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Biomedical subjects

T Suga

Publications and source records attributed to T Suga.

At least 325 records · Page 18Linked to original sources
PubMed1982

Changes in membrane surface properties of hepatic peroxisomes of rats under several conditions as determined by partition in aqueous polymer two-phase systems.

Changes in membrane surface properties of hepatic peroxisomes of rats under several conditions were observed by aqueous polymer two-phase systems, which contained 6% (w/w) dextran T 500, 6% (w/w) polyethyleneglycol 4000, 250 mmol sucrose/kg and various concentrations of sodium phosphate buffer. The partition of peroxisomes into the upper phase depended to a large extent on their membrane surface charge. The cross-points of peroxisomes shifted from 5.55 to 5.25 and 5.2 after the administration of clofibrate and aspirin for 2 weeks, respectively, although that of alloxan-diabetic rat peroxisomes was not altered. The hydrophobic properties of peroxisomes, examined by means of a partition containing polyethyleneglycol monostearate, were altered by diabetes and starvation, but no change occurred in rats treated with clofibrate or aspirin. In the liver of rats fed a high-fat diet, the partition of peroxisomes was the same as that of the control. These findings indicate that hypolipidemic drugs such as clofibrate and aspirin induce the proliferation of peroxisomes and lead to the alteration of the surface charge of peroxisomal membranes. Diabetes or fasting lead to an alteration mainly of the hydrophobic properties. Both changes are probably due to alteration of content and/or composition of the proteins and the phospholipids in peroxisomal membrane under the conditions used.

Animals↗
PubMed1982

A case of IgA nephropathy associated with marked hematuria after upper respiratory tract infections.

IgA nephropathy is characterized by recurrent hematuria which is occasionally associated with upper respiratory infections. Since serial follow-up of IgA nephropathy has not been reported, a 43 year old patient who showed a typical course of this disease is described. Hematuria was markedly increased each time after upper respiratory infections. Renal biopsy revealed typical features of IgA nephropathy by light and immunofluorescent microscopy. Marked hematuria subsided after administration of antibiotics. Upper respiratory tract infections might be a risk factor in some patients with IgA nephropathy. In some patients upper respiratory tract infections may exacerbate IgA nephropathy.

Adult↗
PubMed1982

Prevalence of IgAl deposits in Henoch-Schoenlein purpura (HSP) nephritis.

A study on the detection of IgA1, IgA2 and the J("joining") chain in glomeruli from renal biopsy specimens in patients with Henoch-Schoenlein purpura (HSP) nephritis is described. Renal biopsy specimens from five patients with HSP nephritis were stained with FITC-labelled anti-human IgA1, IgA2 and J chain, and then examined with a fluorescent microscope. IgA1 was detected in glomeruli from all patients with HSP nephritis examined in this study. IgA2 was not observed in any patients. These findings were similar to those for IgA nephropathy reported previously. Histopathological findings of glomeruli in HSP nephritis were almost identical to those in IgA nephropathy. The levels of serum IgA in patients with HSP nephritis increased as observed in IgA nephropathy. It is suggested that there are some immunopathological similarities between HSP nephritis and IgA nephropathy.

Adult↗
PubMed1982

Esophageal ulceration in a patient with Behcet's syndrome.

The gastrointestinal manifestation of Behçet's syndrome including colitis and esophageal ulceration has been reported in the past few years. However, esophageal ulceration associated with Behçet's syndrome is a rare condition. There appears to be only seven reports of esophageal ulceration associated with Behçet's syndrome. We have recently observed esophageal ulceration in a 64-year-old male known to be on case of untreated Behçet's syndrome (three major symptoms), who presented with epigastralgia and transient substernal discomfort just after swallowing food. In this patient, endoscopic examination revealed multiple esophageal ulceration and kissing-type gastric ulceration. The esophageal ulcers were not improved by frequent antacid therapy for six weeks, while gastric ulcers responded well to the therapy. Steroid therapy was begun and esophagoscopy revealed scarred ulcers after only two weeks. Therefore, steroid hormone therapy was thought to be of benefit in this patient. Esophageal ulceration associated with Behçet's syndrome, a rare condition, is reported and discussed in accordance with some reviews previously reported.

Behcet Syndrome↗
PubMed1982

[Changes of posterior osseous elements in narrowing of antero-posterior diameters of the lumbar spinal canal].

Narrowing of the antero-posterior diameters of the foramen and its influence to the posterior osseous elements were studied on 585 dry vertebral bones of 117 human lumber specimens. 1. Narrowing of the antero-posterior diameter of the foramen progresses even after reaching adult age and continues thereafter. Close correlations were found between the antero-posterior diameters and the transverse diameters and between the antero-posterior diameters and the area. 2. Narrowing of the antero-posterior diameters of the foramen was due to hypertrophy of the inside of the L3 to L5 lamina, but it is also due to shortening of the pedicle or compression of the posterior elements of L1 and L2 level. 3. Together with narrowing of the antero-posterior diameters of the foramen due to developmental stenosis and due to advanced age, the articular facets move on toward the sagittal plane keeping 90 degree angle between the laminae, and medial edges of the superior articular facets approach to the mid-line and add the depth of the lateral recesses, but the antero-posterior diameters of the lateral recesses do not correlate with narrowing of the antero-posterior diameters of the foramen. 4. Changes of the posterior osseous elements related to the narrowing of the antero-posterior diameters of the foramen are significant at the lower lumbar vertebrae of the male. It is therefore concluded that developmental inclination, muscular activity and advanced age were factors influencing the spinal canal stenosis.

Adult↗
PubMed1981

Biotransformation of aloenin, a bitter glucoside constituent of Aloe arborescens, by rats.

Aloenin has been established to be 4-methoxy-6-(2-beta-D-glucopyranosyloxy-4-hydroxy-6-methylphenyl)-2-pyrone; it shows an inhibitory activity for gastric juice secretion. Rats metabolized it to 4-methoxy-6-(2,4-dihydroxy-6-methylphenyl)-2-pyrone, 2,5-dimethyl-7-hydroxychromone and glucose, which were excreted in the feces and the urine. The distribution of the radioactivity originating from 14C-labeled aloenin was studied. The tracer found in the kidney and the liver reached 60% of the amount administered 24 h after feeding and decreased rapidly in the next 24 h.

Animals↗
PubMed1981

Effects of Triton WR-1339 on lipoprotein lipolytic activity and lipid content of rat liver lysosomes.

The characteristics of lipoprotein lipolytic activity in lysosomes after the administration of Triton WR-1339 were studied, and the observed decrease in the density of the particles is discussed. The light mitochondrial fraction prepared from rat liver according to the method of De Duve et al. (Biochem. J. (1955) 60, 604) was used as a crude lysosomal fraction, in which acid phosphatase and lipoprotein lipase activities were concentrated. The lipoprotein lipolytic activity of lysosomes had a pH optimum of 4.0. The activity was strongly inhibited by Triton WR-1339 in vitro at low concentrations, while the acid lipase activity was almost unaffected, though relatively high concentration of the detergent significantly inhibited the latter activity. When Triton WR-1339 administered to rats (150 mg/100 g body weight), the activities of both the lipoprotein lipase and acid lipase of lysosomes from the treated rats decreased to one-third of those of control rats. Low-density and high-density lysosomes were partially purified from the light mitochondrial fraction from Triton WR-1339-treated and silver colloid-treated rats, respectively, by sucrose density gradient centrifugation. The low-density lysosomes (d = 1.00-1.13) from Triton WR-1339 administered rats had approximately 4 and 3 times higher contents of triglyceride and cholesterol, respectively, than the high-density lysosomes (d greater than 1.30) from silver colloid-treated rats. In view of these results and the fact that the density of Triton WR-1339 is quite high (at least d = 1.20), the decrease in density of hepatic lysosomes upon Triton WR-1339 administration cannot be due simply to incorporation of the detergent, and may rather be a result of incorporation and accumulation of some lipid(s) (possibly as lipoprotein) into lysosomes together with Triton WR-1339.

Animals↗
PubMed1981

Changes in peroxisomal fatty acid oxidation in the diabetic rat liver.

Changes in fatty acid oxidation of peroxisomes in the liver of alloxan-diabetic rats were studied. After injection of alloxan (150 mg/kg, subcutaneously), the activity of peroxisomal cyanide-insensitive beta-oxidation increased more rapidly than that of carnitine palmitoyltransferase, which was the rate-limiting step of mitochondrial beta-oxidation, and reached 3 times the control level at 7 days after the treatment. The peroxisomal beta-oxidation activity was more potent toward medium chain acyl-CoAs (C=10 and 12), though it was extremely low for shorter chain lengths. The activity of carnitine acetyltransferase increased to 2.4 times the control level and the change appeared mainly in the peroxisomal fraction. On the other hand, the activity of palmitoyltransferase increased to twice the control level, distributed mostly in the mitochondrial fraction. The activity of carnitine acyltransferase increased mainly in the peroxisomal fraction, and was higher for shorter and medium chain acyl-CoAs. These results suggest that peroxisomal fatty acid oxidation and transport of acetyl-CoA and medium chain acyl-CoA as well as NADH product in peroxisomes may be rapidly enhanced in response to the demand of organs for the urgent supply of energy from fatty acids in the diabetic condition.

Animals↗
PubMed1981

Effects of some hypolipidemic drugs on biochemical values and on hepatic peroxisomal enzymes of normolipemic rat.

Effects of vitamin B2-butyrate, nicomol, ML-236B, KF 1492 and pantethine, which are hypolipidemic drugs, on biochemical values and on hepatic peroxisomal enzymes of normolipemic rat. 1) Vitamin B2-butyrate (100 mg/kg) and nicomol (lg/Kg) increased carnitine acetyltransferase and D-amino acid oxidase activities, respectively, while these drugs had no influence on body weight, liver weight, serum and liver triglyceride, and serum and liver cholesterol levels. 2) ML-236B (300 mg/kg) had no influence on biochemical values and on activities of peroxisomal enzymes containing catalase. 3) KF 1492 (300 mg/kg) had no influence on the biochemical values, but an increase in the activities of fatty acyl-CoA oxidizing system (FAOS) and carnitine acetyltransferase (CAT) participating hepatic lipid metabolism was observed. 4) Pantethine (lg/kg) had no influence on the biochemical values, except a little decrease in the growth rate. However, increase by about 10% in the activities of urate oxidase and D-amino acid oxidase was observed. Catalase activity was decreased to 60% of control level. From these results, it is concluded that, in contrast to clofibrate, vitamin B2-butyrate, nicomol, ML-236B, KF 1492 and pantethine have little influence on the lipid metabolism of normolipemic animal and on the hepatic peroxisomal enzymes, indicating that the action mechanism of these drugs may be different from that of clofibrate and that the participation of hepatic peroxisomes in hypolipidemic activities of these drugs may be little if any.

Animals↗
PubMed1981

Effects of butylated hydroxytoluene (BHT) on the level of glutathione and the activity of glutathione-S-transferase in rat liver.

Oral administration of 500 mg/kg of butylated hydroxytoluene (BHT) daily for three days elevated the total glutathione (GSH) level and the activities of GSH-S-transferase for CDNB and DCNB and GSSG reductase in the rat liver. BHT-alcohol, which is a metabolite of BHT, also elevated the level or activity of these components. The induction of these components with BHT-alcohol was slightly less than that with BHT. However, BHT or BHT-alcohol in doses used had no effect on GSH peroxidase activity.

Animals↗