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Biomedical subjects

T Yumoto

Publications and source records attributed to T Yumoto.

At least 37 records · Page 2Linked to original sources
PubMed1988

Intraosseous epidermal cyst of the sacrum. A case report.

An unusual case of intraosseous epidermal cyst is reported. The patient, a 45-year-old Japanese female, had suffered from lumbago and dysuria for about 15 years. X-ray examinations and CT scan revealed an expanded osteolytic tumor without marginal sclerotic change within the sacrum, which anteriorly invaded the surrounding soft tissues at the S2/3 level. At this time, chordoma was suspected, but epidermal cyst with foreign body granuloma was finally diagnosed from biopsy and surgical specimens.

Adult↗
PubMed1988

Osteosarcoma. Ultrastructural and immunohistochemical studies on alkaline phosphatase-positive tumor cells constituting a variety of histologic types.

The osteosarcomas were subclassified into osteoblastic, fibroblastic, chondroblastic and telangiectatic types and examined by electron microscopy. Their immunohistochemical reactions were also studied. In an overall survey of the above types, fibroblast-like cells revealed poorly developed cytoplasmic organelles with rather short, branching rough endoplasmic reticulum, mixed with osteoblast-like cells that were hardly distinguishable from the former. They appeared to be an early stage of an osteoblastic cell lineage from the distribution and development of their cell organelles and highly positive vimentin activity. The tumor cells in malignant cartilage varied in appearance from chondroblast-like to osteoblast-like cells. All types of tumor cells expressed alkaline phosphatase activity to a significant degree. Immunohistochemical staining showed a mixture of procollagen type I-positive cells among the cells positive for both procollagen type II and S-100 protein in the malignant cartilage. Irrespective of any ultrastructural differences between these various tumor cell types, they all revealed a significant degree of ALPase activity unlike other types of bone tumors, suggesting that the tumor cells which constitute the various types of osteosarcoma are derived from a common precursor cell.

Adolescent↗
PubMed1987

Alkaline phosphatase-positive reticular cells of chicken bone marrow--in vivo and in vitro studies.

We examined alkaline phosphatase (ALPase)-positive reticular cells from chicken bone marrow in vitro in relation to other varieties of adherent cells. ALPase activity was found in both reticular and adipose cells which formed epithelioid cell colonies and were negative for fibronectin. We observed transition between two cell types. ALPase-negative macrophages as small round cells in culture revealed positive fibronectin and transformed into ALPase-negative spindle cells in long-term culture. Thus, we suggest two cell lineages, each with distinct cell characteristics.

Alkaline Phosphatase↗
PubMed1987

Experimental tumors of myxoid malignant fibrous histiocytoma and hyaluronic acid production.

Using B-10 tumor cells originated from mouse peritoneal macrophages transformed by simian virus 40, we succeeded in producing tumors in an ascitic form similar to human myxoid malignant fibrous histiocytoma. The tumor cells possessed Fc and C3 receptors, immunophagocytic activity, and lysosomal enzymes. They showed pseudopodic extensions of the cytoplasm containing lysosomes. Therefore, they maintained the functional and morphological characteristics of macrophages. On cellulose acetate electrophoresis with or without enzymatic degradation, the ascitic fluid contained a single component of glycosaminoglycans; hyaluronic acid. Electron microscopy utilizing dialyzed iron demonstrated electron-dense reaction products on the cell surfaces. Thus, the histiocytic origin of malignant fibrous histiocytoma was suggested and possibility was expressed, concerning the histogenesis of myxoid malignant fibrous histiocytoma, that the transformed tumor cells could synthesize hyaluronic acid on the cell surface and release it into the stroma.

Animals↗
PubMed1987

Osteoclast-like giant cell tumor of the liver.

A 66-year-old male with osteoclast-like giant cell tumor of the liver that arose in the non-cirrhotic liver is presented. The liver tests were almost normal, and plasma levels of alpha-fetoprotein and carcinoembryonic antigen were within normal limits. The findings of liver scan by 99mTc phytate, celiac angiography, and CT scans are described for the first time for this rare neoplasm, showing a large, unresectable liver tumor. Histologically, the tumor mainly consisted of osteoclast-like giant cells and mononuclear cells, which were focally arranged in a vaguely trabecular pattern and sarcomatous pattern. By an electromicroscopic study, however, no definitive evidence was obtained whether it arose from epithelial cells or nonepithelial cells. Various clinicopathological features were described and compared with previously reported cases including two cases arising in the liver.

Aged↗
PubMed1987

High alkaline phosphatase activity of telangiectatic osteosarcoma (TOS) and its diagnostic significance.

Telangiectatic osteosarcoma (TOS) which occurred in the metaphysis of the right femoral bone in a 13-year-old female was reported. It showed osteolytic and cystic lesion without sclerotic change on roentgenogram and consisted histologically of various sized blood-filled spaces lined by layers of round to oval tumor cells in the thin fibrous septa. In some solid areas, a proliferation of atypical tumor cells with large prominent nucleoli was evident, embedded in the lace-like osteoid tissue. Mitotic cells were easily encountered. A large population of tumor cells revealed high alkaline phosphatase activity as well as 5'-nucleotidase activity, indicative of osteogenic cell origin. Ultrastructurally, they showed osteogenic characteristics of well-developed rough endoplasmic reticula, cytoplasmic microfibrils, and dense bodies, but not for those of endothelial cells. In this report, we suggest that alkaline phosphatase activity in biopsy and surgical specimens is useful for distinguishing TOS from other osteolytic bone tumors, with regard to its ontogenic discussion.

Adolescent↗
PubMed1983

The identification and characterization of antimacrophage serum (AMS) and its application to murine fibrous histiocytoma.

The preparation of antimacrophage serum (AMS) and its application to murine fibrous histiocytoma are reported. AMS, prepared by inoculating peritoneal macrophages from mice into rabbits, was purified by repeated absorption with kidney homogenate, red blood cells, thymus cells, non-adherent spleen cells and finally, L-cells. The molecular weight was determined as 170,000 daltons using SDS-polyacrylamide gel electrophoresis. AMS diluted to 1:32 reacted with 61.4% of peritoneal macrophages, but not red blood cells, lymphocytes, granulocytes and fibroblasts, as judged by membrane immunofluorescence. 57.1% of monoclonal B-10 macrophages and 75.6% of 28-12 macrophages transformed with SV40 reacted with AMS. B-10 and 28-12 cell tumors simulating human malignant fibrous histiocytoma gave a distinctive diffuse pattern of immunofluorescence with AMS which affected both round- and spindle-shaped cells, although positive fluorescence of spindle-shaped cells was somewhat weaker than that of round-shaped cells. However, tumor tissues from a murine fibrosarcoma showed no reaction with AMS. Based on these findings, AMS may be useful in determining the histogenesis of some soft tissue tumors of doubtful origin.

Animals↗
PubMed1982

Giant cell tumor bone. Enzyme histochemical, biochemical and tissue culture studies.

Three giant cell tumors of bone (2 benign and 1 malignant) were examined enzyme-histochemically, and a tissue culture study of the malignant case was performed. Multinucleated giant cells and mononuclear round cells had similar activities of ACPase and non-specific esterase with a diffuse strong reaction. ATPase and 5'-nucleotidase reactions were strongly positive in the cytoplasm of multinucleated giant cells, and were seen not only in the cytoplasm but also on the cell membrane of round cells. The proliferating spindle cells in the malignant case were faintly positive for ACPase and non-specific esterase and were less positive for ATPase and 5'-nucleotidase on the cell membrane. The multinucleated giant cells and mononuclear round cells resembled histiocytes in the activities of 4 hydrolytic enzymes, and the multinucleated giant cells had enzyme activities similar to those of osteoclasts from new-born rat skull. The malignant giant cell tumor and cells in its tissue culture showed ALPase activity preferentially on the cell membrane of the spindle cells, and rarely on round cells or multinucleated giant cells. ALPase was resistant to heat treatment and was found to be the type IV isoenzyme by diffusion electrophoresis. The origin of the giant cell tumor of bone and the significance of the ALPase activity are discussed.

5'-Nucleotidase↗
PubMed1982

Dermatofibrosarcoma protuberans and its tissue culture study -- ultrastructural, enzyme histochemical and immunological study.

Dermatofibrosarcoma protuberans arizing in the thigh of a 23-year-old female is reported with an investigation of tumor cell character by electron microscopy, enzyme histochemistry, immunological and in vitro tissue culture techniques. The tumor tissue showed histologically a peculiar storiform arrangement of plump spindle cells with dense proliferation. Electron microscope revealed histiocytic cell structures of some tumor cells: lysosomes, phagosomes, and sparse rough endoplasmic reticula. They revealed diffuse positive reactions to acid phosphatase (Ac Pase) and non-specific esterase. Short-time culture of the tumor tissue indicated rather round and polygonal cells admixed with spindle cells, positive to Ac Pase and non-specific esterase with diffuse reaction. Most of the round cells possessed Fc and C3 receptors on their surface and phagocytic activity. Dermatofibrosarcoma protuberans is probably a histiocytic tumor and structural characteristics of schwann cells were not recognized.

Adult↗
PubMed1982

Postirradiation sarcoma (malignant fibrous histiocytoma) in the inguinal region.

A female patient who died of apparent postirradiation sarcoma at the age of 39 was reported. Following a traffic accident, she developed squamous cell carcinoma at the injured right heel 20 years later, and also metastasis to both inguinal and popliteal lymph nodes. During the period from September 1966 to May 1968, she had received Co60 irradiation to the right inguinal and para-aortic areas, 9,600 and 6,600 Roentgen, respectively. Ten years later, she manifested a huge tumor in the inguinal region which extended further to the retroperitoneum; light and electron microscopic examinations proved to be a malignant fibrous histiocytoma. She died of massive pulmonary metastasis two years later. Histological differentiation of the tumor and relationship between irradiation and sarcoma induction were briefly discussed, and the necessity of reevalution of soft part sarcomas was emphasized.

Abdominal Neoplasms↗
PubMed1982

A tumor composed of myofibroblasts: an ultrastructural study.

A subcutaneous tumor of the right temporal region of head was removed from an 8-year-old boy. This tumor was studied by light and electron microscopy. He was found to have had a retroperitoneal inflammatory fibrous histiocytoma which had been excised seven years ago. The subcutaneous tumor histologically consisted of spindle cells in fascicles, which did not arrange in a storiform or herring-bone pattern. Electron microscopically, this tumor was chiefly composed od myofibroblasts. electron microscopy is essential to determine the cell types of a tumor and we have classified this tumor as a tumor of myofibroblasts. The relationship between the retroperitoneal tumor and the head tumor was discussed.

Brain Neoplasms↗
PubMed1982

Fibrous histiocytoma of the nasal cavity and maxillary sinus.

Two cases of fibrous histiocytoma were presented; Case 1: a 46-year-old man with a tumor filling the left nasal cavity and maxillary sinus, and Case 2: an 80-year-old man with a tumor of the right maxillary sinus destroying its surrounding wall. Histologically, the former was dominated by storiform pattern and onion-like structures, and the latter by osteoclast-like multinucleated giant cells. After radical surgery, Case 1 was free of recurrence for more than one year, and Case 2 showed recurrence although still maintaining fairly good health. A total of 29 cases hitherto reported arising in the nasal, paranasal and nasopharyngeal regions were reviewed, pitfall of differential diagnoses was commented especially in our two cases, criteria of malignancy were discussed, and the necessity of repeated samplings was emphasized because of paucity and unfamiliarity of fibrous histiocytoma arising, in particular, from the upper respiratory tract.

Aged↗
PubMed1982

Enzyme histochemical study on bone tumors.

A total of 19 cases with bone tumors, including six osteosarcomas. three giant cell tumors of bone, one malignant fibrous histiocytoma, four nonossifying fibromas, four chondromas and one chondrosarcoma, were examined as to enzyme histochemistry; the enzymes consisted of alkaline phosphatase (ALPase), acid phosphatase (ACPase), nonspecific esterase (NSE), adenosine triphosphatase (ATPase), 5'-nucleotidase (5'-Nucl) and beta-glucuronidase (beta-Gl). Osteosarcoma was strongly positive for ALPase followed by 5'-Nucl. Giant cell tumor, malignant fibrous histiocytoma and nonossifying fibroma showed enzyme histochemistry similar to each other: multinucleated giant cells and round cells in these tumors were strongly positive for ACPase, NSE, ATPase and 5'-Nucl simulating osteoclasts and histiocytes, whereas spindle cells were positive for ATPase and 5'-Nucl in their cytoplasm and weakly positive for ACPase. Chondroma and chondrosarcoma were focally positive for ACPase and NSE; the ACPase was sensitive to tartaric acid treatment. These observations showed that ALPase activity is very characteristic to osteosarcoma, and is useful for its diagnosis. From enzyme histochemistry, giant cell tumor, malignant fibrous histiocytoma and nonossifying fibroma can be regarded as a histiocyte-derived tumor of bone in contrast to osteosarcoma and cartilaginous tumors.

Acid Phosphatase↗
PubMed1981

Cytochemical markers of murine leukemias and lymphomas.

Seven transplantable leukemias and lymphomas which occurred in NZB mice were characterized immunologically and cytologically as B-cell type of L66-, 2709-, 2769- find S77-lines, and T-cell type of TH17-, TH90- and S29-lines. These were also studied with enzyme histochemistry in tissue sections. All B-cell tumors revealed strong activities of ATPase and 5'-nucleotidase and one expressed A1Pase activity on the cell surface. Two thymic lymphomas (TH17- and TH90-lines) and one T-cell leukemia (S29-line) showed negative reactions of ATPase and 5'-nucleotidase but positive activities of AcPase and non-specific esterase localized in their cytoplasms. A1Pase activity was expressed in TH90-lymphoma but not in TH17- and S29-lymphomas. A1Pase recognized in L66- and TH90-lymphomas showed similar reaction to those of Nagao's isoenzyme. The splenic follicles of normal NZB mice reacted to ATPase and 5'-nucleotidase in B-cell area (primary follicles) and to AcPase and non-specific esterase in T-cell area (PALS) with the localized reaction. Data suggest that four enzymes of ATPase, 5'-nucleotidase, AcPase and non-specific esterase are useful for the differentiation of B- and T-cells and their malignant counterparts.

Acid Phosphatase↗