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Biomedical subjects

T Waris

Publications and source records attributed to T Waris.

81 records · Page 5Linked to original sources
PubMed1977

Simultaneous demonstration of cholinesterases and glyoxylic acid-induced fluorescence of catecholamines in stretch preparations.

A combined simultaneous method to demonstrate adrenergic nerves using glyoxylic acid-induced fluorescence and nerves showing cholinesterase activity using the thiocholine technique is described in whole-mount preparations. The subcutaneous fascia and the right atrium of the heart of the rat and guinea-pig were used as tissue specimens, and the innervation patterns of adrenergic and cholinergic nerves were demonstrated in UV and transmitted light. Technical points and the limitations of the method are discussed.

Animals↗
PubMed1976

Innervation of human axillary sweat glands. Histochemical and electron microscopic study of hyperhidrotic and normal subjects.

Adrenergic nerves were demonstrated by formaldehyde-induced fluorescence and cholinergic nerves using thiocholine techniques to demonstrate acetylcholinesterase activities at the light and electron microscopic levels. The specimens were taken during surgery from the axillae of hyperhidrotic patients and normal voluntary controls. No fluorescent nerves were found around eccrine or apocrine sweat glands in hyperhidrotic or normally sweating axillae. Both eccrine and apocrine sweat glands exhibited a nerve network showing acetylcholinesterase activity. There was no marked difference in the innervation patterns or in the intensity of the acetylcholinesterase reaction of the nerves in the hyperhidrotic patients or normal subjects. Ultrastructurally the acetylcholinesterase-positive nerves were seen in the vicinity of both eccrine and apocrine glands, but these nerves were outside the basement membrane.

Axilla↗
PubMed1976

Combined trypan blue and formaldehyde-induced fluorescence in experimental skin freezing in the rat.

In order to study the effects of skin freezing in the rat on cutaneous vasculature and adrenergic nerves, a combination of trypan blue and formaldehyde-induced catecholamine fluorescence was used. It was shown that trypan blue clearly demonstrates both hyperaemic and ischaemic areas as well as changes in blood cessel permeability after experimental cold injury. Combination of the two fluorescence methods made it possible to correlate rough changes in blood vessel flow and permeability with changes in adrenergic nerves. Twentyfour hours after freezing of the skin, denervation of the most seriously damaged area was apparent. At the border of the cold injury, signs suggesting accumulation of actecholamines were observed in the subcutaneous nerves.

Animals↗
PubMed1975

Ultrastructural changes in the rat carotid body in severe haemorrhagia.

Rat carotid bodies were studied electron microscopically after short-term severe hypovolaemia, which is known to induce a marked chemoreceptor activation in the carotid body. Altogether 84 nerve-endings in the hypovolaemic rats' carotid bodies and 91 nerve-endings in the control carotid bodies were investigated. An increased accumulation of the glomus cell granular vesicles near the synaptic specializations of the nerve-endings was observed after hypovolaemia. Moreover, a statistically significant increase in the contacts between the nerve-ending synaptic specializations and the glomus cell granular vesicles was observed after hypovolaemia. A suggestion was made that the glomus cells might act as modulating, probably inhibitory, interneurones, whose catecholamines are responsible for the inhibition.

Animals↗
PubMed1975

Demonstration of catecholamines in peripheral adrenergic nerves in stretch preparations with fluorescence induced by aqueous solution of glyoxylic acid.

Fluorescence induced by aqueous solution of glyoxylic acid and formaldehyde-induced fluorescence of catecholamines were compared for the demonstration of peripheral adrenergic nerves in stretch preparations. Glyoxylic acid was better than formaldehyde for the demonstration of the adrenergic nerves. On the other hand, the formaldehyde was better than glyoxylic acid for the demonstration of biogenic amines in cell bodies.

Animals↗
PubMedDate not supplied

Congruence of adrenergic and cholinergic intrinsic innervation of human and rat atria.

Right atrial biopsies from rat and human hearts were studied using combined methods for the demonstration of glyoxylic acid-induced fluorescence (GIF) of catecholamines and acetylcholinesterase (AChE) reactions in the same specimens. In specimens from the rat heart, the GIF and AChE reactions were performed either simultaneously or consecutively. In biopsies of the human right atrium, obtained at right atria cannulation during open-heart surgery, the reactions were performed consecutively. It was found that in both rat and human atria the adrenergic nerves and AChE reactive nerves, which are probably cholinergic, run partly separately and partly together. In the rat atrial specimens, close relations between adrenergic nerves and clusters of AChE reactive cells were observed. In addition, clusters of fluorescent cells were observed in the vicinity of AChE reactive nerve bundles. This indicates that there may be several possible means of peripheral interaction between the intrinsic adrenergic and cholinergic systems of the rat heart.

Acetylcholinesterase↗
PubMedDate not supplied

Assessment of metabolic activity in aortic vein grafts in rats by histochemical examination of hydrolases.

The metabolic pattern of aortic vein grafts in rats was studied by estimating histochemically the activity of the hydrolytic enzymes alfa-esterase, aminopeptidase, adenosine triphosphatase, acid phosphatase and alkaline phosphatase. The enzyme activity was lowest in the 16 hour and 1 day old grafts, and recovery was noted at three days. Five days after transplantation the enzyme activities were higher than in the non-transplanted veins. The rapid increase in enzymatic activity found in histochemical studies on wound healing was not seen in these vein grafts. At four weeks some grafts showed intimal thickening the activity of which did not exceed that of the other layers of the graft wall. At the end of the observation period of sixteen weeks most of the grafts showed intimal thickening, and this layer stained intensely especially for ATPase. The staining pattern of most of the grafts at sixteen weeks resembled that of the aortic media.

Acid Phosphatase↗