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Biomedical subjects

T Ueno

Publications and source records attributed to T Ueno.

At least 325 records · Page 18Linked to original sources
PubMed1995

Transforming growth factor beta 1, extracellular matrix, and inflammatory cells in wound repair using a closed duodenal loop pancreatitis model rat. Immunohistochemical study.

BACKGROUND: Serial changes in the localization of various components of extracellular matrix in acute pancreatitis have been reported, but there have been no reports on serial changes in the localization of transforming growth factor beta and the determination of cells producing extracellular matrix. METHODS: In this study serial relationships between the localization of transforming growth factor beta 1, fibronectin and type-III collagen, inflammatory cells, and serum amylase levels in the process of tissue repair in acute pancreatitis were studied using a closed duodenal loop model rat. Furthermore, the cells producing transforming growth factor beta 1, fibronectin, and type-III collagen were investigated by immunoelectron microscopy. RESULTS: Three to 6 h after duodenal ligation slight localization of transforming growth factor beta 1 and fibronectin and inflammatory cell infiltration were observed in the interlobular space. Twelve to 24 h after duodenal ligation the infiltration of polymorphonuclear leukocytes and the deposition of transforming growth factor beta 1 and fibronectin were observed extensively in the interlobular and intralobular spaces. After release of the loop, infiltration of fibroblasts and marked deposition of fibronectin and type-III collagen were observed around the tubular complexes, but the deposition of transforming growth factor beta 1 was slight. Also, fibronectin and type-III collagen were shown to be produced by fibroblasts and acinar cells. CONCLUSIONS: These findings suggest that transforming growth factor beta 1 appears at the injured sites from the early stage of acute pancreatitis. Moreover, it is extensively related to the production of extracellular matrix such as fibronectin and type-III collagen. Furthermore, these substances are closely involved in the healing process of acute pancreatitis.

Acute Disease↗
PubMed1995

Leukocyte adhesion molecules in the liver and plasma cytokine levels in endotoxin-induced rat liver injury.

BACKGROUND: The interactions between polymorphonuclear neutrophils (PMNs) and sinusoidal endothelial cells (SECs) have been known to be involved in the pathogenesis of acute liver injury. It has been also reported that tumor necrosis factor-alpha (TNF-alpha) up-regulates ICAM-1 expression on SECs and that interleukin-8 (IL-8) provokes rapid activation of CD11/CD18 on PMNs. These findings expand into the relationship between the expression of leukocyte adhesion molecules (ICAM-1, CD11a/CD18 and CD11b/CD18) in liver tissues and plasma TNF and IL-8 levels after lipopolysaccharide (LPS)-induced liver injury in rats. METHODS: Male Wistar rats weighing 200-250 g were treated with 2 mg LPS/kg intravenously in a 0.2- to 0.25-ml volume. Liver and blood samples were obtained at 1, 3, 8, and 12 h after LPS exposure. Plasma TNF and IL-8 levels were measured using bioassay and specific enzyme-linked immunosorbent assay, respectively. Liver samples were fixed and studied by immunohistochemistry using specific monoclonal antibodies against ICAM-1, CD11a, and CD11b. RESULTS: The TNF level showed a peak at 1 h (23.3 +/- 11.4 IU/ml), and the IL-8 level showed a peak at 3 h (343.1 +/- 110.5 ng/ml) after LPS exposure. An increase in the number of PMNs in the liver was observed as early as 1 h and continued until 12 h after LPS exposure. PMNs adhered to degenerated SECs and hepatocytes. ICAM-1 on SECs was diffusely and strongly expressed at 8 h, and PMNs adhered to SECs expressed both CD11a and CD11b. ICAM-1 was also observed on hepatocytes. CONCLUSION: These data suggest that PMN-SEC and PMN-hepatocyte interactions via leukocyte adhesion molecules, related to inflammatory cytokines such as TNF and IL-8, exist and play an important role in the pathogenesis of acute liver injury.

Animals↗
PubMed1995

[Study on relationship between teeth clenching in intercuspal position and isometric movement of upper limbs].

To investigate the relationship between teeth clenching in intercuspal position and isometric shoulder adduction movement in 12 normal subjects, the muscle strength and electromyogram (EMG) of the seven muscles concerned were measured by using Cybex 6000 Extremity Testing and Rehabilitation System and surface EMG analyzing system. Normalized peak torque (n-PT) and normalized average torque (n-AT) were analyzed to find muscle strength. Normalized integrated EMG (n-iEMG) was analyzed to find muscle activity. The results were as follows: 1. For n-AT, the value in 100%MVC significantly increased by 5.4% as compared with that in the rest position (RP). 2. There was a significant positive correlation between biting force of intercuspal position (ICP), 20%MVC, 50%MVC and 100%MVC and muscle strength of n-PT and n-AT. 3. For n-iEMG, the value of latissimus dorsi, teres major, triceps brachii and forearm extensor muscles in 100%MVC significantly increased by 8.4%, 13.0%, 9.3% and 18.9% respectively as compared with that in RP. 4. There was a significant positive correlation between biting force of intercuspal position (ICP), 20%MVC, 50%MVC and 100%MVC and muscle activity of pectralis major, latissimus dorsi, teres major, biceps brachii, triceps brachii, forearm extensor and forearm flexor muscles.

Adult↗
PubMed1995

[The long-term results of isolated aortic valve replacement with 19 mm prostheses].

Twenty-five patients underwent isolated aortic valve replacement with 19 mm valves (8 cases with St. Jude Medical prostheses, 7 cases with Carpentier Edwards Pericardial prostheses and 10 cases with Björk-Shiley prostheses). Postoperative echocardiography revealed data as follows. Peak pressure gradients (mmHg) were 40.4 +/- 14.4 (mean +/- S.D.) in the SJM group, 23.4 +/- 9.8 in the CEP group and 50.5 +/- 16.7 in the BS group. End-diastolic left ventricular dimension in the BS group and interventricular septum thickness in the CEP group was reduced significantly. All but one case were in NYHA functional class I or II, and clinical improvement was almost satisfactory in all groups. However, in the BS group, 4 cases showed peak pressure gradients more than 50 mmHg. We conclude that 19 mm aortic prostheses in this study can provide satisfactory results. However, as for mechanical valve, it may be undesirable to choose BS prosthesis.

Adult↗
PubMed1995

A proposal mechanism of early delayed gastric emptying after pylorus preserving pancreatoduodenectomy.

To identify the cause of early delayed gastric emptying after pylorus preserving pancreatoduodenectomy (PPPD), we evaluated patients following PPPD and other upper gastrointestinal surgery. An acetaminophen assay was used to objectively evaluate gastric emptying. We observed transient delayed gastric emptying after PPPD in the early postoperative period. This was recognized not only in patients after PPPD but also in patients after standard pancreatoduodenectomy (STPD). The gastric emptying pattern was different between patients with partial gastrectomy and patients with STPD, despite similar reconstructions of gastroenterostomy (Billroth I type reconstruction). There was no early delayed gastric emptying in patients with Billroth II type reconstruction after PPPD or in those with preservation of the vagus nerves after cholecystectomy or transabdominal esophageal transection. We speculate that the anatomic position, namely, a transient torsion or angulation of the reconstructed alimentary tract is the main cause of early delayed gastric emptying after pancreatoduodenectomy.

Acetaminophen↗
PubMed1995

[A case of thromboexclusion for thoracic descending aortic pseudoaneurysm with Behçet's disease].

A 59-year-old woman with Behçet's disease was admitted because of hemoptysis. She had undergone resection and patch closure of a thoracic descending aortic aneurysm 6 years before this current admission. The aortography after the first operation had shown a pseudoaneurysm at the operative site. In the current operation, we performed thromboexclusion with a temporary occlusion balloon in the distal thoracic descending aorta above Adamkiewicz. The post operative course was uneventful. Postoperative angiography demonstrated exclusion of the pseudoaneurysm and the patient was discharged without recurrent hemoptysis.

Aneurysm, False↗
PubMed1995

[An autopsy care of hemophagocytic syndrome associated with miliary tuberculosis].

A 56-year-old female with chronic renal failure secondary to diabetic glomerulosclerosis was hospitalized and had a fluminant clinical course with pancytopenia, abnormal liver function tests, and coagulopathy. Bone marrow aspirate findings were typical of reactive hemophagocytosis. The patient died after 21 days of hospitalization and autopsy findings showed disseminated miliary tuberculosis. To our knowledge, only eight cases of miliary tuberculosis with bone marrow hemophagocytosis have previously been described. The presence of reactive hemophagocytosis requires a prompt and thorough search for treatable infections.

Female↗
PubMed1995

[Pseudoaneurysm of saphenous vein graft after CABG].

A 69-year-old man was admitted because of angina pectoris and thoracic descending aorta aneurysm. Staged operations were planned. First, he underwent CABG (coronary artery bypass grafting) with SVGs (saphenous vein grafts) to #4 PD, #7 and #12. Aprotinin was administrated to reduce blood loss. The routine postoperative graft angiography and enhanced CT showed a pseudoaneurysm in the SVG to #4 PD. We planned an elective operation of pseudoaneurysm repair and graft replacement of thoracic descending aorta. Also in this second operation, continuous infusion of aprotinin was started after the induction of anesthesia. About 30 minutes later, he suddenly fell in shock and cardiac arrest. Partial cardiopulmonary bypass was established and median sternotomy was performed. In the mediastinum, no bleeding was found. We found out a bleeding point of the SVG to #4 PD and a hemostatic clip on the right ventricule, and closed the bleeding point with suture. The cause of the pseudoaneurysm seemed to be defluxion of the hemostatic clip for a side brunch of the SVG. The cause of the preoperative shock may be an anaphylaxis to readministrated aprotinin.

Aged↗
PubMed1995

Correlation of in vivo infectivity of hepatitis C virus to in vitro infectivity and to virion properties.

Serum from an individual who was chronically infected with hepatitis C virus (HCV) was inoculated into chimpanzees and a human T cell line. The serum contained a quasispecies population of HCV consisting of at least six genetic sequences. However, only two sequences (the same two sequences) were recovered both in chimpanzees and in cultured human T cell line, indicating that infection of the culture system reflects infection in vivo.

Amino Acid Sequence↗
PubMed1994

Domain structures of the MS ring component protein (FliF) of the flagellar basal body of Salmonella typhimurium.

The proximal end of the flagellar basal body consists of a structure called the MS ring complex: two (M and S) rings with different thicknesses closely apposed and a rod extending from the center of the S ring. It has been shown that the MS ring complex consists of multiple copies of single protein FliF (molecular mass 61 kDa). We analyzed the domains of FliF to elucidate how a single protein can be used to construct a complicated particle with several distinct sub-structures. Tryptic digestion of the MS ring complex gave rise to a structure which lacked most of the M ring portion by electron microscopy and showed a major band at 25 kDa by SDS/gel electrophoresis. Amino acid sequence analysis of this band showed that both terminal regions of FliF have been digested, leaving a semi-stable peptide starting from Phe120 and ending at around 400. In addition, we constructed a truncated fliF gene which encodes a FliF lacking 103 amino acid residues from the C terminus. Amplification of the truncated FliF gave rise to a ring complex lacking the rim of the M ring. From these results we assign both terminal regions of FliF to the M ring. Possible domain structures of FliF corresponding to the S ring and the rod are also discussed.

Amino Acid Sequence↗
PubMed1994

Quantitative analyses of the structure-hydrophobicity relationship for N-acetyl di- and tripeptide amides.

The partition coefficient (P) of neutral species and the apparent partition ratio (P') at pH 7 of the ionized form were measured with the 1-octanol/water system for a number of N-acetyl di- and tripeptide amides having un-ionizable and ionizable side chains. Their log values were studied in terms of free-energy-related substituent and substructural parameters using regression analysis to give correlation equations of high quality physicochemically as well as statistically. The intrinsic hydrophobicity of side-chain substituents and their steric effect on the relative solvation of the backbone CONH groups were significant in determining the log P values of the un-ionizable acetyl peptide amides. For the log P value of peptides with polar side-chain substituents, respective indicator variable terms were required to account for the sum of specific effects of substituents such as intramolecular hydrogen-bond formation and the "polar proximity factor" for augmentation of the hydrophobicity. For the log P'(pH 7) value of basic and acidic peptides, the ability of counterionic species to form ion-pairs, the change in the apparent hydrophobicity of ionizable groups from the intrinsic value for their nonionized forms, the effect of ion-pairing itself, and other effects were additionally considered. From the regression coefficients of the parameter terms in correlation equations an effective hydrophobicity index was defined for each side chain, and the application and its limitation were suggested.

Acetylation↗
PubMed1994

Quantitative structure-hydrophobicity and structure-activity relationships of antibacterial gramicidin S analogs.

The structure-hydrophobicity-antibacterial activity relationships of gramicidin S and its analogs retaining a beta-pleated sheet structure were examined quantitatively with physicochemical substituent and molecular parameters using regression analyses. Variations in their apparent hydrophobicity in an octanol/buffer (pH 7) system, log P' (O/W), were analyzed in terms of the "effective" hydrophobicity and steric parameters of side chain substituents of residues at certain positions in the molecule; however, some of the conformational factors have not been fully defined. For the partitioning into liposomes and the growth inhibitory activity against species of Gram-positive bacteria, the log P' (O/W) value simulated the hydrophobic effects of gramicidin S and its analogs better than substituent parameters. The side chain hydrophobicity was assumed to work together with effects attributed to variations in the entire cyclic peptide structure including conformational components undefined in the structure-log P' (O/W) analysis in these activities.

Amino Acid Sequence↗
PubMed1994

What roles does the organum vasculosum laminae terminalis play in fever in rabbits?

Experiments were designed to clarify the role of the brain's organum vasculosum laminae terminalis (OVLT) in the development of fever in rabbits. Rectal and ear skin temperatures were recorded in conscious animals in which the OVLT had been electrolytically destroyed or in which the preoptic anterior hypothalamus (PO/AH) had been transected bilaterally. When the OVLT had been ablated the febrile responses to intravenous injection of interleukin-1 beta (IL-1 beta) or tumour necrosis factor alpha were significantly attenuated, while those to intracerebroventricular injection of IL-1 beta were not affected. Fever induced by intracerebroventricular injection of prostaglandin E2 (PGE2) was prolonged significantly. The febrile responses to intravenous injection of IL-1 beta and to intracerebroventricular injection of PGE2 were attenuated when the transection was located caudally to the anterior wall of the third ventricle and extended laterally more than about 3 mm in the ventricular wall. The results show that the OVLT region is a site through which signals to increase body temperature are transferred from the blood to the brain in rabbits.

Animals↗
PubMed1994

Neutrophil activation after percutaneous transluminal coronary angioplasty.

We investigated whether percutaneous transluminal coronary angioplasty (PTCA) would induce neutrophil activation in patients with coronary artery disease. Blood samples were taken from the coronary sinus in 14 patients who underwent PTCA and in 9 control subjects who underwent coronary arteriography (CAG). Flow cytometry was used to measure membrane surface expression of beta 2 integrin (CD11b) and the generation of hydrogen peroxide in neutrophils after ex vivo phorbol myristate acetate stimulation by 2,'7'-dichlorofluorescein. Neutrophil elastase was measured by an immunoenzymatic method. Surface expression of CD11b increased significantly, approximately twofold, after PTCA but not after CAG. Mean fluorescence intensity of 2',7'-dichlorofluorescein in stimulated neutrophils decreased significantly after PTCA, suggesting a previous in vivo activation, but not after CAG. Neutrophil elastase increased significantly after PTCA but not after CAG. These data indicate that PTCA induces neutrophil activation and suggest that neutrophils may contribute to the ischemic injury.

Adult↗
PubMed1994

Spinal cord protection: development of a paraplegia-preventive solution.

We present a clinically available method to protect the spinal cord against ischemic or reperfusion injury and to prevent paraplegia after cross-clamping of the aorta. We separated 35 rabbits into five equal groups and clamped each animal's abdominal aorta distal to the left renal artery. We also occluded the aortas 2 cm above the iliac bifurcation for 45 minutes with inflated 5F balloon catheters. Through the catheter port distal to each balloon one of four different solutions was infused at 3 degrees C for 3 minutes at a rate of 5 mL/min (group I, uninfused control; group II, lactated Ringer's solution; group III, lactated Ringer's solution + 30 mg/kg of methylprednisolone; group IV, lactated Ringer's solution+methylprednisolone + 3 mL of 20% mannitol; group V, lactated Ringer's solution+methylprednisolone+mannitol + 10 mg/kg of vitamins E and C). We assessed the neurologic status of the hind limbs on the second postoperative day using Tarlov's criteria. The neurologic status in groups III, IV, and V was significantly superior to that of group I (p < 0.05, groups III versus I; p < 0.01, groups IV and V versus I). Spastic paraplegia occurred in 71% of group I, in 43% of group II, in 29% of group III, in 14% of group IV, and not at all in group V. The infusion of our specially blended solution with several spinal cord neuroprotective properties (hypothermia, methylprednisolone, mannitol, and vitamins E and C) achieved the best spinal cord protection against ischemic or reperfusion injury and prevented postoperative paraplegia.

Animals↗