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Biomedical subjects

T Wang

Publications and source records attributed to T Wang.

At least 649 records · Page 36Linked to original sources
PubMed1993

Immunopathological stain of lipoarabinomannan-B (LAM-B) for diagnosis of leprosy.

We developed an immunopathological staining of LAM-B antigen in formalin-fixed paraffin-embedded tissues, and compared it with, PGL-I immunostaining, Fite Faraco's stain and periodic acid carbol pararosaniline (PACPR) stain. Out of the total 28 leprosy cases, 27 were positive to LAM-B immunostaining while 23 were positive to PGL-I stain. Fite's stain was positive in 21 cases while PACPR stain was positive in 24 cases. In scrofuloderma, LAM-B antigen was observed only in the granuloma while no other positive findings were noted with other stains. Normal skin did not give any positive findings with any of the stains. Other dermatoses showed no positive findings to any of the stains tested. LAM-B staining was observed in the nerve even in the absence of bacilli in leprosy tissues. Presence of LAM-B in the cutaneous nerves is helpful in discriminating leprosy from other mycobacterioses. Considering the high sensitivity of LAM-B and the predilection of M. leprae for the nerves, we concluded that LAM-B staining can be a useful new tool in the prompt diagnosis of leprosy, especially in suspected or early cases.

Diagnosis, Differential↗
PubMed1993

[Defects in insulin binding, internalization and degradation of monocytes from patients with non-insulin-dependent diabetes mellitus].

A14-125I-monoiodoinsulin binding to, internalization into, and degradation inside human circulating monocytes, a cell type widely used for insulin receptor studied, from normal(32) and weight, age-matched NIDDM(31) subject were studied. The specific insulin binding, internalization and degradation were decreased in the cells from NIDDM compared to that in the cells from normal subjects. Scatchard plot analysis of these group data reveal that cells from the normal control subject contain 28,000 receptor sites per cell, while monocytes from the NIDDM patients contain 15,000 sites per cell. These results indicate that the decreased abilities of insulin binding, internalization and degradation of target cells may play a role in the cellular resistance to insulin that occurs in the NIDDM.

Adult↗
PubMed1993

[Comparison of quality of life in continuous ambulatory peritoneal dialysis and hemodialysis patients].

Quality of life is a important outcome of treatment of chronic disease. It's also a major factor to determine the selection of various modes of therapy for end-stage renal diseases (ESRD) patients. Quality of life may be influenced by some case mix factors. 17 continuous ambulatory peritoneal dialysis (CAPD) patients and 17 hemodialysis patients were closely matched on sex, age, initial disease, education, financial support, cormidity and marital status to compare their quality of life. There were no difference of quality of life between these two group. These results suggest that dialysis modality do not likely to exerts an independent effect on quality of life of chronic dialysis patients. It may be warrant to speed up the CAPD program due to its less expensive.

Adult↗
PubMed1993

[Line width addition method and its application to biologic materials emission spectrum analysis].

Using alternating current are grating spectrograph, and self-made recording microphotometer for line contour and accurate measurement of line width, we measured the concentration of Cu and Ca in serum-like synthetic standard solution with line width addition method. The experimental results had better linearity, reproducibility and higher precision compared with those by density method, suggesting that line width method is better than density method in the analysis of biologic material concentration.

Animals↗
PubMed1993

Misdiagnosis of lung cancer in patients under age 40--a clinical analysis of 47 cases.

Diagnosis of lung cancer in 47 patients aged younger than 40 years was delayed as a result of misdiagnosis, accounting for 70.1% of all cases of lung cancer in those aged less than 40 years treated in PUMC Hospital in the same period. Pulmonary tuberculosis and tuberculous pleurisy were diagnosed in 26 cases (55.3%). Pulmonary inflammation was suspected in 24 cases (51.1%). The delay in diagnosis was one to three months in 28 cases and longer than 3 months in 19 cases. Twenty-eight (59.6%) of the 47 patients underwent surgery, including exploratory thoracotomy (21.4%) and pneumonectomy (40.9%). The five-year survival rates in the resectable group and in the non-surgical group were 31.6% and 5.3%, respectively. We recommend that accessory examinations should be performed wherever possible in younger patients with suggestive pulmonary nodules in order to collect sufficient evidence to confirm or rule out the diagnosis of lung cancer and thereby reduce the rate of misdiagnosis in such younger people.

Adenocarcinoma↗
PubMed1993

[Effects of stimulating lateral hypothalamic area and ventromedial nucleus of hypothalamus on cerebellar cortical neuronal activity in the cat].

In pentobarbital anaesthetized cat, stimulating lateral hypothalamic area (LHA) and ventromedial nucleus (VMN) of the hypothalamus could elicit inhibitory, excitatory or inhibitory-excitatory responses of cerebellar Purkinje cells (PC) and non-Purkinje cells (NPC) in the loubles VI and VII. The majority of responses are inhibitory (80.4%) usually with a latency of 10-20 ms, or occassionaly as long as 90 ms. Usually stimulation of LHA was more effective and NPC was more responsive. The aforementioned inhibitory response could be blocked by iv. chlorpheniramine. The possible role of the hypothalamo-cerebellar activity was discussed.

Animals↗
PubMed1993

[Cytogenetic studies of brown field-mouse].

The karyotype of Brown Field-Mouse (Microtus mandarinus) is reported in this paper. The formula of karyotypes is:2n = 50 = 2 (M,T)+2SM+44T+XX(M,SM)XY(SM,ST). The first pair of the autosomes and the X-chromosomes show polymorphism. Only one X-chromosome can be found in the seven out of the fifteen female specimens studied. The formula of its karyotypes is 2n = 49 = 2(M,ST)+2SM+44T+X O. The X-chromosomes of these seven specimens are metacentric, and are different from the X (that is SM) of normal male, these are the type M. Three kinds of karyotypes of Microtus mandarinus studied in this paper are different from the karyotypes (XO,OY,XY) of M. oregoni studied by Brown et al. (1964). The mechanism of generation of chromosomal polymorphism is discussed. Individual ontogenesis mechanism of the type XO and its reproduction are also discussed.

Animals↗
PubMed1992

Replication-dependent and independent regulation of HMG expression during the cell cycle and conjugation in Tetrahymena.

Two abundant high-mobility-group (HMG)-like proteins, HMG B and HMG C, exist in the ciliated protozoan, Tetrahymena thermophila. Of these, HMG C is specific to transcriptionally active macronuclei, while HMG B is found in macronuclei and in transcriptionally inactive micronuclei [1]. Using Northern and in situ analyses, we show that the genes encoding HMG B and HMG C are not expressed uniformly throughout the vegetative cycle or during the sexual process, conjugation. Elevated expression of both genes is observed during macronuclear S phase of the vegetative cycle and during endoreplication of developing new macronuclei in later stages of conjugation. Interruption of any of these macronuclear DNA replications by aphidicolin leads to a rapid drop in the message levels of HMG B and HMG C. These results resemble what is typically observed for replication-dependent nucleosomal histones and differ from the apparent lack of cell cycle regulation observed for HMG genes in vertebrates. A specific-induction of HMG B mRNA is also observed early in conjugation and during this interval, inhibition of micronuclear DNA synthesis by aphidicolin does not affect the message level of HMG B. Thus, during conjugation, expression of HMG B shows both replication-dependent and independent regulation. Results similar to these with HMG B are obtained with histone H4II gene, a gene which is also expressed during micro- and macronuclear S phases during the vegetative cycle. These results demonstrate surprising complexity in the expression of HMG genes in Tetrahymena and lend support to the hypothesis that cell cycle regulation plays an important role in directing HMG-like proteins to the appropriate nucleus [2]. Interestingly, expression of neither HMG gene is perfectly synchronized with that of histone H4II gene during the developmental program suggesting that important differences exist between vegetatively growing (cell cycle control) and conjugating (developmental control) cells.

Animals↗
PubMed1992

Separation of serum bilirubin species by micellar electrokinetic chromatography with direct sample injection.

Four major bilirubin species in serum were separated by micellar electrokinetic chromatography with 25 mM sodium dodecyl sulfate (SDS) and 20 mM sodium tetraborate-boric acid buffer at pH 8.5. Due to the solubilization of the serum proteins by the SDS micelles, serum samples were injected directly into a 50 cm x 75 microns I.D. fused-silica capillary and complete separation of the four bilirubin species was accomplished within ca. 10 min without extensive sample pretreatment. Detection was performed by absorbance at 450 nm and average limit of detection was in the 6.0 microM concentration range. The usefulness of this method was demonstrated for the separation and detection of a number of bilirubin species present in pathological human serum samples.

Animals↗
PubMed1992

Involvement of opioid receptors in nucleus tractus solitarii in modulating endotoxic hypotension in rats.

Opioid antagonists selective for the mu-receptor (naltrexone) and kappa-receptor (nalmefene), respectively, were unilaterally microinjected into the nucleus tractus solitarii (NTS) of the pentobarbital anaesthetized (50 mg/kg, i.p.) rat subjected to E. coli endotoxin (15 mg/kg, i.v.). Naltrexone (0.5 microgram) ameliorated or totally abolished the endotoxic hypotension and tachycardia, and nalmefene at a dose of 1/25 (0.02 microgram) that of naltrexone showed a similar effect. The results suggest that the kappa- and mu-opioid receptors in NTS are actively involved in mediating the hypotensive and tachycardiac effects induced by systemic endotoxin in rats.

Animals↗
PubMed1992

MSA-36: a chromosomal and mitotic spindle-associated protein.

We have identified a novel M(r) 36,000 protein (MSA-36) that has a complex cell cycle dependent distribution. This protein is first detected in interphase nuclei just prior to the onset of chromosome condensation. MSA-36 is found along condensing chromosomes and is a component of the centromere through metaphase. At anaphase, this protein is no longer detected in association with the chromosomes but appears at the forming stembodies and subsequently within the intercellular bridge at either side of the midbody. At the completion of cell division, the amount of MSA-36 in the bridge appears to decline concurrent with the appearance of this protein briefly within the reforming nucleus. To investigate whether MSA-36 is an active component of the chromosome or a passive passenger protein, we studied the behaviour of this protein in cells exhibiting premature chromosome condensation and in cells during and following recovery from mitotic arrest. These studies suggest that MSA-36 is not essential for a variety of major chromosome-associated events.

Autoantigens↗
PubMed1992

Freezing preservation of the mammalian cardiac explant. V. Cryoprotection by ethanol.

We studied the colligative cryoprotective effect of ethanol (EtOH) in preserving the isolated rat heart frozen at -3.4 degrees C or unfrozen at -1.4 degrees C. Addition of 4.7% (v/v) EtOH to a cardioplegic solution, CP-14, raised the osmolality from 280 to 1100 mOsm/kg H2O and lowered the melting point from -0.52 to -2.1 degrees C. Freezing of the cardiac explant at -3.4 degrees C for 6 h resulted in 34.3 +/- 1.9% of the tissue water as ice; recovery of cardiac output (CO) was 50%. Polyethylene glycol, which at 5% (w/v) has been shown to cryoprotect the hearts during freezing at -1.4 degrees C, did not improve the protective effect of 4.7% EtOH. CP-14 + 4.7% EtOH did not freeze at -1.4 degrees C. After 6 h storage, CO in hearts flushed with CP-14 + 4.7% EtOH oxygenated with 95% O2/5%CO2 returned to almost control level and was much higher than that in hearts flushed with 100% O2 saturated-CP-14 + 4.7% EtOH. Storage of 8 and 12 h reduced CO to 87 +/- 9 and 60 +/- 5% of control. By employing EtOH as a colligative cryoprotectant, we preserved the adult mammalian heart frozen at -3.4 degrees C or unfrozen at -1.4 degrees C, suggesting that this small molecular weight, penetrating substance may be a suitable cryoprotectant for long-term storage of the cardiac explant at high subzero temperatures.

Animals↗
PubMed1992

Freezing preservation of the mammalian cardiac explant. VI. Effect of thawing rate on functional recovery.

This study investigated the effect of thawing rate on the preservation of frozen isolated rat hearts. The hearts were flushed with a hyperosmotic cardioplegic solution, CP-14/EtOH (1.15 Osm/kg), frozen at a rate of 0.18 degree C/hr for 6 h to -3.2 degrees C. Thereafter, the hearts were thawed at rates ranging from 0.08 to 1.1 degrees C/min for 1 to 14 min until the heart temperature reached -2.1 degrees C, the melting point (MP) of the flush solution; then they were held at -1 degree C for 11 to 24 min so that the total thaw time was 25 min. Post-thaw function was assessed by working reperfusion and expressed as percentage of unstored control function. Cardiac output (CO) and other hemodynamic performance showed biphasic responses to the thaw rate. At 0.08 degree C/min rate, CO recovered to 29.1 +/- 4.1 ml/min (40.8 +/- 5.8% of control). Thawing at 0.13 degree C/min enhanced the recovery of CO to 60.5 +/- 4.9%. Between 0.13 and 0.34 degree C/min, recovery was statistically insignificant. Faster thawing at 0.59 and 1.1 degrees C/min caused progressively less recovery. Overall, 0.13 degree C/min offered the highest recovery. In conclusion, function in slowly frozen heart is intimately affected by the thawing rate; there was an optimal intermediate thawing rate and both too slow and too fast thawing were detrimental.

Animals↗
PubMed1992

Centrally acting endogenous hypotensive substances in rats subjected to endotoxic shock.

Cerebroventricular perfusate (CVP) from rats subjected to endotoxic shock was infused into the lateral ventricle of the naive rat. This procedure produced a hypotensive response in the recipient rat which could be reversed by the intravenous injection of the opioid antagonist naltrexone. The degree of endotoxemic hypotension in the donor rat was attenuated by perfusing the cerebroventricular system with mock CSF. The results suggest the existence of endogenous hypotensive substances in rat central nervous system, possibly opioid in nature, which may play a critical role in the pathogenesis of endotoxic shock.

Animals↗
PubMed1992

Identification of three novel missense PKU mutations among Chinese.

Three novel missense mutations have been identified in the phenylalanine hydroxylase (PAH) genes of Chinese individuals afflicted with various degrees of phenylketonuria (PKU). A T-to-C transition was observed in exon 5 of the gene, resulting in the substitution of Phe161 by Ser161. Two substitutions, G-to-T and T-to-G, were observed in exon 7, resulting in the substitution of Gly247 by Val247 and Leu255 by Val255, respectively. Expression analysis demonstrated that these mutant proteins produced between 0 and 15% of normal PAH enzyme activity. Population screening of a Chinese sample population indicates that these mutations are quite rare, together accounting for only about 4% of all PKU alleles among the Chinese. The P161S and G247V mutations were each present on a single PAH RFLP haplotype 4 chromosome in patients form Northern China, while the L255V mutation was present on chromosomes of both haplotypes 18 and 21 in patients from Southern China. These results suggest that the remaining 30% of uncharacterized PKU alleles in the Chinese population may bear a large number of relatively rare PAH mutations.

Alleles↗