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Biomedical subjects

T Tsuji

Publications and source records attributed to T Tsuji.

At least 1,063 records · Page 59Linked to original sources
PubMed1984

[Trigeminal neuralgia caused by arteriovenous malformation of the cerebellopontine angle--report of two cases].

Two cases of trigeminal neuralgia caused by arteriovenous malformation (AVM) located in cerebellopontine angle (CP angle) are presented. A 61-year-old woman has been suffered from the left 2nd and 3rd division trigeminal neuralgia, and gasserian ganglion rhizotomy did not relieve the pain. Ten days before admission the patient suddenly got right hemiparesis and left cerebellar ataxia. Bleeding of the left CP angle was noticed by computerized tomography (CT) scan and angiogram revealed AVM of the left CP angle. A 48-year-old man had right facial neuralgia for 4 years along and he received percutaneous retrogasserian glycerol rhizotomy twice, but the pain was not aleviated. Superior petrosal sinus was enhanced on conventional CT scan, and air contrast CT cisternography disclosed network shaped structure at the CP angle, which was revealed as AVM by vertebral angiography. The patient was completely relieved from the neuralgia after removal of the AVM. AVM of the CP angle that causes the trigeminal neuralgia is rare and the gasserian ganglion rhizotomy is little effective in aleviating the pain. Bleeding from AVM causes severe neurological deficits. Removal of AVM is important not only for pain relief but also for protecting bleeding. Air contrast CT cisternography is one of the safety and convenient methods to detect the AVM and other vascular anormalies resulting in trigeminal neuralgia.

Cerebellopontine Angle↗
PubMed1983

Structural requirements for the binding of high-mannose-type glycopeptides to immobilized pokeweed Pa-2 lectin.

The structural requirements for the interaction of asparagine-linked glycopeptides with immobilized pokeweed mitogen Pa-2 were investigated. Some high-mannose-type glycopeptides obtained from porcine thyroglobulin were found to have strong affinity for Pa-2-Sepharose, whereas complex- and hybrid-type glycopeptides were shown to have much weaker affinity. The elution profiles of various glycopeptides modified by glycosidase treatment and acetolysis showed that the total structure alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 2)-alpha-D-Manp-(1 leads to 3)-beta-D-Manp-(1 leads to 4)-beta-GlcpNAc-(1 leads to 4)-beta-GlcpNAc leads to Asn was essential for the binding of glycopeptides to a Pa-2-Sepharose column.

Animals↗
PubMed1983

The carbohydrate moiety of human platelet glycocalicin.

Glycocalicin, a predominant glycoprotein on the human platelet surface, has been purified from a platelet suspension by sonication, ammonium sulfate precipitation and acid treatment followed by chromatography on columns of wheat germ agglutinin-Sepharose and Sephacryl S-300. Ser/Thr-linked (O-linked) oligosaccharides were released by alkaline borohydride treatment, and fractionated by high performance liquid chromatography with an anion-exchange resin. The structure of a major oligosaccharide alditol separated by high performance liquid chromatography was investigated by a combination of compositional analyses, methylation and glycosidase treatments, and proposed to be a hexasaccharide alditol, NeuAc alpha 2-3Gal beta 1-4GlcNAc beta 1-6(NeuAc alpha 2-3Gal beta 1-3) N-acetylgalactosaminitol. We also found some sugar units which appeared to be intermediates in the biosynthetic pathway of the major hexasaccharide.

Blood Platelets↗
PubMed1983

Histochemical studies on the regeneration of aminergic nerves in rat cerebral artery after superior cervical ganglionectomy.

The course of regeneration of aminergic nerves in rat cerebral arteries was studied by means of histochemical methods, after uni- or bilateral cervical sympathectomy. Degeneration of aminergic nerves started on day 1 and was complete between days 3 and 7 after surgery. Between weeks 4 and 6, regenerating nerves started to appear from the proximal internal carotid artery. Regenerated aminergic nerve fibres were generally unbeaded and intensity of fluorescence was weak. The circular nerves appeared earlier than the longitudinal ones. The number of regenerating nerves reached the maximum, between months 9 and 12, at about half the normal level. AChE activity of the cerebral arteries showed no significant changes at any stage.

Acetylcholinesterase↗
PubMed1983

Intracranial adenoid cystic carcinoma. A case report.

A rare case of operatively verified intracranial adenoid cystic carcinoma is reported. In this case, haematogeneous metastasis to the brain parenchyma was suspected. The computer tomography showed a large hypodense mass in the left frontal lobe. The lateral wall of the tumour was irregularly enhanced with contrast media.

Brain Neoplasms↗
PubMed1983

A case of blue rubber bleb nevus syndrome with coronary artery fistula to left ventricle.

A 69-year-old female was admitted to the hospital because of tarry stool and dyspnea on exertion. Multiple hemangiomas were noticed on the tongue, right arm and abdomen. Gastrointestinal examination revealed multiple polypoid lesions in the stomach and small intestine. In addition, coronary artery-left ventricular fistula formation and pulmonary arteriovenous malformation were shown by angiography. Compared with other reported cases of blue rubber bleb nevus syndrome, the present case was unique because of the coronary artery-left ventricular fistula, heart failure and the familiar incidence of heart failure.

Aged↗
PubMed1983

A procedure for the isolation of primary cultures of melanocytes from newborn and adult human skin.

A method to obtain high-density primary cultures of adult and neonatal human melanocytes is described. Keratinocytes and melanocytes are coisolated from split-thickness sections fo neonatal foreskin obtained after circumcision, or from adult facial skin removed for cosmetic correction. Cells are released by trypsin and are plated in McCoy's 5A medium containing cholera enterotoxin (1 X 10(-9) M) and isobutylmethylxanthine (3.3 X 10(-5) M) to enhance melanocyte and keratinocyte multiplication. Keratinocytes and melanocytes are allowed to grow for 1 week. To remove associated keratinocytes, cells are exposed to 5-fluorouracil (5-FU) (1.92 X 10(-5), 3.84 X 10(-5), 1.92 X 10(-4) M). In the presence of low concentrations of 5-FU, keratinocytes are selectively destroyed within 3 weeks, while melanocytes continue to multiply and to form pigment. The differential effect of 5-FU on the multiplication of keratinocytes and melanocytes provides a simple method for obtaining cultures of melanocytes free from other nondendritic cell types present in the epidermis.

Adult↗
PubMed1983

Visualization of skin appendages by scanning electron microscopy.

A method for SEM visualization of skin appendages and elastic fibres around them is described. Skin sections were autoclaved for 4 h and followed by fixation, dehydration and xylol-air drying. Using this method, the three-dimensional architectural details of each type of skin appendage and related elastic fibre network were demonstrated.

Adult↗
PubMed1983

Analysis of antigenic determinants in cholera enterotoxin and heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Antigenic determinants of cholera enterotoxin (CT) and heat-labile enterotoxin from a human strain (LTh) and a porcine strain (LTp) were analyzed by Ouchterlony double-gel diffusion test against anti-CT, anti-LTh, and anti-LTp, which were treated by immunoaffinity column chromatography. The results showed the existence of the following antigenic determinants: (i) antigenic determinants unique to CT, LTh, and LTp, respectively; (ii) an antigenic determinant common to CT, LTh, and LTp; (iii) an antigenic determinant common to CT and LTh, but not LTp; and (iv) an antigenic determinant common to LTh and LTp, but not CT. On the basis of these results, an antigenic scheme for CT, LTh, and LTp is proposed.

Animals↗