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Biomedical subjects

T Viswanatha

Publications and source records attributed to T Viswanatha.

At least 55 records · Page 3Linked to original sources
PubMed1975

Reversibility of the affinity labelled-biotin transport system in yeast cells.

Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotynyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling of the transport system.

Biological Transport↗
PubMed1975

The reaction of chymotrypsin with 2,3-butanedione trimer;.

A method for the preparation of the trimer of 2,3-butanedione has been developed; The reaction of this trimer with chymotrypsin A alpha was examined in the presence or absence of light. Under conditions of exclusion of light, modification of one to two arginine residues and of a similar number of lysine residues could be achieved without any loss of enzymatic activity. The trimer facilitated a rapid photoinactivation of the enzyme with little or no modification of the above amino acid residues. Such photoinactivation was not found to react with proflavine and diiosopropylfluorophosphate to an extent greater than that expected on the basis of residual activity presentmproflavine protected the enzyme from the trimer promoted photoinactivation.

Acridines↗