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Biomedical subjects

T Suga

Publications and source records attributed to T Suga.

At least 289 records · Page 16Linked to original sources
PubMed1984

Effect of danazol on solubilization of immune deposits in patients with IgA nephropathy.

A study on the solubilization of glomerular immune deposits by administration of danazol in patients with IgA nephropathy is described. A clinical trial on danazol was performed in seven patient with IgA nephropathy. Administration of danazol was effective in improving proteinuria in patients with IgA nephropathy. In vitro effects of patients' sera on solubilization of glomerular immune deposits were examined in parallel studies. Renal biopsy specimens obtained from IgA nephropathy were incubated with fresh patients' sera before and after administration of danazol at 37 degrees C for one hour in plastic test tubes. The sections were stained with FITC-labeled heavy chain-specific anti-human IgA antiserum and then examined with a fluorescent microscope. It was shown that the solubilization of glomerular immune deposits by sera after administration of danazol from patients with IgA nephropathy was significantly higher than that by sera before such treatment.

Clinical Trials as Topic↗
PubMed1984

IgA nephropathy associated with HLA-DR4 antigen.

This report describes 2 siblings with IgA nephropathy. Patient No. 1 was a 38-year-old woman with hematuria and proteinuria of 19 years duration. Her blood ABO type was A and Rh positive. She was found to have HLA-A2,Aw24; Bw54 , Bw48 ;Cwl,C-;DR1,DR4. Her renal specimen was diagnosed as the advanced stage of IgA nephropathy histologically. Patient No. 2 was a 41-year-old man who was a brother of patient No. 1. His blood ABO type was O and Rh positive. His serotype for the HLA was found to be HLA-Aw24,A-;Bw35, Bw54 ;Cw1,Cw3;DR4, DRw9 . His renal histology showed the advanced stage of IgA nephropathy. It is suggested that an abnormal immune response linked to gene coding for HLA-DR4 antigen might be involved in the development of IgA nephropathy.

Adult↗
PubMed1984

Detection of IgA1-dominant immune complexes in peripheral blood polymorphonuclear leukocytes by double immunofluorescence in patients with IgA nephropathy.

The amounts of IgA1- and/or IgA2-dominant immune complexes included in peripheral blood polymorphonuclear cells (PMN) were determined by the double immunofluorescence technique in patients with IgA nephropathy. The aim of the present study was to determine the prevalence of IgA1-and/or IgA2-dominant immune complexes phagocytized by PMN in patients with IgA nephropathy. 5 patients with IgA nephropathy and 10 healthy adults were examined. It was demonstrated that the percentages of IgA1 with C3 cytoplasmic inclusion bodies were significantly increased compared with those of IgA2 with C3 cytoplasmic inclusion bodies in patients with IgA nephropathy. It was suggested that IgA1-dominant immune complexes are phagocytized by peripheral blood PMN in patients with IgA nephropathy.

Adult↗
PubMed1984

Solubilization of intraglomerular deposits of serum proteins by fresh human sera or gamma-globulin in patients with diabetic nephropathy.

The solubilization of renal deposits of IgG and other serum proteins by fresh human sera or human gamma-globulin was studied in patients with diabetic nephropathy. Renal biopsy specimens were from 10 patients with diabetic nephropathy. These specimens were incubated with fresh sera obtained from the same patients and healthy adults or human gamma-globulin at 37 degrees C for 1 hr in plastic test tubes. The sections were stained with FITC-labeled heavy chain specific anti-human IgG, alpha 1-antitrypsin, haptoglobin and beta-lipoprotein antisera, and then examined with a fluorescent microscope. It was demonstrated that fresh human sera or gamma-globulin significantly solubilized glomerular deposits of IgG and other serum proteins in patients with diabetic nephropathy. It was postulated that solubilization of protein deposits in glomeruli requires the same substances detected in vitro in the kidney tissues from patients with diabetic nephropathy.

Adult↗
PubMed1984

Solubilization of intraglomerular deposits of IgG immune complexes by human sera or gamma-globulin in patients with lupus nephritis.

A study of the solubilization of glomerular deposition of IgG immune complexes by sera from patients with lupus nephritis is described. Renal biopsy specimens were obtained from 11 patients with lupus nephritis, five patients with IgA nephropathy and one patient with minimal change nephrotic syndrome. These renal specimens were incubated with fresh, stored or heated sera from the same patients or healthy adults and human gamma-globulins at 37 degrees C for 1 h in plastic test tubes. The sections were stained with FITC conjugated heavy chain specific anti-human IgG or C3 antisera and then examined with a fluorescent microscope. The sections were also stained with FITC conjugated human gamma-globulins and rhodamine conjugated anti-human IgG, IgM or IgA antisera and then examined by double exposure under a fluorescent microscope. It was demonstrated that fresh human sera or gamma-globulins significantly solubilize glomerular immune deposits in patients with lupus nephritis in vitro. It was indicated that the solubilization of IgG glomerular deposits from patients with lupus nephritis does not depend on complement. It is postulated that solubilization of immune deposits in glomeruli requires the excess amounts of antigenic substances in patients with lupus nephritis.

Antigen-Antibody Complex↗
PubMed1984

Cold reacting anti-nuclear factor (ANF) in families of patients with IgA nephropathy.

The emergence of cold reacting anti-nuclear factor (ANF) in families of patients with IgA nephropathy was examined to determine whether some immunological alterations among family members affect the development of this disease. The procedure for the detection of the cold reacting ANF was reported previously. Fifty-five per cent of sera from 66 relatives of IgA nephropathy 24 patients was found to have the IgM cold reacting antibody. The incidence of ANF in healthy adults was 3%. Both household and non-household consanguineous relatives showed antibody in their sera. Sixty-five per cent of consanguineous relatives who had close household contacts with IgA nephropathy patients showed cold reacting ANF, whereas only 10% of non-consanguineous relatives who had close household contact had this antibody. It is suggested that familial susceptibility or genetic factors, in addition to environmental factors, may be responsible in the development of IgA nephropathy.

Adolescent↗
PubMed1984

Two cases of acute poststreptococcal glomerulonephritis superimposed on rheumatoid arthritis.

Recently, the rheumatoid factor (RF) has been postulated to play a role in the development of acute poststreptococcal glomerulonephritis (APSGN). However, there are few reports in which APSGN is superimposed on rheumatoid arthritis (RA). Two patients are reported in this paper who showed atypical renal histopathological findings as APSGN. It was suggested that renal histopathological finding might become different from those of typical APSGN when it is superimposed on RA.

Adult↗
PubMed1984

In vivo alteration of antibody production in patients with IgA nephropathy.

Augmentation of IgA production has been postulated for the development of IgA nephropathy. An influenza HA vaccine was administered to healthy adults and patients with IgA nephropathy to elucidate if there was any in vivo alteration of antibody production in response to antigenic stimulation in these patients. The vaccine was administered s.c. in a dose of 350 CCA units at an interval of 4 weeks. IgG, IgA and IgM class antibodies to influenza HA antigens and three classes of rheumatoid factors (RF) were determined using a solid phase fluorescence immunoassay. The titres of IgG class antibodies to influenza HA antigens did not change significantly in either group after the vaccination. No significant differences were observed in the titres of IgG antibodies between the two groups. IgA antibodies were significantly increased only in patients in the 4th week and continued to the 8th week. The titres of IgA antibodies were always higher in patients than in controls during the study period. IgM antibodies were significantly increased stepwise in both groups to an equal degree. IgG and IgA RF were always higher in patients than in controls. IgM RF were significantly increased and higher than in controls in the 8th week in patients. It is concluded that patients with IgA nephropathy might be high responders for IgA antibody production, and that polyclonal activation might be associated with increased IgA production following in vivo antigenic stimulation in these patients.

Adult↗
PubMed1984

Aberration of B and T cell subsets in patients with IgA nephropathy and membranous nephropathy.

Peripheral blood lymphocytes from 15 patients with IgA nephropathy (IgAN), 10 patients with membranous nephropathy (MN) and 15 healthy adults were examined to investigate whether there are any aberrations in B and T cell subsets in these diseases. F (ab')2 portions of heavy chain specific anti human IgG, IgA and IgM antibodies were used to determine isotypes of B lymphocytes, and monoclonal antibodies directed at human T cells termed OKT3, OKT4 and OKT8 antibodies were used to assay subsets of T cells. Quantitation of B and T cell subsets was performed by flow cytometry. IgG, IgA and IgM bearing peripheral blood lymphocytes were significantly increased in patients with both IgAN and MN, although the dominant class of immunoglobulin bearing cells in patients with IgAN was IgA bearing cells and that in patients with MN was IgM bearing cells. OKT4 positive (OKT4+) and OKT8 positive (OKT8+) cells were significantly decreased in both patient groups. The OKT4+/OKT8+ ratio increased only in MN patients without steroid therapy. It was suggested that aberrations of B and T cell subsets are common in both diseases.

Adolescent↗
PubMed1984

A case of mesangial IgM nephropathy with decreased renal function.

A case of mesangial IgM nephropathy associated with chronic renal failure is described. The patient did not reveal nephrotic syndrome during the clinical course. Renal biopsy specimens revealed typical features of mesangial IgM nephropathy when studied by light microscopy, electron microscopy and immunofluorescence staining. Mesangial IgM nephropathy is considered to be a heterogenous disorder, because this disorder is clinically characterized by nephrotic syndrome, mild proteinuria and/or hematuria or renal failure.

Autoimmune Diseases↗
PubMed1984

The noninvolvement of MDH as NAD-oxidoreductase shuttle in rat liver peroxisomes.

Subcellular localization of malate dehydrogenase and glycerol-3-phosphate dehydrogenase in rat liver was studied by sucrose density gradient centrifugation. The specific adsorption of cytosolic malate dehydrogenase to the peroxisomes was observed. This phenomenon was eliminated by washing peroxisome-rich fraction with 100 mM potassium chloride. It is suggested that the malate shuttle between the cytosol and the mitochondria is more dominant than the glycerophosphate shuttle with respect to the transfer of reducing equivalents, while NADH produced by fatty acid oxidation in peroxisomes can not be transferred into the cytosol via the malate shuttle in the rat liver.

Animals↗
PubMed1984

Antitumor activity of lentinan in murine syngeneic and autochthonous hosts and its suppressive effect on 3-methylcholanthrene-induced carcinogenesis.

The antitumor effect of lentinan in syngeneic and autochthonous tumor-host systems and its suppressive effect on 3-methylcholanthrene (MC)-induced carcinogenesis were confirmed using DBA/2 and SWM/Ms hosts. The regressive activity of lentinan against the solid form of Sarcoma 180 was the most effective in DBA/2, SWM/Ms, or A/J mice and less effective in C3H/He or C57BL/6 mice. The growth of a syngeneic MC-induced DBA/2.MC.CS-1 fibrosarcoma (native and trypsinized) was markedly inhibited, and the regression of tumors was detected by the i.p. injection of minute amounts of lentinan into DBA/2 mice, which were the most suitable host in lentinan treatment. When DBA/2 mice were used, lentinan was also effective for even autochthonous primary tumors induced within 15 weeks after MC inoculation, but less effective for tumors induced during the 16 to 36 weeks after MC treatment. Lentinan showed a prominent suppressive effect in MC-induced carcinogenesis using DBA/2 and SWM/Ms mice but not effect when BALB/c, C57BL/6, or C3H/He mice were used. The timing of lentinan administration in the latter result was examined using SWM/Ms mice, and lentinan, when it was given daily for 10 days after the third week of MC inoculation, was strikingly effective (33%), but not so effective (63%) when lentinan was given after the sixth week of MC treatment, compared with tumor-occurrence rate in the control group (88%). The reason why DBA/2, SWM/Ms, or A/J mice were suitable hosts for lentinan treatment is not clear, but the natural killer capability or phagocytic macrophage function in these strains seems to have no relation to lentinan action, because A/J mice are deficient in natural killer function, and in these strains of mice the phagocytic function of macrophages is weak. It may be quite possible that these strains of mice are most sensitive to delayed-type hypersensitivity and/or cytotoxic T-cell response in which T-cells and lentinan play important roles. The tumor-host systems presented here provide a good model in which lentinan retains an inhibitory capacity in syngeneic and autochthonous hosts, and such a model offers the possibility for further study of the host defense mechanism against cancer.

Animals↗
PubMed1984

Effects of a "single shot" of urokinase on fibrinolytic activities in patients with IgA nephropathy.

A study on the clinical effects of urokinase in patients with IgA nephropathy is described. Three different methods of administration, including single, continuous, and mixed administration, were employed in this study. Measurements of plasminogen, plasmin and alpha 2-plasmin inhibitor levels in plasma were performed during the course of urokinase administration in patients with IgA nephropathy and chronic proliferative glomerulonephritis. Measurements of alpha 1-anti-trypsin and alpha 2-macroglobulin levels were also performed in these patients. Urinalysis was performed both before and after administration of urokinase. It was demonstrated that a single shot of urokinase induced a significant fibrinolytic activity in patients with IgA nephropathy, and that a single shot of urokinase was effective in improving proteinuria and/or hematuria in patients with IgA nephropathy. It is concluded that a single shot of urokinase may be useful for treatment of patients with IgA nephropathy.

Fibrinolysis↗