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Biomedical subjects

T Sugimura

Publications and source records attributed to T Sugimura.

At least 919 records · Page 51Linked to original sources
PubMed1978

Mutagenicity of several classes of antitumor agents to Salmonella typhimurium TA98, TA100, and TA92.

The mutagenic activities of antitumor agents, including 5 antibiotics, 19 antimetabolites, 5 alkylating agents, 2 alkaloids, 1 enzyme, and 1 adrenal steroid hormone, were tested on Salmonella tyhimurium TA100, TA98, and TA92. Four of these, busulfan, carbazilquinone, 1-(4-amino-2-methylpyrimidine-5-yl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride, and pipobroman were shown for the first time to be mutagenic. Further, the known mutagenicities of five others, daunomycin hydrochloride, Adriamycin hydrochloride, mitomycin C, 6-mercaptopurine, and cyclophosphamide, were confirmed.

Alkylating Agents↗
PubMed1978

Comparative studies on antibody and antibody production to poly(ADP-ribose) in mice.

Antibodies to poly(ADP-ribose) were produced in C3H/He mice by injection of poly(ADP-ribose) in Freund's incomplete adjuvant or its complexes with methylated bovine serum albumin in Freund's incomplete adjuvant. Titres of the antibody obtained from the latter were about 50-fold higher than those from the former. Thus, the effect of methylated bovine serum albumin on the antibody production to poly(ADP-ribose) resembled the case of poly(I).poly(C) and was different from the case of single stranded DNA. The class of the antibodies obtained from these two different procedures mainly consists of 7S antibodies, and the specificity of these antibodies was independent of their titres. It was also found that antibodies to poly(ADP-ribose) were most reactive to poly(ADP-ribose) with 20 repeating ADP-ribose units. The reactivity of the antibody was dependent on the chain length of the polymer. Neither the complex in Freund's incomplete adjuvant nor poly(ADP-ribose) alone in Freund's imcomplete adjuvant could induce an immune response to poly(ADP-ribose) in athymic nude mice. Therefore, the immune response to poly(ADP-ribose) may occur through a thymus function.

Animals↗
PubMed1978

A single radial hemolysis technique for the measurement of influenza virus antibody in swine serum.

A single radial hemolysis technique was applied to the measurement of swine influenza virus antibody in swine serum. It was recognized to be useful for the detection of this antibody, although heating at 56 degrees C for 30 minutes was necessary to remove non--specific hemolysis from the test sample. The single radial hemolysis titer was correlative with the hemagglutination--inhibition titer in serum from pigs infected experimentally or naturally. This technique is simple, rapid and unaffected with non--specific inhibitors in swine serum. It does not require concentrated purified virus either. Therefore, it is applicable to the sero--epizootiologic surveillance of swine influenza on a large scale.

Animals↗
PubMed1977

Phosphodiesterase from cultured tobacco cells. Physical and chemical properties.

Phosphodiesterase isolated from suspension cultures of tobacco cells showed high affinity for concanavalin A-Sepharose and gave single superimposed bands of protein and carbohydrates on disc gel electrophoresis, suggesting that it is a glycoprotein. It contains 14% carbohydrate by weight, and has relatively high contents of basic and aromatic amino acids. Its isoelectric point is at pH 8.8, and the molecular weight of its subunits was estimated as 72 000 from a plot of the retardation coefficient on sodium dodecyl sulfate gel electrophoresis versus the molecular weight. The enzyme was catalytically active in an immobilized state on a concanavalin A-Sepharose column.

Amino Acids↗
PubMed1977

Effects of various hormones and adrenalectomy on the levels of amylase in rat pancreas and parotid gland.

Dexamethasone, adrenocorticotropic hormone and thyroxine increased the amylase activities in both the pancreas and the parotid gland of infant rats. After adrenalectomy, the amylase activities of the pancreas and parotid gland were about half the control levels, suggesting that both glucocorticoid and thyroxine are involved in maintaining the amylase activities in these organs.

Adrenalectomy↗
PubMed1977

Effects of albumin and other proteins during assay of amylase activity.

Addition of albumin, gamma-globulin, alpha-casein or submaxillary mucin to the assay system for chromogenic measurement of human or rat amylase with blue starch increased the amylase activity, albumin having the most effect. These proteins seemed to increase the activity by protecting amylase from inactivation. Amylase activity was higher in urine samples showing proteinuria than in urine samples without detectable protein. It is concluded that amylase assay is more reliable when a final concentration of albumin of 1 mg per ml is added at the dilution step and at the incubation step.

Albumins↗
PubMed1977

Immunoreactivities of alpha-amylase of humans and rats.

The immunoreactivities of amylase from human saliva and pancreatic juice and rat parotid and pancreas were investigated. Antisera were prepared in rabbits against each of the human and rat amylase. Human salivary and pancreatic amylases reacted similarly with the antibodies to both human salivary and pancreatic amylases. Rat parotid and pancreatic amylases reacted differently with the antibodies to both rat parotid and pancreatic amylases.

Amylases↗
PubMed1977

Perinatal changes in amylase and serum corticosterone levels in rats.

In rats amylase activity in the pancreas increased greatly from day 15 of gestation to a maximum on day 21. Then it decreased to less than one-tenth of this maximum value on about day 5 after birth. It increased again about 15 days after birth and reached the adult level about 30 days after birth. No amylase activity was in the parotid gland before birth: it appeared about 12 days after birth and reached the adult level, which was higher than that in the pancreas, about 30 days after birth. The serum corticosterone level was as high as the adult level before birth. Then it decreased to less than one-tenth of the adult level 5 days after birth and increased again from 15 to 25 days after birth to the adult level. The developmental change in the serum corticosterone level seemed to influence amylase activity in the pancreas both before and after birth, and that in the parotid gland only after birth. The serum contained both pancreatic and parotid type isozymes of amylase until 1 day after birth but only the parotid type from 3 days after birth.

Aging↗
PubMed1977

Blood group activity of human sucrase from intestinal metaplasia.

Sucrases were purified from human small intestine and from areas of intestinal metaplasia of the stomach mucosa surrounding stomach cancers. The kinetic constants and pH activity profiles of enzyme preparations from the two sources were similar. No blood group activity of sucrase was detectable in preparations from three cases of intestinal metaplasia, but preparations from two other cases showed activity like that of the small intestine. These results indicate that sucrase from areas of intestinal metaplasia has similar enzymatic properties to those of enzyme from the small intestine, but that the antigenic sugar moiety of the enzyme associated with blood group activity varies.

ABO Blood-Group System↗
PubMed1977

Carcinogenicity of a food additive, AF-2, in hamsters and mice.

The carcinogenicity of a food additive, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (trade name, AF-2), was studied. A diet containing AF-2 at a rate of 0.25% was administered to male golden hamsters and male ddY mice. The hamsters developed squamous cell carcinomas in the forestomach after the 49th day from the start of administration. In mice fed a AF-2 diet for 308 days, squamous cell carcinoma was observed in the forestomach after the 381th day from the start of the experiment; in some cases the carcinoma metastasized to the lung and liver.

Animals↗
PubMed1977

Induction of liver tumors in rats by sodium nitrite and methylguanidine.

The carcinogenicity of sodium nitrite and methylguanidine singly and together were examined in rats. A hepatocellular carcinoma, a hemangiosarcoma and a spindle cell sarcoma were found in 3 of 15 rats fed continuously on pellet diet containing 0.16% sodium nitrite and 0.16% methylguanidine. Hemangiomas and bile duct adenomas of the liver were also found in 6 and 8, respectively, of the 15 rats in this group. Hemangiomas and bile ducts adenomas of the liver were found in 2 and 3, respectively, of the 4 rats fed on pellet diet containing 0.16% sodium nitrite. Only 1 of 5 rats fed on pellet diet containing 0.16% methylguanidine developed a hemangioma. No tumor was found in the control group. All the tumors were found in rats that survived for over 12 months. No significant changes were detected in the esophagus or stomach.

Adenoma, Bile Duct↗
PubMed1977

Mutagenicities of N-nitrosamines on Salmonella.

The mutagenic activities of 11 N-nitrosamines were tested using Salmonella typhimurium TA100 and TA98. All the carcinogenic N-nitrosamines were mutagenic on TA100 with a drug-activating system from the rat liver, whereas N,N-diphenylnitrosamine, a non-carcinogen, was not mutagenic. None of the N-nitrosamines was mutagenic on TA98, except N,N-diethylnitrosamine which was weakly mutagenic. To detect the mutagenicity of N,N-dimethylnitrosamine, the pre-incubation of bacteria and N,N-dimethylnitrosamine with S-9 Mix before if was poured onto plates was obligatorily required. Dimethyl sulfoxide inhibited the mutagenic effect of N,N-dimethylnitrosamine.

Animals↗
PubMed1977

Mutagenicities of quinoline and its derivatives.

Quinoline, recently reported to be carcinogenic in rats [12], was mutagenic to Salmonella typhimurium tester strains TA100 and TA98 in the presence of the metabolic activation system S-9 mix. 2-Chloroquinoline, a non-carcinogen [12], was non-mutagenic with or without S-9 mix. 8-Hydroxyquinoline, which is t known to be carcinogenic, was mutagenic with S-9 mix to both bacterial strains. The mutagenicities of 17 other quinoline derivatives that are not known to be carcinogenic were tested, and 12 of these compounds were mutagenic.

Animals↗
PubMed1977

Mutagenicities of smoke condensates and the charred surface of fish and meat.

Smoke condensates obtained from broiling fish showed mutagenic activity for Salmonella typhimurium TA100 and TA98. Metabolic activation was required to induce mutagenic activity of smoke condensates of some species of fish. The smoke condensate obtained during charcoal broiling of beefsteak was far less mutagenic than that of fish, with or without metabolic activation. Extracts of the charred surface of broiled fish and meat also contained mutagenic substances. These extracts needed metabolic activation to exhibit mutagenicities on TA98. The mutagenic activity of the smoke condensate obtained from one sardine weighing 100 g was equivalent to that of 132 micrograms benzo(a)pyrene and that of the charred surface of the sardine was equivalent to 358 micrograms benzo(a)pyrene. One piece of beefsteak weighing 190 g, contained mutagenic activity equivalent to that of 855 micrograns benzo(a)pyrene.

Animals↗
PubMed1977

Mutagenicity of smoke condensates from cigarettes, cigars and pipe tobacco.

Smoke condensates from cigarettes, cigars and pipe tobacco were mutagenic on Salmonella typhimurium TA100 and TA98 when activated with rat liver microsomal system. Mutagenicity of a unit weight of smoke condensate was rather high in cigars, low in pipe tobacco and intermediate in cigarettes. Specific mutagenic activity was almost comparable among smoke condensates from low- to high-tar cigarettes, although some variations were observed depending upon the country producing the cigarettes. Marked mutagenicity of cigarette smoke condensate could not be explained by the benzo (a) pyrene or nitroso compounds it actually contained, suggesting the presence of other very potent mutagens in tobacco smoke condensates.

Animals↗