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T Terashima

Publications and source records attributed to T Terashima.

At least 199 records · Page 11Linked to original sources
PubMed1984

Observations on the brainstem-spinal descending systems of normal and reeler mutant mice by the retrograde HRP method.

Brainstem neurons which project to the lumbar spinal level were identified in both reeler mutant mice and normal controls (Balb/c mice) by the retrograde horseradish peroxidase (HRP) technique. In normal controls after HRP injection into the lumbar cord, retrogradely labelled neurons were observed in (1) the lateral vestibular nucleus, (2) the pontine and medullary reticular formations including the nucleus centralis caudalis pontis, nucleus gigantocellularis, nucleus paragigantocellularis, nucleus raphe magnus et pallidus, and nucleus centralis medullae oblongatae pars ventralis et dorsalis, and (3) the dorsal column nuclei, i.e., the nucleus gracilis and nucleus cuneatus medialis. In reeler mutant mice, labelled neurons were again seen in the nuclei referred to above, and their cellular type and distribution patterns within the corresponding nuclei were similar to those of the normal controls. These observations suggest that (1) the brainstem nuclei of reeler mutant mice which project to the lumbar spinal cord are cytoarchitecturally normal, (2) the reeler genetic locus (rl) does not affect the nonlaminated structures in the brainstem, at least those referred to above, and (3) the motor dysfunctions observed in the reeler, such as action tremor, dystonic posture, and reeling ataxic gait, are not attributable to the brainstem-spinal descending systems.

Animals↗
PubMed1984

Quantitative analysis of synaptic boutons on motoneurons of rhesus monkey spinal cord after chronic deafferentation due to cerebral lesions.

The motoneurons in the lateral nucleus of the anterior horn of the rhesus monkey cervical enlargement were reconstructed by light microscopy of semi-thin sections of 2 micro in thickness which were cut serially, alternately with ultra-thin sections for electron microscopy. Four single neurons were reconstructed with a computer graphic system, and simultaneously the synaptic boutons on the soma and each dendrite of single reconstructed neurons were analyzed quantitatively by electron microscopy. The synaptic boutons were classified into 4 types on the basis of the shape of the synaptic vesicles. The F-type boutons with flattened vesicles, S-type with spherical vesicles, G-type with cored vesicles and large L-type boutons with smaller and polymorphic vesicles, were almost identical to the boutons categorized by Bodian (1966b). The F-type boutons represented the major type in terms of the covering ratio and synaptic population. The S-type boutons tended to occur more frequently on dendrites. The motoneurons of the same region were also investigated in a similar manner after chronic deafferentation by the ablation of the upper extremity area of the left cerebral cortex. F-type of boutons were significantly increased, especially on the dendrites, compared with the synaptic distribution of the normal motoneurons.

Animals↗
PubMed1984

[A case report of the incomplete double aortic arch].

A case of the incomplete double aortic arch was observed on a male Japanese subject (69 years old) in students' dissection of Hokkaido University School of Medicine. The ascending aorta was directly followed by the right aortic arch, from which the left brachiocephalic trunk, right common carotid artery and right subclavian artery successively arose. As the cord-like structure (8 mm in length, 2 mm in diameter) of the left aortic arch was found between the left subclavian artery and descending aorta, the left brachiocephalic trunk seemed to be essentially equivalent to the initial portion of the left aortic arch. Thus, this anomaly of the aortic system was concluded as the incomplete double aortic arch. Through the vascular ring formed by the cord-like left aortic arch and right aortic arch, the trachea and esophagus passed. So-called aortic diverticulum was found at the site where this cord-like structure attached to the descending aorta. Histological examinations of the cord-like left aortic arch demonstrated that (a) it was completely closed and any structure suggesting to the tunica intima could not be also identified; (b) elastic and collagen fibers were scattered within the stroma of this structure, and somewhat concentrically arranged at its peripheral margin; and (c) several small blood vessels penetrated into this structure. The ligamentum arteriosum normally arose from the left pulmonary trunk, and connected with the aortic diverticulum just described above. The left and right recurrent laryngeal nerves respectively looped under the ligamentum arteriosum and right aortic arch. The thoracic duct did not open into the left venous angle, but into the right one.

Aged↗
PubMed1983

Distribution and morphology of corticospinal tract neurons in reeler mouse cortex by the retrograde HRP method.

Reeler, a recessive mutation in mice, causes cytoarchitectonic abnormalities to the cerebral and cerebellar cortices. In normal controls, corticospinal (CS) tract neurons retrogradely labelled after HRP injection into the lumbar cord were situated only in layer V (the inner pyramidal layer). In the reeler, by contrast, the labelled CS neurons were scattered diffusely throughout all levels of the corresponding cortical area. In addition to the malpositioning of the somata, the labelled CS neurons in the cortex of the reeler could be divided into two major classes according to their dendritic pattern: typical pyramidal neurons and atypical ones. The typical pyramidal neuron had an apical dendrite projecting from the superior pole of the soma and ascending toward the pia mater and several basal and basolateral dendrites projecting from the inferior pole of the soma. The atypical neurons consisted of six types: (1) inverted, (2) tumbled, (3) bipolar, (4) V-shaped, (5) hook-shaped, and (6) superficial polymorphic. The typical pyramidal neurons in the reeler tended to be situated relatively deep in the cortex and the atypical neurons tended to lie relatively superficially in the cortex. The axons of both the typical pyramidal neurons and the atypical ones in the reeler usually extended from the lower surface of the soma or one of the descending dendrites as in the normal control.

Animals↗
PubMed1983

Developmental changes of oligodendroglia in the posterior funiculus of "Shiverer" mutant mouse spinal cord, with special reference to myelin formation.

Oligodendroglia in the "Shiverer" spinal cord at 2, 4, 8 and 18 weeks of age were investigated by electron microscopy, with special reference to the process of myelin formation. The predominant changes observed were vacuolization of the cytoplasm during 2 and especially 4 weeks of age, indicating initial steps in the formation of myelin sheaths, although their total volumes was very small and their lamellae were incomplete. Polymorphic vacuoles and vesicles with various electrondense contents appeared to originate from the Golgi complex and the rough-surfaced endoplasmic reticulum (r-ER), since such vacuoles happened to be continuous with the Golgi complex, and the Golgi complex disappeared concomitantly with the appearance of great numbers of vacuoles. The r-ER was also fragmented together with the occurrence of vesicles. After 8 weeks of age, the number of myelinated nerve fibers reached the plateau of the increasing curve, while the number of myelin lamellae successively increased until the sheaths were completed. At these stages the oligodendroglia containing vacuoles gradually disappeared, although numerous oligodendroglia possessing a nucleus with peripheral, densely clumped chromatin were present. These dark oligodendroglia contained a Golgi complex, r-ER with small, single cisternae and microtubules of irregular thickness, different from those found in the control oligodendroglia. At 18 weeks of age polymorphic vacuoles had disappeared in most oligodendroglia.

Animals↗
PubMed1982

Method for determination of angiotensin-converting enzyme activity in blood and tissue by high-performance liquid chromatography.

A simplified method for an angiotensin-converting enzyme activity assay in biological samples was developed. Samples were incubated with hippurylhistidylleucine, an artificial substrate of angiotensin-converting enzyme. The reaction was terminated by the addition of metaphosphoric acid and liberated hippuric acid in the supernatant was quantitated directly by reversed-phase high-performance liquid chromatography. Tissues were homogenized in the presence of Nonidet-P40, a detergent, and the resulting supernatant was used for the assay of tissue angiotensin-converting enzyme activity by high-performance liquid chromatography. The present procedure made it possible to determine angiotensin-converting enzyme activity in whole blood and the total activity in tissues. A comparative study of angiotensin-converting enzyme activity in plasma, kidney and lung of five experimental animals showed a high degree of variation from species to species.

Animals↗
PubMed1982

Appearance of enterotoxigenic Escherichia coli in piglets with diarrhea in connection with feed changes.

Twelve weaned piglet (3-week-old) were divided into three groups according to time of feed change and observed for diarrhea during the time they were 3 to 8 weeks of age. A total of 553 strains of Escherichia coli were isolated from rectal fecal samples and examined for heat-labile (LT) and heat-stable (ST) enterotoxins, pilus antigens (K88, K99, and 987P), hemolysin (Hly), raffinose utilization (Raf) and drug resistance. Enterotoxins and/or hemolytic E. coli strains appeared in the rectal feces of 5- to 6-week-old piglets with diarrhea in connection with feed change and changing temperatures. Most of the isolates showed multiple drug resistance to sulfonamides (Sa), streptomycin (Sp), ampicillin, and/or mercury. Enterotoxigenic E. coli isolates represented four phenotypes: K88+.LT+.Hly+.Raf+.(SaSmCpTcKmSp) (12 strains), K99+.ST+.Raf+.(TcKm) (7 strains), ST+.Raf+.(TcKm) (7 strains), and ST.+(SaSmKm) (25 strains). The drug resistance determinants were transferable concurrently and some of them mobilized the determinants for K88, LT, Hly, and Raf to an E. coli C strain.

Animals↗
PubMed1982

Circulating immune complex in glomerulonephropathy associated with hepatitis B virus infection.

Urinary abnormalities combined with positive serum hepatitis B virus surface antigen (HBsAg) were found in 9 children. Most of these patients were found by mass urine screening of school children. Renal biopsy findings revealed 6 cases of membranous glomerulonephropathy (MGN) and 3 cases of minimal change disease. Immune deposits consisting of hepatitis B virus e antigen (HBeAg), IgG and C3 were detected in the glomerular capillary walls by immunofluorescent study in patients with MGN. Circulating immune complex was positive at a rate of 67% (6/9 cases) by the C1q-binding assay and at a rate of 56% (5/9 cases) by the protein-A precipitation test in patients with persistent urinary abnormalities and hepatic dysfunction.

Antigen-Antibody Complex↗