Search PubMed⌕ Search

Biomedical subjects

T Tsuji

Publications and source records attributed to T Tsuji.

At least 1,099 records · Page 61Linked to original sources
PubMed1982

Detection of serum albumin receptor in hepatitis B virus carriers by enzyme-linked immunosorbent assay.

The relationship between glutaraldehyde-treated polymeryzed human serum albumin (pHSA) and HBe antigen (HBeAg)-positive serum was examined by the use of a new enzyme-linked immunosorbent assay (ELISA). The author succeeded in measuring the pHSA binding activity (pHSA-BA) of HB surface antigen (HBsAg) particles in the present ELISA method using horseradish peroxidase-labelled pHSA after fixation of HBsAg on an anti-HBs-coated well of polystyrene microplates. In HBeAg-positive group, the pHSA-BA of sera of 40 asymptomatic carriers and 2 chronic persistent hepatitis (CPH) patients were higher than those of 8 chronic active hepatitis (CAH) (p less than 0.01) and 8 liver cirrhosis sera (p less than 0.05). On the contrary, in the anti-HBe-positive group the pHSA-BA of 17 asymptomatic carriers and 3 CPH sera were lower than those of 8 CAH (p less than 0.005) and 10 liver cirrhosis patient sera (p less than 0.005). In the both-negative group the pHSA-BA of 8 asymptomatic carrier and 3 CPH sera were also lower than that of 8 CAH (p less than 0.05). In acute exacerbation of HBsAg-positive CAH the pHSA-BA elevated one to two months before the peak of S-GPT level, being correlated with the DNA-polymerase activity. Because of its apparent reproducibility, it is concluded that low cost and some advantages may have clinical utility in the same setting as the HBeAg is now used.

Carrier State↗
PubMed1982

Secondary localized cutaneous amyloidosis in solar elastosis.

A case of secondary localized cutaneous amyloidosis in solar elastosis was studies by light and electron microscopy. Amyloid deposition was restricted to areas with elastotic changes, and in some areas both changes were intermingled with each other. The amyloid was permanganate-sensitive, and proved to be protein AA. Ultrastructurally it was composed of fine tubular filaments. It is suggested that the amyloid deposition in this case was related to sunlight damage of the skin.

Aged↗
PubMed1982

Scanning electron microscopy of dermal elastic fibres in transverse section.

The fibrous networks of dermal elastic fibres have been studied in transverse section using a scanning electron microscope. In the uppermost dermis the elastic fibres have no free endings, but form a reticulate appearance by connecting with each other. In the mid-dermis the fibres run parallel to the epidermal surface, aligning in the same direction. In the lower dermis many of the fibres are organized into broad thin sheets with numerous branches.

Elastic Tissue↗
PubMed1982

Focal dermal hypoplasia syndrome. An electron microscopical study of the skin lesions.

An 11-month-old female with focal dermal hypoplasia syndrome is described. Histologically, the skin lesions were characterized by a marked decrease in dermal connective tissue. Ultrastructurally, there were a number of fine filamentous structures measuring 5 to 70 nm within and between the bundles of collagen; most of the dermal fibroblasts of the lesions possessed no remarkably developed rough endoplasmic reticulum and Golgi complex. Multilocular fat cells which are regarded as young fat cells were often seen. It is suggested that this disease may be characterized by a decreased rate of collagen synthesis by fibroblasts and an overgrowth of adipose tissue.

Adipose Tissue↗
PubMed1982

Molecular heterogeneity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

The heat-labile enterotoxins produced by human enterotoxigenic Escherichia coli (LTh) and porcine enterotoxigenic E. coli (LTp) were purified to homogeneity, and their molecular properties were compared with those of purified cholera enterotoxin (CT). On polyacrylamide gel disk electrophoresis without sodium dodecyl sulfate, LTh, LTp, and CT differed in mobility, suggesting differences in their ionic charges. The pI values of LTh, LTp, and CT were 7.50, 8.10, and 6.80, respectively. On sodium dodecyl sulfate-polyacrylamide gel slab electrophoresis, the B subunit and A1 and A2 fragments of LTh, LTp, and CT differed in mobility, suggesting that they differed in molecular size. Their molecular sizes seemed to decrease in the following order: B subunit, LTh greater than LTp congruent to CT; A1 fragment, LTp greater than LTh congruent to CT; A2 fragment, LTh congruent to CT greater than LTp. Amino acid compositions of LTh, LTp, and CT were also compared.

Amino Acids↗
PubMed1982

Therapeutic effects of tiopronin on chronic hepatitis: a double-blind clinical study.

The efficacy of tiopronin (2-mercaptopropionyl glycine) 600 mg daily in the treatment of chronic active or chronic persistent hepatitis has been assessed in a double-blind controlled clinical trial of 12 weeks involving 165 Japanese patients with histologically proven disease. Treatment with the drug was associated with a significant improvement in abnormalities of serum transaminase and gamma glutamyl transpeptidase, with reversion towards baseline values on stopping the drug. Improvement was independent of the histological classification of the disease, or HBsAg status. The drug was well tolerated with few side-effects. The results of this short-term study indicate that tiopronin may be of value in the treatment of chronic hepatitis.

Acyltransferases↗
PubMed1982

Detection and characterization of circulating immune complexes during acute exacerbation of chronic viral hepatitis.

For detection and characterization of circulating immune complexes (CIC) in various liver diseases, a C1q binding test (C1q BT) was used. While the CIC level was almost normal in HB surface antigen (HBsAg) positive asymptomatic carriers, it was relatively high in patients with liver diseases. The study of the sedimentation rate of CIC in various liver diseases showed two kinds of CIC; one greater than the other. During acute exacerbation of chronic active liver diseases, the CIC level reached its peak 1-5 weeks before and after the peak of sGPT. In acid buffer, CIC in one patient with HBsAg positive severe chronic aggressive hepatitis was dissociated into 5-6 fractions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The two of these fractions proved to be HBsAg and IgG and another three or four undetermined components. At the acute exacerbation period of this case, the fraction pattern of CIC by SDS-PAGE was similar to each other at any of the four stages and HBsAg always composed one of the antigens.

Acute Disease↗
PubMed1982

[Concentration of cefotiam in the cerebrospinal fluid].

Cefotiam of 1 to 2 g was intravenously given during 15 to 60 minutes in 10 cases, and blood levels and cerebrospinal fluid (CSF) levels were studied. Following the drip infusion of cefotiam, maximum blood levels of 25.2 to 305 micrograms per ml was an average of 101.2 micrograms per ml were achieved at 15 to 60 minutes in 9 cases. Half life of cefotiam in serum was from 20 to 50 minutes, and mean time was 39.4 minutes. In contrast, maximum CSF levels of cefotiam were ranged 1.4 to 17.2 micrograms per ml and mean value was 5.7 micrograms per ml in 8 cases. The ratios of CSF levels to blood levels were calculated from 1.7 to 6.6% with an average of 4.6%. The CSF levels of cefotiam showed long delay and long decay. The period between the drip infusion and peak levels of cefotiam in CSF showed 60 to 420 minutes and mean time was 194 minutes. Half life varied between 45 and 270 minutes with an average of 178 minutes. No side effects were found in all cases.

Adult↗
PubMed1982

Early and late postoperative studies in coronary arterial lesions resulting from Kawasaki's disease in children.

From October, 1976, to February, 1980, eight children with coronary arterial lesions resulting from Kawasaki's disease underwent surgical treatment--aorta-coronary bypass, aorta-coronary bypass combined with right coronary aneurysmectomy, and aorta-coronary bypass combined with left ventricular aneurysmectomy. The autogenous saphenous vein was used for the bypass grafts. Selective angiography preformed 1 month after the operation revealed an early patency rate of 85%. Seven patients are now leading a normal school life, but one patient died suddenly during relatively strenuous exercise 3 years after the operation. Angiography performed in five patients 1 to 3 years after the operation and autopsy examination in one decreased patient revealed the late patency rate to be 56%. The relatively higher attrition rate of the grafts in the later stage might be ascribed to the smaller caliber of the coronary artery and saphenous vein, to possible inflammatory changes in both the graft and coronary arteries, or to unknown metabolic differences between children and adults.

Adolescent↗
PubMed1982

Effects of Mepivacaine on adrenergic neuroeffector junction of the isolated rabbit aorta.

The effect of mepivacaine on adrenergic neuroeffector junction was studied in the isolated rabbit aorta. Mepivacaine, 5 X 10(-5) to 5 X 10(-4) M, attenuated the contractile response to transmural neural stimulation, the attenuation being greater in the response at high frequency stimulations. The attenuation of the responses by mepivacaine was not prevented by prior application of cocaine. The concentration-response curve for norepinephrine was shifted to the right by mepivacaine, 5 X 10(-5) to 2 X 10(-3) M. The attenuation of the response to transmural stimulation was greater than that of the response to an equipotent concentration of exogenous norepinephrine. Pretreatment with mepivacaine, 5 X 10(-5) to 2 X 10(-3) M, protected alpha-adrenoceptors from persistent blockade by phenoxybenzamine in a dose-dependent manner. The contractile response to histamine was not significantly altered by mepivacaine in concentrations up to 5 X 10(-4) M. Mepivacaine, 5 X 10(-4) and 2 X 10(-3) M, decreased the response to high concentrations of KCl. Ca2+-induced contractions in aortic strips previously exposed to Ca2+-free media and depolarized by excess K+ were significantly inhibited by mepivacaine, 5 X 10(-4) and 2 X 10(-3) M. It may be concluded that mepivacaine causes vasodilation through an alpha-adrenoceptor antagonism in addition to a sympathetic nerve conduction blockade. High concentrations of mepivacaine appear to interfere with the transmembrane influx of calcium in the vascular smooth muscle.

Animals↗