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Biomedical subjects

T Tsuji

Publications and source records attributed to T Tsuji.

At least 919 records · Page 51Linked to original sources
PubMed1988

Quinone derivatives lower blood and liver acetaldehyde but not ethanol concentrations following ethanol loading to rats.

A rise in blood and liver acetaldehyde concentrations following an intragastric administration of ethanol to rats was significantly inhibited when the quinone derivatives 2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone (ubidecarenone, coenzyme Q10), 4,5-dihydro-4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylic acid (pyrroloquinoline quinone, PQQ) and 6-(10-hydroxydecyl)-2,3-dimethoxy-5-methyl-1,4-benzoquinone (idebenone) were injected intraperitoneally, prior to ethanol load, at a dose of 10, 11.5 and 30 mg/kg of body weight, respectively. When acetaldehyde was incubated in vitro with 1,4-benzoquinone (3.7-13.0 mM) or PQQ (1.4-4.9 mM) at 0 and 40 degrees C, the acetaldehyde concentrations slowly decreased with incubation time at 40 degrees C. The results suggest that low acetaldehyde concentrations following ethanol load are due to an accelerated oxidation of acetaldehyde by PQQ in the liver and the circulating blood.

Acetaldehyde↗
PubMed1988

Glycosaminoglycans partially substitute for proteoglycans in spheroid formation of adult rat hepatocytes in primary culture.

Adult rat hepatocytes seeded in a noncoated plastic dish containing serum-free medium formed a monolayer within 24 h of culture. Those seeded in a dish coated with a proteoglycan fraction isolated from rat liver reticulin fibers attached to the dish but did not spread within 4 h, and then gradually assembled to form floating spherical aggregates (spheroids) with a diameter of 120 +/- 40 micron, within 72 h. The proteoglycan fraction appeared to contain dermatan sulfate, heparan sulfate and an unidentified glycosaminoglycan in its glycan moieties by glycosaminoglycan analysis after pronase digestion and high molecular weight proteoglycan molecules (mw: over 300,000 and about 200,000) by SDS-PAGE analysis. Cells seeded in dishes coated with these defined glycosaminoglycans and heparin assembled to form hemispheroids and multilayer islands, but not floating spheroids, within 72 h of culture. Dermatan sulfate had a stronger ability to induce hemispheroids than heparan sulfate or heparin. As the hemispheroid and multilayer islands were the intermediate form between monolayer and floating spheroids, the glycosaminoglycan moieties of the proteoglycan fraction were thought to participate in the formation of spheroid.

Animals↗
PubMed1988

The QRS complex of the standard 12-lead electrocardiogram in septal myocardial infarction.

Interventricular septal involvement in myocardial infarction is suggested by the findings of a QS deflection in lead V1 and/or absence of the Q wave in leads I and V6, using the standard 12-lead electrocardiogram (ECG). However, these findings were not sufficiently established for the criteria of septal infarction, because several factors including anatomic position of the heart, changes of intraventricular conduction, and condition of the lung would affect the QRS complex in the leads. In this study, we analyzed the ECGs of anterior myocardial infarction with (group A) and without (group B) involvement of the first septal coronary artery. The R wave in V1 was absent in 64.7% of group A and 60.0% of group B. The Q wave was absent in 35.3% in lead I and 64.7% in lead V6 of group A, while in group B the Q wave in leads I and V6 were not observed in 60.0% and 40.0%, respectively. The prevalence rates of the R wave and the Q wave in these leads were not significantly different between the two groups. Left ventriculography revealed that the prevalence rate of the R wave in V1, and the Q wave in I and V6 was not significantly different, regardless of the presence or absence of impaired septal motion. Experimental study in anesthetized dogs confirmed the difficulty in clarifying acute septal ischemia by changes in the QRS complex. These results indicate that septal myocardial infarction is hardly detectable with changes in the QRS complex of the standard 12-lead electrocardiogram.

Coronary Vessels↗
PubMed1988

Phalloidin-induced alterations of bile canaliculi.

Phalloidin, a toxin from the plant Amanita phalloides, irreversibly polymerizes actin filaments and causes cholestasis. Three-dimensional structural changes induced by phalloidin in the bile canaliculi and the intra-acinar localization of these changes were studied in the rat liver by scanning and transmission electron microscopy. After 3 days of treatment, canalicular changes appeared mainly in zones 2 and 3 of Rappaport's acinus, but after 7 days of treatment changes occurred in bile canaliculi of the whole acinus. The changes in the bile canaliculi included tortuosity, saccular dilatation, loss of microvilli, bleb formation and elongation of canalicular side branches. Some side branches extended near to Disse's space, leaving only a thin cytoplasmic rim between the canalicular lumen and Disse's space. Kupffer cells were occasionally situated near such extended bile canaliculi and protruded their processes into the hepatic cord. These results suggest that bile canaliculi in zone 3 are more susceptible to phalloidin toxicity than those in zone 1 and that biliary constituents may leak from such altered bile canaliculi.

Animals↗
PubMed1988

Effect of heart rate and myocardial contractile force on coronary resistance.

The effect of the heart rate and myocardial contractile force on the extravascular resistance to blood flow of the left anterior descending coronary artery (LAD) was evaluated in 15 mongrel dogs anesthetized with sodium pentobarbital. The LAD was maximally dilated by intracoronary infusion of adenosine, which precluded the influence of vasomotor tone. Increases in the heart rate and myocardial contractile force decreased coronary blood flow in the absence of a change in coronary perfusion pressure. The changes in mean coronary resistance showed a significant linear relationship to changes in developed tension. The changes in coronary resistance caused by varying the heart rate and contractile force were so small that a normal coronary vascular tree could easily compensate for the increase in resistance. However, it is supposed that with critical stenosis of the vascular tree even a small increase in resistance might cause deleterious effects on coronary blood flow.

Adenosine↗
PubMed1988

Three-dimensional arrangement of ductular structures formed by oval cells during hepatocarcinogenesis.

The three-dimensional arrangement of ductular structures formed by oval cells in rats fed 2-acetylaminofluorene (2-AAF) was studied by scanning electron microscopy (SEM) of biliary tract casts and light microscopy of sections of liver injected with india ink via the biliary tract. Both resin and india ink were well injected up to bile ductules, and the findings of each method correlated with each other. By the second week after 2-AAF administration, a few oval cells appeared in the periportal areas forming ductular structures which connected with the portal bile ducts. At the 4th week, increased ductular structures occupied two thirds of the lobule and formed networks communicating with each other, and with the portal bile ducts. At the 8th week, such ductular structures were compressed around hyperplastic nodules and appeared like a basket in biliary casts examined by SEM. Although a histochemical study of gamma-glutamyl transpeptidase revealed activity both on the luminal side of the ductular structures and hepatocytes in hyperplastic nodules, no transition was observed between these two cell populations. These results suggest that oval cells have characteristics more similar to those of biliary epithelia than of hepatocytes, and have no relation to the development of hyperplastic nodules.

Animals↗
PubMed1988

Type IV collagen-degrading enzyme activity in human serum.

Type IV collagen-degrading enzyme activity was detected in human serum. Serum was preincubated with 4-aminophenylmercuric acetate and trypsin to activate the enzyme prior to assay. Type IV collagen, purified from human placentas and radiolabeled with [1-14C] acetic anhydride, was used as the substrate. The enzyme activity was measured at pH 7.5 and inhibited by treatment with ethylenediaminetetraacetic acid or heat. The assay of type IV collagen-degrading enzyme in human serum might be useful for estimating the degradation of type IV collagen.

Blood Proteins↗
PubMed1988

Serum type IV collagen-degrading enzyme in hepatocellular carcinoma with metastasis.

The activity of serum type IV collagen-degrading enzyme was measured in 18 patients with hepatocellular carcinoma (HCC). The enzyme activity was significantly higher, in HCC patients with a tumor thrombus in the portal vein than in healthy controls, liver cirrhosis patients and HCC patients without a tumor thrombus. Moreover, the activity in HCC patients with lung metastasis tended to be higher than that in HCC patients without lung metastasis. The activity of serum type IV collagen-degrading enzyme did not correlate with tumor size, serum alpha-fetoprotein level, or macroscopic classification of tumor growth. These results suggest that the activity of serum type IV collagen-degrading enzyme represents the metastatic potential or the ongoing metastatic activity of HCC. The enzyme is a useful serum marker of metastasis from HCC.

Aged↗
PubMed1988

Effect of branched-chain amino acid on 15N incorporation into liver and skeletal muscle proteins following [15N]-ammonium chloride administration to carbon tetrachloride-intoxicated rats.

The effect of branched-chain amino acid (BCAA) on protein synthesis and nitrogen metabolism in liver and skeletal muscle was evaluated by an intraperitoneal injection of [15N]ammonium chloride (15NH4Cl) to carbon tetrachloride (CCl4)-intoxicated rats. The 15NH4Cl was bolusly injected at a dose of 6 mg/100 g body weight one hour after an amino acid solution containing leucine and valine (150 mM each, abbreviated as BCAA), phenylalanine and alanine (150 mM each, PA), 120 mM leucine and 30 mM valine (Leu-rich) or 120 mM valine and 30 mM leucine (Val-rich) was administered intragastrially at a dose of 2.5 ml each/100 g body weight. The 15N-enrichment in the protein fraction of the liver was higher in CCl4-BCAA group than CCl4-PA group. The Leu-rich solution was found more effective in enhanced incorporation of 15N into liver and skeletal muscle proteins. The disappearance rate of [15N]urea from the plasma, which was influenced by the synthesis from 15NH3 and the excretion into urine, was much faster in the CCl4-BCAA group than CCl4-PA group. In Leu-rich group, both 15N incorporation into non-protein fraction of skeletal muscle and disappearance rate of plasma urea-15N were greater than those in Val-rich group. The results suggest that BCAA, particularly leucine, has beneficial effects on protein synthesis and ammonium detoxification in liver-injured rats.

Amino Acids, Branched-Chain↗
PubMed1988

cis-Halovinylthioacetamido side chain, a new effective structural element for 7 beta-substitution in cephem and oxacephem antibiotics. II. 7 beta-cis-Fluorovinylthioacetamino-7 alpha-methoxy-1-oxacephems.

The synthesis and in vitro activity of 1-oxacephem derivatives having a substituted or a non-substituted cis-fluorovinylthioacetamido side chain at C-7 are described. Of these new 1-oxacephem antibiotics, 2355-S (42a) shows good antibacterial activity against Gram-positive and Gram-negative bacteria, and very favorable pharmacokinetic properties.

Microbial Sensitivity Tests↗
PubMed1988

[An expression of the monoclonal antibody KM-93 reactive antigen in salivary gland tumors].

The expression of the antigen recognized by a monoclonal antibody KM-93 has been examined in salivary gland tumors by means of an immunohistochemical technique. In immunoperoxidase staining, KM-93 reacted strongly with mucoepidermoid carcinomas and papillary adenocarcinomas, but not with adenoid cystic carcinomas. Thus, this antibody may not meet the requirements as a tumor marker. The inner duct-lining cells of a pleomorphic adenoma expressed the antigen whereas its outer myoepithelial cells did not. In normal glands, neither mucous glands nor myoepithelial cells reacted with the antibody but serous glands and ducts stained positive with the antibody. KM-93, it is thought, would be found useful for differential diagnosis and histogenetic studies of salivary gland tumors.

Adenocarcinoma↗
PubMed1988

[The expression of monoclonal antibody KM-93 reactive antigen in human lung carcinoma--an immunohistochemical evaluation].

Using an histochemical technique, the expression of a tumor-associated antigen, recognized by the monoclonal antibody KM-93, has been investigated in 39 cases of human lung cancer. The overall positive rate was found to be 87.2%, this breaking down to 90.9%, 80.0%, and in adenocarcinomas, squamous cell carcinomas, and large cell carcinomas, respectively. In adenocarcinomas, most of the tumor cells stained strongly, while in squamous cell carcinomas, differentiated cells tend to react with the antibody. Even, in tumors judged to be negative, a small fraction of stained cells also was noted. These results suggest the clinical usefulness of this antibody.

Adenocarcinoma↗
PubMed1988

Enhancement of coronary conductance by alpha-human atrial natriuretic polypeptide without effects on myocardial contractility.

The effect of synthetic human atrial 28-amino acid peptide (alpha-human atrial natriuretic polypeptide, alpha-hANP) on coronary circulation and cardiac functions was examined in open-chest dogs. Intravenous injection of alpha-hANP increased coronary and systemic conductance, and coronary and aortic blood flow with a significant fall in blood pressure. Continuous infusion of alpha-hANP into the left anterior descending coronary artery (LAD) increased LAD blood flow in a dose-dependent manner. The linear regression analysis revealed the relationship of logit (changes in mean coronary conductance (delta MCC] = 1.45 x log (coronary plasma concentration of alpha-hANP) + 7.51 (r = 0.87, n = 29). REC50 of alpha-hANP was 5.1 microM, where REC50 was the concentration to increase MCC to a half maximum MCC during reactive hyperemia after a 30-s coronary occlusion. alpha-hANP increased coronary conductance with no changes of myocardial oxygen consumption (MVO2) when blood pressure remained constant. Indices of myocardial contractility measured with a strain gauge arch, myocardial force (F), max dF/dt and LV max dp/dt, were not altered by either bolus intravenous injection or continuous intracoronary infusion of alpha-hANP. These results indicated a direct increase by alpha-hANP of coronary and systemic vascular conductance.

Animals↗
PubMed1988

[A case of early gastric cancer accompanied by Recklinghausen's disease].

In September, 1980, a 57-year-old male, was admitted to hospital for precise examinations to determine if he had Recklinghausen's disease, pneumoconiosis, and a duodenal ulcer. Endoscopy revealed a redness and a shallow depression of the posterior wall of the antrum, and a biopsy of a specimen led to the diagnosis of early gastric cancer, Stage II c. After the examination, the patient did not return to our department again. In May, 1987, however, the man was readmitted to our hospital, suffering from pains in the right back. X-ray and endoscopic findings revealed a gastric cancer with a center depression and a peripheral elevation (II a + II c). Histological findings showed the gastric cancer to be a moderately differentiated tubular adenocarcinoma, with a submucosal invasion that was also seen. This case, uncovered 80 months earlier, represents a rare instance in which an early gastric cancer, accompanied by Recklinghausen's disease, expanded slowly.

Adenocarcinoma↗