Search PubMed⌕ Search

Biomedical subjects

T van Slochteren-Draaisma

Publications and source records attributed to T van Slochteren-Draaisma.

5 recordsLinked to original sources
PubMed1981

Induction of the shaking phenomenon by pretreatment of spermatozoa with sera containing antispermatozoal antibodies.

Sera containing sperm-agglutinating and/or complement-dependent sperm-immobilizing activity were tested for their ability to induce a shaking phenomenon in the sperm-cervical mucus contact (SCMC) test. Donor spermatozoa were treated with the sera in a one-step incubation and washing procedure. The percentage of motile pretreated spermatozoa showing the shaking phenomenon (S%) was determined in the SCMC test. In addition, the immunoglobulin class of antibodies present on the pretreated spermatozoa was determined in mixed antiglobulin reaction tests for IgG, IgM, and IgA. An S% of at least 80 was always observed with spermatozoa pretreated in sera with a sperm agglutination titer of at least 32, provided that IgG was detected on more than 90% of the motile spermatozoa. The high S% was also obtained with spermatozoa pretreated in purified IgG from sera containing antispermatozoal antibody activity. It was concluded that the reduced cervical mucus penetration capacity of spermatozoa pretreated with sera containing antispermatozoal IgG could be ascribed, at least partially, to the occurrence of a shaking phenomenon.

Antibodies↗
PubMed1980

Immunoglobulin class of antispermatozoal antibodies from infertile men and inhibition of in vitro sperm penetration into cervical mucus.

The presence of IgG and IgA on motile spermatozoa from normal semen donors and men from infertile couples was studied with mixed antiglobulin reaction (MAR) tests. The percentage of motile spermatozoa with IgG (IgG MAR %) was found to be related to the circulating antispermatozoal IgG. No direct relation could be detected between the IgG MAR % and the sperm agglutinating activity in seminal plasma (SP) or the percentage of motile spermatozoa showing the shaking phenomenon (S%) in the sperm cervical mucus contact (SCMC) test. The percentage of motile spermatozoa with IgA (IgA MAR %) showed no direct relation to the sperm agglutinating activity in serum and SP, but was roughly proportional with the S%. It was discerned that the shaking phenomenon in the SCMC test was probably due to presence of IgA on the motile spermatozoa. Previously it had been demonstrated that also the sperm agglutinating activity in SP is caused by IgA that is probably locally produced in the male genital tract. In conclusion it was thought that the reduced ability of penetration into cervical mucus by spermatozoa from infertile men is caused by locally produced IgA.

Cervix Mucus↗
PubMed1978

A simple method of screening for antisperm antibodies in the human male. Detection of spermatozoal surface IgG with the direct mixed antiglobulin reaction carried out on untreated fresh human semen.

A simple and rapid test for the detection of antisperm antibodies of the IgG class on freely swimming spermatozoa in fresh human semen is described. The test is based on the formation of motile mixed agglutinates between erythrocytes sensitized with incomplete anti-Rh-antibodies and freely swimming spermatozoa with surface antisperm antibodies, after mixing both cell types together with anti-IgG antiserum. Agglutination of the red blood cells serves as an internal control. The test can be applied on ejaculates with spermatozoa concentrations down to one million per ml, provided the motility is sufficient. The percentage of motile spermatozoa found to be coated with antisperm antibodies of the IgG class, and the extent of the coating, proved to be correlated with the agglutination titer of circulating antisperm antibodies and with the inhibition of sperm penetration into cervical mucus. The test can be used as a screening for the presence of antisperm autoantibodies in serum and semen.

Agglutination↗
PubMed1978

The "unexplained" poor postcoital test.

In 30 or 32 infertile couples with an unexplained negative or bad in vivo and in vitro sperm penetration test, we obtained a strongly positive Sperm Cervical Mucus Contact test (SCMC-test) and demonstrated the presence of antisperm antibodies in the male or female partner. In these 30 couples 25 of the male partners had a sperm-agglutination titre of at least 32 in the serum and at least 4 in the seminal plasma. In the five remaining couples the female partner showed a minimum sperm-agglutination titre of 16 in the serum and a cervical mucus titre of at least 128. In 48 couples with a fair or good sperm penetration in cervical mucus, in vivo and in vitro, we never found a strongly positive SCMC-test. In 43 of these couples the SCMC-test was negative. Only one man in the latter group had sperm-agglutinating activity in the semen. In a group 32 couples, with a negative SCMC-test, there was no or only weak sperm-agglutinating activity in the cervical mucus, although 2 women had moderate sperm-agglutinating activity in the blood serum. Based on these data we conclude that the so called "unexplained" poor postcoital test is almost always due to the presence of antisperm antibodies in the semen or in the cervical mucus. We consider the SCMC-test not only to be a simple and reliable technique for detecting the presence of these antisperm antibodies, but also a method of demonstrating the mechanism by which antisperm antibodies decrease the chance of conception.

Antibodies↗