Biomedical subjects

T Tsuda

Publications and source records attributed to T Tsuda.

789 records · Page 44Linked to original sources

PubMedDate not supplied

Alpha-naphthyl acetate esterase-1 (ANAE-1) secreted by epithelioid cells from induced rabbit lung granuloma showed MIF activity.

The function of alpha-naphthyl acetate esterase-1, whose isoelectric point values range from 5.15 to 5.45, was examined. A higher value of alpha-naphthyl acetate esterase-1 was detected in the extracts of epithelioid cells isolated from rabbit lung granuloma at 4 weeks after injection of Freund's complete adjuvant, compared to those values of alveolar macrophages isolated from the same lungs described above and of the normal lungs. Additionally, this enzyme activity was observed to be prominent in the culture supernatants of epithelioid cells. alpha-Naphthyl acetate esterase-1 was purified from lung granuloma as a single 62-kDa band by SDS-PAGE analysis. The purified enzyme showed a macrophage migration inhibition activity at concentrations over 20 nM, and its activity was dose-dependent. Moreover, when various amounts of the purified enzyme were added to lymphocyte-derived macrophage migration inhibitory factor, macrophage migration inhibition was significantly enhanced with a dose-dependent manner. The results suggest that alpha-naphthyl acetate esterase-1 secreted by granuloma macrophages, particularly by epithelioid cells, contributes to granuloma formation.

Animals↗

PubMedDate not supplied

Distribution of lysozyme and mucin (MUC2 and MUC3) mRNA in human bronchus.

Immunocytochemical studies have shown that gel-forming glycoproteins (mucins) and the bacteriolytic protein lysozyme are selectively expressed in airway mucous and serous cells, respectively. The mechanisms mediating this selectivity are unknown. In this study, we localized mucin and lysozyme mRNA by in situ hybridization to investigate the possibility that phenotype-specific expression of these proteins is controlled at the level of mRNA. Radiolabelled sense and antisense probes were constructed from the human tracheal mucin cDNA, HAM1 (MUC2 gene), the human small intestinal mucin cDNA, SIB139 (MUC3 gene), and the bovine tracheal lysozyme cDNA, Lys 7a. Frozen sections of human bronchus were hybridized with these probes and washed under routine conditions. Autoradiography showed that although lysozyme mRNA was strictly limited to cells expressing lysozyme, mucin mRNA was present both in mucin-expressing and mucin-non-expressing epithelial cells. This suggests that the restriction of lysozyme to serous cells is controlled at the level of mRNA (synthesis and/or degradation), whereas the restriction of mucin to mucous cells is controlled at the level of translation.

Blotting, Northern↗

PubMedDate not supplied

Identification of IL-1 inhibitory activity as IL-1 receptor antagonist (IL-1ra) in vitro and kinetics of IL-1ra and IL-1 beta in experimental rabbit lung granuloma.

This study investigated whether interleukin-1 (IL-1) inhibitory activity in LPS-stimulated culture supernatants of alveolar macrophages and epithelioid cells obtained from rabbit lung granulomas induced by complete Freund's adjuvant (CFA) was identical to IL-1 receptor antagonist (IL-1ra) and examined the changes of IL-1ra and IL-1 beta levels in lung tissue during the natural course of granulomatous inflammation. In the thymocyte proliferation assay, prostaglandin E2 (PGE2)-free culture supernatants from each cell population revealed a bell-shaped IL-1 titration curve with IL-1 activity suppressed at dilutions of 1:1 to 1:2, and gel chromatography of serum-free culture supernatants showed an IL-1 inhibitory peak at 21-25 kD. Suppression of IL-1 activity in the supernatants at lower dilutions and the gel-purified IL-1 inhibitory activity both almost disappeared after IL-1ra depletion with an anti-rabbit IL-1ra immunoaffinity column, indicating that IL-1ra was responsible for this in vitro IL-1 inhibitory activity. Pulmonary tissue levels of IL-1 beta pecked at 24 h (52.0 +/- 9.5 pg/mg) after CFA injection, whereas IL-1ra levels peaked at 4 weeks (23.1 +/- 4.0 ng/mg) when granuloma development was maximal, and the molar excess of IL-1ra to IL-1 beta peaked from 4 weeks onward at over 800-fold. These observations suggest that IL-1ra may be effective for IL-1 regulation, especially in the later stage of granulomatous responses. Immunohistochemical studies demonstrated that the cellular source of IL-1ra within the pulmonary granulomas was mainly epithelioid cells.

Animals↗

PubMedDate not supplied

Reduction in the incidence of Helicobacter pylori-associated carcinoma in Japanese young adults.

Helicobacter pylori (Hp) is a major risk factor for gastric carcinogenesis. In Japan, the incidence of Hp infection in young adults has declined markedly. The purpose of this study was to clarify this trend in the incidence of Hp-associated gastric carcinoma (GCa) in young (under 30 years of age) Japanese patients. We enrolled 53 such patients who underwent surgical resection of GCa in one of 18 hospitals in the Hiroshima prefecture between 1976 and 1999. The patients were classified into groups based on three 8-year periods (1976-83, 1984-91, and 1992-1999) in which their cases occurred. We compared the numbers of patients and estimated the histology of carcinomas, grades of gastritis and Hp infection histologically. Of the 53 patients, 49 (92%) showed Hp infection. The frequency of GCa in young adults has decreased gradually (21, 18, and 14 patients in 1976-83, 1984-91 and 1992-99, respectively). The numbers of Hp-positive carcinomas decreased radically (21, 17 and 11 patients, respectively). This trend was associated with improvement in the degree of gastritis in non-neoplastic mucosa. Of the four Hp-negative patients, three had signet ring cell carcinoma. Moreover, the numbers of patients with non-signet ring cell carcinoma also decreased (18, 12 and 7 patients in each period, respectively). These results suggest that Hp-associated carcinoma has declined gradually in young Japanese.

Adolescent↗

PubMedDate not supplied

[A case of McLeod syndrome].

A 52-year-old man was admitted to our hospital in 1997 because of high serum creatine kinase (CK) level. Neurologic findings revealed chorea on the trunk and extremities, mild weakness of proximal muscles, and absence of deep tendon reflexes in four extremities. Serum CK was elevated to 3,494 U/l (normal, below 235). A peripheral blood smear showed acanthocytes in approximately 1% of the red blood cells (RBCs). Very weak expression of the Kell antigens (K2, K4, K5, and K7) on his RBCs led us to make a final diagnosis of McLeod syndrome. Muscle biopsy from the left biceps showed increased variability in fiber diameter, a few regenerating fibers, scattered fibers with internal nuclei, and mild fiber type grouping. Immunohistochemical analyses of dystrophin. merosin, and adhalin were normal. Although McLeod syndrome is a rare X-linked recessive disorder, it is clinically important for differential diagnosis of chorea acanthocytosis and hyperCKemia.

Acanthocytes↗

PubMedDate not supplied

A case of intra-articular fracture of the knee joint with three layers within lipohemarthrosis by ultrasonography and computed tomography.

We report a case of intra-articular fracture of the knee joint showing three layers within lipohemarthrosis. Sagittal ultrasonography showed three layers (double fluid-fluid level): a superior hyperechoic layer of fat, an intermediate anechoic layer of serum, and an inferior hypoechoic layer of red blood cells. Horizontal computed tomography imaging demonstrated the same three layers. This is the first case of lipohemarthrosis in which three layers of joint effusion were confirmed by both ultrasonography and computed tomography.

Aged↗

PubMedDate not supplied

[Enuresis as the initial manifestation of neuro-Behçet's disease. A case report].

A 20-year-old man noticed enuresis since the age of 18 years. He was also pointed out gait unsteadiness by his friends. Neurologic examination revealed spastic tetraparesis with mild cerebellar ataxia. The cerebrospinal fluid had mild pleocytosis containing neutrophils and brain magnetic resonance imaging showed mild pontocerebellar atrophy. Urodynamic study revealed uninhibited bladder and detrusor-sphincter dyssynergia. The diagnosis of neuro-Behçet's disease was made by the past history of recurrent genital and aphthous ulcers and positive HLA-B51. The frequency of enuresis was reduced by the oral administration of corticosteroid. We speculated the patient's enuresis was caused by the central nervous disinhibition at night together with uninhibited bladder.

Adult↗

PubMedDate not supplied

Determination of inorganic tin in biological samples by hydride generation-atomic absorption spectrometry after silica gel cleanup.

A method is described for the determination of inorganic tin in biological samples by hydride generation-atomic absorption spectrometry (HG-AAS). A sample is extracted with ethyl acetate after addition of HCl and NaCl. The concentrated extract is passed through a silica gel column. The column is washed with ethanol, water, and 0.2N HCl successively, and then inorganic tin is eluted with 2N HCl and measured by HG-AAS. Recoveries from fish muscle spiked with 0.1 micrograms/g Sn4+ are 78.9 +/- 4.2% (average +/- standard deviation, n = 5). The detection limit is 0.01 micrograms/g as Sn.

Animals↗

PubMedDate not supplied

Simultaneous gas chromatographic determination of dibutyltin and tributyltin compounds in biological and sediment samples.

A method is described for the simultaneous determination of nanogram amounts of dibutyltin and tributyltin compounds in biological and sediment samples. These compounds are converted to the corresponding chlorides with HCl, extracted with ethyl acetate-hexane (3 + 2) for biological samples and with hexane for sediment samples, and hydrogenated with sodium borohydride. The corresponding hydrides, Bu2SnH2 and Bu3SnH, are detected by electron-capture gas chromatography after cleanup by silica gel column chromatography. Detection limits are 1.0-2.0 and 0.5-1.0 ng/g, respectively, for biological and sediment samples.

Animals↗

PubMedDate not supplied

Simultaneous gas chromatographic determination of carboxylic acids in soft drinks and jams.

A method was developed for simultaneous gas chromatographic determination of sorbic acid, dehydroacetic acid, and benzoic acid used as preservatives, and succinic acid, fumaric acid, malic acid, and tartaric acid used as acidulants in soft drinks and jams. A sample was dissolved in NH4OH-NH4Cl pH 9 buffer solution, and an aliquot of the solution was passed through a QAE-Sephadex A 25 column. The column was washed with water, and the carboxylic acids were eluted with 0.1N HCl. Sorbic acid, dehydroacetic acid, and benzoic acid were extracted with ethyl ether-petroleum ether (1 + 1), and determined on a 5% DEGS + 1% H3PO4 column. Succinic acid, fumaric acid, malic acid, and tartaric acid in the lower layer were derivatized with N,O-bis(trimethylsilyl)acetamide and trimethylchlorosilane, and determined on a 3% SE-30 column. Recoveries from soft drink and jam samples fortified with 0.1% each of 7 carboxylic acids ranged from 92.4 to 102.6% for preservatives, and from 88.1 to 103.2% for acidulants.

Beverages↗

PubMedDate not supplied

Separation of nucleotides by high-voltage capillary electrophoresis.

A new separation method using high-voltage capillary electrophoresis was applied to the analysis of several nucleotides. This system consisted of a micro-bore glass capillary column of 80-micron i.d., a high-voltage power supply, and a uv detector set at 254 nm. Seven nucleotides (cyclic AMP, AMP, ADP, ATP, GMP, GDP, and GTP) were separated completely from each other in 0.02 M phosphate buffer (pH 7) containing 0.5% ethylene glycol by applying about 150 V/cm. The theoretical plate number for AMP was nearly 45,000. A good separation of nucleotides in biological samples of rat blood, liver, and kidney was attained under the same conditions as above. Concentrations of nucleotides in these biological samples were measured by using pyridoxamine as an internal standard.

Animals↗

PubMedDate not supplied

Daily intakes of tributyltin and triphenyltin compounds from meals.

Daily intakes of tributyltin (TBT) and triphenyltin (TPT) compounds from meals in Shiga Prefecture, Japan, were investigated by 2 methods. Daily intakes of TBT and TPT by the duplicate portion method were, respectively, 4.7 and 0.7 microgram in 1991 and 2.2 and 0.7 microgram in 1992. Those by the market basket method were, respectively, 6.9 and 5.4 micrograms in 1991 and 6.7 and 1.3 micrograms in 1992. Daily intakes of TBT and TPT by the market basket method were higher than those by the duplicate portion method. These values were considerably lower than acceptable daily intakes of 80 micrograms/50 kg body weight specified by the Welfare Ministry of Japan for bis(tri-n-butyltin)oxide and of 25 micrograms/50 kg body weight specified by the Food and Agriculture Organization/World Health Organization for TPT.

Adult↗

PubMedDate not supplied

Market basket and duplicate portion estimation of dietary intakes of cadmium, mercury, arsenic, copper, manganese, and zinc by Japanese adults.

Daily intakes of selected metals from meals in Shiga Prefecture (Japan) were investigated by the duplicate portion method and market basket method. In 1991 and 1992, daily intakes of metals by women, determined by the duplicate portion method, were, respectively, 37 and 27 micrograms for Cd, 4.3 and 3.5 micrograms for Hg, 260 and 210 micrograms for As, 1200 and 1200 micrograms for Cu, 4700 and 3600 micrograms for Mn, and 8000 and 7200 micrograms for Zn. Those determined by the market basket method were, respectively, 32 and 35 micrograms for Cd, 4.3 and 9.9 micrograms for Hg, 160 and 280 micrograms for As, 1100 and 980 micrograms for Cu, 3600 and 4700 micrograms for Mn, and 8700 and 8500 micrograms for Zn. No wide differences between the 2 methods were found, except for Hg data in 1992. Daily intakes of Cd and Hg in Shiga Prefecture were lower than the provisional tolerable daily intakes of 57-72 micrograms for Cd and 43 micrograms for Hg, proposed by the Food and Agricultural Organization and the World Health Organization.

Adult↗

PubMedDate not supplied

Levels of Cd, Hg, As, Cu, Mn, and Zn in Japanese restaurant and box lunches.

Intakes of selected elements from restaurant and box lunches in Shiga Prefecture (Japan) were investigated in 1993 and 1994, respectively. Average intakes of these elements from restaurant and box lunches were, respectively, 14 and 12 micrograms for Cd, 4.6 and 4.5 micrograms for Hg, 85 and 91 micrograms for As, 461 and 561 micrograms for Cu, 1268 and 1235 micrograms for Mn, and 3612 and 2688 micrograms of Zn. There were no great differences between the intakes from the restaurant lunches in 1993 and the box lunches in 1994. The proportion of elements from the lunches constituted 25-38% of the daily intake for Cd, As, Mn, and Zn and 51-65% of the daily intake for Hg.

Arsenic↗

PubMedDate not supplied

Novel instrumentation for measurement of diffusion coefficient by capillary zone electrophoresis and its application to aqua lanthanide ions.

The novel, modified instrumentation of a capillary zone electrophoresis (CZE) system and theory are proposed for the measurement of diffusion coefficient. The solute zone in capillary tubing is forced back and forth repeatedly by the aid of negative and positive applied high voltage, respectively, and the peak widths are recorded for the estimation of diffusion coefficient. The diffusion coefficients of Ce(III) and Cu(II) in aqueous solution were determined, and the former hydration number of Ce(III) was estimated by comparison with both diffusion coefficients.

Cerium↗