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Biomedical subjects

T Webb

Publications and source records attributed to T Webb.

At least 127 records · Page 7Linked to original sources
PubMed1977

Absence of cell-mediated immunity to rubella virus 5 years after rubella vaccination.

The long-term effectiveness of rubella vaccination in childhood is particularly important because the ultimate goal of immunization is the prevention of infection during pregnancy. Of 25 healthy children tested 4 to 5 years after rubella vaccination, 19 showed no evidence of cell-mediated immunity (CM) to rubella virus despite the presence of hemagglutination-inhibition or complement-fixation antibodies or both. Twenty-two of 25 seropositive, naturally infected young adults showed evidence of CMI. These results indicate that fetuses of women who have been vaccinated against rubella may not be protected against damage by wild rubella infection during the pregnancy, when CMI is physiologically depressed.

Adult↗
PubMed1977

A lack of correlation between 'T' antigen and any particular human chromosome in hybrids made between SV40 transformed human fibroblasts and mouse LMTK cells.

Lines of hybrid cells were established by fusion of mouse LMTK- cells with SV40 transformed human fibroblasts. The lines were examined for the presence of SV40 'T' antigen and the human chromosomes they carried identified by Giemsa banding. No significant correlation could be found between any particular human chromosome and the presence of SV40 virus.

Antigens, Viral↗
PubMed1977

Sulphated acid mucopolysaccharides in SV40-transformed human cells from normal and mucopolysaccharidosis patients.

Lines of fibroblasts have been established from normal individuals and from patients diagnosed as suffering from one of the mucopolysaccharidoses or mucopolysaccharide-storage diseases. Transformation of these lines with SV40 virus has been found to reduce their capacity to secrete sulphated mucopolysaccharides into the growth medium. No differences were detected between the individual cell types in their secretory capacity, either before or after viral transformation. A direct relationship was found to exist between the rate of acid mucopolysaccharide production and cell-doubling time. The level of sulphated mucopolysaccharide detected within the cell was also reduced for all cell types after transformation by SV40. Transformed fibroblasts from mucopolysaccharidosis patients, however, showed a relatively greater reduction in storage capacity than those derived from normal individuals.

Cell Line↗
PubMed1977

Chromosome complement and SV40 transformation of cells from patients susceptible to malignant disease.

A comparative study has been made of fibroblasts obtained from patients with differing susceptibilities to malignant disease, both with respect to their chromosome complements and their transformation with SV40 virus. Fibroblasts from 2 Bloom's syndrome patients were found not to have raised SV40 transformation rates and no correlation was found between chromosome abnormality per se and transformation. Of 2 cell types with greatly increased rates, one was derived from a neurofibromatosis patient and the other from an A-T heterozygote. When SV40 DNA was employed as the transforming agent for the latter, the transformation rate was no longer raised.

Antigens, Viral↗
PubMed1977

The relative ability of four rubella antigens to elicit blast cell transformation of lymphocytes from immune individuals.

The ability of crude, semi-purified, and purified rubella antigens to elicit a specific and significant blast cell transformation of lymphocytes from immune individuals was investigated in 25 seronegative and 25 seropositive young adults. The type of preparation and the purity of the antigens were critical. Titration of the antigens by complement fixation or hemagglutination was of little value for selecting the best antigen which was a whole virion-purified antigen.

Adult↗
PubMed1977

The chromosome analysis and susceptibility to transformation by Simian Virus 40 of fibroblasts from ataxia-telangiectasia.

Chromosome analyses are reported for 14 lines of fibro-blasts derived from 8 ataxia-telangiectasia (ATT) patients and for 14 lines of control cells. An elevated incidence of chromosome damage including gaps and breaks, rings, dicentrics and fragments, and chromosome figures has been found to occur in ATT cells. Similar abnormalities present in different cell lines suggest that common break points occur in ATT fibroblast chromosomes. The SV40 virus transformation rates found for ATT cells lie within the range found for normal cells, and there is no direct correlation between the degree of chromosome damage exhibited by any ATT cell line and its transformation rate with SV40.

Ataxia Telangiectasia↗
PubMed1976

Studies on the relationship between concanavalin A and SV40-transformed human fibroblasts.

The extent of binding of 125I-Con A to the surface of SV40-transformed human fibroblasts and the degree of agglutination of the cells by the native lectin have been measured. In addition, trypsinized and succinylated Con A have been used to study the effects of the lectin upon certain cell growth parameters. Trypsinized Con A was found to alter the growth rate, the saturation density and the contact inhibition of the transformed cells, an effect not neutralized by alph-methyl-D-mannoside.

Agglutination↗
PubMed1976

The transformation by simian virus 40 of cells from patients with mucopolysaccharidosis and from normal controls.

Fibroblasts derived from individuals with mucopolysaccharidosis, an inborn error of metabolism, have been found to be more easily transformed by simian virus 40 than are cells derived from normal individuals. The increased susceptibility does not seem to depend upon changes in glycoprotein at the cell surface. Repeated observations were necessary to demonstrate these differences, and we do not believe that this test is suitable for routine screening for cancer susceptibility.

Adsorption↗