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Biomedical subjects

T Sakurai

Publications and source records attributed to T Sakurai.

At least 721 records · Page 40Linked to original sources
PubMed1985

Sulfated glycopeptides from middle ear effusions of secretory otitis media.

The middle ear effusion specimens were obtained by myringotomy and aspiration from 4 children of 4-7 years old, who had been diagnosed as patients with secretory otitis media on the basis of conductive hearing loss and tympanogram. In cases 1 and 2, their ear fluids were macroscopically serous, while those of cases 3 and 4 were mucous. These ear fluids were digested with pronase and the digests were analyzed by cellulose acetate membrane electrophoresis with alcian blue and high-iron-diamine stainings. All samples were found to contain glycopeptides possibly derived from sulfated mucin-type glycoproteins with small amounts of glycosaminoglycans. The glycoconjugates from cases 3 and 4 were further examined after hyaluronidase and chondroitinase ABC treatments, followed by heparitinase digestion. The resultant glycopeptide fractions appeared to be electrophoretically homogeneous and their chemical compositions suggested that they were typical mucin-type glycopeptides. Furthermore, they contained sulfates. The data suggest that in secretory otitis media, one of the major components of middle ear effusions is sulfated mucin-type glycoprotein.

Amino Acids↗
PubMed1985

[Combined chemotherapy for head and neck cancer using cisplatin, methotrexate and continuous subcutaneous infusion of peplomycin].

Twenty-one patients with advanced or recurrent squamous cell carcinoma of the head and neck were treated with cisplatin, methotrexate and continuous subcutaneous infusion of peplomycin. The overall response rate was 62% (13/21) with 19% (4/21) complete response. The median duration of partial response was 2 months, while that of complete response was 3 months. Toxic effects were acceptable with no fatalities, but nephrotoxicity (33%), leukocytopenia (24%), thrombocytopenia (29%) and severe nausea and vomiting (76%) occurred. Pulmonary toxicity due to continuous subcutaneous infusion of peplomycin (15 mg/day, 4 days) was not seen.

Adult↗
PubMed1985

[Tissue distribution of cefminox in beagle dogs].

A new cephamycin antibiotic, cefminox (MT-141, CMNX), was intravenously infused into Beagle dogs at a dose of 40 mg/kg in order to study it's distribution to various tissues. The following results were obtained. The maximum serum concentration of CMNX observed at the end of the infusion period was 102.3 micrograms/ml, and then the concentration decreased. The biological half-life of CMNX in serum was 37.0 minutes. This half-life was similar to the results of previous studies with Beagle dogs and rabbits. The maximum concentrations in tissues and body fluids were highest in B-bile followed by kidney, urinary bladder, serum, liver, vagina, uterus, pericardiac fluid, trachea, ovary, lung, gallbladder, parotid gland, heart, tonsil, thymus, spleen, pancreas, aqueous humor and cerebrospinal fluid, in that order and not detected in brain. The maximum concentrations in gallbladder, B-bile, pericardiac fluid and cerebrospinal fluid were found at 1-2 hours after administration. In other tissues and body fluids, they were obtained at the end of the infusion period. The area under the tissue concentration curve (AUC) was highest in the urinary bladder followed by the kidney, vagina, liver, uterus, gallbladder, trachea, ovary and lung, in that order. These results suggest that CMNX is useful for various infectious diseases in these tissues. The pharmacokinetic parameter (K1i/K2i) derived from serum and tissue concentrations using the deconvolution method well correlated to maximum tissue concentrations.

Animals↗
PubMed1985

[Management of vesicoureteral reflux in children with neurogenic bladder--retrospective studies of antireflux surgery].

During the last 3.5 years, 18 children and 9 adults with vesicoureteral reflux and neurogenic bladder dysfunction were seen at our hospital, and 11 children and 4 adults required antireflux surgery. The criteria of repair were progressive renal deterioration and/or uncontrollable urinary infection. Surgery was applied mainly to the conditions of detrusor hyperreflexia and/or lower urinary tract obstruction. The combined technique of Politano-Leadbetter and Glenn-Anderson was used in 8 children and 3 adults, and the Cohen technique was used in 3 children and 1 adult. Surgery was successful that is, cessation of reflux and no obstruction, was achieved in all but one patient, who had received radical hysterectomy previously. Though recurrent episodes of lower urinary tract infection persisted in some cases after operation, over-all usage of antibiotics was reduced. Seven children with reflux and uninhibited bladder, though some of them with high grade reflux, were managed successfully by anticholinergic drug or transcutaneous electrical stimulation.

Adolescent↗
PubMed1985

Beneficial effect of topical fibronectin in patients with keratoconjunctivitis sicca of Sjögren's syndrome.

Fibronectin, a high molecular weight plasma protein, was applied locally to 12 patients with keratoconjunctivitis sicca of Sjögren's syndrome refractory to conventional treatment. All patients had ophthalmologically proved keratoconjunctivitis sicca of unknown etiology and were suffering from severe eye symptoms. Fibronectin was purified from autologous plasma and administered to the patients as tear drops 3 times a day. All patients responded to the therapy with fewer subjective symptoms and decreased fluorescein staining. All but 3 responded to the therapy as judged by rose bengal test. No adverse effects were encountered. We conclude that local application of fibronectin may benefit patients with keratoconjunctivitis sicca of Sjögren's syndrome.

Fibronectins↗
PubMed1985

Significance of a gastric mass screening survey.

We analyzed the rate of occurrence of gastric cancer in a population of 39,250 by mass screening survey. In 0.123% of the total subjects gastric cancer was detected, 0.064% was at early stage and 0.059% at an advanced stage. The incidence of gastric cancer in the newly examined persons was 0.17%, 0.08% was at early stage and 0.09% at advanced stage. Of the frequently examined persons 0.07% was found to have gastric cancer, 0.043% at early stage and 0.027% at an advanced stage. The rate of occurrence of early gastric cancer was significantly higher than that of advanced gastric cancer within a 1 1/2 year interval of screening. From these results, gastric mass screening survey is considered to be effective in detecting asymptomatic gastric cancer in the "newly examined persons," and also to be beneficial in detecting "early" gastric cancer at intervals of 1 1/2 years of screening.

Adult↗
PubMed1985

[Interventricular septal configurations and motions in patients with hypertrophic cardiomyopathy by angled biventriculography].

Precise configurations and motions of the interventricular septum (IVS) in hypertrophied hearts were assessed using angled biventriculography (BVG) for eight patients with apical hypertrophic cardiomyopathy (AH), three with hypertrophic obstructive cardiomyopathy (HOCM), six with hypertrophic non-obstructive cardiomyopathy (HNCM) and four with hypertensive heart disease (HHD). Analysis was by the computer system, OSCON. The configurations of the IVS differed among the four groups: the thickness of the IVS increased gradually towards the apex in patients with AH; that of the upper IVS increased abnormally in patients with HOCM; that of the mid-IVS increased in patients with HNCM; and that of the IVS increased uniformly in patients with HHD. The segmental thickening of the IVS also differed in each of the four groups. Among them, a characteristic pattern of the segmental thickening of the IVS was evident in patients with HNCM. In addition, the relative contributions of the IVS to right and left ventricular contractions in patients with AH and HHD differed from those in patients with HOCM and HNCM. Briefly, IVS contractions contributed more to the left than to the right ventricle in patients with AH and HHD, but not in those with HOCM and HNCM. Although further study is necessary, it can be concluded that AH can be regarded as one type of hypertrophic cardiomyopathy, according to its IVS configuration and motion.

Adult↗
PubMed1985

[Results of the Burch colposuspension operation for female stress incontinence].

Of 48 patients with radiologically proven cystocele, 13 patients were treated with the Burch colposuspension procedure from October, 1980 to August, 1984 in our clinic. A satisfactory result with good urinary control was obtained in 13 cases and the procedure was a failure in one. Partial or complete urinary retention was evident postoperatively in 8 patients (62%), but all of the patients voided satisfactorily within 4 weeks. Radiological diagnosis of the cystocele and advantage of Burch colposuspension procedure are discussed.

Adult↗