An inexorable trend of and major avenues to the diversion of China's agricultural labour force.
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Agriculture↗
Biomedical subjects
T Xu
Publications and source records attributed to T Xu.
Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats.
The primary structure of the major embryonic Notch transcript is presented, as determined by sequence analysis of overlapping cDNA clones. The 10,148 bp sequence corresponding to this transcript possesses an 8109 bp open reading frame that potentially codes for a 2703 amino acid protein. We show that this polypeptide contains a repeated structure composed of 36 tandemly arranged 40 amino acid long repeats, which show homology to the epidermal growth factor and other proteins containing EGF-like repeats. Hydropathy plots suggest that the putative Notch protein may span the membrane. We relate these findings to the developmental action of Notch and speculate that the locus may be involved in a cell-cell interaction mechanism that is essential for the differentiation of the ectoderm into neural and epidermal precursors.
Amino Acid Sequence↗
Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences.
Two functional cytosolic thymidine kinase (tk) cDNA clones were isolated from a mouse L-cell library. An RNA blot analysis indicated that one of these clones contains a nearly full-length tk sequence and that LTK- cells contain little or no TK message. The nucleotide sequences of both clones were determined, and the functional mouse tk cDNA contains 1,156 base pairs. An analysis of the sequence implied that there is an untranslated 32-nucleotide region at the 5' end of the mRNA, followed by an open reading frame of 699 nucleotides. The 3' untranslated region is 422 nucleotides long. Thus, the gene codes for a protein containing 233 amino acids, with a molecular weight of 25,873. A comparison of the coding sequences of the mouse tk cDNA with the human and chicken tk genes revealed about 86 and 70% homology, respectively. We also isolated the tk gene from a mouse C57BL/10J cosmid library. The structural organization was determined by restriction mapping, Southern blotting, and heteroduplex analysis of the cloned sequences, in combination with a mouse tk cDNA. The tk gene spans approximately 11 kilobases and contains at least five introns. Southern blot analysis revealed that this gene is deleted in mouse LTK- cells, consistent with the inability of these cells to synthesize TK message. This analysis also showed that tk-related sequences are present in the genomes of several mouse strains, as well as in LTK- cells. These segments may represent pseudogenes.
Amino Acid Sequence↗
The simultaneous discharges of two neurons in N. Pf and RF and the influences on them by stimulating central gray matter.
This paper employs the method of simultaneous recording of the electric discharges of two neurons with two microelectrodes to study the interrelationship of simultaneous electric activities of pain excitation neurons (PEN) and pain inhibition neurons (PIN) in N. parafascicularis (Pf) of thalamus and reticular formation (RF) of midbrain in 42 rats. The results prove: (i) There are both PEN and PIN in RF in midbrain and they act coordinately; (ii) noxious messages may simultaneously act on PEN and PIN in N. Pf and RF, and the reciprocal coordination of electric activities of these two kinds of neurons is very obvious; (iii) stimulating central gray matter or injecting morphine may weaken the activities of PEN simultaneously, with the opposite effects on PIN.
Action Potentials↗
Small leucine-rich proteoglycans in the aging skeleton.
Small Leucine-Rich Proteoglyans (SLRPs) are major skeletal extracellular matrix (ECM) components that comprise a family of 13 members containing repeats of a leucine-rich motif. To examine SLRP function, we generated mice deficient in one or more member and analyzed them at the tissue, cell and molecular levels. This review outlines the novel research findings uncovered using these new animal models.
Aging↗