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Biomedical subjects

T Saida

Publications and source records attributed to T Saida.

At least 145 records · Page 8Linked to original sources
PubMed1987

Expression of heterophile, Paul-Bunnell and Hanganutziu-Deicher antigens on human melanoma cell lines.

Expression of heterophile antigens was studied on 6 human melanoma cell lines. Paul-Bunnell and Hanganutziu-Deicher (H-D) antigens, but not Forssman antigen, were demonstrated on these cell lines. H-D antigen was also demonstrated on melanoma cells isolated from primary and metastatic lesions. Evidence was also presented that HLA class I but not class II (DR and DQ) molecules are expressed on these cell lines. H-D antibodies of IgG class were detected in 10 of 23 (42%) melanoma patients suggesting the possibility that H-D antigen might have been expressed in an immunogenic form in the patients.

Antibodies, Heterophile↗
PubMed1986

Sera from patients with multiple sclerosis react with human cell T lymphotropic virus-I gag proteins but not env proteins--Western blotting analysis.

To study the possible involvement of human T cell lymphotropic virus type I (HTLV-I)-related agent in Japanese multiple sclerosis (MS), we performed a Western blotting analysis, using purified viral antigens, on sera from 46 patients with MS, nine patients with other neurologic diseases, and 11 healthy controls. Of 46 MS patients, 11 (24%) had antibodies reactive with antigens corresponding to the group-specific antigen (gag) proteins (p15, p19, and p24), although the prevalence was lower than that reported in a recent study using an enzyme-linked immunosorbent assay (ELISA). Despite the lower frequency of immunoreactivity, Western blotting technique had merits of identification of multiple antigens and higher specificity for detection of antibodies than ELISA. Those sero-positive patients consisted of four cases with IgG antibodies reactive mainly to the gag p24 and/or p15, four with IgM antibodies mainly to the gag p24 and/or p19, and three with both IgG and IgM antibodies. These immunostaining patterns of MS sera were clearly distinguishable from those of adult T cell leukemia patients who had antibodies to the envelope (env) proteins and its precursors in addition to the gag proteins. The antibody in MS sera was generally of low titer and reactive at a high serum concentration (1/10 dilution). None of the sera from patients with other neurologic diseases and healthy controls had the viral antibodies. These findings indicate that at least one quarter of Japanese MS patients have antibody responses to a hitherto unidentified agent related to HTLV-I, which possibly plays a part, primarily or secondarily, in the pathogenesis of those patients.

Adolescent↗
PubMed1986

Proliferation of Schwann cells in demyelinated rat sciatic nerve.

Experimental demyelination was induced by intraneural injection of anti-galactocerebroside serum into the sciatic nerves of rats. Schwann cells undergoing mitotic division were observed between days 3 to 9 after the injection and demyelinated segments were still associated with macrophages. Dividing Schwann cells were often present in association with both unmyelinated and myelinated fibers. Whether or not, daughter Schwann cells migrate along the same fiber towards neighboring demyelinated segments remains unclear. When Schwann cells attached to axon membranes of demyelinated segments were studied at later time points, they were present in clusters randomly at various regions of the segments. There was no proximo-distal gradient for the wave of Schwann cell proliferation. Mean Schwann cell internuclear distances were around 40-50 microns at the earliest time of remyelination. Schwann cell redistribution and remyelination progressed regardless of the length of demyelinated segments.

Animals↗
PubMed1986

Experimental clioquinol intoxication in rats: abnormalities in optic nerves and small nerve cells of dorsal root ganglia.

Pathological abnormalities in the central (CNS) and peripheral nervous system (PNS) were produced in rats by daily administration of 300-400 mg/kg clioquinol for 7-40 days. The changes comprised axonal degeneration of optic nerve fibers and mitochondrial swellings in small nerve cells of dorsal root ganglia. There were occasional myelin splittings in the spinal nerve roots. No apparent changes were observed in the spinal cord or peripheral nerves. Similar changes were occasionally encountered to a lesser extent in control rats by restricting the diet and water to maintain the body weight comparable to the ranges of experimental animals. It seemed that not only clioquinol intoxication but nutritional deficiency also contributed, in part, to the production of these CNS and PNS abnormalities. The possibility of the presence of peripheral neuropathy in subacute myelooptic neuropathy is discussed.

Animals↗
PubMed1986

Role of nonspecific myelin destruction by delayed type hypersensitivity in primary demyelination.

To clarify the role of nonspecific myelin destruction mediated by delayed type hypersensitivity (DTH) in primary demyelination, DTH to tuberculin was induced within the endoneurium by intraneural injection of purified protein derivative (PPD) or sonicated Mycobacterium tuberculosis into the sciatic nerves of Lewis rats and guinea pigs which had previously been sensitized to tuberculin. The morphological features of the nerves proximal to the site of needle insertion were assessed 5 days after injection. By changing the PPD concentration of solution for intraneural injection, various degrees of DTH reaction could be produced in the nerve. Infiltration of mononuclear cells including macrophages was observed around the vessels and in the vicinity of the myelin sheaths. Although nonspecific damage of axons, myelin sheaths and Schwann cells was observed in areas heavily infiltrated with inflammatory cells, primary demyelination was hardly recognized. Another group of Lewis rats previously immunized with galactocerebroside (GC), the major glycolipid hapten of myelin, in Freund's complete adjuvant received intraneural injection of PPD or GC liposomes. Neither cellular nor humoral immunity to GC was detected in these rats. The nerves injected with GC liposomes showed no inflammatory cell infiltration except for a few macrophages containing liposomes and those injected with PPD showed infiltration of mononuclear cells without primary demyelination. Our findings reveal that nonspecific myelin destruction induced by DTH does not play an important role in immune-mediated demyelination.

Animals↗
PubMed1986

Isolation and characterization of multilayered sheath membrane rich in glucocerebroside from shrimp ventral nerve.

A membrane fraction rich in glucocerebroside was isolated from homogenates of ventral nerves of pink shrimp (Penaeus duorarum) by sucrose gradient centrifugation. The membrane fraction was observed at 0.15 M sucrose and was rich in lipids (lipid/protein ratio approximately 15:1). Electron microscopy showed that the fraction was derived from myelin-like multilayered glial membrane ensheathing axons, which has morphological similarities to myelin. Most of the lipids in shrimp nerve, including glucocerebroside, sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and ethanolamine-plasmalogen, as well as cholesterol, appeared to be concentrated in this fraction. The fatty acids of these phospholipids were exclusively saturated or monounsaturated with C14-C26 chain lengths. The aldehyde moiety of plasmalogens contained only saturated C14-C18 carbon chains. Like glucocerebrosides, the sphingoid base of sphingomyelin consisted mainly of C14-C16 sphingenines and sphinganines, but they also contained significant amounts of C19 and C20 sphinganines. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins in this fraction showed several bands in the 23,000-85,000 Mr range. Radioimmunoassay, however, did not show cross-reactivity with antibodies to myelin basic protein. The functional role of this membrane in relation to mammalian myelin is discussed.

Animals↗
PubMed1986

Perforating pilomatricoma.

A case of "perforating pilomatricoma" is reported. A cutaneous horn-like tumor, the size of a grain of rice, developed on the left upper arm of a 51-year-old man. Histological examination revealed typical microscopic features of pilomatricoma. In addition, however, an interesting phenomenon was observed: a small portion of the mass had extruded from the upper dermis to the skin surface through the perforated epidermal channel. It might have been possible in this case for all of the tumor mass to be extruded by transepithelial elimination.

Humans↗
PubMed1986

Ultrastructural study of central nervous system demyelination in galactocerebroside sensitized rabbits.

The rabbit eye model was used to investigate the role of delayed type hypersensitivity and of circulating antigalactocerebroside antibody in primary demyelination. Delayed type hypersensitivity reaction to tuberculin was induced within the retinal myelinated layers by injecting purified protein derivative into the vitreous of rabbits that were previously immunized with complete Freund's adjuvant (CFA) alone, or galactocerebroside (GC) in CFA. The morphological features of the myelinated zones were assessed 5 days after injection. All rabbits showed infiltration of mononuclear cells within the myelinated fiber bundles. Rabbits previously sensitized with CFA alone showed nonspecific destruction of nerve fibers in severe inflammatory lesions, but primary demyelination was rarely found. This suggests that myelin destruction induced by delayed type hypersensitivity reaction to interstitial antigens (purified protein derivative, so-called "bystander demyelination") by itself does not play a major role in inducing primary demyelination. On the other hand, primary demyelination was recognized in the vicinity of infiltrating mononuclear cells in rabbits previously immunized with GC in CFA and had elevated titers of serum anti-GC antibody. The destruction of the blood-brain barrier was suggested by the presence of fibrin exudates in demyelinative lesions where observation revealed selective myelin breakdown such as vesicular disruption and active stripping of myelin. This study indicates that the anti-GC antibody is an important factor in central nervous system demyelination of GC-sensitized rabbits.

Animals↗
PubMed1985

Familial neuropathy with dementia, retinitis pigmentosa, and dysautonomia.

We studied a 59-year-old woman with dementia, retinitis pigmentosa, sensorimotor neuropathy, and attacks of vomiting associated with blood pressure lability and loss of consciousness. Abnormalities included CT evidence of cerebral atrophy, low IQ, slow central and peripheral nerve conduction velocities, axonal degeneration in sural nerve biopsy, and elevated levels of catecholamines and slow waves in EEG during attacks. Her sister, two brothers, and daughter also had progressive muscle weakness, visual disturbance, and similar vomiting attacks. The hereditary nervous system disorder does not fit any previously described condition.

Adult↗