Biomedical subjects

Tao Sun

Publications and source records attributed to Tao Sun.

61 records · Page 4Linked to original sources

PubMed2002

[Primary lymphoma of the spleen: clinical analysis of 23 cases].

OBJECTIVE: To investigate the best diagnostic and therapeutic method for primary lymphoma of the spleen. METHODS: Clinicopathologic features of 23 patients treated from January 1956 to August 1999 were analyzed retrospectively. RESULTS: All patients but one for exploration only (96%) underwent resection of the tumor. They accepted chemotherapy after operation. 23 patients were confirmed pathologically. B-cell type non-Hodgkin's lymphoma was noted in 21 patients and T-cell letion in 2. According to Ahman's staging, 9 patients belonged to stage I, 8 stage II, and 6 stage III. The 5-year survival rates were 50%, 40% and 16% respectively. CONCLUSIONS: The diagnosis of splenic lymphoma is dependent mainly on B-ultrasound examination and CT scanning. Splenectomy combined with chemotherapy may provide optimum therapy for patients with splenic lymphoma.

Adult↗

PubMed2002

[The protective effect of bicyclol on ischemia-reperfusion induced kidney injury in rats].

AIM: To investigate the protective effect of bicyclol on kidney injury induced by ischemia-reperfusion in rats. METHODS: Bicyclol was orally administered to rats at doses of 50 and 200 mg.kg-1 before ischemia-reperfusion injury to evaluate the influence of bicyclol on the formation of MDA and BUN in serum, the content of GSH and the activity of GST in kidney, as well as kidney mitochondria membrane fluidity in ischaemia-reperfusion rats. RESULTS: Bicyclol given orally at doses of 50 and 200 mg.kg-1 was shown to significantly decrease the increment of MDA and BUN in serum and protect the GSH depletion in kidney. Bicyclol was also shown to induce kidney GST and ameliorate the decrease of mitochondria membrane fluidity. The protective effects of bicyclol on kidney injury induced by ischemia-reperfusion are dose-dependent. CONCLUSION: The protective action of bicyclol on kidney injury induced by ischemia-reperfusion may be attributed to its induction of kidney GSH and the GST, stabilization on mitochondria membrane and the inhibition on lipid peroxidation.

Animals↗

PubMed2002

[Endoscopic transnasal orbital decompression for dysthyroid orbitopathy].

OBJECTIVE: To evaluate the effect of endoscopic transnasal orbital decompression for patients with dysthyroid orbitopathy. METHODS: Nine cases (15 eyes) of dysthyroid orbitopathy were included in this study. All patients were treated by transnasal endoscopic orbital decompression. RESULTS: The follow-up ranged from 6 months to 2 years. Proptosis measured' by exophthalmoter reduced from (21.93 +/- 1.49) mm to (16.87 +/- 1.25) mm after operation. The visual acuity improved from 0.57 +/- 0.12 to 0.69 +/- 0.12, the palperbral fissures reduced from (11.07 +/- 1.44) mm to (8.20 +/- 1.15) mm. Postoperatively, the orbital pressure was significantly decreased as compared with the preoperative result (P < 0.001). Diplopia was cured in 3 of 7 cases. CONCLUSION: Endoscopic transnasal orbital decompression is an effective method for the treatment of dysthyroid orbitopathy.

Adult↗

PubMedDate not supplied

Self-organization of skin cells in three-dimensional electrospun polystyrene scaffolds.

Much research in tissue engineering focuses on the synthesis of complex three-dimensional polymer scaffolds containing functional biomolecules to which cells are introduced. Typical scaffolds for skin tissue engineering are macroscopically porous with struts or fibers approximately 10 microm thick at a packing fraction of approximately 0.1. We made a polystyrene scaffold without cell signaling or spatial information by electrospinning and studied the growth of skin fibroblasts, keratinocytes, and endothelial cells, as single and cocultured populations in the presence and absence of fetal calf serum. In the absence of serum, keratinocytes, fibroblasts, and endothelial cells did not grow when cultured alone. However, when fibroblasts were cocultured with keratinocytes and endothelial cells, expansion of keratinocytes and endothelial cells occurred even in the absence of serum. Furthermore, cells displayed native spatial three-dimensional organization when cultured at an air-liquid interface, even when all three cell types were introduced at random to the scaffold. This study shows that coculture with fibroblasts enables keratinocytes and endothelial cells to proliferate without serum, but also to self-organize according to the native epidermal-dermal structure given the symmetry-breaking field of an air-liquid interface.

Biocompatible Materials↗

PubMedDate not supplied

Development of a closed bioreactor system for culture of tissue-engineered skin at an air-liquid interface.

A bioreactor has been developed for the production of tissue-engineered skin at an air-liquid interface for clinical and experimental use. In this closed system, scaffold and bioreactor sterilization, cell seeding, and medium perfusion were all performed with a peristaltic pump. Natural and synthetic dermal substitutes were seeded directly with skin cells without opening the bioreactor and fed either by continuous medium perfusion or by batch-feed. The system was validated by monoculture of human dermal fibroblasts and keratinocytes and the coculture of both cell types in acellular human dermis, Azowipes, electrospun polystyrene, and an electrospun composite of polystyrene and poly-DL-lactide fibers. A comparison was made of culture at an air-liquid interface versus submerged culture and of medium change by continuous perfusion versus batch-feed. Fibroblast and endothelial cells showed greater viability under submerged rather than air-liquid conditions whereas keratinocytes favored culture at an air-liquid interface as did cocultured keratinocytes and fibroblasts. Total cellular viability for reconstructed skin with keratinocytes and fibroblasts was greatest with continuous perfusion rather than batch-feed and with electrospun scaffolds compared with acellular human dermis. The bioreactor could also be easily configured to give replicate small areas for experimental use or one continuous area of construct for clinical use.

Air↗

PubMedDate not supplied

Developments in xenobiotic-free culture of human keratinocytes for clinical use.

We have recently reported that irradiated human fibroblasts can be used as a feeder layer, to expand keratinocytes under serum-free conditions, on a chemically defined plasma polymer surface developed for the culture and transfer of keratinocytes for clinical use. While this is a significant advance in developing a serum-free keratinocyte culture approach, the need to irradiate fibroblasts to growth-arrest them and prevent them from overgrowing the keratinocytes introduces another small, but potential, risk for the patient. The aim of the present study was to develop conditions for the coculture of normal human keratinocytes with nonirradiated normal human fibroblasts under serum-free conditions. We examined the fibroblast/keratinocyte relationship on three separate surfaces: tissue culture plastic, non-tissue culture plastic, and a plasma polymer surface designed for clinical use. We report that it is possible to achieve rapid and successful expansion of human keratinocytes under serum-free conditions on all three surfaces providing one uses a keratinocyte-friendly media, a minimum seeding density of keratinocytes, and a ratio of fibroblasts to keratinocytes that does not exceed 1:1. These results provide us with a rapid laboratory expansion of proliferative human keratinocytes, under completely defined culture conditions, without any xenobiotic cells (mouse fibroblasts) or material (bovine serum), for the treatment of patients with extensive skin loss.

Acrylates↗

PubMedDate not supplied

Signal frequency based self-tuning fuzzy controller for semi-active suspension system.

A new kind of fuzzy control scheme, based on the identification of the signal's main frequency and the behavior of the ER damper, is proposed to control the semi-active suspension system. This method adjusts the fuzzy controller to achieve the best isolation effect by analyzing the main frequency's characters and inspecting the change of system parameters. The input of the fuzzy controller is the main frequency and the optimal damping ratio is the output. Simulation results indicated that the proposed control method is very effective in isolating the vibration.

Algorithms↗