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Biomedical subjects

T Wehner

Publications and source records attributed to T Wehner.

9 recordsLinked to original sources
PubMed2000

Co-operation at work: a process-oriented perspective on joint activity in inter-organizational relations.

In this paper the authors present a conceptual framework for analysing co-operation between organizations as a situated process, highlighting co-operation, co-ordination and co-construction as different modes of joint activity. Within this framework, the analysis of unexpected events becomes a crucial issue. The analytic power of reconstructing the (psycho-) logics of unexpected events in inter-organizational relations will be demonstrated in the presentation of selected outcomes of a research project on the dynamics of producer-supplier relationships in the German automobile industry. Also in this empirical part, the significance of trust and confidence in inter-organizational relations will be reflected.

Automobiles↗
PubMed2000

A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoproteins.

Borna disease virus (BDV) is unique among the non-segmented negative-strand RNA viruses of animals and man because it transcribes and replicates its genome in the nucleus of the infected cell. It has recently been discovered that BDV expresses a gene product of 87 amino acids, the p10 protein, from an open reading frame that overlaps with the gene encoding the viral p24 phosphoprotein. In addition, the p10 protein has been localized to intranuclear BDV-specific clusters containing viral antigens. Here, characterization of p10 interactions with the viral nucleoprotein p38/p39 and the p24 phosphoprotein is reported. Immunoaffinity chromatography demonstrated the presence of high-salt stable complexes of p10 containing the p24 and p38/p39 proteins in extracts of BDV-infected cells. Analyses in the yeast two-hybrid system and biochemical co-precipitation experiments suggested that the p10 protein binds directly to the p24 phosphoprotein and indirectly to the viral nucleoprotein. Mutational analysis demonstrated that a leucine-rich stretch of amino acids at positions 8-15 within the p10 protein is critical for interaction with p24. Furthermore, binding of p10 to the viral phosphoprotein was shown to be important for association with the BDV-specific intranuclear clusters that may represent the sites of virus replication and transcription in infected cells. These findings are discussed with respect to possible roles for the p10 protein in viral RNA synthesis or ribonucleoprotein transport.

Binding Sites↗
PubMed1997

Detection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues.

Borna disease (BD) is a transmissible, progressive polioencephalomyelitis primarily of horses and sheep. The genomes of two cell-adapted strains of Borna disease virus (BDV), the aetiological agent of BD, have been cloned and sequenced. According to the structural characterization achieved so far, BDV contains a non-segmented negative-sense 8.9 kb single-stranded RNA genome. In this paper we report the expression, purification and intracellular tracing of a novel non-glycosylated BDV-specific protein with a molecular mass of approximately 10 kDa (BDV p10 protein). The successful isolation of the corresponding mRNA from infected cells, amplification of the genetic region by RT-PCR and its efficient expression as a glutathione S-transferase (GST) fusion protein demonstrated that antibodies specific for the BDV p10 protein are induced in infected animals. In addition, we have produced monospecific antisera against the GST-p10 fusion protein in rabbits. This monospecific antiserum recognized the BDV p10 protein in brain cells of naturally and experimentally infected animals as well as in persistently BDV-infected cells. Antibody-mediated affinity-chromatography using the anti-p10 serum could successfully be applied to purify a ca. 10 kDa antigen from infected animal cells to such an extent that glycosylation of this component could be ruled out.

Animals↗
PubMed1986

Direction and frequency specific processing in the perception of long-range apparent movement.

The presentation of repetitive apparent movement (AM) produces the illusion of smooth sinusoidal movement. During prolonged observation this impression vanishes periodically. On the basis of these observations, it was postulated that such long-range AM are processed via direction and frequency specific subsystems. This hypothesis was examined by means of selective adaptation. A bi-stable movement display was used to measure the adaptation effect. Generally a frequency specific reaction for horizontal and vertical movement was verifiable. In addition to this, the existence of the presumed narrow-band processing channels was confirmed by the tuning curves determined. The significance of the results for AM perception and their applicability to the processing of real movement Gestalt will be discussed.

Adaptation, Ocular↗
PubMed1981

[Empirical comparative study on attitudes of dysmelic and non-disabled young people in the process of choice of occupation (author's transl)].

The present study deals with the question whether there are differences between disabled (n = 83) and non-disabled (n = 32) subjects concerning their ratings of (a) their subjective confidence in their potential for occupational activities and (b) the anticipated requirements of their intended jobs. No statistical differences have been found. Thalidomide-affected subjects however tend to choose an occupation the anticipated requirements of which are identical with their own subjective confidence in potential skills while ablebodied subjects ascribe themselves a wider range of potential skills their intended jobs. No statistical differences have been found. Thalidomide-affected subjects however tend to choose an occupation the anticipated requirements of which are identical with their own subjective confidence in potential skills while ablebodied subjects ascribe themselves a wider range of potential skills their intended jobs. No statistical differences have been found. Thalidomide-affected subjects however tend to choose an occupation the anticipated requirements of which are identical with their own subjective confidence in potential skills while ablebodied subjects ascribe themselves a wider range of potential skills and abilities, beyond those required by the chosen job. The second emphasis of the study dealt with the effects of the "induced" occurrence of events unrelated to the training itself on the remaining preparedness for activity (frustration tolerance). Disabled subjects demonstrate significantly higher frustration ratings in the "reactions of attachment figures" factor than the control subjects. This does however not diminish their preparedness for activity (i.e. to train for the chosen occupation). The study is based on the choice of occupation behaviour questionnaires by Bender-Szymanski (1976).

Adolescent↗
PubMed1976

Finding condensed descriptions for multi-dimensional data.

We describe two programs that may be used to find condensed descriptions for data available in a contingency table or in a covariance matrix in the case that these data follow a multinomial or a multivariate normal distribution, respectively. The programs perform a stepwise model search among multiplicative models by computing appropirate likelihood-ratio test statistics.

Computers↗