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Biomedical subjects

T Zalewski

Publications and source records attributed to T Zalewski.

At least 19 recordsLinked to original sources
PubMed1998

Dioctahedral smectite neutralization activity of Clostridium difficile and Bacteroides fragilis toxins in vitro.

The neutralization activity of dioctahedral smectite for ten toxigenic Clostridium difficile and eight enterotoxigenic Bacteroides fragilis strains was studied using McCoy and HT 29/C1 cell lines, respectively. Minimalization of the cytopathic effect of C. difficile toxin B on McCoy cell lines by dioctahedral smectite dissolved in PBS was observed. After incubation with dioctahedral smectite the toxic effects of B. fragilis enterotoxins on HT/29C1 (human colon adenocarcinoma cell line) were eliminated. Best neutralization of B. fragilis toxin was achieved using dioctahedral smectite dissolved in BHI.

Animals↗
PubMed1989

[Activity and molecular form of intestinal gamma-glutamyltransferase (GGT) EC 2.3.2.2. in celiac disease].

It has been shown that GGT activity in the duodenal biopsy homogenates of the children with coeliac disease (n-10) in remission (1 to 3 years of gluten-free diet) is lower than in those with other gastrointestinal tract diseases (n-6). In children with coeliac disease after gluten challenge (1 g of gluten) kg BW for 3 to 6 months) the GGT activity decreased fourfold (n-10). After a few months of gluten challenge there was in coeliac children (n-5) a marked predominance of GGT without sialic acid (the asialic GGT). Similarly there was a prevalence of this form (n-5) in the gut tissue of 3 month old human fetus. In the homogenates of the duodenal bioptates of the children with other gastrointestinal tract diseases (n-6) there was a predominance of the sialic form of the GGT. In the gut tissue of children older than 3 years (n-6) and adults who died of reasons other than gastrointestinal a marked predominance of the sialic form of GGT was found. It has been suggested that presence of asialic form of GGT in coeliac disease is connected with the lectin-like activity of gluten. The process of sialization or desialization takes place within or outside enterocytes. It changes the gut permeability and causes a secondary reaction to the penetrating allergens.

Celiac Disease↗
PubMed1989

[The effect of 6-month provocation with gluten on the height and weight of children with celiac disease].

Gluten challenge was performed in 22 coeliac children in the period of histological remission. Gluten in dose 0.5 g/kg was administered for 6 months. Before the Provocation Test over 60% of children have had body weight over 50 c for normal children in Warsaw. Standardized mean body weight (+ 0.13 +/- 1.1) and height (+ 0.04 +/- 1.05) of the investigated coeliac children did not differ significantly from the values of normal Warsaw population (p greater than 0.005). A 6 month provocation with gluten leading to the histological relapse did not affect body weight and height of tested children; over 50% have had body weight and height over 50 c. Mean standardized body weight (- 0.002 +/- 1.0) and height height (-0.07 +/- 0.91) did not differ significantly from the normal values (p greater than 0.05) and corresponding values before the provocation (p greater than 0.05). It seems that Provocation Test with gluten carried out in the above way does not inhibit physical development of children with coeliac disease.

Body Height↗
PubMed1989

[Evaluation of the effect of gluten-free diet on nutritional status of children with florid celiac disease].

A dynamics of Nutritional Status of 55 children with recently diagnosed coeliac disease was assessed during the treatment with gluten free diet. No deterioration of the nutritional status was seen in 34.1% of the investigated children (time 0) whereas different degree of nutritional deficiency was noted in the remaining 75.9% of children including 10% of children with severe deficiency. After a 2-year therapy with gluten-free diet a deteriorated nutritional status was only found in 8.6% of children. In 27 children with gluten free diet for nearly 3 years, with maintenance of histological remission, the percentage of children with nutritional deficiency was 7.2%. The fact of normal Nutritional Status in more than 30% of the investigated coeliac children at the time of the initial diagnosis seems to be an effect of early and proper initial diagnosis.

Celiac Disease↗