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Biomedical subjects

T Usui

Publications and source records attributed to T Usui.

At least 559 records · Page 31Linked to original sources
PubMed1982

Possible prevention from the progression of cardiotoxicity in adriamycin-treated rabbits by coenzyme Q10.

The cumulative dose-dependent cardiotoxicity induced by doxorubicin (adriamycin, ADR) and its possible prevention by coenzyme Q10 (CoQ10) were studied in rabbits. In the group that received ADR alone, ADR dose-dependent electrocardiography (ECG) abnormalities and severe myocardial damage on electron microscopic examination were observed. In the group that received ADR + CoQ10, these alterations occurred in lesser degree, and ECG changes seemed to be improved. The results indicated that CoQ10 might prevent the progression of cardiotoxicity in ADR treated rabbits.

Animals↗
PubMed1982

Copper levels in plasma and erythrocytes in healthy Japanese children and adults.

This study showed differences in plasma and erythrocyte copper concentrations in children and adults. All determinations were performed with a flameless atomic absorption spectrophotometer. The mean plasma copper level at 1 month of age was significantly lower than in adults. After 1 month of age the mean concentration in plasma increased to its peak value at 2 to 5 yr of age, then decreased gradually with age. At 7 months to 10 yr of age, the copper levels in plasma were significantly higher than in adults. The erythrocyte copper levels at 1 month to 1 yr of age were significantly higher than in adults. The copper content of erythrocytes was highest at 2 to 6 months of age and then decreased gradually. The copper concentration of erythrocytes may reflect more accurately liver and total body copper levels than does the plasma copper level. There is less possibility of copper contamination in erythrocytes than in hair. Therefore, the measurement of erythrocyte copper concentration may well be a helpful index of the total copper status.

Adolescent↗
PubMed1982

Inactivation of lysosomal enzymes by the respiratory burst of polymorphonuclear leukocytes. Possible involvement of myeloperoxidase-H2O2-halide system.

Lysosomal enzyme release from PMN during phagocytosis was examined in PMN from healthy subjects and patients with CGD. Normal PMN showed increased extracellular enzyme levels and a marked reduction of total (intracellular plus extracellular) enzyme activity after phagocytosis of STZ. In PMN from patients with CGD, a reduction of total enzyme activity was not observed and much more enzyme than normal was released extracellularly. We have examined the effect of the respiratory burst on enzyme activity mostly to loss of released enzymes. PMA-stimulated normal PMN inhibited the enzyme activity in postphagocytosis media from CGD PMN but PMA-stimulated CGD PMN did not. This inhibition by normal PMA-stimulated PMN was protected by the addition of catalase, histidine, and NaN3. Furthermore, these enzymes were inactivated by cell-free, glucose plus glucose oxidase (as a H2O2 generating system) and NaOCI. Histidine had a protective effect on the enzyme inactivation both by glucose plus glucose oxidase and by NaOCI. In contrast, histidine gave a slight increase of extracellular enzyme levels but failed to restore enzyme activity when added to the medium during phagocytosis in normal PMN. These evidences suggest that the inactivation of lysosomal enzymes is mainly due to MPO-H2O2-halide system, resulting in inhibition of lysosomal enzyme release from PMN during phagocytosis.

Enzyme Activation↗
PubMed1982

[Antibodies against human lymphocytes].

The introduction of hybridoma technology has greatly contributed to the identification and the characterization of a variety of cell surface antigens present on human lymphoid cells. Rapid progress has recently been made in generating monoclonal antibodies against human lymphoid cells and extensive studies in clinical medicine have defined the potential application of these monoclonal antibodies. In this review, we summarize the main similarities and differences among these monoclonal antibodies.

Animals↗
PubMed1982

Free amino acid content of lymphocytes nd granulocytes compared.

For extracting free amino acids from human leukocytes, we find that disruption of the cells by ultrasonication is more reliable than freezing and thawing. The amount of free amino acids extracted by the latter method depends on the temperature and duration of thawing. We extracted free amino acids from leukocytes of healthy men by ultrasonication and compared their concentrations in lymphocytes and granulocytes. The amino acid content of granulocytes significantly (p less than 0.001) exceeded that of lymphocytes. Of the amino acids extracted from granulocytes, 76% was taurine; for lymphocytes taurine, aspartic acid, and glutamic acid were predominant, respectively composing 44%, 17%, and 26% of the total. Taurine is proposed as an index of cell disruption.

Adult↗