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Biomedical subjects

T T Puck

Publications and source records attributed to T T Puck.

At least 109 records · Page 6Linked to original sources
PubMed1969

Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants.

Complementation analysis at the single gene level has been carried out on 13 different, recessive, glycine-requiring mutants produced by action of mutagenic agents on the CHO-K1 Chinese hamster cell. The mutants form four different complementation classes indicating that at least four separate genes can produce a growth requirement for glycine. The four classes of mutants can be distinguished biochemically as well as genetically. Existence of these four mutant classes appears to explain the preponderance of the glycine-requiring forms in the auxotrophs produced by treatment of the parental cell with mutagenic agents.

Animals↗
PubMed1966

Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin.

Analysis of the effects of actinomycin and puromycin on the G(2) and mitotic parts of the life cycle in Chinese hamster ovary cells grown in suspension and synchronized by thymidine treatment has been carried out. Rates of division of partially synchronized cell populations were measured in the presence and absence of the drugs, and various controls were performed to test for absence of complex side effects. Actinomycin produces a block 1.9 hr before completion of division, while puromycin produces a block almost coinciding with the initiation of mitosis. Evidence is presented that the puromycin block may be a double one, inhibiting one kind of protein synthesis that virtually coincides with the beginning of mitosis and another that occurs about 8 min earlier. The data are interpreted in terms of the time interval between messenger formation and its associated protein synthesis in this region of the life cycle. The various events studied have been provisionally mapped in the G(2) and mitotic periods of the life cycle.

Animals↗