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Biomedical subjects

T Takiguchi

Publications and source records attributed to T Takiguchi.

At least 109 records · Page 6Linked to original sources
PubMed1980

The paradoxical effects of thioglycollate-induced macrophages on the growth of B16 melanoma cells in vivo and in vitro.

The effect of thioglycollate-induced macrophages on the growth of syngeneic B16 melanoma cells was studied. Thioglycollate-induced macrophages showed strong cytostasis and cytotoxicity towards B16 tumor cells in vitro. However, these effects were not demonstrable in an in vivo assay which involved local transfer and experimental metastasis. In contrast to the in vitro results, mice injected iv with both B16 tumor cells alone. Possible reasons for the failure of thioglycollate-induced macrophages to exhibit cytostatic and cytotoxic activity in vivo are discussed.

Animals↗
PubMed1979

Effects of deep hypothermia and circulatory arrest on the auditory brain stem responses.

The effects of hypothermia on auditory brain stem response (ABR) in both children with congenital heart disease undergoing cardiac surgery and cats as an animal model were investigated. The latency of the ABR waves were prolonged with decreased body temperature. The latency of the later response components were prolonged more than that of the earlier response components; all waves disappeared below 25 degrees C and during artificial cardiac arrest. When the body temperature was raised, the later component began to reappear above 25 degrees C; the latency of all components shortened with temperature until normal responses were obtained at 34 degrees C. The data from both humans and cats were comparable. The ABR seems to be a useful monitor for evaluating brain stem function during deep hypothermia.

Animals↗
PubMed1977

T cell function detected in murine bone marrow cells.

Mouse bone marrow cells were fractionated by BSA discontinuous density gradient centrifugation, and a small lymphocyte rich fraction was obtained at the high density. Cells of this fraction were shown to respond in vitro to T cell mitogens and alloantigens. Furthermore, they were able to mount a graft-versus-host reaction when assessed by spleen weight assay and by the method of inhibiting erythroid cell growth by allogeneic lymphoid cells. The results indicate that these lymphocytes possess T cell functions. On the other hand they were found to carry only little theta antigen assessed by the cytotoxic test and by the absorption test. It is presumed, therefore, that the amount of theta antigen on a cell might not correlate with T cell functions, and these lymphocytes might be mature ones in the course of postthymic maturation. Hemopoietic stem cells were determined by spleen colony formation and the peak of colony-forming efficiency was seen at the low density. These observations imply that immunocompetent cells causing GVHR can be separated from hemopoietic stem cells. This procedure may be applied for prevention and reduction of GVHR in allogeneic bone marrow transplantation in human.

Animals↗
PubMed1971

Cellular recognition in vitro by mouse lymphocytes. Effects of neonatal thymectomy and thymus graft restoration on alloantigen and PHA stimulation of whole and gradient-separated subpopulations of spleen cells.

The effects of thymectomy and thymus graft restoration upon the in vitro primary responses to alloantigens and PHA have been studied. It has been found that neonatal thymectomy substantially eliminates both PHA reactivity and responsiveness to alloantigens assayed in vitro in host spleen cell populations. Analysis of albumin density gradient-separated subpopulations of the spleen and thymus in such animals was also performed. It was found that the total and proportional representation of the individual density subpopulations was identical in neonatally thymectomized, in normal, and in thymectomized and thymus graft-restored animals. Therefore, thymectomized mice appear to retain a nonfunctioning, small, dense, lymphocyte population. Reconstitution of thymic-dependent in vitro reactivity was nearly complete when syngeneic, but not allogeneic or semisyngeneic thymus was employed. Occasional partial restoration did occur when F(1) thymus was employed, but never when allogeneic thymus was grafted. The grafted thymus contained PHA and alloantigen-reactive cells in a large, less dense B layer subpopulation, whereas the restored animals, as in the case of normals, showed these reactivities to be a property of a small, more dense cell population.

Albumins↗