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T Yasuda

Publications and source records attributed to T Yasuda.

At least 1,045 records · Page 58Linked to original sources
PubMedDate not supplied

Population studies of human deoxyribonuclease I polymorphism.

We have improved the resolution of the conventional method for phenotyping deoxyribonuclease I (DNase I), which makes use of isoelectric focusing, by the addition of amphoteric separators. The distribution of DNase I phenotypes was extensively examined using this improved method in 1,212 unrelated individuals from a Japanese population. In order to investigate a possible difference in phenotype distribution between different populations, DNA samples from Germans and African Americans were genotyped using the polymerase chain reaction. The DNASE1*2 allele in the German population was found to be predominant among the four alleles of DNase I, in contrast to the Japanese population. These results are the first to demonstrate a wide distribution of DNase I polymorphism in the Japanese population as well as in two other populations.

Asian People↗
PubMedDate not supplied

The effects of recombinant human erythropoietin on autologous blood donation in rheumatoid arthritis patients with anaemia.

OBJECTIVES: The effect of recombinant human erythropoietin (rHuEPO) treatment on autologous blood donation was evaluated in anaemic patients with rheumatoid arthritis (RA) undergoing total joint replacement surgery. METHODS: A total of 56 total knee or hip joint replacement operations were performed in the knee or hip joint in 36 anaemic RA patients (hemoglobin (Hb) concentration < 11.0 g/dl]. All of the patients received intravenous rHuEPO at a dose of 100-200 units/kg body weight three times a week for 3 weeks. An autologous blood donation of 800-1200 g was the goal for each patient. A refractory case was defined as a patient whose Hb level did not increase to 10.0 g/dl after 3 weeks of treatment with rHuEPO. The objective signs of arthritis were assessed by the Lansbury activity index (AI). During the treatment period, the patients underwent weekly hematological analyses, including routine hematology, serum iron, serum ferritin, C-reactive protein (CRP), and serum erythropoietin levels. RESULTS: The response to rHuEPO treatment was determined, and blood donation was possible in 47 of 56 joint replacements. In the other 9 operations, donation was not possible due to a poor response to rHuEPO. The mean Hb level before treatment in the refractory group (8.3 g/dl) was significantly lower than that in the responsive group (10.4 g/dl, p = 0.0002). During the treatment period, the mean erythropoietin level was above the normal limit in the refractory group. The mean AI for the refractory group tended to be lower than that in the responsive group. The mean pre-treatment CRP (6.4 mg/dl) and erythrocyte sedimentation rate (ESR) (87.1 mm/h) levels in the refractory group were significantly higher than those in the responsive group (CRP: 3.2 mg/dl, p = 0.008, ESR: 52.6 mm/h, p = 0.01). CONCLUSIONS: The control of disease activity prior to rHuEPO treatment is considered to a prerequisite for autologous blood donation. In addition, severe anaemia (Hb concentration < 8.0 g/dl) appears to be another risk factor for refractoriness to rHuEPO treatment with the present protocol. A higher rHuEPO dose (> 200 units/kg/3 times a week for three weeks) was considered to be necessary in the refractory group.

Adult↗
PubMedDate not supplied

Pancreas-sparing duodenectomy: classification, indication and procedures.

BACKGROUND/AIMS: Recent advances in the surgical anatomy of the pancreatoduodenal region have permitted duodenum-preserving pancreatic head resection. However, pancreas-sparing duodenectomy (PSD) has not been systematically studied and various types of such procedures have been reported under the designation of PSD. METHODOLOGY: PSD was performed in 6 patients with extensive duodenal lesions including trauma, mucosa-associated lymphoid tissue (MALT) lymphoma, corrosive necrosis, bleeding, leiomyosarcoma and congenital stenosis. Three patients had the whole papilla Vateri and half of the duodenum preserved and anastomosed to the jejunum. One patient had the duodenal button including the papilla of Vater transplanted to the jejunum. Another patient had the intraduodenal portion of the major papilla excised and the terminal portion of the bile and pancreatic ducts anastomosed to the jejunum. RESULTS: Two patients with moribund conditions died of the underlying disorders 2 weeks and 3 months after surgery, respectively, but without leakage or other surgery-associated complications. The other 4 patients survived the surgery without anastomotic insufficiency. Three survivors, who had complete preservation of the major and minor papillae along with the half portion of the duodenum, had normal morphology and function of the biliopancreatic system post-operatively. The fourth survivor, that with excision of the intrapancreatic portion of the major papilla, had regurgitation of contrast material into the bile and pancreatic ducts 2 months after surgery on active insufflation of the intestinal lumen, but remained asymptomatic. No abnormality in liver and pancreatic function was detected as of 7 months post-operatively. CONCLUSIONS: PSD appears to be applicable in the clinical setting, although classification of the procedure seems mandatory in consideration of indications, techniques, and long-term consequences of biliopancreatic function.

Adult↗
PubMedDate not supplied

Externalization of calpain (calcium-dependent neutral cysteine proteinase) in human arthritic cartilage.

OBJECTIVE: Calcium-dependent neutral cysteine proteinase (calpain) was originally referred to as an intracellular enzyme. However, recently it has come to be considered as an extracellular matrix proteinase, as well having a degrading effect on cartilage proteoglycan. In the present study we sought to determine whether human articular cartilage chondrocytes themselves have the capability to produce and secrete this interesting proteinase. METHODS: Human articular cartilage tissue cultures from osteoarthritic (11 specimens from 7 patients) and rheumatoid arthritic (3 specimens from 2 patients) knee joints were established, and the m-calpain released into the culture medium was concentrated and detected by immunoelectrophoretic blotting. The presence of m-calpain in the arthritic cartilage was also examined by immunohistochemistry before and after culturing. RESULTS: M-calpain was detectable in all of the cartilage tissue culture supernatants (conditioned medium) by western blotting. Positive intracellular immunostaining of m-calpain in chondrocytes was observed in all samples. Furthermore, m-calpain was found to be present in the matrix and on the articular surface of the cartilage in half of the specimens. CONCLUSION: The findings of our experiment suggest that cartilage chondrocytes may actively take part in m-calpain production and that they may have the capacity to release it into the extracellular matrices.

Aged↗
PubMedDate not supplied

[Report of a patient with CADASIL having a novel missense mutation of the Notch 3 gene--association with alopecia and lumbar herniated disk].

We report a 52-year-old man with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) presenting dementia, alopecia and lumbar herniated disk. He had an episode of stroke and migraine-like headache lasting for 5 minutes. A lot of members had cerebral infarction in this family. Brain magnetic resonance imaging demonstrated, on T2-weighted images, numerous hyperintense lesions suggestive of small infarcts in the basal ganglia and diffuse hyperintense lesions in the cerebral white matter. The clinical symptoms, the family history and the MRI findings suggested the diagnosis of CADASIL. However, the patient also showed alopecia and lumbar herniated disk, both are characteristic features of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The DNA analysis of the Notch 3 gene identified a novel missense mutation Cys174Phe in this patient. Our case report indicated the importance of the DNA analysis for the diagnosis of CADASIL.

Alopecia↗
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Multiple neurilemmoma of the cauda equina. A case report.

A 49-year-old male, with a relatively uncommon multiple neurilemmoma, exhibited symptoms of cauda equina compression. Following a diagnosis of multiple tumor of the cauda equina and extradural cyst, based on myelography and CTM, the tumors at L1-L2, the left S1 nerve root, and left hip were resected. Three years after the operation, the patient is asymptomatic apart from slight numbness in the right hip and there is no evidence of tumor recurrence. The rarity of such a case prompted this report.

Cauda Equina↗
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Absorption of topical disodium cromoglycate and its preservatives by soft contact lenses.

Disodium cromoglycate (2%) (DSCG) was administered four times daily for one month to 10 patients using extended wear contact lenses. The same regimen was also followed for five days by 25 patients using daily wear soft contact lenses. The contact lenses and their soaking solutions were collected at the end of the wearing period and analyzed for DSCG, benzalkonium chloride (BAK), 2-phenylethanol, and EDTA. The lens soaking solutions and eluates prepared from the lenses were tested. In the extended wear group, small amounts of DSCG were detected in both the eluates and the soaking solutions. In the daily wear group DSCG was detected in small amounts in the soaking solutions but not in the eluates. BAK, EDTA, and 2-phenylethanol were not detected in any of the eluates or soaking solutions. During the study, no side effects of DSCG were observed in any of the patients. In animal experiments, radioactive DSCG was applied once to a series of rabbit eyes. Four hours after administration of the labelled DSCG, the animals' tears, cornea, and aqueous humor were examined for DSCG. Of the instilled dose, approximately 0.2% was found in the cornea, and less than 0.04% was found in the aqueous humor. We conclude that commercial DSCG applied topically to contact lenses does not result in the accumulation of either the drug or its preservatives in lenses and that DSCG can be safely applied directly onto a worn contact lens.

Animals↗
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Computer-aided interactive surgical simulation for craniofacial anomalies based on 3-D surface reconstruction CT images.

We developed a computer-aided interactive surgical simulation system for craniofacial anomalies based on three-dimensional (3-D) surface reconstruction CT imaging. This system has four functions: 1) 3-D surface reconstruction display with an accelerated projection method; 2) Surgical simulation to cut, move, rotate, and reverse bone-blocks over the reference 3-D image on the CRT screen; 3) 3-D display of the simulated image in arbitrary views; and 4) Prediction of postoperative skin surface features displayed as 3-D images in arbitrary views. Retrospective surgical simulation has been performed on three patients who underwent the fronto-orbital advancement procedures for brachycephaly and two who underwent the reconstructive procedure for scaphocephaly. The predicted configurations of the cranium and skin surface were well simulated when compared to the postoperative images in 3-D arbitrary views. In practical use, this software might be used for an on-line system connected to a large scale general-purpose computer.

Computer Graphics↗
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High-risk subgroup of inferior myocardial infarction: importance of anterior wall motion and right ventricular function.

To identify high-risk subgroups of inferior myocardial infarction, 75 patients presenting with their first inferior infarction were investigated by sequential gated blood pool scans. The patients were divided into four groups based on the right ventricular function (RVF) and anterior wall motion (AWM) of the left ventricle by scan at the time of admission. A second blood pool scan was performed at ten days to evaluate RV and LV function. Thirty-eight patients had cardiac catheterization before discharge and all patients were followed up for one year to determine their clinical outcome. Depressed RVF and reduced AWM were observed in 26 (35%) (Group A); depressed RVF and normal AWM were found in 20 (27%) (Group B); reduced AWM and normal RVF in 10 (13%) (Group C); and normal RVF and AWM in 19 (25%) (Group D). The mean values of biventricular function (LVEF, RVEF) in groups A, B, C, and D were (44.9 +/- 8.4%, 32.5 +/- 9.9%), (59.9 +/- 8.6%, 34.5 +/- 8.0%), (44.9 +/- 15.7%, 48.2 +/- 3.3%), and (60.4 +/- 9.1%, 51.6 +/- 10.6%), respectively, at admission. In serial measurements, LVEF did not change significantly in any group, however, RVEF improved nearly 10 points in groups A and B at 10 days. Group A also had the highest incidence (82%) of left anterior descending coronary artery involvement, and the highest mean creatine phosphokinase levels (762 +/- 318 U/l): Furthermore, group A had a high incidence of major complications during their hospital course and high mortality during the one-year follow-up. These data clearly identified group A as a high-risk subgroup of patients with inferior infarction.

Coronary Angiography↗
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Contribution of contractile state of the non-infarcted area to global ventricular performance after acute myocardial infarction: assessment by quantitative radionuclide angiography.

To evaluate the regional contractile state of the non-infarcted zone and to determine the contribution of this area to left ventricular (LV) performance, 112 patients (42 anterior and 70 inferior infarction) with their first acute myocardial infarction were investigated by radionuclide ventriculography at admission and 10 days after admission. Wall motion at the non-infarcted area was defined as hyperkinetic, normal, or hypokinetic, if radial chord shortening had above normal, normal, or below normal values, respectively, by quantitative wall motion analysis. Hyperkinetic, normal, and hypokinetic wall motion of the non-infarcted area were observed in three (7%), 12 (29%), and 27 (64%) patients in anterior infarction and 14 (20%), 28 (40%), and 28 (40%) in inferior infarction, respectively. In the patients with hypokinetic wall motion at the non-infarcted area, the infarct involved more than 30% of the left ventricle manifesting akinetic contractile segment (ACS), radial chord shortening in the infarcted area was severely depressed, and the incidence of multi-vessel involvement was higher compared with those with hyperkinetic or normal wall motion. In serial measurements, radial chord shortening in the infarcted and non-infarcted area, percent ACS, left ventricular ejection fraction, and left ventricular end-diastolic volume index did not change significantly from acute to follow-up study in any group. In conclusion, our data indicated that the non-infarcted area following acute infarction had various contractile states and these conditions were determined primarily by the severity and extent of infarct and underlying coronary artery disease. Furthermore, the contractile state of the non-infarcted area has a supplemental role in determination of LV function following acute infarction.

Adult↗
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HSP47 as a possible marker for malignancy of tumors in vivo.

We have developed a rat monoclonal antibody against mouse HSP47 (designated as NK47) and investigated the expression of HSP47 in several mouse ascites and solid tumors. The expression of HSP47 was not detected in ascites tumors such as P388 and Ehrlich ascites tumors while HSP47 was markedly expressed in solid tumors such as Colon-26, B16 and B16-BL6 melanomas. HSP70, HSC70 and HSP90 were expressed in almost the same level among all the tumors examined. These results suggest that the expression of HSP47 decreases as the tumor becomes more malignant.

Animals↗
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A new method of intra-arterial regional chemotherapy with more selective drug delivery for locally advanced pancreatic cancer.

BACKGROUND/AIMS: In order to deliver the anti-cancer drugs more selectively into the cancer tissues, we have developed a new method of intra-arterial regional chemotherapy for locally unresectable cancer of the exocrine pancreas. MATERIALS AND METHODS: This method involved placing the catheters selectively into the splenic artery and/or into the gastroduodenal artery during laparotomy. Postoperatively, via the catheter, we infused 50-100 mg of Methotrexate mixed with 10 micrograms of Angiotensin-II with an intent of increasing the blood flow in the tumor tissue but decreasing that to the non-tumor tissues. Simultaneously, a bolus iv-infusion of 5-fluorouracil (5-Fu, 500 mg) was performed. One day after each chemotherapy, citrovorum factor (Leucovorin, 30mg) was given per orally, For 15 patients with locally non-resectable pancreatic cancer, this treatment was repeated weekly or biweekly at our out-patient clinic. RESULTS: As a result, the toxicity was so slight that all patients could tolerate this treatment as long as the catheter was patent (11 +/- 8 postoperative months). The survival period was 16 +/- 9 months (range: 5-36 months; median: 14 months), and one-, two- and three- year survival rates were 60%, 23% and 11%, respectively. Patients could take care of themselves within 12 +/- 9 months, and either complete (50%) or partial (50%) pain-relief was obtained among the 12 patients with severe pain. Only one patient experienced local tumor regression during the chemotherapy, and the incidence of liver metastasis was as low as 13%. CONCLUSIONS: Comparing with the previously reported data in the traditional chemo- and/or radio-therapies, we consider that our method of intra-arterial chemotherapy is quite useful not only for the prolongation of patient's survival but also for improving the quality of life. Thus, this new treatment seems worthy of entering into the prospective randomized study.

Aged↗