Biomedical subjects

T Wing

Publications and source records attributed to T Wing.

13 recordsLinked to original sources

PubMed2005

Biochemical evaluation of mitochondrial respiratory chain in duodenum of low and high feed efficient broilers.

Increased H2O2 production, indicating higher oxidative stress, and lower mitochondrial function was previously observed in duodenal mitochondria isolated from broilers with low feed efficiency (FE, gain:feed). Thus, experiments were conducted to 1) evaluate the activity of the respiratory chain complexes (complexes I to V) and 2) assess protein oxidation and mitochondrial protein expression in broilers with low and high FE. Duodenal mitochondria were isolated from broiler breeders with low (0.52 +/- 0.01) and high (0.68 +/- 0.01) FE (n = 8/group). Respiratory chain complex activities were measured spectrophotometrically, whereas mitochondrial protein expression and protein oxidation (carbonyls) were assessed with Western blots. The activities of all complexes, except complex IV, were lower in the low FE compared with high FE mitochondria, whereas protein carbonyl levels were higher in low FE mitochondria. Steady-state levels of 6 out of 7 nuclear-encoded respiratory chain subunits [70S(FP), core I, core II, cytochrome c (cyt c)1, iron-sulfur protein (ISP), and ATPase-alpha] were higher, whereas 3 out of 6 mitochondrial-encoded subunits (ND4, ND6-C, and COX II) were lower in the low FE group, suggesting that sensitivity of mitochondrial proteins to H2O2 or oxidation varies. The general reduction in complex activity and differential protein expression concomitant with higher oxidized proteins in low FE mitochondria suggest that oxidative stress could be contributing to the lower mitochondrial function observed in low FE duodenal mitochondria.

Animal Feed↗

PubMed2005

Glutathione and respiratory chain complex activity in duodenal mitochondria of broilers with low and high feed efficiency.

We previously observed increased reactive oxygen species (ROS) production in intestinal mitochondria obtained from broiler breeder males with low feed efficiency (FE, gain-to-feed). Because antioxidants are critical for combating ROS-mediated oxidative stress and preserving mitochondrial function, the objectives of this study were 1) to determine levels of reduced glutathione (GSH), a major antioxidant in mitochondria, 2) to measure activities of GSH recycling enzymes: GSH peroxidase and GSH reductase, and 3) to establish relationships between antioxidants and respiratory chain complex activities (complexes I, II, III, IV, and V) in broiler breeder males with low and high FE. Duodenal mitochondria were isolated from broilers with low (0.62 +/- 0.01, n = 8) and high (0.80 +/- 0.01, n = 8) FE. Activities of respiratory chain complexes, GSH peroxidase, and GSH reductase, and levels of GSH were measured by UV spectrophotometry. There were no differences in GSH peroxidase or reductase activities or in individual complex activities between groups but GSH levels tended to be higher (P = 0.075) and oxidized to reduced glutathione ratio tended to be lower (P = 0.077) in broilers with high FE. Regression analysis revealed significant correlations (P < or = 0.05) between mitochondrial GSH and activities of complexes II, IV, and V with R2 values of 0.35, 0.56, and 0.49, respectively. These data suggest that GSH may be important in maintaining or enhancing the activity of certain respiratory chain complexes and could be involved in the phenotypic expression of feed efficiency in broilers.

Animals↗

PubMed2005

Compromised liver mitochondrial function and complex activity in low feed efficient broilers are associated with higher oxidative stress and differential protein expression.

Variations in broiler growth and efficiency have been explained in part by differences in mitochondrial function and biochemistry in broilers. To further our knowledge in this regard, 2 experiments were carried out to determine the relationships of a) mitochondrial function and activities of various electron transport chain (ETC) complexes; b) production of H2O2, a reactive oxygen species (ROS), and its association with protein oxidation; and c) mitochondrial protein expression in liver of a single line male broilers with low or high feed efficiency (FE, n = 5 to 8 per group). Mitochondrial function and complex activities were measured polarographically and spectrophotometrically, respectively. H2O2 was measured fluorimetrically, whereas oxidized protein (carbonyls) and specific mitochondrial proteins were analyzed using Western blots. Mitochondrial function (ETC coupling) and activities of ETC complexes (I, II, III, and IV) were higher in high FE compared with low FE broilers. H2O2 and protein carbonyls were higher in the livers of low FE broilers than in high FE broilers. Whereas the expression of 4 immunoreactive proteins [NAD3 (complex I), subunit VII (complex III), cytochrome c oxidase subunits (COX) II, and COX IVb (complex IV)] were higher in low FE liver mitochondria and 2 proteins [subunit 70 (complex II) and a-ATP synthase (complex V)] were higher in high FE birds, there were no differences between groups in the expression of 18 other mitochondrial proteins. In conclusion, increases in oxidative stress in low FE broilers were caused by or may contribute to differences in mitochondrial function (ETC coupling and complex activities) or the differential expression of steady-state levels of some mitochondrial proteins in the liver. Understanding the role of oxidative stress in Low FE broilers will provide clues in understanding the cellular basis of feed efficiency.

Animal Feed↗

PubMed2005

Longitudinal multiple-trait versus cumulative single-trait analysis of male and female fertility and hatchability in chickens.

The objective of the current study was to compare the performances of a longitudinal multipletrait (MT) and cumulative single-trait (ST) best linear unbiased prediction (BLUP) analyses for eggs set (E), percentage fertility (F), and percentage hatched of fertile eggs (H) based on rank correlations of hens and roosters (ROO). Longitudinal data consisted of weekly records, and the ST analysis used cumulative records of the 3 traits. Comparisons were carried out using simulated data, in which true values assigned during the simulation were compared with estimates obtained from MT and ST analyses. Two methods were used to calculate breeding values and ROO effects. Weekly E records equal to zero were assumed as valid records, and missing weekly F and H records were predicted in MT analysis. However, missing F and H were not included in the cumulative record. Weekly E, F, and H records were converted to a cumulative record following a procedure used by a commercial broiler company. Further, ST models were developed to evaluate hens and ROO separately as currently practiced in the industry. There was virtually no difference in hen rank correlations between true and predicted breeding values obtained from MT (0.64) and ST (0.62) analyses for E, thus suggesting that hens would rank similarly when weekly or cumulative data were used for genetic evaluation of E. With ST analysis, rank correlations between true and estimated hen breeding values decreased, compared with MT analysis, by 74 and 75% for F and H, respectively. For F (H), the rank correlation between true and estimated ROO effects were 0.64 (0.67) and 0.17 (0.12) with MT and ST analyses, respectively. The results suggested that longitudinal MT BLUP, which handles missing records, was better at estimating true hen and ROO effects than ST BLUP. Therefore, it was recommended that the MT BLUP method be used for genetic evaluation of hens and ROO for E, F, and H.

Animals↗

PubMed2004

Low feed efficient broilers within a single genetic line exhibit higher oxidative stress and protein expression in breast muscle with lower mitochondrial complex activity.

The objectives of this study were to determine the effects of low or high feed efficiency (FE) on a) protein oxidation, b) the activities of various respiratory chain complexes, and c) expression of various mitochondrial proteins in male broilers within a single genetic line. Tissue homogenate or mitochondria were isolated from breast muscle of broilers with high (0.80 +/- 0.01) and low FE (0.62 +/- 0.02). The complex activities were measured spectrophotometrically, and the levels of oxidized protein (carbonyl) and immunoreactive mitochondrial proteins were analyzed using Western blots. Protein carbonyl levels were higher in low FE compared with high FE broilers breast muscle, which indicated enhanced protein oxidation in low FE mitochondria. Activities of all respiratory chain complexes (I, II, III, IV) were higher in high FE compared with low FE broilers for breast mitochondria. Whereas the expression of immunoreactive proteins was higher in low FE muscle mitochondria for 5 mitochondrial proteins [core I, cyt c1, cyt b (complex III), COX II (cytochrome c oxidase subunit II, complex IV), and adenine nucleotide translocator (ANT1)], there were no differences between groups in the expression of 9 other respiratory chain protein subunits associated with complexex I, II, III, IV, and V. SDS-PAGE revealed a protein band of 47 kDa that was expressed at a higher level in low FE compared with high FE mitochondria. The differential expression of certain mitochondrial proteins and the 47-kDa band might be a compensatory response either to the lower complex activities or increased protein oxidation observed in low FE birds.

Animal Nutritional Physiological Phenomena↗

PubMed2004

Male and female fertility and hatchability in chickens: a longitudinal mixed model approach.

The objective of the current study was to investigate different approaches for handling missing records and to develop and implement a multivariate longitudinal mixed model for the genetic evaluation of male and female fertility and hatchability in chickens. The traits recorded on a weekly basis were eggs set (E), percentage fertility (F), and percentage hatch of fertile eggs (H). Three approaches for handling missing records were investigated: 1) all records with zero weekly laid eggs were removed and remaining records with missing F and H were predicted (M1); 2) missing records, including zero weekly laid eggs, were assumed known and equal to zero (M2); and 3) zero weekly laid eggs were assumed as a valid record and missing F and H were predicted (M3). A longitudinal mixed model was used for the multiple trait analysis of E, F, and H. Fixed effects included week-flock, age of service sire (rooster in the pen at data collection), and age of hen. Unrelated service sire, additive hen, permanent environmental, and the residual term were included as random effects. Heritability estimates ranged from 0.06 to 0.13 for E, F, and H. Heritability estimates of F and H were overestimated using M2. Correlations among the 3 traits were highest using M2 and lowest using M1. Pearson correlations indicated that reranking of birds and service sires could occur when using M1. Therefore, M1 should not be used for the analysis of longitudinal data of fertility and hatchability in chickens. Furthermore, M3 seems to be the most ideal method for handling missing records.

Analysis of Variance↗

PubMed2004

Determination of mitochondrial function and site-specific defects in electron transport in duodenal mitochondria in broilers with low and high feed efficiency.

Duodenal mitochondria were isolated from broiler breeder males with high (0.79+/-0.01, n = 9) and low (0.63+/-0.02, n = 9) feed efficiency (FE) to assess relationships of FE with duodenal mitochondrial function and site-specific defects in electron transport. Sequential additions of adenosine diphosphate (ADP) resulted in 1) higher respiratory control ratio (RCR; an index of respiratory chain coupling) in high FE mitochondria provided succinate, and 2) higher ADP to oxygen ratio (ADP:O; an index of oxidative phosphorylation) in low FE mitochondria provided NADH-linked substrates (malate, pyruvate, or both). Basal electron leak, measured as H2O2 production, was greater in low FE mitochondria provided succinate (P = 0.08) or NADH-linked substrates. As H2O2 levels were elevated in low FE compared with high FE mitochondria by complex I (P+/-0.07) and complex II inhibition, the higher basal electron leak in low FE mitochondria was apparently due to site-specific defects in electron transport at complexes I and II. Elevations in H2O2 above basal levels indicated that high FE mitochondria may also exhibit electron transport defects at complexes I and III. Despite an ability to produce adenosine triphosphate (ATP) that was equal or superior to that demonstrated in high FE duodenal mitochondria, low FE mitochondria exhibited a greater inherent degree of electron leak. The results provide insight into the role that duodenal mitochondria play in the phenotypic expression of FE in broilers.

Adenosine Diphosphate↗

PubMed2002

Association of mitochondrial function with feed efficiency within a single genetic line of male broilers.

Studies were conducted to determine relationships between feed efficiency and mitochondrial function and biochemistry. After feed efficiency (FE; gain:feed) was determined in broiler breeder males between 6 and 7 wk of age, mitochondria were isolated from breast and leg muscle from birds with high FE (0.83+/-0.01, n = 6) and low FE (0.64+/-0.01, n = 7). Respiratory chain coupling, assessed by the respiratory control ratio (RCR), was greater in high FE breast, and leg mitochondria provided NADH-linked, but not FADH-linked, energy substrates. There were no differences, however, in the adenosine diphosphate to oxygen (ADP:O) ratio (an index of oxidative phosphorylation) when mitochondria were provided either energy substrate. Electron leak, as determined by generation of H202, was greater in the low FE than in high FE breast mitochondria. Electron leak increased following inhibition of electron transport at Complex I (with rotenone) and Complex III (with antimycin A) in low FE but not in high FE breast mitochondria. There were no differences in basal electron leak in leg mitochondria between groups, but H202 generation was elevated (P < 0.07) compared to basal values in low FE leg mitochondria after Complex I inhibition. The activities of Complexes I and II were greater in high FE breast and leg muscle mitochondria compared to those in low FE mitochondria. The results indicate that lower respiratory chain coupling in low FE muscle mitochondria may be due to lower activities of Complexes I and II and defects in electron leak and provide insight into cellular mechanisms associated with the phenotypic expression of feed efficiency in broilers.

Adenosine Diphosphate↗

PubMed1998

Evaluation of minimally invasive indices for predicting ascites susceptibility in three successive hatches of broilers exposed to cool temperatures.

Broilers from three consecutive hatches were exposed to cool temperatures to amplify the incidence of pulmonary hypertension syndrome (PHS, ascites). The largest apparently healthy individuals on Day 42 were evaluated using minimally invasive diagnostic indices [percentage saturation of hemoglobin with oxygen, hematocrit (HCT), heart rate, electrocardiogram (ECG) Lead II, body weight), then they were subjected to the ongoing pressures of fast growth and cool temperatures to determine which of these indices are predictive of the subsequent onset of PHS. Approximately 20% of the males and females evaluated on Day 42 subsequently developed PHS by Day 51. When data for all hatches were pooled and broilers that subsequently developed ascites were compared with those that did not (nonascitic), body weights, heart rates, and percentage saturation of hemoglobin with oxygen were lower on Day 42 for ascitic than for nonascitic males, and HCT was higher in ascitic males and females than in nonascitic males and females, respectively. Comparisons of the ECG Lead II wave amplitudes for all hatches pooled indicated that RS-wave amplitude was larger in ascitic than in nonascitic males, and that S-wave amplitude was more negative in ascitic males and females than in nonascitic males and females. Necropsies conducted on Day 51 revealed higher right:total ventricular weight ratios in ascitic than in nonascitic broilers, whereas normalizing the left ventricle plus septum weight for differences in body weight generated similar values for ascitic and nonascitic males and females, respectively. These results support a primary role for pulmonary hypertension but not cardiomyopathy in the pathogenesis of ascites triggered by cool temperatures. Values obtained for minimally invasive diagnostic indices on Day 42 also establish predictive thresholds that can be used to evaluate the PHS susceptibility of large and apparently healthy male and female broilers.

Animals↗

PubMed1998

Effect of genetics, vaccine dosage, and postvaccination sampling interval on early antibody response to Salmonella enteritidis vaccine in broiler breeder chicks.

Broiler breeder chicks of two different genetic lines were evaluated for early antibody response to Salmonella enteritidis (SE) vaccine. Antibody responses to three dosages of SE vaccine administered at 22 d of age were measured at 3, 6, and 10 d postvaccination. Within each line, antibody levels at 10 d postvaccination were significantly higher than at either 3 or 6 d postvaccination. At all vaccine dosages, there was a significant antibody-response difference between the genetic lines at 6 and 10 d postvaccination. The vaccine dosage significantly affected antibody levels in one of the two genetic lines. These results demonstrate a genetic component of early antibody response to SE vaccine in broiler breeder chicks.

Analysis of Variance↗

PubMed1985

The delayed and saline wattle reactions in broilers challenged with bovine serum albumin.

Bovine serum albumin was used to study the development of a wattle reaction in broiler-type chickens. A strong response developed in both nonsensitized and sensitized chickens and the frequency of such was shown to be dependent on the method used for the sensitization. A similar swelling developed in a proportion of the wattles injected with saline and this too seemed to be influenced by the sensitization method. Moreover, both types of responses were shown to be somewhat unstable during the first 24 hr postchallenge.

Animals↗

PubMedDate not supplied

Salmonella enterica serovar enteritidis burden in broiler breeder chicks genetically associated with vaccine antibody response.

The relationship between antibody response to Salmonella enteritidis vaccine and internal organ burden of S. enteritidis is not fully understood. The genetic relationship, therefore, between postchallenge S. enteritidis burden and antibody response to S. enteritidis vaccine was determined in broiler breeder chicks. Sibling chicks from a broiler breeder male line were either inoculated with a pathogenic S. enteritidis or vaccinated with a commercial S. enteritidis vaccine. Spleen, liver, cecal wall, and cecal content samples from S. enteritidis-challenged chicks (n = 120) were cultured for enumeration of bacteria. Unchallenged chicks (n = 314) were vaccinated at 11 days of age, and serum samples were taken at 10 days postvaccination. Antibody response to vaccination and number of S. enteritidis in cecal content cultures were negatively correlated (-0.772), demonstrating that genetic potential for greater antibody response to S. enteritidis vaccine is associated with lesser S. enteritidis bacterial burden in cecal content of broiler breeder chicks. The findings suggest that genetic selection for vaccine antibody responsiveness can lower bacterial burden in the gut lumenal content and, thus, potentially reduce contamination of poultry products at processing.

Animals↗

PubMedDate not supplied

Kinetics of the wattle reaction to human plasma.

Human plasma produced cutaneous basophil hypersensitivity when injected into chicken wattles. The kinetics of the response development were affected by presensitization, whether or not complete Freund's adjuvant was used. Presensitized chickens developed their maximum response significantly sooner (12.6 hr earlier) than the controls.

Animals↗