Biomedical subjects

T Yazawa

Publications and source records attributed to T Yazawa.

92 records · Page 6Linked to original sources

PubMedDate not supplied

Combined effects of TNP-470 and taxol in human non-small cell lung cancer cell lines.

The effects of TNP-470, one of the promising antiangiogenic agents and taxol when combined were evaluated in two human cultured cell lines derived from non-small cell lung cancer(NSCLC). Cytotoxicity was determined using a tetrazolium salt(MTT) assay. The inhibitory effects of TNP-470 on cell proliferation were dose related and the 50% inhibitory concentration on these cell lines were 50.0 to 62.5 microM. Evaluation of drug interactions with isobologram and the combination index values showed that sequential exposure to taxol followed by TNP-470 produced synergistic effects in these cell lines tested. Our findings suggest that such an angiocytotoxic chemotherapy might be promising for the treatment of NSCLC.

Antineoplastic Combined Chemotherapy Protocols↗

PubMedDate not supplied

Lung cancer cell line producing cytokeratin 19 fragment and sialyl Lewis X-i antigen.

A new human lung large cell carcinoma cell line producing cytokeratin 19 fragment(CYFRA) and sialyl Lewis X-i antigen(SLX), designated TKB-13, was established from a patent in our laboratory. The cell line grew in a monolayer and had a polygonal epitheloid appearance in phase-contrast microscopy. The doubling time of TKB-13 cells was approximately 32 hours in Dulbecco's minimal essential medium with 5% fetal bovine serum. TKB-13 cells could be transplanted to nude mice and the histological characteristics were the same as those of resected tissue from the primary site. Positive reactions for CYFRA and for SLX were seen within the cytoplasm of TKB-13 cells by immunocytochemical staining. Both CYFRA and SLX were detected in the culture supernatant. Our results suggested that TKB-13 cell line is available for experimental studies on the biological behavior of non-small cell lung cancer.

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