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T Wang

Publications and source records attributed to T Wang.

At least 739 records · Page 41Linked to original sources
PubMed1989

[Study on the analgesic effect of acupuncture with opioid receptors agonist in induced arthritic rats].

Our previous study demonstrated that acupuncture increased pain threshold of the body, especially in the inflammatory area. Because acupuncture can promote the release of enkephalin in the brain, it is possible that analgesic effect of acupuncture could be further strengthened by administration of fentanylan opioid receptor's agonist and droperidol-dopamine receptor's agonist at same time. In present study arthritic rats induced by freund's complete adjuvant were divided into two groups. All of them received electro-acupuncture on bilateral "Hung-Tiao" points for 15 minutes. The first group animals were also given with common dose of fenanyl 10 micrograms/kg and droperidol 0.5 mg/kg by intraperitoneal injection. Pain threshold increased very soon after needling and drugs given. The analgesic effect lasted as long as 180 minutes. There is a statistical significant difference between this common dose group and acupuncture control group which only kept analgesic effect 90 minutes, P less than 0.01. When only 1/5 dose of these drugs with acupuncture was applied to arthritic rats, not only the pain threshold reached to 180 + 3% (P less than 0.01), but also the analgesic effect persisted as long as 120 minutes (P less than 0.01). The results from mentioned above suggested that if a small dose of drug is no effect in usual treatment, it could still play a role and reduce the pharmacological side effect by way of combining acupuncture application.

Acupuncture Analgesia↗
PubMed1989

Polymorphic DNA haplotypes at the phenylalanine hydroxylase (PAH) locus in Asian families with phenylketonuria (PKU).

DNA polymorphisms at the phenylalanine hydroxylase (PAH) locus have proved highly effective in linkage diagnosis of phenylketonuria (PKU) in Caucasian families. More than 10 RFLP sites have been reported within the PAH structural locus in Caucasians. With information from affected and unaffected offspring in PKU families it is often possible to reconstruct complete RFLP haplotypes in parents and to use these haplotypes to follow the segregation of PKU within families and to determine the distribution of PKU chromosomes within populations. To establish the utility of these RFLPs in characterizing Asian families with PKU, we typed eight DNA sites in 21 Chinese families and 12 Japanese families with classical PKU. The eight RFLPs were chosen for their informativeness in Caucasians. From these families we reconstructed a total of 91 complete PAH haplotypes, 44 from non-PKU chromosomes and 47 from PKU-bearing chromosomes. Although all eight marker sites are polymorphic in both Chinese and Japanese, there is much less haplotypic variation in Asians than in Caucasians. In particular, one haplotype alone, haplotype 4, accounts for more than 77% of non-PKU chromosomes and for more than 80% of PKU-bearing chromosomes. Haplotype 4 is also relatively common in Caucasians. The next most common Asian haplotype is 10 times less frequent than haplotype 4. By contrast, in many Caucasian populations the sum of the frequencies of the five most common haplotypes is still less than 80%, and several of the most common haplotypes are equally frequent. Even though the extent of haplotypic variation in Asians is severely limited, the few haplotypes that are found often differ at a number of RFLP sites.(ABSTRACT TRUNCATED AT 250 WORDS)

Alleles↗
PubMed1989

Whole-cell clamp study of Xenopus embryonic cholinergic neurons.

Whole-cell clamped myoballs are placed into direct visible contact with the growth cones of isolated neurons in embryonic Xenopus culture to serve as probe of acetylcholine (AcCHo) release in order to determine whether these neurons are cholinergic or not. Using a G omega -seal, whole-cell recording method, the electrophysiological properties of these identified cholinergic neurons are studied. It is found that these embryonic neurons, like adult frog motor neurons, exhibit repetitive firings in a certain embryonic developing stage. A development of repetitive firings is observed simultaneously. Tracing the development of one neuron, we find that the development of repetitive firing is completed at the 48th h after fertilization. Tetrodotoxin (TTX) which blocks Na+ channels can abolish all firings; and tetraethyl ammonium chloride (TEA), the blocker of K+ channels, reverses this development, i.e. it makes the repetitive firings disappear again. These data show that the nature of the development of repetitive firings is the development of K+ channels.

Acetylcholine↗
PubMed1989

Molecular genetics of phenylketonuria in Orientals: linkage disequilibrium between a termination mutation and haplotype 4 of the phenylalanine hydroxylase gene.

Phenylketonuria (PKU) is a common metabolic disorder among Chinese, with a prevalence of about 1 in 16,500 births. This frequency is very similar to that among Caucasians. Individual exons of the phenylalanine hydroxylase (PAH) gene with flanking introns were amplified by polymerase chain reaction and cloned into M13 for sequence analysis. An Arg111-to-Ter111 mutation has been identified in exon 3 of the PAH gene in a Chinese PKU patient. The mutation is in linkage disequilibrium with the mutant haplotype 4 alleles which are the most prevalent haplotype among the Orientals. The mutation accounts for about 10% of the Chinese PKU alleles and is absent from the Caucasians, demonstrating that independent mutational events have occurred in the PAH locus after racial divergence.

Asian People↗
PubMed1988

Increasing incidence and excellent survival of patients with early gastric cancer: experience in a United States medical center.

PURPOSE: In Japan, early gastric cancer has an excellent survival rate. In the United States, the disease is less well understood, and it is viewed more pessimistically, although we have previously shown in a small series of patients good short-term survival rates in early gastric cancer as compared with advanced gastric cancer. Our purpose in this study was to determine if the incidence of early gastric cancer and the associated survival rate has changed over a 24-year period. PATIENTS AND METHODS: From the records at the Columbia Presbyterian Medical Center, 549 patients were identified who underwent gastric resection for cancer between 1960 and 1984, 69 of whom had early gastric cancer. Survival data were obtained in 63 patients. A comparison of survival rates was conducted between patients with early gastric cancer and the 1980 census figures. RESULTS: Over the 24-year period, the total number of resections for gastric cancer at our institution declined. However, the percentage of gastric resections that satisfied the Japanese criteria for early gastric cancer increased from 9 percent between 1960 to 1974 to 17 percent between 1975 to 1984 (p less than 0.05). Of the 69 early gastric cancers, 35 percent involved the mucosa, whereas in 65 percent the malignancy invaded the submucosa. Twenty-eight percent had lymph node involvement. For the patients for whom survival data were available, survival was better than the 1980 census (p less than 0.05). The adjusted five-year survival rate was 97 percent. Neither submucosal invasion nor lymph node involvement altered survival. Patients with type III early cancers (ulcerated), however, had a significantly greater survival rate than patients with type I (polypoid) early cancers (p less than 0.05). CONCLUSION: Early gastric cancer is being diagnosed with increasing frequency and has an excellent survival rate. These findings are similar to the Japanese experience and argue for an ongoing aggressive approach to endoscopic biopsy of gastric lesions.

Aged↗
PubMed1988

The development and testing of intravenous dosing regimens: application to flecainide for the suppression of ventricular arrhythmias.

A two-part pharmacokinetic approach was used to prospectively develop and test intravenous flecainide infusion regimens for the acute therapy for ventricular arrhythmias. Initially, each of nine known responders to oral flecainide was given a rapid flecainide infusion to characterize pharmacokinetic parameters and determine the minimum effective concentration for each patient. These data were used to calculate individually appropriate three-stage flecainide infusions of predetermined durations in eight patients. The three-stage infusions (0.15 +/- 0.02 mg flecainide acetate/kg/min for 5 minutes, 0.046 +/- 0.004 mg/kg/min for 60 minutes, and 0.31 +/- 0.05 mg/kg/hr for 5 to 47 hours; mean +/- SE) resulted in 95% +/- 0.1% suppression of ventricular ectopic depolarizations. Based on these results, six additional patients received a uniform infusion regimen (0.1 mg/kg/min for 5 minutes, 0.025 mg/kg/min for 2 hours, and 0.25 mg/kg/hr for 46 hours). Supplemental doses of 0.25 mg/kg were given (four doses per patient). With this protocol, ventricular ectopic depolarizations were 82.6% +/- 8.5% suppressed. Measured plasma flecainide concentrations were not significantly different from those predicted by pharmacokinetic models. A prompt and sustained antiarrhythmic effect was obtained with an intravenous regimen of flecainide determined by a prospective pharmacokinetic approach. However, the dosages developed in this study may have to be modified for patients with impaired cardiac or renal function.

Adult↗
PubMed1988

Simple microbiologic detection of Campylobacter pylori.

A study of 84 gastric biopsies taken from 42 adult patients revealed simple techniques for Gram stain and culture for Campylobacter pylori. In an initial study of 18 biopsies, Gram stains prepared from ground, diluted tissue were all negative for curved, gram-negative rods, whereas 13 of these specimens were positive for C. pylori by culture. The Gram stains for the remaining 66 biopsies were prepared by a rinse-imprint technique. In this group, there were 30 Gram stains positive for organisms resembling C. pylori and 32 positive cultures. By Gram-staining two sites, fundus and antrum, the sensitivity of the Gram stain for identifying a positive specimen increased from 91 to 100%. Gram stain may be the preferred technique for rapid diagnosis. When cultured, C. pylori was recovered most often on modified Thayer-Martin medium incubated microaerophilically at 35 degrees C. The presence of antibiotics in modified Thayer-Martin medium limited upper respiratory flora overgrowth, which was often present on nonselective media.

Biopsy↗
PubMed1988

Cloning and sequencing of a beta-thalassemia gene from south China.

A beta-thalassemia gene from a patient with beta+-thalassemia in South China was cloned and sequenced. A four nucleotide (TCTT) deletion in codons 41 and 42 was found. This is the most common beta-thalassemia gene in South China and the first mutant identified on China mainland.

Amino Acid Sequence↗
PubMed1988

Regional pulmonary perfusion estimated by high-speed volume scanning CT.

Three pigs with surgically created aortopulmonary shunts and two pigs who had sham operation were scanned in a fast computed tomography (CT) scanner, the dynamic spatial reconstructor (DSR). Each pig was scanned during injection of a bolus of roentgen contrast medium in the right atrium and during injection into the left ventricle. In addition to these DSR scans, radiolabeled, 15-micron-diameter microspheres were injected into the right atrium and into the left ventricle. The quantitative distribution of roentgen contrast agent was used to estimate regional pulmonary blood flow independently from the right and left circulations using analysis of the indicator dilution curves derived from the DSR images (Y). These values were compared to the regional values of pulmonary perfusion based on the microsphere distribution (X). The correspondence between the two methods (for all pigs together) was Y = 0.83X + 0.52 with r = 0.964. The less than unity slope is attributed to the partial collapse of the lung at the time of sectioning (for counting of tissue radioactivities). As regional air content of the lung also was measured from the DSR images, these data support the contention that regional ventilation and perfusion can be estimated from a fast CT scan of the thoracic contents during the passage of a bolus of contrast medium.

Animals↗
PubMed1988

Angiotensin receptor mediated regulation of transport in the rat proximal tubule.

The intracellular mechanism of Ang II action, as supported by the present data, may be summarized by the following: Ang II interacts with its receptors, leading to an increase in cytosolic Ca+2 by mobilization of Ca+2 from intracellular pools. This rise in cytosolic Ca+2 initiates the activation of calmodulin-regulated enzymes. This then leads to inhibition of both JV and JHCO3-.

1-Sarcosine-8-Isoleucine Angiotensin II↗