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Biomedical subjects

T S Sahota

Publications and source records attributed to T S Sahota.

9 recordsLinked to original sources
PubMed1988

Image texture features for cell nuclei based on absorbance surface curvature.

The absorbance values of a cell nucleus, as measured at each pixel location, can be considered as forming a surface in a three-dimensional space. The principal curvatures of differential geometry can then be evaluated for this surface. From these curvatures, two new variables are computed that, when averaged over all pixels in the nucleus, are viewed as image texture measures. These two measures were found to be effective for differentiating insect populations and compared well with other features used in the TICAS system. This paper presents the results of comparing the performance of these two features with the performance of other features for several populations of cells.

Animals↗
PubMed1984

A computer-assisted cell identification system.

A computer and optical microscope image processing system for the acquisition, editing and analysis of quantitative, multivariate, cell nuclear data is described. The experimental equipment and methods are discussed, the mathematics used are presented, and the additions, modifications and deletions to the TICAS 11/45 set of computer programs for the analysis of the data are outlined.

Cell Nucleus↗
PubMed1983

A nonparametric approach to the discrimination of two cell populations.

An approach to the statistical testing of differences between two cell populations the elements of which are characterized by multivariate data is described. The approach is based on the Fisher discriminant and the Kruskal-Wallis test. The method, which is sufficient but not necessary, makes no assumptions about the normality of the data or about the equality of the covariance matrices for each population.

Animals↗
PubMed1982

Use of pectinase to dissociate plant nuclei for squash preparations: effect of hydration procedures.

Scale tissues taken from cones of pendent Douglas-fir Pseudotsuga menziesii (Mirb.) Franco were treated with pectinase to dissociate nuclei for Feulgen staining. Hydration of fixed tissues with water or a graded series of ethyl alcohol before pectinase treatment resulted in distention and faint staining of nuclei and inadequate disintegration of cell walls. These problems were overcome by adopting citrate buffer hydration. Analysis of digitized scans of nuclei provided information about the distribution of the stained material in density classes and nuclear size which is usually unavailable through the conventional double wavelength DNA measuring method.

Buffers↗
PubMed1981

Image enhancement for light microscopy.

Digitized light microscope images of low contrast histological samples were processed through image enhancement computer techniques. Original digital data and their square root transformations were linearly expanded to produce a wider range of brightness levels in the enhanced images. These treatments resulted in significantly improved images compared to those obtained by direct photomicrography.

Animals↗