Hepatitis C virus infection in acute leukemia with liver dysfunction.
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Acute Disease↗
Biomedical subjects
T Suou
Publications and source records attributed to T Suou.
Leukocyte sensitivity to native human interstitial collagen in chronic liver disease.
Peripheral blood mononuclear cells and lymphocyte subsets separated from 28 patients with chronic liver diseases, and 11 age and sex matched normal controls were examined for cellular sensitivity to native human interstitial collagens and phytohemagglutinin, non-specific mitogen, by an in vitro leukocyte migration inhibitory test. The leukocyte sensitivity of peripheral blood mononuclear cells to type I, III, and V collagens was significantly higher in patients with chronic liver disease (26 +/- 10%, 25 +/- 11%, and 24 +/- 10%, respectively) than in controls (9 +/- 4%, 10 +/- 6%, and 9 +/- 3%, respectively, p < 0.001). Although the leukocyte sensitivity to phytohemagglutinin was significantly lower in patients with chronic liver disease than in controls (52 +/- 7% vs. 62 +/- 7%, p < 0.01). The leukocyte sensitivity to interstitial collagen in chronic liver disease was neither type specific nor disease specific, but it was correlated with the degree of hepatic fibrosis, or serum concentrations of procollagen type III peptide and 7S domain of type IV collagen. On the other hand, among subsets, the leukocyte sensitivity of T cells was significantly higher than that of B cells (p < 0.01), although there was no difference in leukocyte sensitivity between CD4 and CD8 T cells. These findings demonstrate that cellular immunity to interstitial collagen increases in chronic liver disease, reflecting the increased metabolism of hepatic collagen, in contrast to the pattern of decreased immunity to the non-specific mitogen.
Adult↗
Cellular sensitivity to native type IV collagen in primary biliary cirrhosis.
We examined 60 patients with various chronic liver diseases for cellular sensitivity to native human type I and IV collagens using an in vitro leucocyte migration inhibition test. Mononuclear cells from 7 (33%) of 21 patients with chronic active hepatitis, 14 (52%) of 27 patients with liver cirrhosis and 11 (92%) of 12 patients with primary biliary cirrhosis exhibited cellular sensitivity to type IV collagen, although cells from almost all patients responded to type I collagen. None except one for type I collagen of 25 normal controls showed positive response to both collagens. In chronic active hepatitis and liver cirrhosis, cellular sensitivity to type IV collagen was significantly lower than to type I collagen (p < 0.01). Patients with primary biliary cirrhosis showed significantly higher cellular sensitivity to type IV collagen when compared to patients with other chronic liver diseases (p < 0.01). Cellular sensitivity to type IV collagen was significantly correlated with serum levels of the 7S domain of type IV collagen in all 85 subjects (r = +0.462, p < 0.001). These findings suggest that cellular sensitivity to type IV collagen as well as to type I collagen exists in chronic liver disease, especially in primary biliary cirrhosis, and may reflect the accelerated metabolism of the perisinusoidal and peribiliary basement membranes.
Adult↗
Heterogeneity of antigens related to 7S domain of type IV collagen in serum: application to patients with chronic liver disease.
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Antibody Specificity↗
Clinical significance of serum 7S collagen in various liver diseases.
Serum type IV collagen fragment (7S collagen domain) was measured in 30 controls and 152 liver disease patients with a radioimmunoassay using a polyclonal antibody to human placenta 7S collagen. The serum concentrations of 7S collagen (mean +/- SD) were 4.2 +/- 0.9 ng/mL in controls, 5.1 +/- 2.0 ng/mL in acute hepatitis, 6.5 +/- 2.5 ng/mL in chronic inactive hepatitis, 9.5 +/- 3.8 ng/mL in chronic active hepatitis, 14.4 +/- 7.5 ng/mL in liver cirrhosis, and 14.4 +/- 6.9 ng/mL in hepatocellular carcinoma. In acute hepatitis, 7S collagen was slightly increased, whereas type III procollagen N-peptide and prolyl hydroxylase were markedly increased. In chronic liver disease, 7S collagen concentrations increased with the severity of the disease, and also reflected the degree of fibrosis. The serum 7S collagen concentrations were significantly correlated with those of type III procollagen N-peptide and prolyl hydroxylase in all subjects. These results suggest that serum 7S collagen concentration is a useful diagnostic aid for determining hepatic collagen metabolism in liver diseases.
Adult↗
Clinical significance of serum and urinary neopterin levels in patients with various liver diseases.
Serum and urinary neopterin levels were measured by radioimmunoassay in 120 healthy controls, 16 asymptomatic HBsAg carriers, 12 patients with acute hepatitis, 13 with chronic inactive hepatitis, 35 with chronic active hepatitis, 46 with liver cirrhosis, 18 with hepatocellular carcinoma, and 6 with alcoholic liver disease. Serum and urinary neopterin levels were significantly higher in almost all patients than in normal subjects. Neopterin levels were highest in acute hepatitis and correlated with the results of liver function tests, but did not show this correlation in chronic liver disease. In chronic liver disease, the levels of serum neopterin in non-A non-B viral patients was significantly increased, compared with those in B viral and alcoholic patients. The rate of abnormal urinary neopterin levels in chronic liver disease was higher than the rate of abnormal serum neopterin levels, but no difference was observed between the rates of abnormal serum and urinary levels in acute hepatitis and asymptomatic HBsAg carriers. These results indicate that serum and urinary neopterin levels may be useful markers for cell-mediated immunity in liver disease, and that the immune system response in chronic liver disease may be different for different pathogens.
Adolescent↗
A simplified gelatin tolerance test to evaluate gastric and pancreatic proteolytic activities.
To evaluate gastric, pancreatic and intestinal proteolytic activities, a gelatin tolerance test was standardized, and its clinical significance was evaluated in 16 normal subjects and 25 patients who had undergone gastrectomy or had a pancreatic disease. A 15g gelatin dose given to normal subjects resulted in an increase of serum-free and peptide-bound hydroxyproline (HYP), with the peak values being observed at 1 hr and 2 hr, respectively. In patients who had malabsorption, both peak levels of serum-free and peptide-bound HYP were decreased, and the peak value of free HYP was more frequently lower than that of peptide-bound HYP. The determination of serum free HYP after oral administration og gelatin was significantly correlated with BT-PABA test. These results indicate that usefulness of determining serum-free HYP for the gelatin tolerance test as a simple reliable test for digestion and absorption of proteins.
Administration, Oral↗
Clinical significance of benzoate-metabolizing capacity in patients with chronic liver disease: pharmacokinetic analysis.
Benzoate-metabolizing capacity was studied in control subjects and in liver disease patients after intra-venous loading of 15 mg benzoate per kg of body weight. In the 7 control subjects, the mean level (+/- SEM) of Cmax for serum benzoate was 104.1 +/- 6.8 micrograms/ml, AUC was 2.57 +/- 0.32 mg.min/ml, MRT was 21.5 +/- 1.5 min and T1/2 was 15.5 +/- 1.3 min. For serum hippurate, on the other hand, Tmax was 27.9 +/- 6.0 min, Cmax was 33.4 +/- 2.1 micrograms/ml, AUC was 1.96 +/- 0.13 mg.min/ml, MRT was 39.6 +/- 2.9 min and T1/2 was 30.7 +/- 2.4 min. In 12 patients with chronic hepatitis, Cmax, AUC, MRT and T1/2 for benzoate and Tmax, MRT and T1/2 for hippurate remained at control levels, but Cmax and AUC for hippurate were slightly decreased compared to controls. However, in 18 patients with liver cirrhosis, Cmax and AUC for benzoate were in the control range but MRT and T1/2 were significantly delayed (p less than 0.01 for both). Moreover, the MRT value was increased in proportion to the severity of liver disease (p less than 0.01). AUC for hippurate was not changed to any extent, and Tmax, MRT and T1/2 were slightly delayed, while Cmax was significantly reduced. AUC, MRT and T1/2 for benzoate and Tmax, MRT and T1/2 for hippurate showed significant correlation with serum albumin levels, prothrombin time and indocyanine green clearance rate. These results suggest that benzoate-metabolizing capacity, especially as indicated by the MRT value for serum benzoate, appears to be a better index than the indocyanine green clearance rate for determining hepatic functional reserve in chronic liver disease.
Adult↗
[Prevalence of HCV antibodies in Yatsuka town of Simane prefecture, Japan].
To clarify the potential role for HCV in the development of chronic liver disease in Yatsuka town with a high morbidity of liver disease, epidemiological studies were taken in 459 subjects of Yatsuka town compared with 219 subjects of Mihonoseki town with a low one. In Yatsuka town, the mortality rate of liver cirrhosis was three times higher than the overall rate in Simane prefecture, and the rate of liver cancer rapidly increased in recent years. Age and sex-matched epidemiological studies showed a significantly higher incidence of hepatic dysfunction compared with Mihonoseki town (11.5% vs 3.7%, P less than 0.01). The prevalence of HCV antibody was significantly higher in Yatsuka town than Mihonoseki town (16.6% vs 3.7%, P less than 0.001), although there were no differences in frequencies of HBs antigen and habitual alcohol drinker between the two districts. These data strongly suggest that the high prevalence of HCV may be associated with the high morbidity of chronic liver disease, especially hepatocellular carcinoma in Yatsuka town.
Adult↗
Defective immunoregulation in primary biliary cirrhosis: CD4+, Leu-8+ T cells have abnormal activation and suppressor function in vitro.
To determine whether abnormalities of lymphocyte function in primary biliary cirrhosis are due to altered function of immunoregulatory T cell subpopulations, phenotypic and functional characteristics of CD4+ T cells were examined. The proportion of CD4+ T cells expressing the Leu-8 and CD45R antigens was normal in patients with primary biliary cirrhosis. The capacity of CD4+, Leu-8- T cells to provide helper function for pokeweed mitogen-stimulated immunoglobulin synthesis by B cells in vitro was similar in patients and controls. However, in contrast to normal individuals and patients with other liver diseases, CD4+, Leu-8+ T cells from six of 10 patients with primary biliary cirrhosis did not suppress, but enhanced immunoglobulin synthesis. Whereas treatment of CD4+ T cells from normal individuals with anti-Leu-8 monoclonal antibody enhanced their suppressor function, similar treatment of CD4+ T cells from patients with primary biliary cirrhosis did not increase their suppressor function. To determine whether the abnormal regulatory function of CD4+, Leu-8+ T cells was due to a defect of cell activation, the proliferative response of CD4+ T cell subpopulations to mitogenic stimulation was examined. The proliferative responses of CD4+, Leu-8- T cells from patients with primary biliary cirrhosis and controls were similar, but the proliferative responses of CD4+, Leu-8+ T cells from patients with primary biliary cirrhosis were lower than those of control cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Adult↗
Urinary hydroxyproline and hydroxylysine excretions in relation to hepatic hydroxyproline content in chronic liver disease.
In patients with or without various chronic liver diseases, the total urinary excretion of hydroxyproline and hydroxylysine and the hepatic content of hydroxyproline were examined. In 7 patients without liver disease, the urinary excretion of hydroxyproline and hydroxylysine were 10.3 +/- 1.5 and 1.31 +/- 0.21 mmol/mol creatinine, respectively, and the hepatic content of hydroxyproline was 4.9 +/- 0.6 mumol/g of wet liver. In 33 patients with liver disease, the urinary excretion of hydroxyproline and hydroxylysine and the hepatic content of hydroxyproline were increased in proportion to the severity of liver disease. The hepatic content of hydroxyproline showed a significant correlation with the urinary excretion of hydroxyproline and hydroxylysine (r = +0.406 and r = +0.531, respectively). These results suggest that the study of urinary hydroxyproline and hydroxylysine excretion may yield useful information on the metabolism of hepatic collagen in chronic liver disease. Moreover, urinary hydroxylysine excretion seemed to be a better index of hepatic collagen metabolism than urinary hydroxyproline excretion; perhaps urinary hydroxylysine excretion is not much affected by dietary collagen intake.
Adult↗
Oral contraceptives and intrahepatic biliary cystadenoma having an increased level of estrogen receptor.
We report a case of intrahepatic biliary cystadenoma in a young woman who had no prior history of liver disease and who had taken oral contraceptives for one year. A 27-year-old woman was admitted to our hospital with a firm epigastric mass. At laparotomy, a large cystic mass was resected from the left hepatic lobe, and diagnosed as biliary cystadenoma. In the tumor tissue, the estrogen receptor content was 14.0 fmol/mg cytosol protein, which was much higher than the 3.0 fmol/mg cytosol protein in the surrounding liver tissue. This is the first case of biliary cystadenoma in which the estrogen receptor content was increased.
Adult↗
Hepatotoxicity of cyclophosphamide in man: pharmacokinetic analysis.
Of 44 patients with neoplasia treated with cyclophosphamide (CPA), 19 (43%) had a transient elevation of serum levels of aminotransferases. In these patients, regardless of the combination chemotherapy regimen given, the incidence of liver dysfunction was 33%, when the total CPA dose was less than 400 mg/m2 (Group A) and 73% with higher doses (Group B). Prior to the initiation of CPA treatment, pharmacokinetics of CPA were investigated in 15 patients. Plasma AUCs of CPA and phosphoramide mustard (PM) in 8 patients in Group B were higher than those in 7 Group A patients. In contrast, urinary excretion of 3-hydroxypropyl-mercapturic acid (3-HPMCA) was lower in Group B than in Group A. The ratio of urinary 3-HPMCA to plasma AUC of PM was significantly lower in Group B than in Group A (p less than 0.05). These results indicate that CPA-induced liver injury is mainly dose-dependent and presumably results from impaired metabolism of CPA, and especially of its metabolite, acrolein.
Adolescent↗
Hepatocellular carcinoma metastatic to the oral cavity including the maxilla and the mandible: report of two cases and review of the literature.
Two cases of hepatocellular carcinoma metastatic to the oral cavity are presented. One patient had metastases to the maxilla and finally, to the mandible, and the other patient, to the mandible. Both cases histologically showed highly-differentiated trabecular hepatocellular carcinoma which had vascularized stroma, explaining the frequently observed oral hemorrhage. The clinical signs and symptoms described here suggested the existence of a tumor metastatic to the oral cavity, and might indicate an unusual manifestation of hepatocellular carcinoma. Reports of metastatic lesions of hepatocellular carcinoma to the oral cavity, including the mandible, maxilla and gingiva proper, are reviewed.
Aged↗
Cellular sensitivity to collagen in liver disease.
Peripheral mononuclear cells from 29 patients with liver disease and from 15 normal subjects were examined for cellular sensitivity to purified bovine type I and type II collagens by the in vitro production of leucocyte migration inhibitory factor. Mononuclear cells from 21 (69%) of the patients responded to type I collagen, but cells from only one (3%) did to type II collagen. In normal subjects, there were no response to both collagens. As compared with the corresponding humoral antibody response, the cellular sensitivity was more specific for type I collagen in patients with liver disease. These data demonstrate that cellular sensitivity to type I collagen exists in liver disease, presumably in relation to hepatic fibrosis.
Adolescent↗
[Clinical studies of cefotaxime in infections in internal medicine].
Clinical studies on a new cephalosporin antibiotic, cefotaxime (CTX) were carried out in 79 patients with various types of infections in internal medicine. The efficacy rates were 65.8% in 39 cases of respiratory tract infections, 94.1% in 17 cases of biliary tract infections, 75.0% in 12 cases of urinary tract infections, 80.0% in 11 cases of other infections, and 75.3% in all cases. After CTX therapy, body temperature was improved in 76.7% of all 79 patients, particularly being excellent in all cases of biliary tract infections. Furthermore, symptoms such as cough, dyspnea, chest pain, moist rale and anorexia were improved to a great degree after CTX therapy. Adverse reactions and abnormal laboratory findings consisted of mild liver injury in 1 out of 79 cases. CTX was assessed to be an effective antibiotic for various types of infections in internal medicine.
Adult↗
Pedunculated hepatocellular carcinoma. Report of three cases and review of literature.
Pedunculated hepatocellular carcinoma (HC) is rarely found in the United States and Europe. There have been several cases in Japan. The diagnosis of pedunculated HC is said to be very difficult. This article describes three cases of pedunculated HC which were diagnosed preoperatively and were resected successfully. Celiac angiography and CT scan were useful diagnostic procedures. World literature is reviewed, and 15 documented cases of pedunculated HC have been found, including the three cases presented. It may be assumed that pedunculated HC may arise from the accessory lobe of the liver or ectopic liver tissue.
Adult↗
Anti-albumin antibodies in sera of patients with liver disease.
We used passive haemagglutination assay to examine sera from 77 patients with various liver diseases for the presence of antibodies to human serum albumin (HSA), bovine serum albumin (BSA) ans ovalbumin (OA). The antibody titre to BSA was higher than that to OA or HSA. Of the examined diseases, in chronic active hepatitis and liver cirrhosis, the titre and incidence of anti-BSA antibody were highest. There was a positive correlation between anti-BSA antibody and serum gamma-globulin level. Sephadex G-200 column chromatography and immunoelectrophoresis revealed that the anti-BSA antibody belonged to the IgM class.
Adult↗