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T Shiono

Publications and source records attributed to T Shiono.

At least 19 recordsLinked to original sources
PubMed1999

Isolation of a germin-like protein with manganese superoxide dismutase activity from cells of a moss, Barbula unguiculata.

A novel extracellular Mn-superoxide dismutase (SOD) was isolated from a moss, Barbula unguiculata. The SOD was a glycoprotein; the apparent molecular mass of its native form was 120 kDa, as estimated by gel filtration chromatography, and that of its monomer was 22,072 Da, as estimated by time of flight mass spectroscopy. The protein had manganese with a stoichiometry of 0.80 Mn/monomer. The cDNA clone for a gene encoding the extracellular Mn-SOD was isolated. Sequence analysis showed that it has a strong similarity to germin (oxalate oxidase) and germin-like proteins (GLPs) of several plant species and possesses all the characteristic features of members of the germin family. The clone encoding this extracellular Mn-SOD was therefore designated B. unguiculata GLP (BuGLP). BuGLP had no oxalate oxidase activity. In addition, the cDNA for a gene encoding the moss mitochondrial Mn-SOD was isolated. Its amino acid sequence had little similarity to that of BuGLP, even though a close similarity was observed among the mitochondrial Mn-SODs of various organisms. BuGLP was the first germin-like protein that was really demonstrated to be a metalloprotein with Mn-SOD activity but no oxalate oxidase activity.

Amino Acid Sequence↗
PubMed1999

Transradial coronary intervention in Japanese patients.

The objectives of this study was to test the feasibility and safety of transradial coronary intervention (TRI) in Japanese patients. We compared the results of TRI in 1,791 lesions (1,360 patients) between November 1995 and December 1997 with those of transfemoral coronary intervention (TFI) in 966 lesions (793 patients) between April and October 1995. We also examined the radial artery pulse in 294 patients 1-2 weeks after TRI by palpation and Doppler examination. Arterial puncture, coronary artery cannulation, lesion, and patient success rates were similar in both groups. No significant difference was noted in the major complication rate. Local complications were significantly lower in the TRI group (0.3% vs. 3.3%, P < 0.001). Doppler studies of the radial artery were performed in 294 patients chosen at random. In the first 234 patients, the sheath was pulled out 3 hr after the procedure, and in the last 60 patients, immediately after the procedure; radial artery occlusion rates were 5% and 0%, respectively. In conclusion, TRI seems safe and feasible in Japanese patients.

Aged↗
PubMed1999

[Atresia of the right atrial orifice of the coronary sinus with persistent left superior vena cava: a case report].

A 39-year-old woman presented with atresia of the right atrial orifice of the coronary sinus with a persistent left superior vena cava detected at cardiac catheterization. She was admitted with frequent episodes of angina at rest and on exertion. Coronary angiography, including spasm provocation test, yielded normal results. However, left coronary arteriography demonstrated a dilated coronary sinus and a persistent left superior vena cava draining into the innominate vein. The contrast medium leaked slightly into the right atrial cavity through the obstructed orifice of the coronary sinus. Atresia of the coronary sinus orifice is a rare malformation usually found at autopsy. Only 3 cases have been reported in Japan. This is the first adult Japanese case detected when the patient was still alive.

Adult↗
PubMed1997

Analysis of Nitrogen Adsorption Isotherms for a Series of Porous Silicas with Uniform and Cylindrical Pores: A New Method of Calculating Pore Size Distribution of Pore Radius 1-2 nm

Two kinds of the porous silicas with uniform and cylindrical pores were prepared from calcined Na4SiO4 and cationic surfactants ([C16H33N(CH3)3]Cl and [C18H37N(CH3)3]Br). In this paper, we call these porous silicas PS-C16 and PS-C18. PS-C16 was calcined in vacuo at 873-1273 K in order to prepare a series of porous silicas having different pore sizes. All nitrogen adsorption isotherms for a series of the porous silicas show no hysteresis in the adsorption and desorption branches. The nitrogen adsorption isotherms were analyzed by the t method, and the pore surface area (Sp), the pore volume (Vp), and the core volume (Vc) were determined. The pore radius (rp), the core radius (rc), and the adsorbed thickness on the pore wall (tpore) were computed from the measured values of Sp, Vp, and Vc. We can determine the relationship between the Polanyi adsorption potential and the core radius in the range of rc = 0.6-1.2 nm. On the basis of the present data, we propose a new method of calculating the pore size distribution in the range of rp = 1-2 nm.

Journal Article↗
PubMed1997

[An experience with omentopexy for the repair of postoperative bronchopleural fistula].

Postoperative bronchopleural fistula has been the most troublesome complications in the thoracic surgery. In this report, we presented a case of bronchopleural fistula successfully closed by omentopexy. A 51-year-old man had undergone left upper lobectomy and S6 segmentectomy for primary lung cancer. Bronchopleural fistula due to postoperative pneumonia was developed and completion pneumonectomy with the intercostal-musclo-pexy was performed. Post-re-operative course was unsuccessful, bronchopleural fistula remained, so we tried re-closure of the bronchial stump by omentopexy without thoracoplasty or muscle flap plombage. About a half year after 3rd operation, he relapsed into bronchopleural fistula. Then fibrin gluing was performed via a flexible fiberoptic bronchoscope without hospitalization, and the omental flap was fixed completely to the bronchial stump. We believe the omentopexy a useful procedure for treating postoperative bronchopleural fistula which can't make any chest-wall deformation.

Bronchial Fistula↗
PubMed1996

Functional magnetic resonance imaging (fMRI) during mental writing with phonograms.

Functional magnetic resonance imaging (fMRI) at 1.5 T using a gradient echo echo-planar sequence was employed to identify brain regions activated during the performance of a mental writing task using phonograms. Four regions were activated in all six subjects; the region surrounding the left intraparietal sulcus, the region surrounding the middle part of the left precentral sulcus and the posterior part of the left superior frontal sulcus, the region surrounding the right intraparietal sulcus, and the region surrounding either or both of the left and right cingulate sulci. The left intraparietal region was usually the most extensively activated. The results suggest that these four regions particularly the left intraparietal region, are essential in writing with phonograms. Since the left hemisphere was more extensively activated than the right, fMRI during the mental writing task is a good candidate for determining non-invasively which hemisphere is dominant for language.

Adult↗
PubMed1996

Autosomal dominant cone-rod dystrophy associated with mutations in codon 244 (Asn244His) and codon 184 (Tyr184Ser) of the peripherin/RDS gene.

OBJECTIVE: To characterize clinical findings associated with mutations in codon 244 (Asn244His) and codon 184 (Tyr184Ser) of the peripherin/RDS gene. DESIGN: Case reports with clinical features and results of fluorescein angiography, electroretinography, kinetic visual field testing, and DNA analysis. SETTING: University medical center. PATIENTS: Four affected members of two Japanese families with autosomal dominant cone-rod dystrophy associated with transversion mutations in codon 244 (Asn244His) and codon (Tyr184Ser) of the peripherin/RDS gene. RESULTS: Characteristic features included the initial symptoms of decreased visual acuity, macular degeneration, central or paracentral scotoma, cone-mediated electroretinographic responses that were more impaired than rod-mediated responses, and pigmentary degeneration in the midperipheral retina in the late stage. These phenotypic features corresponded to cone-rod dystrophy type 2a by the classification of Szlyk and associates. CONCLUSIONS: The Asn244His and Tyr184Ser mutations in the peripherin/RDS gene cause con-rod dystrophy type 2a. These findings imply that a mutation in codon 244 or codon 184 of the peripherin/RDS gene affects the functions and/or structural stability of cones and rods.

Adult↗
PubMed1996

Congenital hypertrophy of the retinal pigment epithelium in the macula.

We describe a patient with congenital hypertrophy of the retinal pigment epithelium (CHRPE) in the macular area of the right eye. The patient had no ocular complaints. Only relative central scotoma and a tritan opic defect were demonstrated in the affected eye, suggesting that CHRPE in the macula had hardly affected the function of the cones in the diseased area.

Fluorescein Angiography↗
PubMed1996

Heterogeneity and uniqueness of ornithine aminotransferase mutations found in Japanese gyrate atrophy patients.

PURPOSE: To identify mutations in ornithine aminotransferase (OAT) in seven Japanese families with gyrate atrophy (GA), an autosomal recessive chorioretinal degeneration of the eye caused by a generalized biochemical deficiency in OAT; mutations in the OAT gene have shown a high degree of molecular heterogeneity. METHODS: DNA was prepared from patients' fibroblasts and analyzed by polymerase-chain-reaction amplification of the OAT gene sequence, denaturing gradient gel electrophoresis, and direct sequencing for identification of the mutations. RESULTS: Eight different mutations were identified in seven unrelated Japanese GA patients with hyperornithinemia, confirming the high genetic heterogeneity of this disease. Five of these mutations were new, including one causing a pyridoxine-responsive disease, and all eight mutations have been found only in Japanese GA patients. Consistent with some similarity between the Japanese and Finnish populations in genetic isolation and homogeneity, there was a preponderance of homozygous mutations (five out of seven patients) as was previously reported for 16 Finnish GA pedigrees. CONCLUSION: The eight Japanese OAT mutations represent a group of heterogenous mutations unique to a specific population pool.

Amino Acid Sequence↗
PubMed1996

Postnatal developmental expression of glutamine and related amino acids in the rat retinas.

PURPOSE: To evaluate postnatal developmental changes in the amounts of retinal glutamate, glutamine and GABA, and in the distribution of retinal glutamine in the rat. METHODS: Free amino acids were extracted from rat retinas of different postnatal stages, and the concentrations of glutamate, glutamine and GABA were determined by HPLC. Also, anti-glutamine antibody was raised and an immunocytochemistry was performed with paraffin-embedded retinal sections in parallel with free amino acid analyses. RESULTS: Glutamate occurred in high concentrations at the birth and showed a stable pool, while glutamine and GABA remained low until postnatal day 3 or 5, and gradually increased in the developing rat retinas. Glutamine immunolabeling was observed in the retinal pigment epithelium and in a subpopulation of presumed amacrine cells in the early postnatal days. It was also found in Muller cells and in some ganglion cells or displaced amacrine cells in the ganglion cells layer. Glutamine immunolabeling was transiently observed also in horizontal cells. Finally, the immunolabeling was dominant in the inner and outer plexiform layers in the adult retinas. CONCLUSIONS: Postnatal developmental increase in the levels of glutamine and GABA might be dependent on the maturation of neurons or glial cells that possess the activity of the key enzymes of each amino acid. It was suggested that an expression of glutamine immunolabeling can be a marker of neurons that utilize glutamine as a precursor for glutamate or GABA, and of Müller cell maturations in postnatal early stage of the retina, while it changes to demonstrate the locations of glutamine cycle in the retina with adult characteristics.

Animals↗
PubMed1996

[Advantages and disadvantages of fast fluid-attenuated inversion recovery sequence in the evaluation of brain infarction].

This study was performed to determine the advantages and disadvantages of fast fluid attenuated inversion recovery (fast FLAIR) images in diagnosing brain infarction compared with fast spin-echo (fast SE) images. Fast FLAIR and fast SE images were obtained in 32 patients with brain infarction. Infarctions close to the sulci were difficult to differentiate from the real sulci on fast SE images, but were clearly depicted on FLAIR images. Linear foci along with cortex were especially well demonstrated on FLAIR images. On the other hand, foci that showed high signal intensity on fast SE images were sometimes appeared as having low or iso signal intensity on FLAIR images. These foci, suspected of being cystic lesions, were sometimes difficult to identify on fast FLAIR images. For the above reasons, we concluded that fast FLAIR and fast SE each have advantages and disadvantages in the detection of brain infarctions.

Cerebral Infarction↗
PubMed1996

[Lumbar MR myelography in the prone position: evaluation of nerve root sleeve delineation].

Although MR myelography is able to depict the subarachnoid space noninvasively, its ability to depict the nerve root sleeve is poor. The authors placed subjects in the prone position to increase the volume of spinal-fluid within nerve root sleeves and improve their depiction in MRM. MRM images of five normal volunteers taken in the supine and prone positions were visually correlated. Improved delineation of nerve root sleeves was obtained in all subjects.

Adult↗
PubMed1995

Autosomal dominant cone-rod dystrophy with negative electroretinogram.

AIMS: The negative electroretinogram (ERG) is observed in many hereditary retinal disorders. However, no reports have described a negative ERG in a family with autosomal dominant cone-rod dystrophy. A Japanese family with autosomal dominant cone-rod dystrophy with negative ERG is described. METHOD: Members of a Japanese family with autosomal dominant cone-rod dystrophy were examined and evaluated with Goldmann and Humphrey perimetry, bright flash ERG with an intense white stimulus, rod, cone, and flicker ERGs, and fluorescein angiography. Molecular analysis of the rhodopsin and peripherin/RDS genes in the patients was also performed. RESULTS: A 45-year-old Japanese man (proband) presented with decreased visual acuity. His fundi revealed bull's eye maculopathy and his single flash bright ERG showed a negative configuration. Negative ERG responses also were found in his father, who had macular degeneration, and one of the proband's three children who showed no fundus changes. No irregularities were found in their rhodopsin or peripherin/RDS genes. CONCLUSION: The condition of this family is believed to represent a previously undescribed autosomal dominant cone-rod dystrophy.

Adolescent↗
PubMed1995

X linked ocular albinism in Japanese patients.

Thirteen affected Japanese male patients and 13 female carriers with X linked ocular albinism from seven families were examined to assess their clinical findings and to compare them with those of white and black patients. Affected Japanese patients had poor visual acuity, horizontal nystagmus, macular hypoplasia, and loss of stereopsis. Some affected patients had non-albinotic fundus with moderate pigmentation. The amount of pigment in the fundus varied among affected patients and appeared to be between that of the white and black patients. All affected patients had brown irides that show no translucency. Interestingly, two affected patients had megalocornea and a third affected patient had posterior embryotoxon. All female carriers exhibited good visual acuity, normal eye position, stereopsis, brown irides without translucency, and the typical mosaic pattern in the fundus. The pigmented iris and fundus made the correct diagnosis of these affected patients difficult. Nine affected patients (70%) had been diagnosed initially as having congenital nystagmus, with or without macular hypoplasia, until they were reviewed for this study.

Adolescent↗
PubMed1995

An autopsied case of T-cell rich B-cell lymphoma with general involvement.

Autoptic findings of a 77-year-old man with T-cell-rich B-cell lymphoma (TCRBCL) showed predominant infiltration of reactive T-cells with a minority population of neoplastic B-cells in liver, spleen, pancreas, adrenal gland, stomach, small intestine and heart, as well as, lymph node. DNA studies demonstrated dual rearrangement in the T-cell receptor beta (TCR beta), immunoglobulin heavy chain J region (IgJH) and kappa light chain J region (IgJ kappa) genes.

Aged↗