Biomedical subjects

T Tateno

Publications and source records attributed to T Tateno.

47 records · Page 3Linked to original sources

PubMedDate not supplied

Absence of Y chromosome microdeletions involving the DAZ (deleted in azoospermia) and RBM (ribonucleic acid-binding motif) genes in patients with chordee without hypospadias.

The presence or absence of 33 DNA loci was investigated with a focus on the long arm of Y chromosome (Yq) interval 6 involving the DAZ and RBM genes, by means of a polymerase chain reaction strategy, in 10 patients with hypospadias. All patients did not show any of the 33 DNA loci. It would appear that chordee without hypospadias does not involve interval 6 of the Y chromosome.

Child↗

PubMedDate not supplied

Detection of chromosome 15 deletion in Prader-Willi syndrome using fluorescence in situ hybridization.

Deletion of chromosome 15 was investigated in 6 patients with Prader-Willi syndrome (PWS) using chromosome and fluorescence in situ hybridization (FISH) analysis. Although chromosome analysis using G-banding methods revealed the deletion of 15q11-q13 in only 3 cases, staining by FISH using D15S11 and/or small nuclear ribonucleoprotein polypeptide N (SNRPN) probes detected chromosome 15 deletion in all cases. It would appear that FISH analysis is an effective diagnostic test for the detection of chromosome 15 deletion in patients with PWS.

Adolescent↗

PubMedDate not supplied

Laparoscopic varicocelectomy in adolescents using an ultrasonically activated scalpel.

Ten laparoscopic varicocelectomies were performed using an ultrasonically activated scalpel to divide spermatic vessels in adolescents. The mean operation time was 35 (25-46) min. There were no intraabdominal viscelar or vascular complications, and no postoperative analgesia was required. These findings suggest that laparoscopic varicocelectomy using an ultrasonically activated scalpel may be a valuable treatment in the adolescent.

Adolescent↗

PubMedDate not supplied

Reevaluation of testicular biopsies of males with nonobstructive azoospermia in assisted reproductive technology.

Spermatogenesis was investigated in seminiferous tubules of 100 males with nonobstructive azoospermia. Forty-four (44%) cases had Sertoli-cell-only syndrome, 23 (23%) had spermatogonium in the tubules, 17 (17%) had primary spermatocyte in the tubules, and 16 (16%) had round or late spermatid in the tubules. No cases showed secondary spermatocyte present in the tubules. The mean serum follicle-stimulating hormone (FSH) in males with nonobstructive azoospermia was significantly higher than that in males with obstructive azoospermia (p < .001). The mean concentrations of serum FSH in cases with Sertoli-cell-only syndrome and spermatogonium in the tubules were significantly higher than those in cases with primary spermatocyte and spermatid in the tubules (p < .05-.001). The results indicate that the evaluation of testicular histology using the type of germ cells present in seminiferous tubules is available for assisted reproductive technology.

Biopsy↗

PubMedDate not supplied

Deletion of Y chromosome involving the DAZ (deleted in azoospermia) gene in XX males.

The testicular histology and the presence or absence of 32 Y DNA loci was investigated, with a focus on the long arm of Y chromosome (Yq) interval 6, by means of a polymerase chain reaction strategy in 2 XX males. Seminiferous tubules lined by only Sertoli cells and a slight thickening of tubular walls were observed. The men showed an absence of 32 Y DNA loci. These facts suggest that severe spermatogenic impairment is caused by deletions of Yq interval 6 in XX males.

Adult↗

PubMedDate not supplied

Influence of adenine-induced chronic renal failure on testicular function in the rat.

The influence of adenine-induced chronic renal failure on the male reproductive function was investigated using male Wistar rats. Adenine-induced chronic renal failure was performed in 22 rats (adenine-treated) by providing them with an adenine-excessive diet (150 mg adenine per day) for 10 days. An additional 22 rats were fed without adenine-excessive diet and served as a control group. On days 30 and 60 after start of treatment, body weight ratio to the testis, serum levels of creatinine, urea nitrogen, luteinizing hormone (LH), follicle-stimulating hormone (FSH), 17 alpha hydroxyprogesterone (17 alpha-OHP), androstenedione and testosterone were measured. During the experiment, progressive elevation of serum creatinine and urea nitrogen was found in adenine-treated rats. Body weight ratio of the testes and serum level of LH in adenine-treated rats were similar to those in the controls. On day 60, the FSH level in adenine-treated rats was significantly higher than that in the controls (P < 0.001). While serum concentrations of 17 alpha-OHP and androstenedione in adenine-treated rats were considerably elevated compared with those in the controls on day 30 (P < 0.05). These concentrations did not differ between the two groups at day 60. Serum testosterone level was reduced in adenine-treated rats (P < 0.001). This reduction was progressive with time (P < 0.05). These results suggest that adenine itself reduces testosterone synthesis by suppressing 17 beta-hydroxysteroid oxidoreductase at the early stage of adenine-induced chronic renal failure, but that progressive renal insufficiency causes an elevation of FSH, a reduced synthesis of testosterone and its precursors at the late stage of adenine-induced chronic renal failure. Thus, hormonal conditions in the male rat with adenine-induced chronic renal failure change with time after induction.

17-alpha-Hydroxyprogesterone↗

PubMedDate not supplied

Gonadal function and testicular histology in Noonan's syndrome with bilateral cryptorchidism.

Testicular histology and gonadal function were studied in 2 patients with Noonan's syndrome accompanied by bilateral cryptorchidism. Plasma FSH level was elevated above the normal range, but plasma levels of LH, prolactin, and testosterone were within normal ranges in both cases. The administration of LH-RH resulted in abnormally high response of plasma gonadotropins in both cases. The response of plasma testosterone to the administration of LH-RH was poor in case 2, but case 1 showed normal response. Testicular histology showed reduction of tubular diameter, Leydig cells per seminiferous tubules, and spermatogonia per tubule in both cases. The results indicate that bilateral cryptorchid patients with Noonan's syndrome have an abnormality in the hypothalamo-pituitary-gonadal axis.

Child↗

PubMedDate not supplied

Hormone profiles and testicular histology in cryptorchid boys with marker chromosome.

Hormone profiles and testicular histology were studied in two cryptorchid boys with marker chromosome. Plasma testosterone levels were abnormally low in both cases. Plasma levels of gonadotropins and prolactin were within normal ranges in case 1, but case 2 showed decreased concentration of plasma gonadotropins and normal level of plasma prolactin. The administration of LH-RH induced low response of plasma gonadotropins in case 1 and exaggerated response in case 2. The response of plasma testosterone to the administration of hCG was poor in both cases. Testicular histology showed maturation arrest and reduction of tubular diameter and Leydig cells per seminiferous tubules in both cases. Although testicular histology in these cases were similar to those in prepubertal or pubertal boys with cryptorchidism, hormonal disturbances were more severe. It would appear that marker chromosome enhances hormonal alterations.

Adolescent↗

PubMedDate not supplied

Testicular histology in experimental uremic rats.

Testicular histology in chronic renal failure was studied using stepwise-nephrectomized and adenine-treated rats. Serum levels of urea nitrogen and creatinine were significantly higher than those in sham-operated and control rats (P < .001). However, the mean tubular diameter was not different among the 4 groups. Numbers of A-spermatogonia, preleptotene and pachytene spermatocytes, and spermatids per 100 Sertoli cells were not different among the 4 groups. It would appear that testicular histology is not influenced by chronic renal failure in rats.

Animals↗

PubMedDate not supplied

Circulating levels of inhibin in hemodialysis males.

Serum concentrations of LH, FSH, testosterone, and inhibin were measured in 65 hemodialysis male patients and 60 healthy men. In hemodialysis patients, serum levels of LH, FSH, and inhibin were significantly elevated compared to the controls (p < 0.001). However, serum testosterone level was significantly reduced in hemodialysis males (p < 0.01). Neither serum LH nor FSH correlated with inhibin in the control and hemodialysis males. In hemodialysis patients, serum inhibin showed a positive correlation with serum creatinine (p < 0.05). It would appear that an elevated level of serum inhibin is caused by a decreased metabolic clearance rate or testicular dysfunction due to chronic renal failure, and indicates the abnormality of hypothalamic-pituitary-testicular axis leading to hypergonadotropic hypogonadism in hemodialysis males.

Adult↗

PubMedDate not supplied

Round spermatids from prepubertal mouse testis can develop into normal offspring.

We investigated fertilization and embryo development after intracytoplasmic injection of round spermatids collected from mice before puberty. The rates of normal fertilization, blastocyst formation, and development into live offspring were examined. The injection of oocytes with round spermatids from mature or prepubertal males resulted in similar rates of normal fertilization and embryo development. The rates of the development to term of two-cell embryos were also similar, regardless of the origin of the round spermatids injected. This finding suggests that round spermatids from the prepubertal testis already have the ability to fertilize normally and to allow normal embryo development.

Animals↗