesized that PGE2 production by activated macrophages is dependent upon the induction of COX-2, not upon the constitutive isozyme, COX-1. When we evaluated the expression of COX-1 and COX-2 in titanium-alloy particle-stimulated human macrophages, the expression of COX-2 mRNA was up-regulated by the particles, on the other hand, the expression of COX-1 mRNA was not affected. In addition, NS-398, a COX-2 inhibitor drastically suppressed up-regulated PGE2 production by the particles. These results provide strong evidence that PGE2 production by particle-stimulated macrophages is dependent upon the induction of COX-2, not COX-1.

Alloys↗