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Biomedical subjects

T Terao

Publications and source records attributed to T Terao.

At least 289 records · Page 16Linked to original sources
PubMed1993

[A new histologic diagnosis for amniotic fluid embolism by monoclonal antibody TKH-2 that recognizes mucin-type glycoprotein].

Sialyl Tn structure (NeuAc alpha 2-6 GalNAc 1-0-Ser/Thr) recognized by monoclonal antibody TKH-2 is a characteristic component in meconium- and/or amniotic fluid-derived mucin. The purpose of this study was to evaluate whether immunohistochemical staining employing antibody TKH-2 is a sensitive method for histological diagnosis of amniotic fluid embolism (AFE). The intravenous injection of 10% meconium solution (1 ml/kg) into rabbits was carried out. Formalin-fixed, paraffin-embedded lung, liver, and kidney tissue sections were obtained at various time interval after injections. Specimens were stained by the Streptavidin Biotin Complex method. The results of immunostaining were compared to those of hematoxylin and eosin (H & E) stain or a conventional Alcian blue stain. Remarkable positive stainings were easily seen within the pulmonary artery vasculature in experimental rabbits. Meconium-derived mucin can be easily missed on H & E sections. Compared to TKH-2 staining, Alcian blue staining may also be insufficient to demonstrate intravascular mucin in the lung sections. TKH-2 staining is the most sensitive method to use in detecting meconium-derived mucin in the lung sections of rabbits with an AFE experimental model.

Amniotic Fluid↗
PubMed1993

[Syphilitic orchitis: report of a case].

Syphilitic orchitis ir recently a rare disease in Japan. A 44-year-old man visited our hospital with the complaint of swelling of the right scrotum. He first noticed it about a half year ago, but the swelling mitigated without any therapy. The right testis was about the size of a hen's egg, elastic hard, and slightly rugged when he first visited our hospital. Treponema pallidum hemagglutination assay (TPHA) and rapid plasma reagin (RPR) were positive. We performed high orchiectomy because we could not deny the possibility of testicular tumor. Pathologically, granulomatous inflammation with lympho-plasmacytic infiltration and endoarteritis obliterans of the small arteries were seen in the specimen. It was diagnosed as granulomatous inflammation possibly caused by syphilitic orchitis. Syphilitic orchitis is usually quite difficult to distinguish from other types of granulomatous diseases of the testes, for example tuberculous orchitis. Various types of stains and careful examination of the specimen were thought to be necessary to distinguish these diseases.

Adult↗
PubMed1993

[Heterogeneity of the CA125 antigen that coexpresses sialyl Tn and CA19-9 antigens].

High molecular mass glycoproteins detected by the monoclonal antibody OC125 were found in secreted and solubilized materials from the ovarian cancer cell line, HOC-I. The CA125 antigen has been isolated from HOC-I cell extract and conditioned media by perchloric acid precipitation, gel filtration, and OC125 affinity purification. The higher molecular mass complexes (molecular masses were estimated to be > 1,000KDa) was predominantly found in extracts of cells grown in vitro or in ovarian cancer tissues, whereas the lower molecular mass antigen (molecular masses were estimated to be 110-400KDa) was the major component in conditioned media and sera from patients with ovarian cancer. Double determinant enzyme-linked immunosorbent assays were carried out with several antibodies (OC125, NS19-9, TKH-2, B72.3, CC49, MA54, MA61, PC47H) immobilized on a solid phase to bind different epitopes on antigens in clinical samples. Biotinylated OC125 was used as a probe to detect the CA125 antigen that had been bound via different epitopes. When TKH-2 was used to bind the antigen and OC125 used as a probe, the coexpression of CA125/sialyl Tn was observed in 12(22%) of 55 sera from patients with ovarian cancer. In some cases, sialyl Tn or CA19-9 antigens may be present on the CA125 macromolecular complexes.

Antibodies, Monoclonal↗
PubMed1993

[Idiopathic thrombocytopenic purpura (ITP) associated with renal injury: report of a case].

A 42-year-old woman with gross hematuria, left flank pain and vomit after being hit on her back admitted to our hospital. She has been under treatment for idiopathic thrombocytopenic purpura (ITP) with prednisolone for these last 14 years. Computerized tomographic (CT) scan revealed moderate laceration, subcapsular hematoma and perirenal hematoma of the left kidney. We tried treating her conservatively. The main treatment was absolute rest for two weeks, increase in the prednisolone dose and prevention from infection. On the 3rd day, the platelet count dropped to 4,000/mm3, but transfusion of platelets was not done to provide for emergency. The platelet count increased gradually and returned to normal on the 8th day. We decreased the prednisolone dose according to the platelet count. After 5 weeks, microscopic hematuria disappeared. Her clinical course was uneventful and she was discharged after 7 weeks.

Accidental Falls↗
PubMed1992

Cytosolic and intranuclear calcium signals in rat basophilic leukemia cells as revealed by a confocal fluorescence microscope.

A confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) was used to study spatial heterogeneity of the calcium signals in rat basophilic leukemia 2H3 cloned cell line (RBL-2H3). After stimulation with antigen (2,4-dinitrophenol-conjugated bovine serum albumin), fluo-3-fluorescence intensities increased in individual RBL-2H3 cells with different lag times. Time-dependent profiles of the fluo-3-fluorescence intensities resembled closely the patterns of the sequential fluorescence-ratio images of fura-2, which were used to measure the intracellular free-calcium concentration ([Ca2+]i) in individual RBL-2H3 cells using a conventional fluorescence microscope. The present results obtained using the confocal fluorescence microscope showed spatial heterogeneities of fluo-3-fluorescence intensities, suggesting the existence of spatial heterogeneity of [Ca2+]i in RBL-2H3 cells. That is, the results showed that calcium signals first occurred transiently at pseudopodia in RBL-2H3 cells, then the signals transferred to the central parts of the cells. In addition, from the fluorescence images of co-loaded Hoechst 33342 (bisbenzimide H 33342, a DNA-specific probe) which were produced by excitation with a He-Cd laser, it was found that the fluorescence images of the nucleus were quite similar to those of the calcium signals mentioned above. This suggested that the receptor-mediated calcium signals were transferred not only to the cytoplasm but also to the nucleus.

Animals↗
PubMed1992

Inhibition of in vitro ovarian cancer cell invasion by modulation of urokinase-type plasminogen activator and cathepsin B.

HOC-I ovarian cancer cells express the single-chain form of the urokinase-type plasminogen activator (uPA) and cathepsin B (cath B) on their cell surface. The significance of the expression of cell surface uPA/cath B activity to the invasive potential was examined by preincubating with uPA/cath B-modulating agents in in vitro invasion assay. The anti-uPA monoclonal antibody 394 effectively inhibited invasion in a dose-dependent manner. On the contrary, anti-cath B antibody did not affect the invasive potential of the cells. E-64, a specific inhibitor for cysteine proteases, blocked invasion as effectively as monoclonal antibody 394. The data reveal that the uPA and cysteine proteases contribute significantly to the invasive capacity of the cells. We suggest that the cysteine proteases facilitate the action of uPA, possibly by activating proenzyme uPA produced by cancer cells. Evidence for the role of a cathepsin-uPA activation cascade in HOC-I cell invasion is provided.

Cathepsin B↗
PubMed1992

Antitumor activity of a novel nucleoside, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosylcytosine (CNDAC) against murine and human tumors.

The antitumor effects of 2'-C-cyano-2'-deoxy-1-beta-D- arabinofuranosylcytosine (CN-DAC), a synthetic 1-beta-D-arabinofuranosyl-cytosine (ara-C) derivative, were examined and compared with that of ara-C in murine tumors and in various human tumors using three different chemosensitivity tests. CNDAC extended the life span of mice bearing P388 leukemia. CNDAC had a unique in vitro antitumor spectrum for human cancers different from that of ara-C. Compared with ara-C, CNDAC was more effective in 10 human tumors (2 lung, 4 stomach and 4 osteosarcoma), equal in 2 tumors (lung and fibrosarcoma) and less potent in 11 tumors (4 lung, 4 osteosarcoma, bladder, renal and epidermoid). Characteristically CNDAC showed excellent activities against tumors, refractory to ara-C, such as HT-1080 human fibrosarcoma implanted in chick embryos or athymic mice, although its cytotoxicity against HT-1080 was almost equal to that of ara-C. Thus, CNDAC is an interesting and promising agent that should be considered for further detailed preclinical evaluation.

Animals↗
PubMed1992

Lithium chloride stimulates catecholamine synthesis and secretion in cultured bovine adrenal medullary cells.

We examined the effects of lithium treatment on the synthesis and secretion of catecholamines in cultured bovine adrenal medullary cells. The treatment of cells with lithium (0.5-4 mmol/L) for 7 days caused an increase in basal and carbachol-stimulated synthesis of 14C-catecholamines from [14C]-tyrosine but not from [14C]-DOPA. Lithium treatment (4 mmol/L, 7 days) increased the activity of tyrosine hydroxylase in the cells. Lithium treatment (2-4 mmol/L, 7 days) also enhanced the secretion of catecholamines caused by carbachol, although the carbachol-induced influx of 45Ca2+ was reduced. Lithium (4 mmol/L, 7 days) potentiated the secretion of catecholamines evoked by the Ca2+ (1 mumol/L) from cells that were permeabilized by digitonin. The activity of protein kinase C in a soluble fraction was increased in lithium-treated cells (4 mmol/L, 7 days). These results demonstrate that lithium treatment increases the synthesis and secretion of catecholamines and the activity of protein kinase C in cultured adrenal medullary cells.

Adrenal Medulla↗