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Biomedical subjects

T Tanabe

Publications and source records attributed to T Tanabe.

820 records · Page 46Linked to original sources
PubMedDate not supplied

Species difference of mitochondrial respiration in experimentally induced heart failure.

In guinea pigs with acute heart failure, a decrease in oxygen consumption (QO2) at state 3 and an increase in QO2 at state 4 were seen and the respiratory control index (RCI) was markedly lowered; ADP/O ratio was not affected. In rabbits with acute heart failure, both RCI and ADP/O ratio showed a statistically significant decrease. In dogs with acute and chronic heart failure, no impairment of respiratory function of mitochondria was seen. These conflicting results may be attributed to the difference in experimental conditions, namely, the species difference and the difference in methods to produce experimental heart failure. With special regard to species difference, it was confirmed that the RCI of myocardial mitochondria of healthy dogs was much lower than that of healthy guinea pigs. It is suggested that the difficulty of obtaining intact mitochondria may be responsible for the species difference in experimentally induced heart failure.

Acute Disease↗
PubMedDate not supplied

Transcatheter placement of an intraluminal prosthesis for the thoracic aorta. A new approach to aortic dissections.

A new aortic prosthesis (SAP, shape memory aortic prosthesis) was developed with its transfemoral placement system to treat aortic dissection with minimal invasion. The SAP consists of a Nitinol stent and polyurethane tube, and it is designed in such a configuration that it can be compressed inside a 14 Fr catheter when cold. It regains its original shape when warmed to 30 degrees C. From the results of intra-aortic sutureless placement of SAP in 10 mongrel dogs, we concluded that a SAP of proper diameter could be implanted safely for more than 3 months. The transfemoral placement system is composed of a delivery catheter, pushing rod, catching catheter, and pulling wire. Using these devices, transfemoral placement of SAP was demonstrated successfully in three dogs and one sheep. The original SAP and delivery system can be used as an emergency procedure for aortic dissections, such as Stanford type B. The advantages of the procedure are minimal invasion of acutely ill patients and prompt application in the catheter laboratory.

Alloys↗
PubMedDate not supplied

Modern trends in the management of aortic aneurysms. Review of 450 cases experienced.

Treatment of various aortic aneurysms in 450 cases experienced during the past 25 years was analysed. One hundred and five patients were treated with survival rate of 80.2% in the thoracic aortic aneurysm group, 65 patients with survival rate of 76.9% in the dissected aneurysm of the aorta group, 175 patients with survival rate of 93.1% in the nonruptured abdominal aortic aneurysm group and 37 patients with survival rate of 75.7% in the ruptured abdominal aortic aneurysm group. From 1981 to the present time, considerable improvement of the operative results has been achieved with refinement of cardiovascular adjunctive techniques and operative procedures with careful management of the patients. Survival rates are now reached to 88.7% in the thoracic aortic aneurysm, 81.0% in the dissected aneurysm, 100% in the nonruptured abdominal aortic aneurysm and 81.2% in the ruptured abdominal aortic aneurysm. From the careful analyses of the results, prompt diagnosis, distinct treatment policy, strict operative indication and better surgical procedure are considered to have contributed to these excellent operative results.

Adult↗
PubMedDate not supplied

Patch angioplasty of the superior vena caval obstruction (case reports with long follow-up results).

Four cases of the superior vena caval obstruction caused by mediastinal tumor and fibrous mediastinitis are presented. Palliation was achieved by resection of tumor or scar tissue and placing a Teflon or autogenous vein patch to the resected defect of the superior vena cava. One of the patients died of metastasis at 8 months postoperatively, but patency of the patch graft was confirmed. The other three are alive and well at 14, 14 and 11 years, respectively. Continued patency has been confirmed by venographies in two of them.

Adult↗
PubMedDate not supplied

In vitro anti-invasive effects of N-(4-hydroxyphenyl)-retinamide on human prostatic adenocarcinoma.

Components of malignant invasion, namely cellular adhesion, motility, and proteolytic capability provide potential sites of pharmacological intervention for malignancy. In this study, a series of experiments were performed to examine the effects of N-(4-hydroxyphenyl) retinamide (4-HPR, Fenretinide) on cellular adhesion, motility and proteolytic activity of established prostate cancer cell lines, TSU-PR 1 and PC-3. Radioadhesion study showed that the treatment of TSU-PR 1 and PC-3 cells with 10(-6) M of 4-HPR resulted in a 32% and 37% reduction (p < 0.05), respectively, in the cellular adhesion to the matrigel extract. Radiomigration assay also demonstrated that 4-HPR concentration of 10(-6) M reduced the cellular motility by 29% in TSU-PR1 and 28% in PC-3 cells (p < 0.05). Spectrolyse PL indirect chromogenic assay revealed an increase in total activatable uPA activity (TSU-PR 1: 25%, PC-3: 32%, P < 0.05), while Spectrolyse UK direct assay demonstrated a mild, but a statistically significant reduction (PC-3: 5%, TSU-PR1: 9%, P < 0.05) in active uPA activity. Northern analysis and ELISA assays showed that 4-HPR at 10(-6) M enhances the expression of type 1 plasminogen activator inhibitor (PAI-1). Type IV collagenase western blot analysis and densitometry did not demonstrate suppression of the enzyme secretion, but in fact suggested increased translation of the enzyme when treated with 10(-6) M concentration of fenretinide. The results of this study demonstrate that 4-HPR inhibits in vitro cellular adhesion and motility of human prostate adenocarcinoma cell lines, TSU-PR1 and PC-3. Additionally, uPA and PAI-1 assay results suggest that 4-HPR may impair active uPA's proteolytic activity while upregulating the expression of total activatable uPA and PAI-1. The results of this study therefore support 4-HPR's role as a potential anti-invasive agent.

Adenocarcinoma↗
PubMedDate not supplied

Superiority of interleukin-12-transduced murine lung cancer cells to GM-CSF or B7-1 (CD80) transfectants for therapeutic antitumor immunity in syngeneic immunocompetent mice.

Interleukin-12 (IL-12) is a heterodimeric cytokine that consists of p40 and p35 subunits. IL-12 has been regarded as a potent inducer of host antitumor immunity through interferon-gamma (IFN-gamma) production and development of Th1 helper T cells from Th0 cells. Here, we demonstrate the immunomodulatory actions of an IL-12-transduced murine lung cancer cell line, Lewis lung carcinoma (LLC) (LLC/IL12) cells, in syngeneic C57BL/6 mice. We also report on their therapeutic potency. Three LLC/IL12 cells producing different levels of IL-12 were cloned and found to have diminished tumorigenicity in C57BL/6 mice depending on their level of IL-12 production. In vivo depletion assay demonstrated that the loss of tumorigenicity of LLC/IL12 depended on both CD4+ and CD8+ T cells, and that natural killer (NK) cells were involved, especially in the early phase of immunity. The strong systemic antitumor immunity against challenge with wild type LLC (LLC/wt) cells was also induced by LLC/IL12 cells. The systemic antitumor memory was found to be dependent mainly on the CD4+ T-cell subset. 51Cr-release assay revealed that the killer activity consisted of a specific killer activity directed at the parental LLC/wt cells and a nonspecific killer activity directed at both LLC/wt and syngeneic EL-4 thymoma cells. In addition, LLC/IL12 apparently had a much stronger antitumor effect against the established LLC/wt tumor than LLC transduced with B7-1 or GM-CSF cDNA. IL-12 can be considered an efficient candidate molecule for immunogene therapy for lung cancer in this experimental system.

Animals↗