While the relationship between intraocular pressure and plasma osmolarity has been investigated during hemodialysis, no research has been published concerning the relationship between intraocular pressure and plasma colloid osmotic pressure during hemodialysis. We describe a patient with neovascular glaucoma who demonstrated a marked decrease in intraocular pressure during hemodialysis. The patient's intraocular pressure, plasma osmolarity, plasma colloid osmotic pressure, and body weight were evaluated every 30 minutes during hemodialysis lasting 4.5 hours. Plasma osmolarity was almost constant during hemodialysis (beginning: 295 mOsm/L; ending: 305 mOsm/L), and plasma colloid osmotic pressure increased (beginning: 23.0 mm Hg; peak: 27.2 mm Hg). Weight loss was 5.7 kg. Intraocular pressure decreased markedly during hemodialysis (beginning: 53 mm Hg; ending: 14 mm Hg). This sharp decrease in intraocular pressure and the increase in plasma colloid osmotic pressure, indicate a relationship between plasma colloid osmotic pressure and the change in intraocular pressure during hemodialysis.

Blood↗

PubMedDate not supplied

Alkaloids from dendrobatid frogs: structures of two omega-hydroxy congeners of 3-butyl-5-propylindolizidine and occurrence of 2,5-disubstituted pyrrolidines and a 2,6-disubstituted piperidine.

Forty alkaloids were detected and characterized from skin extracts of high- and low-elevation populations of the poison frog Dendrobates histrionicus from northwestern Colombia. Combined gc/ms with NH3 or ND3 in a chemical ionization mode detected protonated parent ions and determined the number of exchangeable NH and OH hydrogens. Six previously unknown dendrobatid alkaloids were characterized. Two were 2,5-disubstituted pyrrolidines, which included pyrrolidine 197B, a trans-2-butyl-5-pentylpyrrolidine, while a third was a 2,6-dipentylpiperidine. Indolizidines 239AB and 239CD had the same relative configuration as the parent alkaloid 223AB [(5E,9E)3-butyl-5-propylindolizidine] and contained, respectively, a omega-hydroxy group in the propyl or butyl side chain. The profiles of alkaloids in the new northern populations of D. histrionicus are typical of the species in containing a set of about eight histrionicotoxins, in marked contrast to a related species, Dendrobates lehmanni, which does not contain histrionicotoxins.

Alkaloids↗

PubMedDate not supplied

Cdc6 expression as a marker of proliferative activity in brain tumors.

Antibodies against human proteins that regulate DNA replication such as Cdc6 and Mcm5 became available as a new marker of proliferation. We performed immunohistochemical analysis with MIB-1 and antibody against Cdc6 on 35 brain tumors, including tumors of neuroepithelial tissue, vestibular schwannomas, meningiomas, and pituitary adenomas. Median reactivity for MIB-1 was 8.8%, and that for Cdc6 was 55%. Reactivity in most brain tumors was significantly higher for Cdc6 than for MIB-1, but reactivity of Cdc6 was independent of tumor grade. Detection of Cdc6 expression might be useful for the estimation of proliferative activity in brain tumors.

Antigens, Nuclear↗