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Biomedical subjects

T Shibata

Publications and source records attributed to T Shibata.

At least 901 records · Page 50Linked to original sources
PubMed1987

Maintenance of myeloid leukemic cells in the adherent layers of long-term bone marrow cultures.

In order to determine the culture characteristics of human leukemic cells in long-term bone marrow cultures (LTBMC), we cultured three specimens from three patients with acute non-lymphoblastic leukemia (ANLL) at diagnosis or during relapse. The kinetics of granulocyte-macrophage colony-forming cell (CFU-C) generation in non-adherent cell fractions were compared with the kinetics of CFU-C generation by 16 control specimens. In addition, the adherent cells of the three ANLL specimens were cytogenetically characterized and the karyotypes were compared with those of corresponding fresh specimens. Two of the three ANLL specimens showed a greater decline in the number of CFU-C by weekly CFU-C assay of non-adherent fractions than did the control specimens at the corresponding week of culture. At the end of LTBMC, these two specimens contained karyotypically identifiable leukemic cells in the adherent layers. In contrast, one of the three specimens generated no CFU-C during the first four weeks of culture, but CFU-C were generated between weeks 5 and 9 with a peak at week 8. This specimen's adherent layers contained no metaphases identifiable as leukemic cells, even although the fresh sample before culture had yielded abnormal metaphases in 100% of the cells examined. This biological heterogeneity of ANLL leukemic cells may be significant in understanding the marked variability in the clinical courses of ANLL patients. A large prospective study that incorporates LTBMC would be of value in determining the clinical correlations.

Bone Marrow↗
PubMed1987

Distribution of pancreatic secretory trypsin inhibitor in various human tissues and its inactivation in the gastric mucosa.

Considerable amounts of immunoreactive pancreatic secretory trypsin inhibitor were detected in lung, stomach, liver, pancreas, kidney, small intestine and ovary. Immunoreactive pancreatic secretory trypsin inhibitor content of surgically removed gastric mucosa was approximately seven times higher than that of gastric mucosa obtained at autopsy. The reduction in PSTI-immunoreactivity seen in a tissue extract of fresh gastric mucosa on 24 hours' incubation represented 9% of the original immunoreactivity in 10 mM Tris-HCl buffer, pH 7.4, at 37 degrees C. While, there was a more than 80% reduction in immunoreactivity on 12 hours' incubation at 37 degrees C at pH 4.0 or under more acidic conditions.

Female↗
PubMed1986

Purification and characterization of pancreatic secretory trypsin inhibitor in human gastric mucosa.

Immunoreactive pancreatic secretory trypsin inhibitor (PSTI) was purified to homogeneity from human gastric mucosa by gel chromatography, ion-exchange chromatography, and repeated reverse-phase high-performance liquid chromatography (HPLC). The molecular weight of the purified immunoreactive PSTI in human gastric mucosa was estimated to be 6000. The electrophoretical mobility of purified PSTI was identical with that of the main component of PSTI in human pancreatic juice. It consisted of 56 amino acids and had the same amino acid composition as PSTI in pancreatic juice. It inhibited bovine pancreatic trypsin stoichiometrically, and did not inhibit porcine pancreatic kallikrein or elastase. Heat treatment of immunoreactive PSTI in gastric mucosa showed the same inactivation curve of immunoreactivity as that of pancreatic juice PSTI.

Amino Acids↗
PubMed1986

Development of a radioimmunoassay for human deoxyribonuclease I.

A reliable radioimmunoassay (RIA) for human deoxyribonuclease I (DNase I) is described. Using delayed addition of tracer antigen, the method is sensitive (9.5 reproducible and specific. A good parallel relationship was observed between the standard curve and dilution curves for human urine and human pancreatic juice. G-actin, a naturally occurring DNase I inhibitor, caused no change in the immunoreactivity of DNase I. In healthy individuals, aged 11-90 yr, the mean serum DNase I was 18.4 ng/ml (SD 6.7 ng/ml). Increased serum DNase I occurred in patients with acute pancreatitis, renal failure, and in about one-third of patients with various malignant tumors.

Actins↗
PubMed1986

A case of multicentric synchronous osteosarcoma treated with a vascularized tibia graft from an amputated limb.

A 10-year-old girl with synchronous lesions of osteosarcoma in the left proximal tibia and in the right distal radius but with no lung metastasis was treated by an innovative procedure. After an above-knee amputation and a wide excision of the distal halves of the radius and ulna, the skeletal defect of the forearm was replaced by the distal tibia harvested from the amputated leg using the microsurgical technique of revascularization. The result was encouraging up to the point of her death due to other, widespread lesions.

Amputation, Surgical↗
PubMed1986

Comparison between arthroscopic and open synovectomy for the knee in rheumatoid arthritis.

Ten surgical anterior capsulosynovectomies following Mori's four-block capsular incision technique in nine rheumatoid patients and 14 arthroscopic synovectomies utilizing a Wolf arthroscope with a large pituitary rongeur or a motorized intra-articular shaver in 11 patients were performed in our department. In three patients with bilateral involvement at nearly the same stage (III), we operated on the knees simultaneously, using open capsulosynovectomy on one side and arthroscopic synovectomy on the other side; we comparatively assessed the postoperative course, the subjective evaluation of the patients, and the follow-up results. Surgical intervention is milder in the arthroscopic operation, and postoperative knee pain during motion exercise is markedly less in the arthroscopically synovectomized knee. Although the postoperative management was more complex for open capsulosynovectomized knees, the results obtained at 1-2 months after synovectomy showed no significant difference between the two procedures.

Adult↗
PubMed1986

Chemical studies of pancreatic nonopaque concretions and protein plug.

Chemical studies were performed on all or part of four X-ray translucent (nonopaque) concretions and one protein plug to investigate a possible relationship between these substances, and to find the similarities and differences between these materials and pancreatic stone protein. Through elemental analysis, infrared absorption spectrometry, quantitative analysis of protein, and amino acid analysis, nonopaque concretions and protein plug were found to consist mainly of protein. One nonopaque concretion contained a very small amount of crystalline which differed greatly from calcite type calcium carbonate as observed in pancreatic calcified stones. The pattern of amino acid composition was very similar between the concentrations and the protein plug: rich in acidic amino acids, but poor in basic and aromatic residues. The aspartic acid content was the highest among detected amino acids. Comparison of amino acid composition between the nonopaque concretions which we analyzed and the pancreatic stone protein reported from Sarles' laboratory suggested that the concretions seem to contain some stone protein.

Adult↗