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Biomedical subjects

T Shimoda

Publications and source records attributed to T Shimoda.

At least 235 records · Page 13Linked to original sources
PubMed1983

[Glucagon-producing carcinoid of the rectum--report of a case].

A 22-year-old male who had been receiving insulin therapy to treat uncontrollable hyperglycemia, was diagnosed to have a carcinoid in the lower rectum. The excised tumor was 1.5 cm in diameter, and showed a yellowish-white cut surface. It did not invade into the proper muscle. Numerous glucagon immunoreactive cells were found. The tumor cells contained many rounded neurosecretory granules. After tumor excision, the patient's hyperglycemia did not return in the absence of insulin administration. We suggest that the tumor may have secreted glucagon which may have acted as an antagonist against insulin in the circulation.

Adult↗
PubMed1983

[Clinical and pathological studies of multiple primary carcinoma of the colon and rectum].

During a 5-year period from 1976-1981, we clinicopathologically studied 21 patients with 45 lesions of multiple primary colorectal carcinomas and compared our findings with those made in 288 single carcinomas of the large intestine. The frequency of multiple primary colorectal carcinoma was 6.8%. The pathological findings suggested an interrelationship between colorectal adenoma and carcinoma. Patients with multiple carcinomas of the large bowel had coexisting adenomas more than patients with single carcinoma. We suggest that it is necessary to do a thorough preoperative examination of cancer of the large intestine to detect double cancers or other adenomatous polypoid lesions with synchronous association.

Adenoma↗
PubMed1981

Immunoperoxidase study of alkaline phosphatase in testicular tumor.

Indirect immunoperoxidase staining was carried out on human testicular tumors using monospecific antibodies against placental (Regan) and intestinal isoenzymes of alkaline phosphatase (ALPase). The very high incidence of seminoma (approximately 90%) revealed positive staining of placental ALPase mainly on the cell membrane of tumor cells, whereas none of the seminoma showed presence of intestinal isoenzyme. Placental isoenzyme was not recognized in any embryonal carcinoma and interstitial cell tumor. The epithelial cells of the glandular elements of teratoma occasionally exhibited strong staining for intestinal ALPase and weak staining for placental ALPase. The appearance of Regan isoenzyme in seminomas might be considered possible conversion of hepatic to placental isoenzyme, a consequence of malignant transformation of spermatogenic cells. Regan isoenzyme appears to be a new tumor marker for seminoma and the frequent identification of Regan isoenzyme in seminoma may disclose a unique biologic characteristic of this germinal tumor.

Adult↗
PubMed1981

Detection of HBsAg in the pancreas.

Hepatitis B surface antigen (HBsAg) has been reported to be present in other organs than the liver. 3,9 So far as our knowledge is concerned, however, any report of cases dealing with pancreatic diseased induced by hepatitis B virus (HBV) has not been described in the English and Japanese literature. We report an autopsy case with a pancreatic lesion characterized by damage of both exocrine and endocrine epithelial cells with inflammatory responses, which were immunohistochemically found to be positive for HBsAg, and electron-microscopically to possess core-like particles in the nucleus and cytoplasm.

Chronic Disease↗
PubMed1981

Light microscopic localization of hepatitis B virus antigens in the human pancreas. Possibility of multiplication of hepatitis B virus in the human pancreas.

Hepatitis B virus has been considered to be strictly organotropic and to infect and multiply only the hepatocytes of humans and chimpanzees. The localization of hepatitis B surface antigen in extrahepatic tissues has been regarded as due to deposition or phagocytosis of hepatitis B surface antigen circulating in the blood. In the present study, however, we demonstrated hepatitis B virus antigens in the pancreases of autopsied subjects with hepatitis B surface antigenemia by Shikata's orcein stain, and immunoperoxidase, immunofluorescent studies; hepatitis B surface antigen and hepatitis B virus core antigen were localized within the cytoplasm of pancreatic acinar cells in 18 and 6 cases, respectively, out of 30 cases studied. In contrast, 25 autopsy cases with no hepatitis B surface antigenemia failed to stain hepatitis B surface antigen or hepatitis B core antigen in the pancreas and liver. Therefore, it may be reasonable to assume that hepatitis B virus can infect and replicate in the human pancreatic acinar cells; however no convincing hepatitis B virus-associated ultrastructures were detected in the present study. Although there were some cases demonstrating chronic inflammatory reaction or fatty necrosis, or both, in the pancreas with hepatitis B virus antigens, the causal relationship between these pathologic changes and hepatitis B virus infection awaits further clarification.

Adult↗
PubMed1980

A 6-year-old boy having hepatocellular carcinoma associated with hepatitis B surface antigenemia.

Hepatocellular carcinoma associated with hepatitis B virus (HBV) infection occurring in a boy who was 6 years 2 months old is reported. The patient is thought to have been infected with HBV by exchange blood transfusion (horizontal infection) in the neonatal period. Jaundice appeared eight months after birth. He was subsequently treated and follow-up with a diagnosis of hepatitis B surface (HBs) antigen (Ag)-positive chronic persistent hepatitis. His mother and other family members were HBsAg-negative. Postmortem examination revealed a multinodular growth of hepatocellular carcinoma of trabecular and solid pattern, mainly of the right lobe, complicated with mild fibrosis of the liver. Numerous HBsAg-positive hepatocytes demonstrated by orcein staining and the indirect immunoperoxidase method were present in the noncancerous area. This hepatocellular carcinoma is considered to have occurred within six years two months after HBV infection and not to have been associated with liver cirrhosis. The present case may be valuable for assessing the oncogenic properties of HBV and the incubation period of HBV infection before it develops into hepatocellular carcinoma.

Carcinoma, Hepatocellular↗
PubMed1980

A light and electron microscopic study with comments on their relation to malignant fibrous histiocytoma and angiosarcoma.

Analysis of 365 cases of malignant soft tissue tumors revealed 83 cases of liposarcoma. The ages of the patients were distributed between 18 and 86 years with a mean average of 54.3 years. Approximately 50% of the cases were located in the lower extremity. Histologically, they consisted of 5 types; well differentiated (17 cases), myxoid (49 cases), round cell (3 cases), pleomorphic (11 cases), and mixed (3 cases). The appearance of lipoblasts of either the signet-ring type or mulberry (multivacuolated) type with displaced nuclei was the common characteristic feature for all types. Electron microscopically, lipid droplets were found inside of endoplasmic reticulum in lipoblasts, and abundant glycogen granules were seen in the cells containing only few lipid droplets. The lipoblasts were usually located close to the vascular wall in the interstitium, and findings suggesting an intimate relation between pericytes and lipoblasts were encountered. Although the differential diagnosis of malignant fibrous histiocytoma and liposarcoma was made possible by the appearance of lipoblasts in the latter, storiform pattern and histiocyte-like cells sometimes appeared in poorly differentiated liposarcoma. This tendency was also found in angiosarcoma. A close follow-up of recurrent cases revealed that there are actually cases of liposarcoma with malignant fibrous histiocytoma-like pattern and angiosarcoma with malignant fibrous histiocytoma-like pattern. It was considered that malignant fibrous histiocytoma-like figures can appear in various poorly differentiated sarcomas.

Adult↗
PubMed1980

Cytoplasmic blood plasma inclusions of canine hepatocytes demonstration by immunoperoxidase-labeling method.

Cytoplasmic inclusions in canine hepatocytes were scattered abundantly throughout hepatic lobules. They were mainly round, measured 3 microns to 20 microns in diameter, stained eosinophilic with hematoxylin and eosin stain, and were positive for diastase-resistant periodic acid-Schiff and phosphotungstic acid hematoxylin stains. Immunoperoxidase staining revealed that most of the inclusions were positive for such blood plasma components as albumin, immunoglobulins G, M, A, and transferrin. Ultrastructurally, the inclusions were granular, floccular, fibrillar, or homogeneous with a low or high electron density. Fibrin fibers and erythrocytes were occasionally recognized in the inclusions. These inclusions were considered to have derived from engulfment of blood elements into hepatocytes, probably as a result of increase of the intrasinusoidal blood pressure.

Animals↗
PubMed1980

Hepatic fibrosis. The relation between proliferating bile ductules and alpha1-antitrypsin.

Since there is a remarkable increase in connective tissue around the proliferating bile ductules in chronic hepatitis and liver cirrhosis, the participation of proliferating bile ductules in hepatic fibrosis has been discussed by many during the past. With the use of immunofluorescent method, the present authors have recently succeeded in detecting alpha1-antitrypsin, a protease inhibitor, in the epithelial cells of proliferating bile ductules and a portion of the hepatocytes connected with these epithelial cells. When considering the posibility of alpha1-antitrypsin being secreted into the interstitial tissue, it is conceivable that this glycoprotein plays an important role in restraining collagenase activity, which takes part in the degradation of collagen, and leads to abnormal proliferation of collagen.

Bile Canaliculi↗
PubMed1979

Establishment of a new human chondrosarcomatous cell line.

A new cultured cell line (HuOS) was established from tumor cells obtained from the pulmonary metastatic foci of a patient diagnosed as chondroblastic osteosarcoma. The tumor cell line was maintained for over 19 months, and morphological and biological characteristics were studied. These cells retained their malignant properties and produced nodules when transferred intramuscularly to nude mice. Morphologically, these nodules revealed a chondromatous pattern.

Adolescent↗
PubMed1979

Tissue distribution of human alpha1-microglobulin.

Human alpha(1)-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. The carbohydrate content was 21.7%. Anti-alpha(1)-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified alpha(1)-microglobulin, as well as to normal human serum and urine. Sera from the domestic chicken, mouse, rat, rabbit, dog, calf, cow, goat, sheep, and horse, however, did not react to anti-alpha(1)-microglobulin serum in immunodiffusion. The lymphocyte culture supernate was found to contain alpha(1)-microglobulin. Both thymus-derived(T)- and bone marrow-derived(B)-lymphocyte culture media clearly displayed a specific precipitin line against anti-alpha(1)-microglobulin serum when tested with the Ouchterlony immunodiffusion method. The tissue distribution of alpha(1)-microglobulin was studied under immunofluorescence, and a positive staining was recognized on the lymphocyte surface. Identical staining patterns were noted on both T and B lymphocytes, though B lymphocytes took a more intense stain. It would thus seem quite possible that lymphocytes are the primary source of alpha(1)-microglobulin and that this is filtered through the glomerular basement membrane and partly reabsorbed by the renal tubules. This, then, would suggest the possibility that alpha(1)-microglobulin shares some immunological role in vivo with lymphocytes and(or) is one of the membrane proteins of lymphocytes.

Alpha-Globulins↗
PubMed1978

[Clinicopathological study of diffuse carcinoma of stomach (author's transl)].

The biological behavior of ulcer type gastric carcinoma was studied on 114 cases of diffuse carcinoma (Borrmann's 4 type) and 262 cases of early like advanced carcinoma (including superficial spreading type). In both types of gastric carcinoma, the age distribution, location, ulcer with cancer focus and prognosis differed greatly. The early like carcinoma was speculated to have advanced maintaining the groos findings of early gastric carcinoma, and its location and associated ulcer were the same as the early ulcer type of carcinoma. The prognosis of this type of carcinoma was good, showing a figure of 70% in 3 year survival rate. On the other hand, diffuse carcinoma demonstrated diffuse extensive infiltration of tumor cells along the gastric wall, resulting in poor prognosis with a 3 year survival rate of almost 0%. Histologically, diffuse type of carcinoma showed lymphatic infiltration of tumor cells, and this is probably the main reason for the diffuse infiltration in this type of carcinoma. Diffuse carcinoma is, therefore, considered to be one special type of carcinoma having different biological behavior compared with the other ulcer type of carcinoma, and diffuse carcinoma is not the terminal stage of early like advanced carcinoma. There are three stages in diffuse carcinoma: 1. Infiltrative stage: wide spread infiltration of cancer cells through lymphatic channels (lymphangiosis carcinomatosa) 2. Edematous stage: soluble collagen appearing in gastric wall 3. Sclerosing stage: soluble collagen changing into insoluble collagen leading to marked thickening and stiffness of the gastric wall. This is the end stage of gastric diffuse carcinoma. It is difficult to explain that the marked fibrosis of gastric wall is a result to stromal reaction from tumor cell infiltration, since extensive fibrosis is found in areas without tumor cells and stiffness of the gastric wall occurs in a too short period of time. The production of abundunt soluble collagen is probably related to cancer cells.

Adenocarcinoma, Scirrhous↗