Biomedical subjects

Tae-Kwang Oh

Publications and source records attributed to Tae-Kwang Oh.

At least 55 records · Page 3Linked to original sources

PubMed2005

Photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the Yellow Sea in Korea.

A Gram-negative, motile, non-spore-forming, pleomorphic and lipolytic bacterial strain, M37T, was isolated from an intertidal sediment of the Yellow Sea in Korea. This organism grew optimally at 25-28 degrees C and in the presence of 1-2 % NaCl. It did not grow without NaCl or in the presence of more than 6 % NaCl. Strain M37T was characterized chemotaxonomically by having Q-8 as the predominant respiratory lipoquinone and C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH and C(16 : 0) as the major fatty acids. The DNA G+C content was 47 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain M37T within the clade comprising Photobacterium species, forming a coherent cluster with the type strains of Photobacterium profundum and Photobacterium indicum (16S rRNA gene similarity levels of 97.5-98.0 %). The mean DNA-DNA relatedness levels between strain M37T and P. profundum JCM 10084T and P. indicum DSM 5151T were in the range 12-15 %. Similarities between 16S rRNA gene sequences of strain M37T and those of the type strains of the other Photobacterium species ranged from 93.9 % (with Photobacterium fischeri) to 96.2 % (with Photobacterium phosphoreum). On the basis of phenotypic properties and phylogenetic and genomic distinctiveness, strain M37T (=KCTC 10562BPT=DSM 16190T) should be placed in the genus Photobacterium as a novel species, for which the name Photobacterium lipolyticum sp. nov. is proposed.

Bacterial Typing Techniques↗

PubMed2005

Sphingopyxis flavimaris sp. nov., isolated from sea water of the Yellow Sea in Korea.

A Gram-negative, motile, yellow-pigmented, slightly halophilic bacterial strain, SW-151T, was isolated from sea water of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. The isolate grew optimally at 30 degrees C and in the presence of 2-3 % NaCl. Strain SW-151T was characterized chemotaxonomically as having Q-10 as the predominant respiratory lipoquinone and C(18 : 1)omega7c, C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH and C(17 : 1)omega6c as the major fatty acids. Sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. The DNA G+C content was 58 mol%. 16S rRNA gene sequence analysis showed that strain SW-151T joins the evolutionary radiation enclosed by the genus Sphingopyxis. Similarities between the 16S rRNA gene sequences of strain SW-151T and the type strains of Sphingopyxis species ranged from 92.3 to 94.3 %, which is low enough to categorize strain SW-151T as a species distinct from previously described Sphingopyxis species. On the basis of phenotypic properties and phylogenetic distinctiveness, strain SW-151T (=KCTC 12232T=DSM 16223T) should be classified as a novel Sphingopyxis species, for which the name Sphingopyxis flavimaris sp. nov. is proposed.

Bacterial Typing Techniques↗

PubMed2005

Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.

Two Gram-negative, non-spore-forming, slightly halophilic strains, SW-135T and SW-161T, which were isolated from a tidal flat of the Yellow Sea in Korea, were subjected to a polyphasic taxonomic study. The two isolates lacked bacteriochlorophyll a and contained ubiquinone-10 (Q-10) as the predominant respiratory lipoquinone and C(18 : 1)omega7c and C(17 : 1)omega6c as the major fatty acids. The DNA G+C contents of strains SW-135T and SW-161T were 62.2 and 64.5 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that the two strains fall within the radiation of the cluster comprising Erythrobacter species. Strains SW-135T and SW-161T exhibited a 16S rRNA gene sequence similarity value of 96.9 % and a mean DNA-DNA relatedness level of 12.3 %. Sequence similarities between strains SW-135T and SW-161T and the type strains of recognized Erythrobacter species ranged from 96.7 to 98.5 %. Levels of DNA-DNA relatedness were low enough to indicate that strains SW-135T and SW-161T represent members of two species separate from all recognized Erythrobacter species. On the basis of polyphasic taxonomic data, strains SW-135T (=KCTC 12228T=DSM 16221T) and SW-161T (=KCTC 12227T=DSM 16225T) were classified as two novel Erythrobacter species, for which the names Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov. are proposed, respectively.

Bacterial Typing Techniques↗

PubMed2005

Exiguobacterium aestuarii sp. nov. and Exiguobacterium marinum sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.

Three Gram-variable, rod-shaped bacterial strains, TF-16(T), TF-19 and TF-80(T), were isolated from a tidal flat of Daepo Beach (Yellow Sea) near Mokpo City, Korea, and their taxonomic positions were investigated by a polyphasic approach. These isolates grew optimally in the presence of 2 % NaCl and at 30 degrees C. Their peptidoglycan types were based on l-Lys-Gly. The predominant menaquinone detected in the three strains was MK-7. The three strains contained large amounts of the branched fatty acids iso-C(17 : 0), anteiso-C(13 : 0), iso-C(13 : 0) and iso-C(15 : 0). The DNA G+C contents of strains TF-16(T), TF-19 and TF-80(T) were 48.6, 48.4 and 48.0 mol%, respectively. The three strains formed a coherent cluster with Exiguobacterium species in a phylogenetic tree based on 16S rRNA gene sequences. They showed closest phylogenetic affiliation to Exiguobacterium aurantiacum, with 16S rRNA gene sequence similarity values of 98.1-98.3 %. The three strains exhibited 16S rRNA gene sequence similarity values of 94.0-94.6 % to the type strains of other Exiguobacterium species. Levels of DNA-DNA relatedness indicated that strains TF-16(T) and TF-19 and strain TF-80(T) are members of two species that are separate from E. aurantiacum. On the basis of phenotypic, phylogenetic and genetic data, strains TF-16(T) and TF-19 and strain TF-80(T) represent two novel species in the genus Exiguobacterium; the names Exiguobacterium aestuarii sp. nov. (type strain TF-16(T)=KCTC 19035(T)=DSM 16306(T); reference strain TF-19) and Exiguobacterium marinum sp. nov. (type strain TF-80(T)=KCTC 19036(T)=DSM 16307(T)) are proposed.

Bacillaceae↗

PubMed2005

Bacillus litoralis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.

A Gram-variable, motile, endospore-forming, slightly halophilic bacterial strain, designated SW-211(T), was isolated from a tidal flat of the Yellow Sea in Korea, and was characterized taxonomically by using a polyphasic approach. The organism grew optimally at 37 degrees C and in the presence of 2-3% NaCl. Comparative 16S rRNA gene sequence analysis showed that strain SW-211(T) forms a distinct phylogenetic lineage within the radiation of the cluster comprising Bacillus species. Strain SW-211(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. The predominant menaquinone was MK-7 and the major fatty acids were anteiso-C(15:0) (34.8%), iso-C(15:0) (15.6%), iso-C(16:0) (12.5%) and iso-C(14:0) (10.0%). The DNA G+C content was 35.2 mol%. Strain SW-211(T) exhibited levels of 16S rRNA gene sequence similarity of <96.2% with respect to the type strains of recognized Bacillus species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain SW-211(T) (=KCTC 3898(T)=DSM 16303(T)) was classified in the genus Bacillus as the type strain of a novel species, for which the name Bacillus litoralis sp. nov. is proposed.

Bacillus↗

PubMed2005

Nocardioides alkalitolerans sp. nov., isolated from an alkaline serpentinite soil in Korea.

Four Gram-positive, rod- or coccus-shaped bacterial strains, KSL-1(T), KSL-9, KSL-10 and KSL-12, were isolated from an alkaline serpentinite soil in Korea, and their taxonomic positions were investigated in a polyphasic study. The four strains exhibited no difference in their 16S rRNA gene sequences. Phylogenetic analyses based on 16S rRNA gene sequences showed that the four strains were phylogenetically affiliated to the genus Nocardioides. The four strains had cell-wall peptidoglycan based on ll-diaminopimelic acid as the diamino acid, indicating wall chemotype I. The predominant menaquinone detected in the four strains was MK-8(H(4)). The major fatty acid components were iso-C(16 : 0), 10-methyl-C(18 : 0), C(18 : 1)omega9c and C(17 : 1)omega6c. The DNA G+C contents were 72.4-73.6 mol%. The four strains exhibited 16S rRNA gene sequence similarity levels of 94.0-96.3 % to the type strains of Nocardioides species with validly published names. DNA-DNA relatedness levels between the four strains were 85-91 %. On the basis of phenotypic properties, phylogenetic distinctiveness and genotypic relatedness, strains KSL-1(T), KSL-9, KSL-10 and KSL-12 were classified in the genus Nocardioides as members of a novel species, Nocardioides alkalitolerans sp. nov. The type strain is strain KSL-1(T) (=KCTC 19037(T)=DSM 16699(T)).

Bacterial Typing Techniques↗

PubMed2005

Psychrobacter cibarius sp. nov., isolated from jeotgal, a traditional Korean fermented seafood.

Two novel Psychrobacter-like bacterial strains, JG-219(T) and JG-220, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of strains JG-219(T) and JG-220 were Gram-negative, non-motile coccobacilli. Growth of the two strains was observed at 4-32 degrees C. They grew optimally in the presence of 2-5 % (w/v) NaCl. Strains JG-219(T) and JG-220 contained C(18 : 1)omega9c and C(17 : 1)omega8c as the major fatty acids and Q-8 as the predominant ubiquinone. The DNA G+C contents of strains JG-219(T) and JG-220 were 43.5 and 43.0 mol%, respectively. The two strains showed no difference in their 16S rRNA gene sequences but exhibited minor differences in their phenotypic properties. Strains JG-219(T) and JG-220 exhibited levels of 16S rRNA gene sequence similarity of 95.2-98.7 % to the type strains of recognized Psychrobacter species. The mean level of DNA-DNA relatedness between strains JG-219(T) and JG-220 was 84.4 %. The two strains exhibited levels of DNA-DNA relatedness of 1.5-32.9 % to the type strains of eight phylogenetically related Psychrobacter species. On the basis of phenotypic data and phylogenetic and genetic distinctiveness, the two strains were classified as representing a novel species within the genus Psychrobacter, Psychrobacter cibarius sp. nov. The type strain is JG-219(T) (=KCTC 12256(T)=DSM 16327(T)).

Bacterial Typing Techniques↗

PubMed2005

Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea.

Four Gram-negative, rod-shaped bacterial strains, TF-26(T), TF-28, TF-42 and TF-53, were isolated from a tidal flat in the Yellow Sea, Korea, and their taxonomic positions were determined by a polyphasic characterization. The strains grew optimally in the presence of 2-3 % (w/v) NaCl and at 30-37 degrees C. The predominant menaquinone detected in the four strains was MK-6. These strains contained large amounts of fatty acids C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, iso-C(15 : 0), iso-C(16 : 0) 3-OH, C(15 : 0) and iso-C(17 : 0) 3-OH. The DNA G+C contents of the four strains were 32.3-32.8 mol%. Strains TF-26(T), TF-28, TF-42 and TF-53 showed 16S rRNA gene sequence similarity levels of 99.8-100 % and DNA-DNA relatedness levels of 82-87 %. The four strains exhibited 16S rRNA gene sequence similarity levels of 95.0-98.0 % to the type strains of the five current Tenacibaculum species, and DNA-DNA relatedness levels between the four strains and two phylogenetic relatives, Tenacibaculum mesophilum DSM 13764(T) and Tenacibaculum skagerrakense DSM 14836(T), were less than 21 %. On the basis of phenotypic, phylogenetic and genetic data, strains TF-26(T), TF-28, TF-42 and TF-53 were classified in the genus Tenacibaculum as members of a novel species, for which the name Tenacibaculum lutimaris sp. nov. (type strain, TF-26(T)=KCTC 12302(T)=DSM 16505(T)) is proposed.

Bacterial Typing Techniques↗

PubMed2005

Psychrobacter aquimaris sp. nov. and Psychrobacter namhaensis sp. nov., isolated from sea water of the South Sea in Korea.

Two Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strains, SW-210(T) and SW-242(T), were isolated from sea water of the South Sea in Korea, and were characterized taxonomically by means of a polyphasic approach. The two isolates grew optimally at 25-30 degrees C and in the presence of 2-3 % (w/v) NaCl. Strains SW-210(T) and SW-242(T) contained Q-8 as the predominant respiratory lipoquinone and C(18 : 1)omega9c as the major fatty acid. The DNA G+C contents of strains SW-210(T) and SW-242(T) were 43.2 and 45.3 mol%, respectively. The 16S rRNA gene sequences of strains SW-210(T) and SW-242(T) were 98.9 % similar, and the mean DNA-DNA relatedness value between them was 24 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains SW-210(T) and SW-242(T) form distinct phylogenetic lineages within the radiation of the cluster comprising Psychrobacter species, having 16S rRNA gene sequence similarity levels of 95.9-99.2 % with respect to the type strains of Psychrobacter species. The levels of DNA-DNA relatedness between the two isolates and the type strains of 15 phylogenetically related Psychrobacter species were well below 70 %. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains SW-210(T) and SW-242(T) were classified in the genus Psychrobacter as representing two distinct novel species, for which the names Psychrobacter aquimaris sp. nov. (type strain, SW-210(T) = KCTC 12254(T) = DSM 16329(T)) and Psychrobacter namhaensis sp. nov. (type strain, SW-242(T) = KCTC 12255(T) = DSM 16330(T)) are proposed, respectively.

Base Composition↗

PubMed2005

Algoriphagus yeomjeoni sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea.

Two Gram-negative, rod-shaped bacterial strains, MSS-160(T) and MSS-161, were isolated from a marine solar saltern in the Yellow Sea, Korea, and subjected to a polyphasic taxonomic study. The two strains grew optimally in the presence of 2 % (w/v) NaCl and at 25-30 degrees C. Strains MSS-160(T) and MSS-161 contained MK-7 as the predominant menaquinone and large amounts of fatty acids C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH and iso-C(15 : 0). The DNA G+C content of the two strains was 41 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the two strains were phylogenetically affiliated to the genus Algoriphagus. Strains MSS-160(T) and MSS-161 exhibited no difference in their 16S rRNA gene sequences and possessed a mean DNA-DNA relatedness level of 91 %; they exhibited 16S rRNA similarity levels of 96.6-99.3 % to the type strains of Algoriphagus species with validly published names. DNA-DNA relatedness levels between the two strains and the type strains of five Algoriphagus species were lower than 46 %. On the basis of phenotypic, phylogenetic and genetic data, strains MSS-160(T) and MSS-161 were classified in the genus Algoriphagus as members of a novel species, for which the name Algoriphagus yeomjeoni sp. nov. (type strain, MSS-160(T)=KCTC 12309(T)=JCM 12598(T)) is proposed.

Bacterial Typing Techniques↗

PubMed2005

Marinicola seohaensis gen. nov., sp. nov., isolated from sea water of the Yellow Sea, Korea.

A Gram-negative, non-flagellated, non-spore-forming and rod-shaped bacterial strain, SW-152(T), was isolated from sea water of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. Strain SW-152(T) grew optimally at 30 degrees C and in the presence of 2-3 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and iso-C(15 : 0) and iso-C(15 : 1) as the major fatty acids. Polar lipids detected in strain SW-152(T) were phosphatidylethanolamine, diphosphatidylglycerol and unidentified lipids. The DNA G+C content was 40.3 mol%. Phylogenetic trees based on 16S rRNA gene sequences exhibited that strain SW-152(T) forms a distinct evolutionary lineage within the Cytophaga-Flavobacterium-Bacteroides (CFB) group. Strain SW-152(T) exhibited low 16S rRNA similarity levels of less than 89.4 % to members belonging to the CFB group. Phenotypic properties of strain SW-152(T) differentiate it from phylogenetically related taxa. On the basis of phenotypic and phylogenetic data, strain SW-152(T) (=KCTC 12312(T)=JCM 12600(T)) was classified in a novel genus and species, Marinicola seohaensis gen. nov., sp. nov.

Bacterial Typing Techniques↗

PubMed2005

Sphingopyxis baekryungensis sp. nov., an orange-pigmented bacterium isolated from sea water of the Yellow Sea in Korea.

A Gram-negative, motile, slightly halophilic bacterial strain, SW-150(T), was isolated from sea water of the Yellow Sea, Korea, and was characterized by a polyphasic taxonomic approach. Strain SW-150(T) grew optimally at 25-30 degrees C and in the presence of 2 % (w/v) NaCl. The isolate could be distinguished from other Sphingopyxis species in producing an orange pigment. It contained ubiquinone-10 as the predominant respiratory lipoquinone and C(18 : 1)omega7c and C(17 : 1)omega6c as the major fatty acids. No 3-hydroxy fatty acids were detected. Major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 63 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SW-150(T) was phylogenetically affiliated to the genus Sphingopyxis of the family Sphingomonadaceae. Similarity values between the 16S rRNA gene sequences of strain SW-150(T) and the type strains of Sphingopyxis species ranged from 91.6 to 94.2 %, making it possible to categorize strain SW-150(T) as a species that is separate from previously described Sphingopyxis species. On the basis of phenotypic properties and phylogenetic distinctiveness, SW-150(T) (=KCTC 12231(T) = DSM 16222(T)) should be classified as the type strain of a novel Sphingopyxis species, for which the name Sphingopyxis baekryungensis sp. nov. is proposed.

Base Composition↗

PubMed2005

Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.

A Gram-negative, non-spore-forming, yellow-pigmented, slightly halophilic bacterial strain, SW-109(T), was isolated from a tidal flat of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. This isolate did not produce bacteriochlorophyll a and contained ubiquinone-10 as the predominant respiratory lipoquinone and C(18 : 1)omega7c as the major fatty acid. The DNA G + C content was 60.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SW-109(T) is phylogenetically affiliated to the genus Erythrobacter of the family Sphingomonadaceae. Strain SW-109(T) exhibited levels of 16S rRNA gene sequence similarity to the type strains of Erythrobacter species of 94.0-96.3 %, making it possible to categorize strain SW-109(T) as a species that is separate from previously recognized Erythrobacter species. On the basis of its phenotypic properties and phylogenetic distinctiveness, SW-109(T) (= KCTC 12311(T) = JCM 12599(T)) was classified as the type strain of a novel Erythrobacter species, for which the name Erythrobacter luteolus sp. nov. is proposed.

Bacterial Typing Techniques↗

PubMed2005

Aeromicrobium alkaliterrae sp. nov., isolated from an alkaline soil, and emended description of the genus Aeromicrobium.

A Gram-positive, aerobic, non-motile bacterial strain, KSL-107(T), was isolated from an alkaline soil from Korea and subjected to a polyphasic taxonomic study. Strain KSL-107(T) grew optimally at 25 degrees C and pH 7.0-7.5. Strain KSL-107(T) had a cell-wall peptidoglycan based on ll-2,6-diaminopimelic acid. It contained MK-9(H(4)) as the predominant menaquinone and C(16:0), C(16:0) 2-OH and 10-methyl C(18:0) as the major fatty acids. The DNA G+C content was 71.5 mol%. Comparative 16S rRNA gene sequence analyses showed that strain KSL-107(T) was phylogenetically affiliated to the genus Aeromicrobium of the family Nocardioidaceae. Similarity values between the 16S rRNA gene sequence of strain KSL-107(T) and those of type strains of Aeromicrobium species were in the range 97.9-98.2%. The isolate could be distinguished from other Aeromicrobium species by levels of DNA-DNA relatedness and differences in some phenotypic characteristics. On the basis of the data presented, it is suggested that strain KSL-107(T) (=KCTC 19073(T)=DSM 16824(T)) represents a novel Aeromicrobium species, for which the name Aeromicrobium alkaliterrae sp. nov. is proposed. An emended description of the genus Aeromicrobium is also proposed.

Actinomycetales↗

PubMed2005

Algoriphagus locisalis sp. nov., isolated from a marine solar saltern.

Two Gram-negative, rod-shaped, non-motile bacterial strains, MSS-170(T) and MSS-171, were isolated from sea water of a marine solar saltern of the Yellow Sea, Korea, and characterized by using a polyphasic taxonomic approach. The two isolates grew optimally at 30 degrees C and in the presence of 2% (w/v) NaCl. They were characterized chemotaxonomically as having MK-7 as the predominant menaquinone and major amounts of fatty acids iso-C(15:0) and C(16:1)omega7c and/or iso-C(15:0) 2-OH. The DNA G+C content of each of the two strains was 42 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the two strains fall within the evolutionary radiation enclosed by the genus Algoriphagus. Strains MSS-170(T) and MSS-171 had identical 16S rRNA gene sequences and exhibited a mean DNA-DNA relatedness level of 93%. The two strains exhibited 16S rRNA gene sequence similarity levels of 96.4-98.9% with respect to the type strains of recognized Algoriphagus species. DNA-DNA relatedness levels between the two strains and the type strains of six Algoriphagus species were less than 35%. On the basis of phenotypic data and phylogenetic and genetic distinctiveness, strains MSS-170(T) and MSS-171 were classified in the genus Algoriphagus as members of a novel species, for which the name Algoriphagus locisalis sp. nov. is proposed. The type strain is MSS-170(T) (=KCTC 12310(T)=JCM 12597(T)).

Bacterial Typing Techniques↗

PubMed2005

Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea.

A Gram-variable, motile, endospore-forming, slightly halophilic bacterial strain, DSW-10(T), was isolated from Dokdo, an island located at the edge of the East Sea, Korea, and was characterized taxonomically by using a polyphasic approach. This isolate grew optimally at 37 degrees C and in the presence of 4-5% NaCl. Strain DSW-10(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid, MK-7 as the predominant menaquinone, and anteiso-C(15:0), iso-C(15:0), anteiso-C(17:0) and iso-C(16:0) as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified phospholipids. The DNA G+C content was 36.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DSW-10(T) is phylogenetically affiliated to the genus Virgibacillus, and exhibited sequence similarity of 95.3-98.7% to the type strains of Virgibacillus species. DNA-DNA relatedness levels between strain DSW-10(T) and the type strains of some phylogenetically related Virgibacillus species were in the range 8.4-17.5%. On the basis of phenotypic properties and phylogenetic and genetic distinctiveness, strain DSW-10(T) (=KCTC 3933(T)=DSM 16826(T)) was classified as the type strain of a novel Virgibacillus species, for which the name Virgibacillus dokdonensis sp. nov. is proposed.

Bacillaceae↗

PubMed2005

Nocardioides kribbensis sp. nov., isolated from an alkaline soil.

Three Gram-positive, rod- or coccoid-shaped bacterial strains, KSL-2(T), KSL-5 and KSL-6, were isolated from an alkaline soil in Korea and subjected to a polyphasic taxonomical analysis. These isolates grew optimally at pH 9.0 and 30 degrees C. They were characterized chemotaxonomically as having cell wall peptidoglycan based on ll-2,6-diaminopimelic acid, MK-8(H(4)) as the predominant menaquinone and iso-C(16:0) as the major fatty acid. The DNA G+C content of the isolates was 73-74 mol%. Strains KSL-2(T), KSL-5 and KSL-6 were identical in their 16S rRNA gene sequences and exhibited DNA-DNA relatedness values of 88-93%. Phylogenetic trees based on 16S rRNA gene sequences showed that the three isolates fell within the evolutionary radiation encompassed by the genus Nocardioides. Levels of 16S rRNA gene sequence similarity between the three strains and the type strains of Nocardioides species ranged from 93.6% (with Nocardioides albus) to 97.2% (with Nocardioides aquiterrae). DNA-DNA relatedness levels between the three isolates and N. aquiterrae CJ-14(T) were 8-15%. On the basis of phenotypic, phylogenetic and genetic data, strains KSL-2(T), KSL-5 and KSL-6 were classified in the genus Nocardioides as members of a novel species for which the name Nocardioides kribbensis sp. nov. is proposed, with KSL-2(T) (=KCTC 19038(T)=DSM 16314(T)) as the type strain.

Actinomycetales↗

PubMed2005

Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea.

A Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strain, SW-238(T), was isolated from sea water collected from the South Sea in Korea, and subjected to a polyphasic taxonomic study. The organism grew optimally at 25-30 degrees C and in the presence of 2-3% (w/v) NaCl. Strain SW-238(T) was characterized chemotaxonomically as containing ubiquinone (Q-8) as the predominant respiratory lipoquinone and C(18:1)omega9c as the major fatty acid. Its DNA G+C content was 47.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SW-238(T) fell within the radiation of the cluster comprising Psychrobacter species. The 16S rRNA gene sequence of strain SW-238(T) had similarity levels of 94.8-97.9% to sequences of the type strains of recognized Psychrobacter species. Levels of DNA-DNA relatedness between strain SW-238(T) and the type strains of 10 phylogenetically related Psychrobacter species were below 70%. On the basis of phenotypic and phylogenetic data and genetic distinctiveness, strain SW-238(T) (=KCTC 12313(T)=JCM 12601(T)) was placed in the genus Psychrobacter as the type strain of a novel species, for which the name Psychrobacter celer sp. nov. is proposed.

Bacterial Typing Techniques↗