Search PubMed⌕ Search

Biomedical subjects

T Tsunoda

Publications and source records attributed to T Tsunoda.

At least 145 records · Page 8Linked to original sources
PubMed1994

Spontaneous pancreatitis in spontaneously hypertensive rats.

The pancreatic lesions in 6- to 36-week-old spontaneously hypertensive rats (SHR), stroke prone SHR (SHRSP) and Wistar-Kyoto rats (WKY) were examined histopathologically. Inflammatory cell infiltration with hemorrhage and stromal fibrosis became evident in 12-week-old SHR and SHRSP together with acinar atrophy and/or degeneration and ductular proliferation. These changes in SHR and SHRSP were even more prominent at the age of 24 weeks and extremely severe at 36 weeks. In addition, in SHR and SHRSP over 12 weeks of age, small necrosis of acinar cells was found occasionally together with fibrosis and arteriosclerosis. Pancreatic arteriosclerosis was marked in SHR and SHRSP over 24 weeks of age at the level of arterioles < 200 microns in diameter. Adrenergic nerve fibers stained by fluorescence histochemistry were present around the pancreatic arteries and ducts and within the parenchyma, and they were denser in SHR and SHRSP than in WKY, indicating hyperinnervation of the sympathetic nervous system in SHR and SHRSP. It is suggested that the pancreatic ischemia caused by arteriosclerosis due to facilitation of the sympathetic nervous system is an important factor in the pathogenesis of the spontaneous pancreatitis of SHR and SHRSP.

Animals↗
PubMed1994

Induction of extrahepatic biliary carcinoma by N-nitrosobis(2-oxopropyl)amine in hamsters given cholecystoduodenostomy with dissection of the common duct.

The methods we used to produce a carcinoma in the extrahepatic bile duct and gallbladder in hamsters are described along with the characteristics of the induced tumors. Female Syrian golden hamsters were first subjected to cholecystoduodenostomy with dissection of the extrahepatic bile duct on the distal end of the common duct (CDDB) and were, 4 weeks later, treated with weekly subcutaneous injections of N-nitrosobis(2-oxopropyl)amine (BOP) at a dose of 10 mg/kg body weight for 9 weeks. The animals were killed at the 12th, 16th and 20th week after the initiation of BOP treatment. Extrahepatic bile duct carcinoma developed in 16%, 24% and 41% and gallbladder carcinoma occurred in 58%, 81% and 82% of the hamsters, respectively, at the corresponding times of killing. The incidences were significantly higher than those in sham-operated controls (P < 0.01). The induced extrahepatic bile duct carcinomas were predominantly of the polypoid type and gallbladder carcinomas were of the papillary type in growth form, being morphologically similar to early stage biliary carcinoma in humans. Immunohistochemical staining using bromodeoxyuridine and anti-bromodeoxyuridine monoclonal antibody demonstrated that the CDDB procedure greatly accelerated the cell kinetic activity of the biliary epithelium, and this was considered to be a major factor promoting the development of biliary carcinomas in this hamster model. In conclusion, this new model provides a high incidence of tumor development at the extrahepatic biliary tract and is expected to be useful for clarifying the characteristics of this highly malignant tumor.

Animals↗
PubMed1994

[Basic and clinical studies of fleroxacin on infectious enteritis. Research Group of AM-833 on infectious enteritis].

A clinical study was conducted on fleroxacin (FLRX) in 143 patients and carriers with infectious enteritis (shigellosis, Salmonella enteritis, Campylobacter enteritis, pathogenic Escherichia coli enteritis, Vibrio parahaemolyticus enteritis, cholera, multiple bacterial infections, pathogen-negative enteritis). Furthermore, its antibacterial activity against clinical isolates, fecal concentration and effect on fecal microflora were conducted. FLRX was administered orally in doses of 200 mg once a day (200 mg group) or 300 mg once a day (300 mg group) for 3 days to cholera, for 7 days to Salmonella enteritis and for 5 days to the other infectious enteritis. The clinical efficacy rates were 100% in both the 200 mg and 300 mg groups. The bacteriological efficacy rates were 100% against Shigella spp., Salmonella spp., pathogenic E. coli, V. parahaemolyticus and V. cholerae O1, and 63.6% against Campylobacter spp. in the 200 mg group. The rates of the 300 mg group were 93.3% against Shigella spp., and 100% against Campylobacter spp. and pathogenic E. coli. As adverse effects, skin rash was observed in 1 case each in both groups (1.1%, 2.1%). Abnormal laboratory findings consisted of 1 case of increased eosinophils and 1 case of elevated GOT and GPT levels in the 200 mg group (2.8%), and 1 case of elevated GPT in the 300 mg group (2.9%). The clinical usefulness rates were 92.9% and 93.3% in the 200 mg and 300 mg groups, respectively. Antibacterial activity was somewhat inferior to that fo ciprofloxacin and equal to or better than that of norfloxacin, demonstrating MIC90 values against Shigella spp., Salmonella spp., pathogenic E. coli, V. parahaemolyticus and Campylobacter spp. of 0.1, 0.2, 0.1, 0.2 and 0.78 micrograms/ml, respectively. Peak fecal concentrations of the drug were 49.0 micrograms/g and 274.4 micrograms/g in the 200 mg group, and 43.3 micrograms/g and below the detection limit (5.0 micrograms/g) in the 300 mg group. With respect to fecal microflora (4 cases), a decrease in Enterobacteriaceae was observed in 3 cases during dosing. But this change showed a tendency to recover after completion of dosing. No effects were observed on anaerobic bacteria.

Adolescent↗
PubMed1994

Malignant transformation of benign hidroacanthoma simplex.

A case of malignant hidroacanthoma simplex of the anterior aspect of the right ankle in a 75-year-old man is reported. A specimen obtained in 1987 showed the features of benign hidroacanthoma simplex, whereas that taken in 1991 revealed malignant transformation. We performed immunohistochemical studies on these two specimens, and they suggest this tumor derives from the outer cells of intraepidermal ducts. The proliferating cell nuclear antigen labelling index and argyrophil nucleolar organizer regions of these two specimens were also compared and correlated with malignant transformation.

Aged↗
PubMed1994

Oral mucosa malignant melanoma in situ with involvement of the perioral skin.

A 66-year-old Japanese woman had a pigmented macule spreading from the internal aspect of the cheek to the vermilion border and perioral skin. Histology was that of in situ malignant melanoma. This case appears to be unique as it implies a longer period of radial growth than is usually observed in malignant melanoma of the oral mucosa.

Aged↗
PubMed1994

[A 10-year field surveillance in Hinohara Village of Tokyo Prefecture from 1981 to 1990].

We conducted a surveillance to clarify the relationship between risk factors for diseases of adulthood and lifestyle in a Japanese rural community, Hinohara Village, a small village outside of Tokyo. The survey, carried out from 1981 to 1990 among residents aged 40 and over, comprised physical examination and blood chemistry with a questionnaire about dietary intake. Mean systolic blood pressure significantly decreased (p < 0.0001) from 140.9 mmHg in 1981 to 132.3 mmHg in 1990, whereas mean serum total cholesterol, mainly of male examinees, increased (p < 0.0001) from 181.4 mg/dl in 1981 to 191.7 mg/dl in 1990. Dietary salt intake significantly decreased (p < 0.0001) from 14.3 g/day in 1981 to 12.1 g/day in 1990. Adjusted mortality rate per 1,000 residents from cerebrovascular disease in this village decreased from 1.80 in 1981 to 0.50 in 1990. In contrast to its decline, the mortality rates from heart disease, bronchitis/pneumonia and neoplasms were 0.40, 0.35 and 0.55 in 1981 and increased to 1.25, 1.10 and 0.64 in 1990. The prevailing practice of maintaining a low-salt diet might cause the decrease of systolic blood pressure, which in turn was thought to decrease the mortality rate from cerebrovascular diseases. Although our previous study before 1981 suggested that total cholesterol was one of the preventive factors against cerebrovascular disease, in the present study a preventive effect of cholesterol was not substantiated. In contrast, cholesterol is a possible risk factor for ischemic heart disease. Thus, a changing pattern of risk factors of diseases of adulthood was observed in this village.

Adult↗
PubMed1994

Tap needle for laparoscopic surgery: a safe and easy technique for holding tissues.

To achieve and maintain a wide operative field during laparoscopic surgery, we developed a "tap needle" that can penetrate the skin into the peritoneal or pleural cavities and can be used to pick up threads introduced into the cavities to elevate tissues or organs. The characteristics of the tap needle and method of use are reported herein.

Abdomen↗
PubMed1994

Small bowel tumors: an analysis of tumor-like lesions, benign and malignant neoplasms.

The clinical records of 72 patients with 73 small bowel tumors diagnosed over a period of 25 years were reviewed. The tumors consisted of 23 tumor-like lesions, 17 benign neoplasms and 33 malignant neoplasms, the mean size of which were 2.1 cm, 5.2 cm and 7.7 cm in diameter, respectively. The tumor-like lesions and benign neoplasms were found even in children, while the malignant neoplasms were observed in patients 43 years old or over. The most common tumor-like lesion was aberrant pancreatic tissue. The common benign neoplasms were leiomyoma, lipoma and adenoma. The malignant neoplasms consisted of adenocarcinoma, malignant lymphoma and leiomyosarcoma. Regardless of tumor type or tumor location, the lesions 4 cm or larger often caused abdominal symptoms. Most of the lesions less than 4 cm caused no symptoms, but the malignant lesions of this size in the ileum elicited abdominal symptoms in all patients. The presence of lesions was diagnosed preoperatively in 26.1% of patients with tumor-like lesions, 47.1% with benign neoplasms and 48.5% with malignant neoplasms. Resection was performed in all but one patient with tumor-like lesions and benign neoplasms. Among malignant lesions, the resectability rate was 80.0% for adenocarcinoma and 100% for both malignant lymphoma and leiomyosarcoma. However, the patients with malignant lesions showed a poor prognosis. In conclusion, it is important to recognize small bowel tumors--especially malignant lesions--as a rare but possible cause of abdominal symptoms and thereby to contribute to the early detection and successful management of small bowel tumors.

Adenocarcinoma↗
PubMed1994

Biliary diverticulum with pancreaticobiliary maljunction.

The case of a 48-year-old Japanese man with a biliary diverticulum (type B by the so-called Alonso-Lej classification) accompanied by pancreaticobiliary maljunction is reported on herein. The patient had epigastric distress, and an ultrasound examination suggested gallstones and adenomyomatosis of the gallbladder. Subsequent endoscopic retrograde cholangiopancreaticography demonstrated a 3 mm biliary diverticulum in the intrahepatic bile duct and pancreaticobiliary maljunction. Intra-operative cholangiography confirmed both the diverticulum and the pancreatiocobiliary maljunction. Cholecystectomy alone was performed. The histological diagnosis of the gallbladder was adenomyomatosis with intramural calculi. Except for continuing mild hyperamylasemia, the patient has been doing well for 18 months following surgery. To our knowledge, only two cases, of type B bile duct dilatation with pancreaticobiliary maljunction, including ours, have been reported in the English and Japanese literature. The size of the diverticulum in our case was smaller than in the other reported case. The relationship between pancreaticobiliary maljunction and type B bile duct dilatation is discussed.

Adenomyoma↗
PubMed1993

Sclerosing encapsulating peritonitis combined with peritoneal encapsulation.

The combined occurrence of idiopathic sclerosing encapsulating peritonitis and peritoneal encapsulation is described. A 52-year-old man presented with intestinal obstruction. The results of preoperative examinations were suggestive of sclerosing encapsulating peritonitis. Laparotomy revealed the concurrence of peritoneal encapsulation and sclerosing encapsulating peritonitis. The thick membrane of sclerosing encapsulating peritonitis was freed with multiple incisions. After operation, the patient reverted to the preoperative state. The condition, however, was alleviated with conservative therapy consisting of intravenous hyperalimentation and nasogastric suction. To our knowledge, the combined occurrence of sclerosing encapsulating peritonitis and peritoneal encapsulation has never before been reported.

Collagen↗
PubMed1993

Expression of carcinoembryonic antigen in fresh human gastric cancer cells assessed by flow cytometry.

The expression of carcinoembryonic antigen (CEA) by tumor cells from freshly excised human gastric cancers was investigated using flow cytometry (FCM). Highly purified fresh human cancer cells were obtained from solid tumors in 20 patients and from malignant ascites in 6 patients. Thirteen of the 26 tumors were positive for CEA by FCM. CEA expression was more common in well-differentiated tumors than in poorly differentiated tumors. CEA expression was investigated by both FCM and immunohistochemistry in 9 patients, and the two methods agreed in 8 of them. However, quantitative evaluation of CEA expression could only be performed by FCM and not by immunohistochemical staining. FCM could analyze the expression of CEA not only on the cell membrane but also in the cytoplasm, by using gastric cancer cells with or without Triton X-100 treatment. Thus, this study showed that CEA expression can be determined and evaluated quantitatively by FCM.

Aged↗
PubMed1993

Extended right lobectomy for Caroli's disease: report of a case and review of hepatectomized cases in Japan.

A 27-year-old man was admitted to Nagasaki University Hospital complaining of fever. Percutaneous transhepatic cholangiography revealed multiple cystic dilatations of the intrahepatic bile ducts of the right lobe, as well as three obvious cystic dilatations and several small dilatations of the intrahepatic bile ducts of the left lobe. An extended right lobectomy was performed. However, the patient later suffered from repeated cholangitis and required the administration of antibiotics as a result of remnant cystic dilatations and stricture of the extrahepatic bile duct following hepatectomy. A slight enlargement of the remnant cystic dilatations, but no new cystic dilatations, were also recognized in the regenerating liver. We reviewed nine cases of hepatectomy for Caroli's disease reported in the Japanese literature, as well as our own case. Among these, four of the seven patients subjected to hepatic resection involving unilateral lobe demonstrated a good recovery. On the other hand, it is difficult to successfully treat Caroli's disease involving both lobes of the liver by hepatic resection.

Adult↗
PubMed1993

A case of intracystic carcinoma of the breast: the importance of measuring carcinoembryonic antigen in aspirated cystic fluid.

We report a case of intracystic carcinoma of the breast in which a correct preoperative diagnosis was achieved on the basis of an increased level of carcinoembryonic antigen (CEA) in the aspirated cystic fluid. A 62-year-old woman was admitted with a 10 x 10 cm painful mass occupying the right breast. Ultrasonography revealed a cystic lesion with papillary projections arising from the cyst wall. Cytological examinations showed no malignant cells in the hemorrhagic aspirated fluid. However, the CEA level in the fluid was 5.5 times higher than the serum CEA level using a murine anti-CEA monoclonal antibody. The high level of CEA led to the preoperative diagnosis of intracystic carcinoma. Histological examinations confirmed the diagnosis of intracystic carcinoma after a modified radical mastectomy. Immunohistochemical staining by an anti-CEA monoclonal antibody elicited a strong positivity with diffuse intracytoplasmic distribution in the carcinoma cells, although heterogeneity of staining was observed. It is suggested that the measurement of the CEA value by anti-CEA monoclonal antibody in the aspirated fluid is easy, safe, and helpful for the definitive diagnosis of intracystic carcinoma of the breast.

Breast Neoplasms↗
PubMed1993

Establishment of a transplantable carcinoma arising from the intrahepatic bile duct in Syrian golden hamsters.

A subcutaneously transplantable cancer line from the intrahepatic bile duct (IHBD) induced by N-nitrosobis(2-oxopropyl) amine was established in Syrian golden hamsters. The doubling time of this tumour was 2.6 days when 2 x 10(5) tumour cells were inoculated subcutaneously (take-up rate was 100%). Growth of the tumour was significantly faster in male hamsters but neither oestrogen nor androgen receptors were detected in the tumour. The primary and all allograft tumours were tubular adenocarcinomas with fibrosis and a scirrhous pattern resembling human IHBD carcinoma of the peripheral type. Transmission electron microscopic findings showed irregular glands covered with numerous microvilli. Blood-group-related antigens including A, B and H were positive. P-Glycoprotein, which is an indicator of multidrug resistance, was also positive. Carcinoembryonic antigen and CA19-9 as general tumour markers of the biliary tract were negative. The deoxyribonucleic acid (DNA) pattern of this transplantable carcinoma was diploid. This newly established animal model of a transplantable IHBD carcinoma can be used to examine the mechanisms of synthesis and secretion of tumour-associated antigens and to study potential therapeutic agents.

Adenocarcinoma↗
PubMed1993

Hyperbaric oxygen therapy in the treatment of refractory peripancreatic abscess associated with severe acute pancreatitis.

Five patients with peripancreatic abscesses associated with severe acute pancreatitis were treated by hyperbaric oxygen therapy (HBO). In 3 patients, the course after surgical mobilization of the pancreas and drainage of the pancreas bed was complicated by peripancreatic abscesses. HBO was conducted under a pressure of 2.8 atmospheres for two hours daily. Four of the 5 patients showed a progressive improvement in their condition. In one patient who failed to respond despite seven sessions of HBO, Pseudomonas aeruginosa was isolated from the discharge, and resection of necrotic tissue and drainage were performed. The main effects of HBO were the alleviation of high spiking fever, the improvement of white blood cell count and serum amylase levels, and the reduction of the abscess size. We recognized HBO to be a successful treatment for peripancreatic abscess associated with severe acute pancreatitis and better results were obtained than in cases that did not receive HBO.

Abscess↗
PubMed1993

Solid and cystic tumor of the pancreas occurring without cyst formation in an adult male.

A solid and cystic tumor (SCT) of the pancreas occurring in a 35-yr-old male is reported. Cut sections of the specimen revealed a solid, ill-defined mass measuring 2.5 x 2.3 x 2.0 cm, without cystic or necrotic changes. Histologically, the solid tumor consisted of small, round acidophilic cells invading the surrounding pancreatic parenchyma. The tumor cells were positive for alpha-1-antitrypsin and neuron-specific-enolase. Ultrastructural studies revealed clear nuclei with no zymogen, but immature secretory granules in the cytoplasm of the tumor cells, which had a junctional complex-like structure. These findings were consistent with the so-called solid and cystic tumor of the pancreas. There was neither a capsule surrounding the tumor nor a papillary structure, known to be characteristic findings of the SCT tumor. The small tumor reported in the present article might represent an early-stage SCT of the pancreas.

Adult↗