Biomedical subjects
Publications and source records attributed to T Somogyi.
The authors present a case history for a two-year-old boy in whom the study of perianal ulcer helped to diagnose the Langerhans cell histiocytosis. The authors emphasize the diagnostic value of intertriginous ulcers in identifying the above-mentioned disease.
Authors observed a boy presented a protrusion positioned lumbosacral, existing since his birth. Later, on inner side of both heels ulcers began to take shape, being not curable by local treatment. Neurologic examination verified severe radicular lesion, magnetic resonance investigation showed lumbosacral lipo-meningocele. After its surgical treatment, ulcers on soles healed up. Neurotropic ulcer develops on sites exposed to pressure, being generally painless. Several sickness may lead to its development. In childhood occurrence is relatively rare. At this age mostly various closure troubles of medullary tube may stand in the background. To successful treatment of malum perforans--besides local therapy--that of underlying illness is essential as well.
A major protein of Mr 68,000 with a previously described protein kinase activity (PK68), which is induced in HCMV-infected cells, is shown to be virus encoded by means of hybrid selection. The gene coding for the corresponding 4-kb mRNA was located within map units 0.510 and 0.525. This location is the same as that of pp65, a structural protein with no attributed function. The early nature of the protein was confirmed with its mRNA first appearing 3 hr after infection under cycloheximide reversal and phosphonoacetic acid blocking conditions. Another mRNA, 97 nucleotides smaller and 5' coterminal with PK68, which codes for a Mr 52,000 protein, was also observed.
All DNAs from human cytomegalovirus studied (57 fresh isolates, 3 reference strains) showed hybridization under stringent conditions to a plasmid containing strain Ad-169 transforming sequences. Analysis of hybridization patterns obtained with DNAs digested by three different enzymes allowed construction of a global map for this highly conserved area of the genome.
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In this study, we have established the colinearity of human cytomegalovirus (HCMV) genomes using stringent conditions of DNA-DNA filter hybridization of HCMV HindIII fragments and cosmid-cloned AD169 strain HCMV DNA fragments. Large cosmid-cloned fragments of AD169 DNA were used for the preparation of radioactive probes by nick translation. These probes were hybridized to HindIII digests of DNA from three fresh isolates of HCMV and to that of the Davis strain. Using published HindIII restriction maps for the AD169 strain as a reference, the results obtained by hybridization allowed us to construct HindIII restriction maps for the genomes of the three fresh isolates. Confirmation of our methodology was found in the correspondence between the HindIII map we constructed for the Davis strain and that published previously. Furthermore, it is shown that variation in the restriction profiles of the unique regions of the genome are due to the absence or gain of restriction sites, and not to a rearrangement of fragments. This technique allows rapid construction of physical maps of the DNA of any fresh isolate for a given restriction enzyme provided the corresponding restriction map of a strain to be used as reference is available.
Specific histological alterations have been observed in 23 of the 25 patients with early syphilis with laboratory findings suggesting liver damage. Treponema has been detected in the areas of liver lesion in 70 per cent of the patients. Control examination carried out after penicillin therapy showed regression of the alterations. On the basis of the findings authors assert that treponema might induce hepatitis in early syphilis differing from liver damage of other origin.
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17 out of 175 cases of early syphilis had clinical, biochemical, and immunological evidence of liver damage. Before penicillin therapy the histological appearance of the liver was abnormal in 14 of the 15 patients from whom biopsy specimens were obtained. In 7 cases, treponemes were seen in the liver. After two months' penicillin therapy the extent and severity of the histological abnormality was reduced. In the repeat liver-biopsy specimens obtained after penicillin treatment no treponemes could be demonstrated. It is suggested that the hepatitis found in these 17 cases of early syphilis was produced by treponemes.
In 17 out of 176 cases of early syphilis (seropositive syphillis I; syphilis II) liver function tests yielded a positive result. In these patients a significant increase in the serum IgG, IgM and coeruloplasmin levels and a decrease in t4e transferrin level was found. The concentrations of alpha-2-macroglobulin and of beta-1-C-globulin were practically uneffected. Liver biopsy revealed hepatitis of variable severity in 13 patients with focal necroses or a proliferative process effecting the walls of the central veins, the arterioles and the branches of the portal vein. In 7 cases the presence of Treponema in the liver was demonstrated.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.