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Biomedical subjects

T S Lie

Publications and source records attributed to T S Lie.

At least 55 records · Page 3Linked to original sources
PubMed1977

[Suppression of the cell-mediated immunity in minor histoincompatible rat kidney allograft recipients (author's transl)].

To study the immunological status of recipients of major compatible and minor incompatible kidney allografts, we transplanted FiS and FLF1 kidneys into LEW rats. Most of the FiS kidneys were rejected within 55 days. Of 24 recipients, only 4 survived longer than 4 months. However, two-thirds of the FLF1 recipients survived longer than 4 months. The other third died with 64 days. During the first postoperation week a high level of lymphocytotoxin was detected in the serum of the FiS kidney recipients. Thereafter hardly any alternation of its titer was found, and no variation among the recipients of major histocompatible kidney allografts was shown. The FLF1 kidney recipients showed a low titer of antibody. The hemagglutinin titer showed the same trend as the lymphocytotoxin titer. A blocking serum factor could not be found in the serum of the kidney recipients with the microcytotoxity assay method or with the allorosette-formation inhibition test. Cellular immunity, which was studied with the GvH-reaction and microcytotoxity assay, was detected in the first postoperative week. However, this immunity was gradually supressed, and after 6 weeks was no longer to be found. This immunological status remained unchanged in the indefinitive surviving kidney-recipients in spite of antigen inoculation with two skin allografts of donor origin. This immunological status could be defined as "graft acceptance".

Animals↗
PubMed1977

[Active enhancement of canine liver allograft by pretreatment with polyspecific spleen alloantigen (author's transl)].

Because it is not possible to use donor specific antigens for the induction of immunological enhancement in cadaveric organtransplantation, attempts were made to use polyspecific antigens in the enhancement of orthotopic canine liver allotransplants. Of 34 mongrel recipients, 17 controls survived for 6.9 +/- 1.5 days. Six recipients (group 3) were given 750 mg/kg polyspecific, semisoluble antigen prepared from 20 spleens (PSEA 20) together with 10 mg/kg prednisolone on days 15, 8 and 1 before transplantation. The mean survival time of this group was 10.1 +/- 2.0 days (P less than 0.01 compared with the control group). Six other recipients (group 4) were treated similiarly, except that the antigen had been prepared from a pool of 70 spleens (PSEA 70). Survival was variable here: 3 survived for more than 3 weeks and the other 3 died on days 1, 7 and 8 post-operatively, with signs of accelerated rejection. Donors and recipients were not identical for LD determinants, but one donor recipient pair with near identity showed a higher degree of enhancement. The recipients displaying accelerated rejection had markedly higher lymphocytotoxic and haemagglutinating antibodies. Animals surviving for longer periods had low antibody titres. In addition, all recipients progressive rejection were found to show inhibition of leucocyte migration. After three antigen doses rosette-forming lymphocytes were present in increased numbers in peripheral blood, and remained unchanged thereafter.

Animals↗
PubMed1977

Detection of serum-blocking factors by inhibition of allorosette formation in rats with long-surviving renal allografts following short-term postoperative ALS treatment.

Thirty-nine (LEW x BN)F1 kidneys were transplanted to LEW rats. Twenty-four untreated recipients survived for a mean time of 16.1 +/- 1.7 days (group 1). Fifteen recipients received 4 ml of antilymphocytic serum per rat (group 3). In the last group 10 recipients survived for more than 4 months. The spleen cells of these permanently surviving 10 rats were obtained by splenectomy and used in a graft-versus-host assay, and this assay showed that the reactivity of these cells was normal. Following splenectomy the animals were given an (LEW x BN)F1 skin allograft, followed 18 days by a second. After another 18 days (LEW x Buf)F1 "third party" skin allografts were transplanted to the same animals. Animals of group 2 rejected their first grafts with a mean survival time of 12.2 +/- 1.2 days, whereas the second grafts were rejected normally as were the third party grafts. Attempts were made to detect lymphocytotoxic antibodies and haemagglutinins before and after the transplantation of skin grafts and none could be found up to day 53. The sera of group 2 inhibited allorosette formation by 38%. This serum-blocking factor was donor specific. It is probable that the survival of the kidney transplants following antilymphocytic serum treatment was brought about by the development of blocking antibodies.

Animals↗
PubMed1977

[Is permanent survival of kidney allograft receivers in weak histo-incompatible combinations dependent on enhancement?].

To study the immunological status of indefinitely surviving recipients of histocompatible (minor histoincompatible) allografts we transplanted Fi or (Fi x LEW)F1-kidneys to LEW-rats. At the same time bilateral nephrectomy was performed. To examine the cellular immune response we carried out local GvHR, microcytotoxicity assay and allorosette-formation test with recipient cells. We also studied lymphocytotoxins in the serum of recipients. To detect a blocking serum factor we used allorosette-formation inhibition test and microcytotoxicity assay. A blocking serum factor could not be found. In spite of stimulation with donor specific skin graft the cellular immune response of prolonged surviving recipients was inhibited. Our results suggest that prolonged survival of minor histoincompatible renal allograft recipients was caused by suppression of cellular immune response.

Animals↗
PubMed1976

[Treatment of comatose patients by extracorporeal hemoperfusion with activated charcoal].

Hemoperfusion with activated charcoal is indicated in intoxications and in hepatic comma because the charcoal absorbs toxic substances. Good results have been obtained so far with activated charcoal liver perfusion in barbiturate intoxications, but less satisfactory results in hepatic comma. The indication for perfusion should also be made in clinically hopeless cases. The principal complications with activated charcoal perfusion are a decrease in thrombocytes and microemboli of charchoal particles. These complications can be avoided by coating the charcoal particles. A flow-rate of 200-300 ml/min is best in humans because this flow-rate ist guaranteed by an AV shunt in the forearm.

Capsules↗
PubMed1976

[Immune status of indefinitely surviving rat renal allograft recipients after short course of ALS-treatment Detection of a serum blocking factor by allorosette formation inhibition test (author's transl)].

53 LBNF1 kidneys were grafted into L-rats. 24 untreated recipients served as control and survived 16.1 +/- 1.7 days. 14 recipients were treated with 4 ml alloantiserum (ADS) each, and the other 15 animals with 4 ml ALS each. 9 and 10 of these 14 and 15 animals survived for more than 4 months. We performed GvH-reaction with spleen cells of these 19 animals. It showed normal cellular immune response. After splenectomy first donor specific skin was transplanted, 18 days later second skin of same origin (LBNF1) and 18' days after third party skin (LBufF1). The first grafts survived 13.1 +/- 1.9 days in the recipients of ADS treatment and 12.2 +/- 1.2 days in the recipients of ALS. Second skin grafts were rejected delayed in the former recipients as the first grafts, in the later more accelerated than the first grafts. The third grafts survived as the controls. Before and after skin grafting we could not detect lymphocytotoxin and hemagglutinin. The sera of animals with ADS therapy inhibited the spontaneous allorosette formation to 46.0 +/- 4.4% and of ALS therapy 37.7 +/- 9.2%. Our results suggest that after ALS treatment blocking antibodies were produced in the recipients and they cause the enhancement of kidney allograft.

Animals↗
PubMed1976

Active enhancement of rat kidney allografts. Effect of pretreatment with prednisolone and donor-specific antigen.

(Lewis x Brown Norway) F1 hybrid rat kidney allografts were transplanted to bilaterally nephrectomized Lewis recipients pretreated in various ways. The mean survival time of untreated controls was 16.1 +/- 1.7 days. All rats pretreated with 1.67 g/kg of semi-soluble Brown Norway spleen extract and 5 mg/kg of prednisolone on days 15, 8, and 1 before transplantation survived indefinitely. Pretreatment with semi-soluble or soluble extract alone prolonged survival modestly (36.5 +/- 13.6 and 30.8 +/- 5.6 days, respectively), but the former induced indefinite survival in two of eight animals. Prednisolone on its own failed to bring about prolongation of survival and the combined use of soluble extract and prednisolone did not reveal a synergistic effect. Cytotoxic antibody titres in animals showing indefinite survival were very low, and there was no correlation between antibody titres and prolonged survival. It is assumed that the pretreatment with semi-soluble extract and prednisolone inhibited the formation of cytotoxic antibodies as well as cell-mediated immunity, and encouraged the formation of enhancing antibodies. To study the cellular and humoral reactivity of five prolonged survived kidney recipients, 1st and 2nd donor-specific skin grafts were carried out. The humoral and cell-mediated responses were somewhat delayed in these recipients but otherwise normal except for the absence of the second-set phenomenon.

Animals↗
PubMed1976

[Treatment of hepatic coma using extracorporeal perfusion with baboon and human liver as well as with activated charcoal].

In the 6 patients with hepatic coma hemoperfusions with 8 baboon and 2 human livers were carried out. Four awoke from the coma, one women could be discharged, but the others died of various complications and liver insufficiency. In the 4 patients with hepatic coma, 11 hemoperfusions with activated charcoal columns were performed. Two awoke from the coma, but they died of liver insufficiency. We were able to detect the elevation of preforming antibodies in a discharged patient.

Acute Disease↗
PubMed1976

[Active enhancement of canine kidney allografts (author's transl)].

In 32 beagles heterotopic renal allotransplantations and bilateral nephrectomies were carried out. Control animals (5 dogs) survived 9.4 +/- 1.6 days. 22 recipients were pretreated with donor-specific semisoluble spleen antigen; 5 recipients pretreated with the antigen alone (780 mg/kg body weight) survived 16.6 +/- 2.3 days. A pretreatment with antigen combined with 5 mg/kg body weight Prednisolone resulted in no significant prolongation of survival time (5 animals), but 5 dogs survived significantly longer after pretreatment with the antigen and 50 mg/kg body weight Prednisolone (27.4 +/- 2,7 days). In 5 recipients after preoperative application of 3 doses Prednisolone alone (50 mg/kg body weight each) no prolongation of survival time could be observed (10.2 +/- 0.7 days). We found no correlation between lymphocytotoxic antibody titre and survival time.

Animals↗
PubMed1976

Prolongation of allograft and xenograft survival by pretreatment with prednisolone and donor-specific antigen.

Sprague-Dawley rats were grafted with skin of Wistar rats. Surprisingly, pretreatment with donor-specific spleen homogenate and semisoluble antigens combined with prednisolone resulted in a prolonged survival of the skin grafts. The same effects were observed after transplantation of rabbit skin to Sprague-Dawley rats. Lewis rats were grafted with kidneys of LewisX brown-Norway F1 rats and simultaneously subjected to bilateral nephrectomy. The recipients were pretreated with soluble and with semisoluble donor-specific antigen. The recipients survived in definitely after pretreatment with semisoluble antigen combined with prednisolone. Combined administration of soluble antigen and prednisolone was ineffective. In the canine kidney allografts, the recipients were pretreated with semisoluble donor-specific spleen antigen; in the canine liver allografts, they were given soluble and semisoluble antigen. The canine kidney recipients survived significantly longer when they had been pretreated with antigens combined with high doses of prednisolone. Pretreatment with high doses of prednisolone alone did not prolong the survival of kidney grafted rats and dogs, and pretreatment with antigen alone had no effect on the survival of kidney grafted dogs. Pretreatment with antigen alone had no effect on the survival time of liver allografts in dogs, but the recipients of the liver grafts survived longer than and controls--some of them for more than 1,200 days--when they had been pretreated with antigens combined with prednisolone. In this respect, the semisoluble antigen was more effective than the soluble form. After three spaced antigen injections, the lymphocytotoxic antibody titers on the day of the grafting varied, but they were always low. There was no correlation between the titers on the day of transplantation and the duration of survival. Recipients pretreated with antigen showed lower postoperative antibody titers than those without antigen.

Animals↗
PubMed1976

Active enhancement of canine liver allografts--effect of pretreatment with prednisolone and donor-specific antigen.

We investigated the active enhancement of liver allografts in 44 pairs of beagle dogs by pretreatment with semisoluble and soluble donor-specific spleen antigens. If the antigen were administered together with prednisolone, time of survival was significantly prolonged--with soluble antigen by 36.8 +/- 5.9 days and with semisoluble antigen by more than 3 years. Antigen pretreatment alone or antigen in combination with postoperative treatment of minimal immunosuppressive drugs had no significant effects. We found no correlation between the titer of lymphocytotoxic antibodies in the serum of recipients at the time of transplantation and survival times.

Animals↗
PubMed1976

[Demonstration of blocking factors in permanently surviving rat-kidney allograft recipients after short postoperative treatment with alloantiserums].

GvHR tests were performed on spleen cells of 9 recipients surviving rat renal allografts for more than 120 days. The recipients had undergone a short term ALS-treatment. The GvHR tests showed normal cell-mediated immunity. After splenectomy and again 18 days later LBNF1 skin was grafted to the renal recipients. After a further period of 18 days LBufF1 skin was grafted. After some delay the specific grafts were rejected. The unspecific skin was rejected in a normal way. Before and after skin grafting the authors could not find the lymphocytotoxin and hemagglutinin. Instead a blocking factor which inhibited the spontaneous allorosette formation was detected in the sera of recipients. The authors compared the results with passive enhanced renal recipients. The two groups did not differ significantly from each other.

Animals↗
PubMed1975

[The effect of donorspecific antigen type for active enhancement of renal-allografts in the histoincompatible inbred rats (author's transl)].

49 kidneys of male LBNF1-rats were transplanted into male Lewis-rats. BN-rats were used as a source of antigen. Control animals survived 16.1 +/- 1.8 days. 5 recipients were preteated with donorspecific living cells, 7 with semisoluble and 5 with low dosage of soluble antigen. The living cell-pretreatment showed the best effect for the active enhancement, the soluble antigen the least. The pretreatment with 6 mg protein/kg bw. caused the sensibilization of recipients. At the time of transplantation lymphcytotoxic antibody titers were detected in all recipients with pretreatment of living cells, but no titer in other groups. Renal allografts with elevated postopertive titer were rejected rapidly.

Animals↗