Unusual and very long-chain fatty acids produced by Basidiomycetes.
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Basidiomycota↗
Biomedical subjects
T Rezanka
Publications and source records attributed to T Rezanka.
Fatty acids and production of tylosin-like compounds in Streptomyces fradiae.
The composition of fatty acids and the spectrum of macrolide antibiotics produced in 7 mutant strains of Streptomyces fradiae, a tylosin producer, were investigated. The strains under investigation differed in the production level and representation of individual tylosin-like compounds. The composition of fatty acids in the mycelium did not depend on the total production. However, the strains producing relomycin in addition to tylosin produced a significantly higher fraction of fatty acids with a higher melting point, and, on the contrary, the strains producing only tylosin or tylosin and desmycosin synthesized a significantly lower proportion of these acids. The results obtained indicate that in addition to the activity and substrate specificity of secondary metabolism enzymes, the composition of the tylosin-like compounds produced can be influenced by the cell membrane and its function.
Fatty Acids↗
Very-long-chain fatty acids from lower organism.
The qualitative occurrence and quantitative proportion of very-long-chain fatty acids (above C22), mainly in lower organisms and briefly in higher plants and animals is described.
Animals↗
Effect of clomiphene on the content of sterols and fatty acids in Saccharomyces cerevisiae.
During cultivation of Saccharomyces cerevisiae clomiphene regulates both quantitative and qualitative production of sterols and fatty acids as identified by gas chromatography and mass spectrometry. The content of sterols decreases to 75%, the production of fatty acids is comparable with that in the control. The occurrence of sterols increases; sterols with methyl group in position 4, without double bond in position 22 and with double bond in position 24(25) or 24(28) predominate. Among fatty acids shorter saturated and monoene acids are primarily produced, 2-hydroxy acids practically disappeared.
Clomiphene↗
Estimation of lipase activity by the diffusion plate method.
A diffusion plate method for the assay of the activity of lipases in large series of samples was worked out. When using this method the maximum deviation was found to be +/- 3.8%.
Animals↗
Fatty acids of Streptomyces cinnamonensis, producer of monensin.
Using the gas chromatography-mass spectrometry (GC-MS) method we found in Streptomyces cinnamonensis saturated fatty acids of iso- and anteiso- types, cyclopropyl acids, and unsaturated fatty acids where the double bond position was determined by MS of their pyrrolidine derivatives.
Fatty Acids↗
Optimization of streptomycete strains producing polyether and macrolide antibiotics.
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Amino Acids↗
Hydrocarbons in green and blue-green algae.
Liquid column chromatography and thin-layer chromatography were used to determine the total content of hydrocarbons and gas chromatography was used to evaluate composition of hydrocarbons in green algae (Chlorella kessleri, C. vulgaris, Chlorella sp., Scenedesmus acutus, S. acuminatus, S. obliquus) and the blue-green alga (Spirulina platensis) cultivated under autotrophic or heterotrophic conditions. In C. kessleri cultivated under heterotrophic conditions the content of hydrocarbons was found to be about 10(-2)% (per dry mass), whereas under autotrophic conditions it was about 10(-3)% (per dry mass). The highest content of hydrocarbons was detected in species of the genus Scenedesmus cultivated autotrophically (10(-1)%). Heptadecane and hexacosane were found as major alkanes, 1-heptadecene was detected among alkenes.
Alkanes↗