Biomedical subjects

T Shida

Publications and source records attributed to T Shida.

At least 109 records · Page 6Linked to original sources

PubMed1993

Structural analyses of synthetic immobile Holliday junction.

In order to study the structural properties of Holliday junctions, we designed and synthesized oligodeoxyribonucleotides to construct the models of the Holliday junctions with no sequence symmetry at the sites of branching called immobile junctions. Electrophoretic and spectroscopic analyses of the immobile junctions indicated that the Holliday junctions constructed from four deoxy 24-mers and four 18-mers were stable in 0.1 M NaCl at approximately 5 x 10(-6) M strand concentration at room temperature. Addition of MgCl2 to the solution of HJs resulted significantly in an increase in melting temperature of HJs.

Bacterial Proteins↗

PubMed1993

[The allergic reaction to acid protease released by Candida albicans].

The species of Candida albicans (C. albicans) are known to colonize mucocutaneous regions as part of the endogenous flora. C. albicans is also known as an important allergen in human allergic diseases. However, the major allergen of C. albicans has not been identified yet. Acid protease released from C. albicans (CAAP) cleaves IgA or the secretory component to enable C. albicans to colonize on the mucous membrane. In this study, using CAAP and crude Candida antigen as challenging antigens, we carried out conjunctival provocation tests (CPT) on 68 adult bronchial asthmatic patients who showed immediate positive skin reactions to crude Candida antigen. In several patients we performed serum IgE antibody (RAST), histamine release from peripheral leukocytes (HRT) and bronchial provocation tests (BPT) to CAAP. Six of the 8 patients who showed positive CPT to crude Candida antigen (75%) showed positive CPT responses to CAAP. On the other hand, only 1 of the 59 patients who showed negative CPT to crude Candida antigen (1.7%) showed positive CPT reactions to CAAP. Two female patients showed positive RAST and HRT to both crude Candida and CAAP. They also showed positive CPT and BPT. One patient had positive RAST results but negative HRT, CPT and BPT results. These results show that CAAP is an important allergen for atopic patients allergic to C. albicans.

Adult↗

PubMed1993

[A study of sensitization and symptoms in adult asthmatics who keep cat and dog].

We studied hypersensitivity to cats and dogs in three hundred adult asthmatics who kept them. Some findings were noted as follows. 1) The mean duration for which cats were kept was 6.2 +/- 10.1 (1 S.D.) years and that for dogs was 4.2 +/- 9.6 (1 S.D.) years. 2) The positive rate in intradermal tests to dog dander was 7% and to cat hair-epithelium 32%. The positive RAST (score > or = 2) rate to cat epithelium was 36.1%, to dog dander 31.6% and to dog epithelium 7.3% respectively. 3) Eleven to 18% of the dog keepers and 23 to 36% of the cat keepers whose RAST score were positive were aware of the relationship between pets and asthma, specifically concerning eye and nose symptoms. 4) Thirty percent of the cat and dog keepers had become sensitized to them after keeping them for under one year. The positive RAST rate was unchanged in those who kept Japanese native dogs but increased in those who kept foreign breeds after 3 to 6 years. The rate of sensitization rate to cats tended to increase along with the period for which they were kept. 5) Dog dander RAST scores among the foreign breed keepers were significantly higher than those among the native dog keepers. If the dog breed was the same, the RAST score was not higher if the dog was kept indoors than if it was kept outdoors. We concluded that a possible reason why the RAST scores were higher among those who kept foreign breeds was that foreign breeds have stronger allergenicity.

Adult↗

PubMed1993

[Immunological and physicochemical properties of Cry j II, the second major allergen of Japanese cedar pollen (Cryptomeria japonica)].

Cry j II, the second major allergen of Japanese cedar (sugi, Cryptomeria japonica) pollen was examined for the allergenicity by intradermal test and RAST. Nineteen of the 25 allergic patients examined, showed positive reaction to the Cry j II. Contents of Cry j II in the extracts of the pollen collected in various regions from 1977 to 1991 showed yearly variation ranging from 2.9 to 14 mg/100 g pollen, whereas the amount of Cry j I in the extract was comparatively stable at about 35 mg/100 g pollen. Physicochemical treatments of Cry j I and Cry j II suggested that specific human IgE antibodies and some mAbs bind to conformational epitopes which are denatured and destroyed by certain treatments.

Allergens↗

PubMed1993

[Changes in antigen-specific IgG, IgG4, and IgE antibodies in patients receiving immunotherapy with house-dust extract].

We measured allergen-specific IgG, IgG4, IgE antibodies and total IgE in sera from 64 patients who were receiving conventional immunotherapy (IT) with house dust (HD) extract. The sera were taken before and after IT. To measure allergen-specific antibodies, we used crude DF antigen, Der f I and Der f II for IgG and IgG4 antibodies, and crude DF antigen for IgE antibodies. The patients were divided into 3 groups: very short term group (IT period < 2 years, n = 9); short term group (2 < = IT period < 7 years, n = 25); long term group (7 years < = IT period, n = 30). The specific IgG antibodies for DF crude antigen did not change in any group. The specific IgG antibodies for Der f I and Der f II increased in the short term group and in the long term group. The specific IgG4 antibodies for all three antigens increased remarkably in the short term group and in the long term group. In the very short term group, however, the specific IgG4 antibodies did not increase for any antigen. Total IgE and the specific IgG antibodies did not change through IT. The specific IgG4 antibodies increased significantly through IT, but the time course of the increment of the IgG4 antibodies was not parallel with the clinical course of IT.

Adult↗

PubMed1993

[A case of left atrial ball thrombus].

A 76-year-old man admitted to one hospital complaining of transient left hemiparesis. Because of arrhythmia, he was examined by an echocardiogram and found to have an oval free-floating thrombus in his left atrium concomitant with mitral stenosis. He was transferred to our hospital for emergency surgery. On operation, a 3 x 4 cm ball thrombus was removed and diseased mitral valve was replaced with a SJM prosthetic valve. His postoperative course was relatively uneventful. Though left atrial ball thrombus is rarely found among the patients with mitral valve disease, when left atrial ball thrombus is found, immediate surgical intervention is recommended to avoid sudden death.

Heart Atria↗

PubMed1993

[A successful VA bypass treatment in patient with severe pulmonary hypertension].

Report of a case of a patient with pulmonary hypertension associated with pericardial effusion who was treated by VA bypass during life-threatening right ventricular failure. A 63-year-old man was admitted to our hospital complaining of chest pain and dyspnea. Echocardiography revealed the patient had severe pulmonary hypertension and pericardial effusion. Though there were deep Q waves in ECG, his coronary angiography revealed normal coronary arteries. Pulmonary scintigraphy showed no evidence of pulmonary thromboembolism. As he developed hypotension and anuria, emergency pericardial drainage was carried out without any beneficial effect. Because no effective drugs were found to control his systemic hypotension and pulmonary hypertension, he was treated by VA bypass to save his life. Although his systemic pressure rose to normal level following institution of VA bypass, his pulmonary pressure remained high constantly, around 60-70mmHg. The VA bypass was discontinued on the 6th day because of bleeding tendency. 3 months after VA bypass, though he had not been given any drugs to control pulmonary hypertension, his pulmonary arterial pressure dropped down to within normal range. Though the exact mechanism or etiology of pulmonary hypertension remained unclear, this may be a rare case treated by VA bypass successfully for life-threatening right ventricular failure caused by pulmonary hypertension.

Extracorporeal Membrane Oxygenation↗

PubMed1993

[A study of characteristics in adult asthmatics who are sensitized by cats and dogs allergens].

We studied adult asthmatics who were sensitized (RAST sore > or = 1) by cats and dogs allergens. Compared with non-sensitized asthmatics, the following some characteristics were noted in the sensitized asthmatics: 1) The histogram of the onset age of asthma showed two peaks. 2) The asthmatics sensitized by cats epithelium were more frequent and had higher RAST scores than those sensitized by dog hair dander. 3) There was a significant correlation between the RAST scores to dogs dander and dog hair. 4) There was no correlation between the RAST score to cat epithelium and dog hair. 5) The positive rations of intradermal testing to other allergens were higher in the sensitized asthmatics than in the non-sensitized asthmatics, especially to some foods allergens. 6) The asthmatics sensitized by cat and dog allergens were much more sensitized by house-dust mites than were the non-sensitized asthmatics. The asthmatics sensitized by cat and dog demonstrated being allergic to not only cat and dog allergens but also to many other allergens. We thought that the sensitized asthmatics were more atopic than the non-sensitized asthmatics and this was the reason why they were sensitized by cat and dog. The RAST scores to cat allergens were higher than the RAST scores to dog allergens. This fact showed that the cat was more important than the dog. This result agreed with our clinical experience.

Adolescent↗

PubMed1993

[Advance in the method of eosinophil separation--separation of human eosinophils in density gradients and measurement of density distribution].

To separate human eosinophils from peripheral blood, we used six discontinuous density gradients of Percoll solution (1.070, 1.080, 1.085, 1.090, 1.095 and 1.100 g/ml). Eosinophils isolated from patients with bronchial asthma or hypereosinophilic syndrome (HES) were shifted to the lighter density as compared with that from healthy subjects. Light-shift of density of eosinophils (hypodense eosinophils) was mimicked by several agents including chemical mediators such as histamine or platelet-activating factor (PAF), cytokines such as interleukin (IL)-3, IL-5 or GM-CSF. It has been reported that hypodense eosinophils survive, and the ability to generate leukotriene C4, superoxide anion or to secrete tissue injurious protein such as ECP or MBP augments as compared with that of normal density (normodense eosinophils). This separation method of eosinophils using a Percoll solution may contribute to accurate understanding a role of eosinophils in the pathogenesis of bronchial asthma or HES.

Animals↗

PubMed1993

[Specific IgE antibody titers to hen's egg white lysozyme in allergic children to egg].

Hen's egg white lysozyme (HEL) is one of the minor allergen in hen's egg white. HEL is commonly used to treat disease of respiratory tract, because it have the effect to dissolve mucopolysaccharide and anti-inflammatory action. We examined specific IgE antibody titers (IgE-HEL) in patients with egg allergy and allergic patients to other antigen than egg. Results indicated that 16.37 +/- 29.56 (PRU/ml) (mean +/- SD) of IgE-HEL was found in 30 out of the 39 allergic patients to egg, and 23 (66.7%) out of the 39 patients studied showed RAST scores of more than 2. On the other hand, 1.08 +/- 0.92 (PRU/ml) of IgE-HEL in 12 out of the 44 allergic patients to other antigen than egg, and 5 (11.4%) out of the 44 patients studied showed RAST scores of more than 2. Moreover, we treated a patient who developed anaphylaxis after taking HEL. 1.0 (PRU/ml) of HEL-IgE was found in this patient. These results suggest that we should be careful in treating allergic patients with HEL.

Adolescent↗

PubMed1992

Late radiation response of canine mediastinal tissues.

The mediastinal tissues which included heart, lung, trachea and esophagus of 70 adult beagle dogs were irradiated to a range of total radiation doses between 24 and 68 Gy given in 2, 3 and 4 Gy fractions. The purpose of the study was the calculation of alpha/beta ratios for morphologic and functional changes of the mediastinal tissues. Functional assays including echocardiography, electrocardiography, right heart hemodynamics and cardiac output were performed. Histomorphometric analyses of all tissues included in the field were done 2 years after treatment. Euthanasia was performed on 7 of 70 dogs prior to 2 years due to congestive heart failure and seven other dogs had signs of heart failure 2 years after treatment. Heart failure was thought to be caused by either pericardial effusions or constrictive pericarditis in these dogs. Heart failure occurred at doses of 62 and 68 Gy given in 2 Gy fractions, 60 Gy given in 3 Gy fractions and 52 Gy given in 4 Gy fractions. The ED50 values for pericardial fibrosis for 2, 3 and 4 Gy fractions were 46.1, 43.9 and 26.6 Gy, respectively. An alpha/beta ratio of 2.5 Gy was calculated by direct quantal response analysis. Small foci of myocytolytic lesions were detected in 11 dogs. Calculated ED50 values for myocytolysis were 70.4 Gy given in 2 Gy fractions and 50.8 Gy given in 4 Gy fractions. The estimated alpha/beta ratio was 3.2 Gy. Heart rates determined from physical examination and frequency of S-T segment changes increased with increasing dose. No other dose related changes were found in any of the other functional parameters. Functional changes were detected in the 14 dogs with clinical signs of heart failure. Focal consolidation and subpleural fibrosis were present in the irradiated lung volume. These late changes had no detectable physiologic effect in these dogs because of the small volume of lung irradiated. The ED50 values for lung consolidation were 54.3, 45.8 and 26.6 Gy after 2, 3 or 4 Gy fractions, respectively. The estimated alpha/beta ratio was 3.4 Gy. No dose-related changes could be detected in the trachea or esophagus at 2 years after treatment. These results demonstrate that lung and pericardium are the most responsive tissues in the mediastinum within the first 2 years after treatment. Myocardial lesions were present with high ED50 values, but were not found to be functionally significant at 2 years after irradiation. Human clinical data indicate that longer observation periods are needed for development of these lesions.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

PubMed1992

Concentration of airborne mite allergens (Der I and Der II) during sleep.

Using a low-noise air sampler and a sensitive radioimmunoassay, we measured the concentration of mite allergens in the air during sleeping with Japanese bedquilts (futon). The airborne allergen levels of Der I (Der p I plus Der f I) and Der II during sleep were 223 and 87.1 pg/m3 of air, respectively. These levels were about 10-fold higher than those during usual domestic life in the living room of the same houses. When the bedquilts were changed to new ones free of mite allergens, the airborne allergen levels of Der I and Der II were decreased to 11.5 and 12.0 pg/m3, respectively. This indicated that the mite airborne allergens during sleep were generated from the used bedding, not from the floor. We believe that exposure to airborne mite allergens during sleep might be an important factor in the development of mite allergies.

Air Pollution, Indoor↗

PubMed1992

Establishment of a sensitive radioimmunoassay for the detection of human IgE-binding factor (soluble CD23).

A monoclonal antibody (mAb) specific to low-affinity receptor for IgE (FceRII/CD23) was established by the fusion of spleen cells of BALB/c mice immunized with the FceRII+ human B lymphoblastoid cell line (RPMI 8866) with mouse myeloma P3U1. Four mAbs, 10/3 (IgG1), 11/4 (IgG1), 12/2 (IgG2b) and 15/6 (IgM), almost completely inhibited the IgE binding to FceRII+ cells but not to FceRII- cells. More directly, they were demonstrated to react only with 43-kD component/FceRII of the cell lysate of RPMI 8866 cells by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis. Since they have a different epitope specificity, a solid-phase radioimmunoassay (RIA) for the measurement of IgE-binding factor (IgE-BF) was established. It was found that the RIA with the use of 10/3 and 125I-labeled 11/4 or 12/2 gave good results in the detection of IgE-BF derived from B cells and monocytes as well as of T-cell-derived IgE-BF. More importantly, serum IgE-BF was also quantitatively measured by this RIA. Although increased serum levels of IgE-BF were observed in atopic patients, serum IgE-BF was decreased rather than increased in patients with very high serum IgE. This phenomenon may be explained by the decreased ability of the patients' B cells to spontaneously release IgE-BF in vitro.

Animals↗

PubMed1992

Characterization of four-stranded DNA and RNA fragments.

We have investigated the structures formed by deoxyribo- and ribooligonucleotides containing guanine-rich sequences. Inter- and intrastrand guanine tetrads are not only more stable in the presence of K+ than they are in the presence of Na+, but also more compact.

DNA↗

PubMed1992

[A new steroid therapy for difficult asthmatics--an induction and maintenance, two-step therapy].

Beclomethasone dipropionate (BDP) administered by inhaler is a very useful drug for the treatment of bronchial asthma. In this therapy, it is very important to use steroids systematically to induce a complete remission of asthma attack (first step) and then begin to use BDP a dose of more than 800 micrograms to maintain remission (second step). We treated 27 difficult asthmatics with this therapy and found this new method very useful. The characteristics of asthmatics were as follows. 1) The age ranged from 37 to 82, and the mean age (+/- S.E.) was 58.9 (+/- 2.6) years old. 2) The onset age ranged from 27 to 74 with a mean age (+/- S.E.) of 46.9 (+/- 2.6) year old. 3) The number of non-atopy was 22 cases. 4) The follow-up duration ranged from 5 to 45 months with a mean (+/- S.E.) of 15 (+/- 2.0) months. The results were as follows. 1) The complete remission rate was 48%, partial remission 37% and unchanged 15%. 2) There was a significant increase only in %VC. 3) The peripheral eosinophil count was decreased significantly. 4) The log value of PC20 concentration by acetylcholine increased significantly by a factor of 2.94 to 3.28. 5) After this therapy, the mean serum cortisol level at 9:00 a.m. was 10.1 (+/- 3.8, S.E.) micrograms/ml. There were only 2 cases whose cortisol level were under the normal. 6) There were many oral side effects, namely stomatitis with 5 cases and hoarseness with 8.

Adult↗

PubMed1992

[A case of obstructive ileus following the upper Gi barium swallow study with condensed barium due to severe constipation].

A 64-year-old woman visited my clinic with complaints of abdominal distension, constipation, and nausea. Since abdominal X-ray showed severe obstructive ileus following the upper GI barium swallow study, the patient was sent immediately to Saga Medical School Hospital for the emergency operation. As the result of the left hemicolectomy, two obstructive lesions were found at the sigmoid colon and the transverse colon. Nowadays, as the population of aged people increases and Japanese diet changes, colon cancers are increasing more rapidly. Since the half of colon cancers doesn't have any clinical symptoms of obstruction, the upper GI barium swallow study with condensed barium might lead a patient to some type of dangerous situation.

Adenocarcinoma↗

PubMed1992

[A successful case of tricuspid valvulectomy for Ebstein's anomaly with severe tricuspid stenosis].

A 44-year-old male was admitted to our hospital complaining of general fatigue and dyspnea on exertion with congestive heart failure (NYHA IV). Cardiac catheterization and angiogram revealed Ebstein's anomaly with severe tricuspid stenosis and functioning small right ventricle. Because of tiny right ventricular chamber, the usual surgical treatment for Ebstein's anomaly i.e. tricuspid valve replacement or valvuloplasty were deemed to be inappropriate. Tricuspid valvulectomy was performed to relieve the inflow stenosis of the right ventricle and excellent symptomatic relief was achieved. It is very rare to seen an adult case of Ebstein's anomaly with severe tricuspid stenosis and functioning small right ventricle. Tricuspid valvulectomy may be justified to use in this rare condition.

Adult↗

PubMed1991

Establishment of Epstein-Barr virus-negative diffuse large cell lymphoma cell line with an 8;22 chromosomal translocation.

A new human B-cell lymphoma cell line was established from a pleural effusion of a patient with a diffuse large cell lymphoma which originated from an ileocecal tumor. The cell line, designated KAL-1, has been passaged 280 times over a period of 22 months. This cell line was successfully maintained in a chemically defined serum-free medium; its doubling time is approximately 24 h. Immunologically, the cells were demonstrated to express IgM lambda on the cell surface and to react with monoclonal antibodies to B-cell antigen including B1, B4, HLA-DR, and common acute lymphoblastic leukemic antigen but not with B2 and all the T-cell markers. Immunoglobulin gene analysis revealed rearrangements of both JH and C lambda. These data indicate that this cell line represents the B-cell lineage at the immature B-cell stage. This cell line was negative for Epstein-Barr virus nuclear antigen and had no detectable Epstein-Barr virus genome in cellular DNA. Chromosome analyses revealed that the cells carried an 8;22 chromosome translocation, reminiscent of variant type Burkitt's lymphoma. However, there was no histological evidence for Burkitt's lymphoma. Molecular studies showed that KAL-1 had deregulated high constitutive expression of c-myc. This cell line was demonstrated to be highly tumorigenic when injected into athymic nude mice. This tumor model should provide clues about the molecular mechanism involved in the pathogenesis of B-cell malignancy and appears to be a useful in vivo model for the study of molecular events during B-cell differentiation and therapeutic investigations.

Adult↗