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Biomedical subjects

T Smith

Publications and source records attributed to T Smith.

At least 613 records · Page 34Linked to original sources

Effect of amount and composition of feed given over three lactations on the performance of the dairy cow.

Eighty-nine autumn-calving first calf and adult Friesian cows participated in an experiment on the effect of feeding over three lactations on milk production and live weight change. Fixed daily allowances of digestible energy (DE) formed two of the treatments (h, H; moderate, M). Diets of similar composition were used for both treatments and rations were weighed daily for each cow. The cows within these treatments were re-randomized to H or M at second and again at third parturition on experiment. A further treatment (ALF), applied continuously over three lactations, consisted of the M allowance of compound feed, weighed daily for each cow, plus as lib. weighed, group-fed forages. The ALF animals were randomized for each lactation into two groups both of which received the same total compound feed allowance over the first 26 weeks of lactation. For one group (Flat) equal amounts were given daily whilst for the other group (Step) the daily amount was decreased monthly. After week 26 equal rations were fed. Hay, maize silage and grass silage formed the forages in winter. Grass, cut for the H and M groups but grazed for the ALF group, provided the summer forage. Energy intakes covered some 80-110% of requirements. Yields of milk and of milk solids responded similarly for both parties. In the first experimental lactation, treatment H led to greater yields compared with M. H also led to smaller losses of live weight in early lactation, equal gains in mid lactation, and smaller gains in late lactation and the dry period, compared with M. Extension of H into a second lactation increased the advantage in milk and solids yields observed in the first lactation on experiment. Recovery of body reserves on treatment M continued. Treatment H in a second lactation on experiment after M in the first lactation led to even greater compensatory gains in live weight at the expense of milk production. There was no effect in the third lactation of experiment of treatments applied in the first lactation. Treatments H and M applied factorially over lactations 2 and 3 gave the same pattern of treatment effects as in lactations 1 and 2. Treatment ALF broadly supported the same milk yield and live weight change as treatment H but improved fat, protein and lactose yields. Within treatment ALF, Flat and Step distribution of compound led to equal performance. Multiple lactation effects of ALF equalled those of H. The effects on milk composition of H compared with M treatment were variable. In general an advantage accrued to ALF over M but without long term effects.

Animal Feed↗

Clarifying comments on the young autism study: reply to Schopler, Short, and Mesibov.

Lovaas (1987) has been criticized for overstating the outcome of an intensive behavioral intervention for young autistic children. Part of the criticism reflects skepticism of Lovaas's view that it is possible to overcome autistic children's deficits. However, Lovaas's study built on a substantial body of research that has suggested just such a possibility. It also contained a number of methodological features that provide evidence supporting his interpretation of the outcome, notably, unbiased control groups and multiple pretreatment and follow-up measures.

Autistic Disorder↗

Nutrient supply and growth of cattle offered straw-based diets.

An experiment was conducted using steers cannulated at the rumen, duodenum and ileum to study the effects of increasing the levels of barley and fishmeal in straw-based diets. Diets A, B, C and D contained ammonia-treated straw, barley and fishmeal in the ratios, 67:33:0, 66:23:11, 53:47:0 and 52:36:12 (by weight) and were offered in daily amounts of 3.9, 3.9, 4.8 and 4.8 kg dry matter. The effects of barley were attributable to increased intakes of digestible organic matter and consequently to increased flows of microbial matter to the duodenum. There were no modifications in the balance of energy to nitrogen-yielding nutrients available for absorption. Introducing fishmeal into diets improved digestibility of cellulose and xylose by up to 6.7 and 4.7% respectively, and shifted digestion towards the large intestine. Second, it increased amino acid N supply to the small intestine which averaged 52.2, 63.2, 68.8 and 84.0 g/d with diets A, B, C and D. Some changes were also noted in the balance of amino acids absorbed. Consequently, the contribution of amino acids to metabolizable energy intake increased with the proportion of fishmeal in diets (0.17, 0.20, 0.18 and 0.21 for diets A, B, C and D). Growth rates measured in heifers amounted to 259, 431, 522 and 615 g/d for diets A, B, C and D. They appeared to be related to intestinal amino acid supply.

Amino Acids↗

Molecular characterization of HIV-1 isolated from a serum collected in 1976: nucleotide sequence comparison to recent isolates and generation of hybrid HIV.

Human immunodeficiency virus type 1 (Z321 designate, HIV-1Z321), the oldest known HIV, was isolated from a serum sample collected in Zaire in 1976 and was molecularly cloned. Restriction enzyme analysis of unintegrated viral DNA revealed the presence of conserved restriction enzyme cleavage sites in the long terminal repeat sequences. Nucleotide sequence analysis of the 3' end of the viral DNA revealed a pattern similar to other HIV-1 isolates described. However, some of the common restriction sites present in other isolates were absent in HIV-1Z321. The extent of differences between HIV-1Z321 and recent isolates from North America and Zaire was 17.86-18.36% on the nucleotide sequence level and 26.5-33.2% difference in the predicted amino acid sequence in the envelope gene. Differences were also noted in 3'-orf (nef: according to HIV gene nomenclature; see Ref. 42) gene and U3 region of the long terminal repeat sequences of HIV-1Z321 and other isolates. Nucleotide sequence of a HIV-1 isolate, 12 years apart from the present isolates, will provide an important time calibration point for the evolutionary divergence of HIV isolates. Hybrid HIV was also generated by transfecting HIV-1Z321 and HIV-1HTLV-III viral DNAs into cells.

Amino Acid Sequence↗

Trial of intravenous lidocaine on painful neuropathy in cancer patients.

In 10 cancer patients with cutaneous allodynia, intravenous lidocaine (5 mg/kg body weight) or 0.9% NaCl was given in a double blind, cross-over study to determine the analgesic effect. One patient had complete and one had partial pain relief with lidocaine infusion, whereas three patients experienced partial pain relief with placebo. Neither lidocaine nor placebo reduced pain intensity or consumption of analgesics significantly during the study period. Intravenous infusion of lidocaine cannot be recommended as routine pain treatment in cancer patients with cutaneous allodynia or pain, but further studies are needed to test the effect of lidocaine on different peripheral stimuli.

Adult↗

Preliminary study of the vascular dynamics of port-wine hemangioma with therapeutic implications for argon laser treatment.

The vascular dynamics of port-wine hemangioma have been studied in several ways in order to better understand blood flow factors. Utilizing a laser Doppler velocimeter, differential perfusion/blood flow was studied and contrasted to normal skin, compared to heat and cold challenges, and finally measured in relationship to argon laser treatment. Results indicate that port-wine hemangiomas do not necessarily have different perfusion than normal skin but respond less vigorously to heat challenges. Cooling showed no uniform response by port-wine hemangioma vessels, while injection with Xylocaine plus epinephrine resulted in a markedly decreased perfusion and vasoconstriction contrary to previously held theories. Argon laser treatment did not uniformly alter laser Doppler perfusion to a predictable degree. Laser Doppler velocimeter flow studies were not able to predict future good versus bad results of laser treatment.

Adolescent↗

Somatosensory evoked potentials in the evaluation of patients with stocking/glove paresthesias.

We studied 10 patients referred for suspicion of peripheral neuropathy. They all complained of paresthesias with a stocking distribution. As EMG, motor and sensory nerve conduction studies failed to confirm the clinical diagnosis, we studied somatosensory evoked potentials (SEP) following median and tibial nerve stimulation. The SEP findings were compared with controls and 10 spastic paraplegias. The evoked potential study revealed prolonged latencies of cortical potentials after tibial nerve stimulation in all the patients with paresthesias and were considered evidence of myelopathy.

Adult↗

A medical algorithm for detecting physical disease in psychiatric patients.

An algorithm for screening psychiatric patients for physical disease was empirically derived from a comprehensive assessment of 509 patients in California's mental health system. The first 343 patients were used to develop the algorithm, and the remaining 166 were used as a test group. Calculations were made for several versions of the algorithm, and the data were compared with the diagnoses listed in the patients' admission mental health record. The algorithmic procedure was more accurate and more cost-effective than the medical evaluation procedures used by the state mental health system. When applied to the test group, the algorithm detected up to 90 percent of patients who had an active, important physical disease at a cost of $156 per patient. The mental health system had detected 58 percent of test-group patients with a disease at a cost of $230 per patient.

Algorithms↗

Decentralized hospital management: rationale, potential, and two case examples.

Sunnybrook Medical Centre (SMC) in Toronto designed a unique organizational model for managing hospital services and programs in three clinical units. The SMC model, like a similar model at The Johns Hopkins Hospital (JHH), emphasizes the active participation of physicians in management, the decentralization of budgetary control, and accountability for resource use to the clinical unit level. Expanding on the JHH model, SMC implemented additional financial incentives to encourage changes in clinical practice which would bring direct financial benefit to the respective clinical unit, and thereby to the medical center. To improve quality of service, SMC has also expanded the JHH model of multidisciplinary and participatory approaches to patient care by standardizing managerial roles and responsibilities of each member of the management team. A brief description of issues related to implementation of these unique models summarizes the program's success.

Baltimore↗

Comparison of the effects of cetamolol and atenolol on epinephrine- and isoproterenol-induced hypokalemia in anesthetized dogs.

Epinephrine-induced hypokalemia is a beta 2-adrenoceptor-mediated effect known to occur in patients after acute myocardial infarction. Cetamolol and atenolol are beta-adrenoceptor antagonists that possess cardioselectivity. They were studied for their ability to inhibit epinephrine- and isoproterenol-induced hypokalemia in anesthetized dogs at equipotent beta 1-adrenoceptor blocking doses. Cetamolol was able to block epinephrine-induced hypokalemia completely, in a dose-related manner, and to block isoproterenol-induced hypokalemia partially. On the other hand, atenolol could produce only partial blockade of epinephrine-induced hypokalemia in a dose-related manner and had essentially no effect on isoproterenol-induced hypokalemia. These results showed that cetamolol was less cardioselective than atenolol and suggest cetamolol would be more beneficial than atenolol in preventing epinephrine-induced hypokalemia in patients who have an acute myocardial infarction.

Acetamides↗

Choroidal neovascularization in an eye with a macular hole.

We report the case of a 68-year-old woman who initially presented with an idiopathic macular hole OD and atrophic macular degenerative changes and who subsequently developed subretinal neovascularization OU. The subretinal neovascularization evolved through the macular hole OD and decreased visual acuity to counts fingers, but the membrane was successfully treated OS with krypton laser photocoagulation and stabilization of visual acuity at 20/50 over the eight-year postoperative follow-up. This is the first report, to our knowledge, of a membrane developing through a macular hole. This may be a more common occurrence than reported due to obscuring of details from hemorrhage and exudate as part of the disciform process.

Aged↗

Studies of human leprosy lesions in situ using suction-induced blisters. 1. Cellular components of new, uncomplicated lesions.

The cellular contents of blisters induced by suction over new, uncomplicated leprosy lesions, and in the skin of cured, control patients, have been examined with enzyme- and immuno-histochemical staining over a period of 4 days. The total cellularity of the blisters varied over a wide range, not correlated with the type of leprosy. Mononuclear cells predominated at all times studied, with nearly equal percentages of monocytes and T lymphocytes. The T-helper: suppressor ratio was significantly greater in BT than in BL and LL lesions at 48 hr. Suction blisters offer a painless, quantitative, reproducible, multiple-sampling method for obtaining cells from the cutaneous infiltrates of leprosy for phenotyping or functional analysis.

Blister↗

Diversity of antigens expressed on the surface of erythrocytes infected with mature Plasmodium falciparum parasites in Papua New Guinea.

Antigens were detected on the surface of erythrocytes from children with acute falciparum malaria in Madang, Papua New Guinea. These parasite-induced erythrocyte surface antigens (PIESA) were serotyped with convalescent sera from children and hyperimmune sera from adults in parasite infected cell agglutination assays (PICAs) and by inhibition of binding of infected cells to melanoma cells. Extensive serological diversity of PIESA was demonstrated. A significant correlation between serotypes defined by reactivity of immune sera in PICA and inhibition of melanoma cell binding (MCB) was observed. This suggests that both assays measure antibody responses to the same antigen(s). Increased recognition of different PIESA specificities with age is consistent with the hypothesis that repeated exposure to malaria confers immunity against a range of PIESA serotypes and parallels the development of clinical immunity to malaria in this area of Papua New Guinea.

Adult↗

Gastric pseudolymphoma and its relationship to malignant gastric lymphoma.

Gastric pseudolymphoma is generally considered a benign, reactive condition to chronic inflammation. We report a patient who, although initially diagnosed as having a gastric pseudolymphoma, was found to have a malignant gastric lymphoma 5 yr later. A review of the literature revealed 12 previously reported cases in which gastric pseudolymphoma was associated with a malignant gastric lymphoma. The premalignant nature of gastric pseudolymphoma is discussed, and frequent endoscopic surveillance to exclude transition to malignant lymphoma is recommended.

Aged↗

Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La.

To learn more about the mechanism and regulation of the ATP-dependent protease La in Escherichia coli, the lon gene was completely sequenced using the dideoxy method on fragments generated by Bal31 digestion. The predicted amino acid composition based on the DNA sequence agreed well with the composition of the acid-hydrolyzed protease. The predicted NH2-terminal amino acid sequence, tryptophan content, and the carboxyl terminus also agreed with experimental data. However, the molecular weight of 87,000 (783 amino acids) calculated from the DNA sequence was lower than prior estimates. The tetrameric enzyme contains four binding sites for ATP, a DNA-binding domain, a proteolytic site, and a regulatory site that binds unfolded polypeptides. An ATP-binding pocket exists on each subunit as shown by consensus sequences and elements of secondary structure resembling those on other nucleotide-binding proteins (e.g. adenylate kinase, RecA). For this purpose, improved consensus patterns for identifying ATP-binding domains were developed. Computer-assisted comparisons, however, failed to demonstrate any regions homologous to sequences in other polypeptides including proteases or DNA-binding proteins. This enzyme also contains an unusual highly acidic domain surrounded by very basic sequences. Protease La is the first ATP-dependent protease sequenced and seems to represent a new type of enzyme. The promoter sequence was similar to consensus sequences for other heat-shock promoters. Using site-directed mutagenesis, alterations were introduced into the putative promoter sequence. Mutations upstream of -35 had little effect, but alterations immediately upstream of -10 lowered basal transcription of a lon-lacZ operon fusion and reduced its response to inducers of the heat-shock response.

ATP-Dependent Proteases↗