Biomedical subjects

T Tateshita

Publications and source records attributed to T Tateshita.

22 records · Page 2Linked to original sources

PubMed1996

Promotion of the osteogenetic activity of recombinant human bone morphogenetic protein by basic fibroblast growth factor.

In this study, we investigated the promotive effect of basic fibroblast growth factor (bFGF) on the osteogenetic activity of bone morphogenetic protein (BMP) used in conjunction with porous hydroxyapatite ceramic pellets. Results showed that there was extensive bone formation as early as 3 weeks after insertion in the group that received pellets treated with a relatively large amount of recombinant human (rh) BMP alone as well as in the group in which pellets were treated with a small amount of rhBMP combined with rhbFGF. Subsequently, osteogenesis within the pores of the pellets of the two groups slowly progressed over time; by 9 weeks after insertion, most of the pellet pores were filled with newly generated bone. In contrast, the group that received pellets treated with rhbFGF alone showed little osteogenesis, as was the case with the control group. The group that received the pellets treated with the small amount of rhBMP as well as the rhbFGF group exhibited significantly greater bone induction than the group that received the pellets treated with the small amount of rhBMP alone. These results indicated that a very small amount of rhbFGF has a strong promotive effect on the osteogenetic activity of rhBMP.

Analysis of Variance↗

PubMed1995

A new operative method for treating severe cryptotia.

Results following surgical treatment for cryptotia are as yet far from fully satisfactory in cases in which deformity of the upper auricular portion is severe and particularly when there is contraction of the helical skin and auricular cartilage. Therefore, improvements in operative procedures have been considered necessary. We have developed a new method for treating cryptotia using a rhomboid flap in the superior and anterior auricular regions to correct the contraction of skin in the helix in cases in which the deformity of the upper auricular portion is large and shortening in the anterior and posterior directions is significant and in which severe contraction of the helix is observed. In this report, we describe the operative procedures used and present the relatively favorable results obtained in three patients. Using these techniques, we have been able to elongate the contracted helix frequently observed in cases of cryptotia by preparing a flap in the anterior auricular region. This approach appears to be a useful and safe means of treating cryptotia and so-called constricted ear as well.

Child, Preschool↗

PubMedDate not supplied

Effect of a collagen matrix containing epidermal growth factor on wound contraction.

Excessive wound contraction is known to lead to pathological wound contracture. Using a rabbit model, we applied a bovine type I collagen matrix sponge as a dermal substitute and human epidermal growth factor to full-thickness excisional wounds. Wound contraction was assessed 14 and 28 days after wounding. It was found that both collagen matrix and epidermal growth factor significantly inhibited wound contraction (p < 0.001) in all wounds treated with collagen matrix alone or treated with 0.1 and 1 microg of epidermal growth factor 28 days after wounding. Interestingly, the combination of collagen matrix with epidermal growth factor strongly inhibited wound contraction over matrix alone (p < 0.01 on day 28). Histological analyses showed a regular horizontal arrangement of collagen fibers in the dermis under wounds treated with these substances but not under untreated wounds. Furthermore, using a fibroblast-populated collagen gel, the direct inhibitory effect of epidermal growth factor on gel contraction by fibroblasts was also observed. Collagen gels without stimulation contracted to 29.5 +/- 0. 6% of their original size, as determined 6 days after culturing. At 3 days or more, epidermal growth factor inhibited collagen gel contraction by fibroblasts (after 6 days: 34.2 +/- 1.8%, p > 0.05; 36.5 +/- 2.8%, p < 0.05; and 39.8 +/- 2.1%, p < 0.001 at 1, 10, and 100 ng/ml of epidermal growth factor, respectively). In conclusion, collagen matrix and epidermal growth factor, particularly in combination, may be useful in the prevention of wound contracture.

Animals↗

PubMedDate not supplied

Histopathological changes in the hair follicle after irradiation of long-pulse alexandrite laser equipped with a cooling device.

Several laser systems are currently used for epilation. However, the optimal parameters and treatment protocols remain largely unknown. In this study, the histopathological changes in the hair follicles after long pulsed alexandrite laser irradiation are discussed and the clinical application of irradiation energy at the time of treatment is described. The laser used in this study is equipped with a computer controlled cooling device. A histopathological study was performed to compare the differences in the degree of degeneration in the epidermis and follicles treated with and without the cooling device. In comparison with normal hair papilla, in the laser irradiated skin, injury of melanin containing cells and hair shafts and external root-sheaths in the periphery were observed, while the epidermis was completely protected by the cooling device. The condition of the hair papilla of the tissue irradiated at a fluence of 25 J/cm2 with a spot diameter of 15 mm and 20 J/cm2, with a spot diameter of 18 mm were investigated thoroughly. Swollen papilla and selective destruction of the site where melanocytes were present were observed. The results of this study strongly indicate the efficacy of a long-pulsed alexandrite laser equipped with a computer controlled cooling device for clinical depilation.

Beryllium↗