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Biomedical subjects

T Tsuchiya

Publications and source records attributed to T Tsuchiya.

At least 775 records · Page 43Linked to original sources
PubMed1981

Renal excretion and distribution of ceftizoxime in rats.

A clearance method for ceftizoxime (CZX), a new cephalosporin antibiotic, was designed and utilized to assess the mechanism of renal excretion and evaluate kidney distribution under steady-state serum level. Renal clearance ratio of CZX to inulin, when corrected for protein binding, was 1.6 at serum level of 13 micrograms/ml and was decreased to around unity with a serum level of 700 micrograms/ml or higher or by infusing p-aminohippuric acid or probenecid. The clearance ratio appeared to be independent of urinary pH or changes in urinary flow. The kidney/serum concentration ratio for CZX decreased from 2.5 to 1.5 in proportion to the extent of reduction of the clearance ratio. p-Aminohippuric acid and probenecid further reduced the kidney/serum ratio to about 1.0 with concomitant inhibition of tubular transport of CZX. Cortex/serum and medulla/serum ratios were also reduced similarly to that of the whole kidney. It is concluded that the tubular transport of CZX is similar to that for p-aminohippuric acid transport system and that passive reabsorption from the tubular lumen may not necessarily be present.

Animals↗
PubMed1980

Ultrastructural localization of alkaline phosphatase activity in sheep parathyroid gland.

The histochemical localization of alkaline phosphatase in sheep parathyroid gland was investigated using light and electron microscopy. The reaction products of enzyme activity were observed by light microscopy in pericytes. By electron microscopy they were limited to the intercellular spaces between the gland cells, being exclusively confined to the external surface of plasma membrane.

Alkaline Phosphatase↗
PubMed1980

Two new C3H mouse ascites tumor cell lines capable of proliferation in vivo and in suspension culture: morphological, karyological, kinetic and immunological properties.

The establishment of mouse tumor cell lines capable of proliferating in vivo and in suspension culture was undertaken. The MM-46 tumor line, initiated from primary mammary carcinoma arising in a C3H/He mouse, was maintained for over 100 generations in the peritoneal cavities of syngenic mice. At the 50th generation of the tumor suspension, cultures were initiated. The established cell lines, designated TMT-1 and TMT-2, were characterized in vitro and in vivo. The morphological finding indicated that THT-1 and TMT-2 cells from mice closely resembled the MM-46 tumor cells. The oncogenic potential of the cultured cells was comparable to that of the original ascites tumor. The population doubling time of TMT-1 and TMT-2 cell lines was about 12 hr in mice, whereas the population doubling time of both cell lines lengthened to 20 hr in suspension culture. The increase in doubling time in culture was due to the prolongation of the G1 period. The cell lines, TMT-1 and TMT-2, whether fropm culture or mice, possessed colony forming ability in in soft agar medium. The colony forming ability of the cells decreased gradually through in vivo passages but it recovered upon recultivation of the cells from mouse to culture. Chromosome analysis and cytotoxicity test by anti-MM antiserum indicated that TMT-1 and TMT-2 cell lines closely resembled and had been derived from MM-46 tumor line. Therefore, it is possible to assay cell survival in vitro after in vivo experiments on these cells.

Animals↗
PubMed1980

Long-term effects of moderate X-ray doses on the averaged evoked potentials in the rabbit.

The long-term effects of moderate X-ray doses on the averaged evoked potentials (AEPs) recorded from the visual cortex to photic stimulation were studied in 6 adult male Japanese white rabbits with permanently implanted electrodes. One group of 3 animals received 100 R to the whole brain, and another group of 3 received 300 R to the lateral half of the brain. Two of the 3 animals irradiated with 300 RT were used for the study of the recovery cycle (RC) of the AEPs. The RC of the AEPs was obtained with 4 photic stimulus intervals ranging from 110 to 750 msec. The amplitude of the late two components (IV and V) of the AEPs of the 3 animals irradiated with 100 R changed little during the first several months. It showed a gradual decrease thereafter, with individual variations as to degree. The associated peak latencies were even less affected by the radiation. The amplitude of the late two components recorded from the irradiated and unirradiated cortices of the 3 animals exposed to 300 R gradually decreased in the course of time after irradiation. No differences were observed between the changes in the irradiated and unirradiated cortices. The peak latency of some of the 5 components in 2 of the 3 animals showed a slight change after exposure. The recovery ratio of the amplitude of the late two components at a stimulus separation of 220 msec recorded from the irradiated and unirradiated cortices in one of the 2 animals increased 1 month after exposure. It remained grossly unchanged thereafter. There were no differences between the changes in the irradiated and unirradiated cortices. All 6 animals died between 181 and 584 days after exposure.

Animals↗
PubMed1980

Melibiose transport of Escherichia coli.

Transport of [3H]melibiose, prepared from [3H]raffinose, was investigated in Escherichia coli. Na+ stimulated the transport of melibiose via the melibiose system, whereas Li+ inhibited it. Kinetic parameters of melibiose transport were determined. The Kt values were 0.57 mM in the absence of Na+ or Li+, 0.27 mM in the presence of 10 mM NaCl, and 0.29 mM in the presence of 10 mM LiCl. The Vmax values were 40 and 46 nmol/min per mg of protein in the absence and in the presence of NaCl and 18 nmol/min per mg of protein in the presence of LiCl. Melibiose transport via the melibiose system was temperature sensitive in a wild-type strain of Escherichia coli and was not inhibited by lactose. On the other hand, melibiose uptake via the lactose system was not temperature sensitive, was inhibited by lactose, and was not affected by Na+ and Li+. Methyl-beta-D-thiogalactoside, a substrate for both systems, inhibited the transport of melibiose via both systems.

Biological Transport, Active↗